• Fisheries and Aquatic Sciences
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• v.26 no.10
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• pp.605-616
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• 2023

Scuticociliatosis, caused by the parasitic pathogen Miamiensis avidus, poses a significant threat to olive flounder farms in South Korea. Infected fish suffer from severe systemic infections affecting various organs, with potential secondary bacterial diseases. This study investigated the emergence of different M. avidus serotypes in 20 olive flounder farms on Jeju island, South Korea, from 2015 to 2020. Additionally, we identified Tenacibaculum maritimum as a co-infecting bacteria. Based on serotyping and monitoring data, we developed a trivalent vaccine targeting two serotypes of M. avidus and one strain of T. maritimum. The efficacy of the vaccine was evaluated under laboratory conditions and demonstrated a relative percentage of survival (RPS) of 75%, 80%, and 93% for M. avidus serotype I, M. avidus serotype II and T. maritimum, respectively. Furthermore, successful field trials conducted on four different olive flounder farms resulted in significantly higher survival rates (52%-76% RPS) and weight gains in vaccinated fish. Overall, this study presents an effective vaccine against M. avidus and T. maritimum infections in farmed olive flounder, making a valuable contribution to sustainable aquaculture in South Korea.

• 한국신재생에너지학회:학술대회논문집
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• 2010.06a
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• pp.151.2-151.2
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• 2010

우리나라는 "제 3차 신재생에너지 기술개발 및 이용 보급 기본계획"을 통해 2030년까지 111.5조 원을 투자하여 전체 에너지의 11%를 신재생에너지로 공급한다는 목표를 설정하였다. 그러나 신재생에너지는 기존의 원자력이나 화석에너지에 비하여 생산비용이 높아 보조나 융자 등의 정부지원에 의존하여 보급이 이루어져왔다. 신재생에너지 보급확대 및 산업발전을 위한 보급정책의 일환으로 정부는 2012년부터 RPS(Renewable Portfolio Standard, 신재생에너지 공급의 무화제도)를 도입키로 확정하였다. RPS의 도입은 일정규모 신재생에너지 시장수요를 창출함과 동시에 신재생에너지원간 가격경쟁 구도 형성의 유인이 될 수 있다. 이는 전력가격 일괄상승 및 녹색가격제도(Green Pricing) 등의 정책적 논의로 이어질 수 있다. 따라서 소비자 측면에서 신재생에너지 전력의 가치를 어떻게 평가하는지를 분석하여 RPS 시행제반의 정책적 논의의 기초자료를 마련할 필요가 있다. 특히 RPS는 신재생에너지원 간의 경쟁을 가능하게 하므로 개별 신재생에너지원에 따라 소비자 선호의 차이가 어떻게 나타나는지 연구되어야 한다. 이에 본 연구는 환경재 혹은 비시장재화의 가치추정에 가장 널리 활용되고 있는 조건부가치평가법(Contingent Valuation Method; CVM)을 적용하여 풍력, 태양광, 수력으로 생산한 전력에 대한 소비자의 지불의사액(Willingness to Pay; WTP)을 분석하는 모형을 수립하였다. 이를 위해 Zografakis et al.(2010), Yoo and Kwak(2009), 이창훈 황석준(2009), Nomura and Akai(2004), Bately et al.(2001) 등의 선행연구를 참조하여 신재생에너지 전력 가치의 설문에서 고려되어야 하는 요인들을 선정하였다. 이를 토대로 설문 시나리오를 작성하여 각 요인들이 신재생에너지 전력에 대한 지불의사액 결정에 어떠한 영향을 미치는지 분석가능한 모형을 설정하였다. 뿐만 아니라 기존 연구들이 신재생에너지로 생산된 일반적인 전력에 대한 지불의사액을 질문하는데 그쳤다면, 본 연구에서는 각 원별로 지불의사액의 차이를 도출할 수 있는 설문모형을 구축하는데 중점을 두었다. 본 연구결과는 향후 설문수행을 통하여 신재생에너지원별로 소비자의 선호를 분석할 수 있는 연구로 발전될 수 있으며, 이는 RPS 도입으로 인한 전력가격 정책 수립의 기초 연구자료로 활용될 수 있다 하겠다.

• Journal of fish pathology
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• v.19 no.1
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• pp.73-82
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• 2006

Streptococcal infections were considered as a serious problem because of significant economic losses in fish farm industry. We evaluated the efficacies of Streptococcus iniae vaccines in olive flounder, Paralichthys olivaceus. The vaccines were prepared from 10% neutral buffered formalin to give a final concentration of 0.3% or 3%, respectively. Fish were immunized by intraperitoneal injection of the experimental vaccines once or twice. Neither of the vaccines gave rise to any significant side effects. The antibody titers of booster immunized groups were significantly higher than those of prime immunized groups with both of the vaccines. According to formalin dosage, significantly increased antibody titers were produced by 3% formalin-killed cells (FKC) at 4weeks and 8weeks after prime and booster vaccination, respectively. Although the different levels of antibody production were showed by the vaccinated fish, the good protection obtained in challenge trials of the both vaccines. Fish immunized with 0.3% FKC once or twice had the relative percent survival (RPS) of 66.7% and 87.5%, respectively. Similarly, fish immunized with 3% FKC once or twice had the RPS of 70.0% and 77.0%, respectively. Further experiments are needed to study not only correlation between the antibody titers and RPS against S. iniae but also the side effects of high dose of formalin on antigenicity.

• Journal of the Korean GEO-environmental Society
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• v.11 no.5
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• pp.5-13
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• 2010

The research is intended to develop and verify a biological complex soil treatment process to treat and restore soil and groundwater which is contaminated with oil, heavy metals, and nutrients through experiments with the series of treatment process such as bioreactor, rolled pipe type of contact oxidation system(RPS), and chemical processing system. 5 microbial strains were separated and selected through experiment, whose soil purification efficiency was excellent, and it was noted that anion- and nonion-series of complex agent was most excellent as a surfactant for effectively separating oils from soils. Method to mix and apply selected microbes after treating the surfactant in the contaminated soil was most effective. The removal efficiencies of total petroleum hydrocarbon (TPH)-contaminated soil about 5,000mg/L and above 10,000mg/L were approximatly 90.0% for 28 days and 90.7% for 81 days by soil remediation system and the average removal efficiencies of BOD, $COD_{Mn}$, SS, T-N, and T-P in leachate were 90.6, 73.0, 91.9, 73.8, 65.7% by the bioreactor and RPS. The removal efficiency was above 99.0% by chemical processing system into cohesive agents.

• Korean Journal of Plant Resources
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• v.25 no.1
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• pp.142-151
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• 2012

This study was carried out to evaluate genetic variation of early-heading rice (Oryza sativa L. cv. Dongjin 1) lines derived from gamma-ray ($^{60}Co$, 300 Gy) irradiation. The average heading date of the 5 early-heading lines in $M_7$ and $M_8$ generation was faster than that of untreated control as 11 (line ${\gamma}$-2), 10 (line ${\gamma}$-5), 6 (${\gamma}$-1 line), 5 (${\gamma}$-3) and 4 days (line ${\gamma}$-4), respectively. According to ISSR analysis, polymorphic rate of the early-heading lines (from 5.9% to 23.4%) was higher than that of control (4.3%). The result indicates that the gamma-ray promote variation at DNA level. When genetic variations of rps16-trnK region were evaluated by nucleotide analysis, nucleotide length of the rps16-trnK region was 664 bp in all the early-heading lines and control. Out of 5 sites of nucleotide transposition detected in the region, however, 2 sites were appeared only in the early-heading lines.

• Journal of Animal Science and Technology
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• v.63 no.5
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• pp.1126-1141
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• 2021

Recent evidence has shown that methionine (Met) supplementation can improve milk protein synthesis under hyperthermia (which reduces milk production). To explore the mechanism by which milk protein synthesis is affected by Met supplementation under hyperthermia, mammary alveolar (MAC-T) cells were incubated at a hyperthermic temperature of 42℃ for 6 h in media with different concentrations of Met. While the control group (CON) contained a normal amino acid concentration profile (60 ㎍/mL of Met), the three treatment groups were supplemented with Met at concentrations of 10 ㎍/mL (MET70, 70 ㎍/mL of Met), 20 ㎍/mL (MET80, 80 ㎍/mL of Met), and 30 ㎍/mL (MET90,90 ㎍/mL of Met). Our results show that additional Met supplementation increases the mRNA and protein levels of BCL2 (B-cell lymphoma-2, an anti-apoptosis agent), and decreases the mRNA and protein levels of BAX (Bcl-2-associated X protein, a pro-apoptosis agent), especially at an additional supplementary concentration of 20 ㎍/mL (group Met80). Supplementation with higher concentrations of Met decreased the mRNA levels of Caspase-3 and Caspase-9, and increased protein levels of heat shock protein (HSP70). The total protein levels of the mechanistic target of rapamycin (mTOR) and the mTOR signalling pathway-related proteins, AKT, ribosomal protein S6 kinase B1 (RPS6KB1), and ribosomal protein S6 (RPS6), increased with increasing Met supplementation, and peaked at 80 ㎍/mL Met (group Met80). In addition, we also found that additional Met supplementation upregulated the gene expression of αS1-casein (CSN1S1), β-casein (CSN2), and the amino acid transporter genes SLC38A2, SLC38A3 which are known to be mTOR targets. Additional Met supplementation, however, had no effect on the gene expression of κ-casein (CSN3) and solute carrier family 34 member 2 (SLC34A2). Our results suggest that additional Met supplementation with 20 ㎍/mL may promote the synthesis of milk proteins in bovine mammary epithelial cells under hyperthermia by inhibiting apoptosis, activating the AKT-mTOR-RPS6KB1 signalling pathway, and regulating the entry of amino acids into these cells.

• 한국신재생에너지학회:학술대회논문집
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• 2005.11a
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• pp.519-529
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• 2005

국내에서 시행중인 발전차액지원제도는 신재생에너지의 보급 확대를 목적으로 2002년부터 시행 중에 있으며, 법적으로 인증된 신재생에너지 발전전원에 대하여 일종의 우대가격인 기준가격을 적용하고 있다. 2002. 5월에 최초 고시된 기준가격지침 고시기준은 그동안 두 차례의 개정을 거친 바 있으며, 2006년 10월경에는 전면 개정을 앞두고 있다. 향후, 개선될 기준가격 시스템은 국내의 현실적인 여건 등을 감안하여 대상 전원별로 고정, 또는 선택적인 요금제를 통하여 탄력적인 기준가격체계를 적용하는 방향으로 검토할 예정이다.

• Proceedings of the KIEE Conference
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• 2011.07a
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• pp.1340-1341
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• 2011

'12년 RPS 제도시행으로 태양광발전이 신재생에너지로서 큰 관심을 받고 있다. 이에 홍수기 이외에는 대규모의 유휴지가 발생하는 홍수조절 부지를 활용하여 태양광전원 개발방안과 개발규모에 관하여 검토하는 것이 본 연구의 목적이다.

• Proceedings of the Korean Information Science Society Conference
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• 2000.10a
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• pp.486-488
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• 2000

본 연구는 정형기법을 사용하여 Safety-Critical System의 개발 방법론을 제시한다. Safety-Critical System의 전체적인 개발 과정을 제시하고 Safety-Critical System 중의 하나인 원자력 발전소 시스템 중 Reactor Protection System(RPS)을 정형 명세(Formal Specification)하고 정형 검증(Formal Verification)하는 과정과 그에 따른 각 과정의 Compliance를 확인하는 예를 든다. 여기서 정형 명세에는 Software Cost Reduction(SCR)이하는 도구가 사용되었고, 정형 검증에는 SPIN이, Compliance를 확인하는 데에는 Prototype Verification System(PVS)를 사용하였다.

• Proceedings of the KIEE Conference
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• 2004.11b
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• pp.290-292
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• 2004

Generally, there are two types of regulation strategies for encouraging renewable energies ; regulation by quantity and price. With examples of quantity regulation, there RPS (Renewable portfolio standard) system in United States America, Renewable obligation in England, and MRET system in Australia. Countries that chose the price regulation are Germany, Spain, France, Portugal. This Paper overviews the current trends of regulation system on policies for renewable energies in foreign countries on these days.