Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Rhus Trichocarpa Suppresses IgE-mediated Allergic Response In Vitro and In Vivo

개옻나무 추출물의 IgE 매개성 알레르기 반응 억제 효과 및 기전

  • Lim, Hannah (College of Pharmacy, Duksung Women's University) ;
  • Kim, Young Mi (College of Pharmacy, Duksung Women's University)
  • 임한나 (덕성여자대학교 약학대학) ;
  • 김영미 (덕성여자대학교 약학대학)
  • Received : 2013.02.06
  • Accepted : 2013.04.09
  • Published : 2013.06.30
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Abstract

Mast cells is the key effector cells for IgE-mediated allergic responses. In this study, we investigated whether Rhus trichocarpa extract (RT) inhibited IgE-mediated allergic responses in mast cells and an allergic animal model. We further tried to find its mechanism of action in mast cells. We found that RT suppressed antigen-stimulated degranulation and production of TNF-${\alpha}$ and IL-4 in rat basophilic leukemia (RBL)-2H3 mast cells and bone marrow-derived mast cells (BMMC), as well as IgE-mediated passive cutaneous anaphylaxis (PCA) in mice. As the mechanism of action of RT, it inhibited the activation of spleen tyrosine kinase (Syk), a pivotal signaling molecule for activation of mast cells and that of LAT, a downstream adaptor molecule of Syk in $Fc{\varepsilon}RI$-mediated signal pathways. RT also suppressed the activation of mitogen-activated protein (MAP) kinases and Akt. The current results demonstrated for the first time that RT has the anti-allergic effect through inhibiting degranulation and secretion of cytokines by suppression of Syk in antigen-stimulated mast cells. Therefore, RT might be useful for allergic diseases.

Keywords

References

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