• 사상체질의학회지
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• 제1권1호
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• pp.139-151
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• 1989

In order to investigate the effects of Raw, Dried and Steamed Roots of Rehmanniae Radix (R.R.: from Wonju province, Korea) on cell-mediated arid humoral immune response, the author performed this experimental study. Delayed type hypersensitivity (DTH) and rosette forming cells (RFC) for cell-mediated immune response, hemagglutinin (HA) titers, hemolysin (HL) titers were maeasured in ICR mice. The results were summarized as follows: 1) DTH was increased with the order of Steamed Roots of R.R., Raw Roots of R.R., Dried Roots of R.R.-treated group, as compared with the control group, with statistical significance. 2) RFC were increased with the order of Raw Roots of R.R., Steamed Roots of R.R., Dried Roots of R.R.-treated group, as compared with the control group, with statistical significance. 5) HA titers were increased with the order of Steamed Roots of R.R., Row Roots of R.R., Dried Roots of R.R.treated group, as compared with the control group, with statistical significance. 4) HL. titers were increased with the order of Raw Roots of R.R., Steamed Roots of R.R., Dried Roots of R.R.-treated group, as compared with the control group, with statistical significance. Through in the experimental study in ICR mice, these findings suggest that all of the treated group was increased in cell-mediated immune reaponse, Raw, Steamed Roots of R.R. were increased more as compared with the Dried Roots of R.R., and all of the treated group was increased in humoral immune response, Raw, Steamed Roots of R.R. were increased more as compared with the dried Roots of R.R.

• Plant Resources
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• 제6권1호
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• pp.1-6
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• 2003

Ginseng(Panax ginseng C. A. Meyer) is important medicinal plant but requires 4-year cultivation for root harvest because of slow growth. In contrast, ginseng hairy roots induced by introducing Ri-plasmid of Agrobacterium rhizogenes into genomic DNA of plant cells show vigorous growth, and the hairy roots produce the same or more saponins than natural ginseng roots. Therefore, hairy roots can be used for commercial purposes. The present study was carried out to induce hairy roots with both active growth and high saponin contents. Numerous hairy roots of Panax ginseng were obtained after root disks of three-year old roots were infected with Agrobacterium rhizogenes R1000 A4T in dark condition after one month of culture. About 3 hundred lines of hairy roots were selected according as morphological characters on medium with carbenicillin. After pre-selection of fifteen lines of hairy roots with active growth, KGHR-l and KGHR-8 lines were finally selected which had characters of high content of ginsenoside-Rd and ginsenoside-Re, respectively. The optimum growth of hairy roots was achieved in the culture of 1/2 MS liquid medium in dark (22 $^{\circ}C$) under 60 rpm gyratory shaking. Hairy roots grew well in 5L Erlenmeyer flasks, lL roller drums, 10L jar-fermenters, and especially in 20L air-lift culture vessels.

• KSBB Journal
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• 제4권3호
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• pp.288-292
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• 1989

인참 근 조직에 Agrobacterium rhizogenes strain $A_4$를 접종하여 hairy roots 유도와 유도된 hairy roots의 배양 조건을 조사하였다. 48시간 배양된 균($2{\times}A92$bacteria/ml)을 접종하여 암조건($26{pm}1^{\circ}C$)하에서 배양하였을때 6-7주후 tumor가 형성되었으며, 10-12주후 hairy roots가 유도되었다. 근 년별 hairy roots 유도율은 5년근이 4, 6년근에 비하여 높았으며, 배지에 IAA, 2, 4-D, IBA 및 tryptophan 을 각각 첨가시켰을때 15-30mg/l tryptophan에서 tumor 및 hairy roots 유도율이 증가되었음을 나타내었다. 또한 유도된 hairy roots hormone-free인 RCM배지(sucrose 3%, pH 4.5)에서 배양하였다.

• Mycobiology
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• 제28권1호
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• pp.27-32
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• 2000

The ectomycorrhizal roots were collected from the soils around the bases of basidiocarps of the four edible mushrooms in the stands of Pinus densiflora or Querus acutissima communities (Mt. Wol-Ak in Eastern Chung-Puk): The basidiocarps of Tricholoma matsutake (TM), Sarcodon asparatus (SA), S. imbricatum (SI), and Polyozellus multiplex (PM) are usually collected. The ectomycorrhizal roots of TM, PM and SI were related to the roots of P. densiflora, but the other to the roots of Q. acutissima in Korea. Particularly, the basidiocarps of PM were collected in the mixed stand of both P. densiflora and Q. acutissima. The morphologies of the ectomycorrhizal roots were observed to be the yellowish brown coral (dichromatous) or pyramid types in the roots of the pine, but dark brown un-branched sticks (roots) in the ends of ectomycorrhizal roots of Querus plants. The un-branched roots were covered with the dark mycelia (rhizomorph) around them. Therefore, the ectomyorrhizal roots of PM were observed to have two kinds of types; The single blackish un-branched roots were observed to be attached to the yellowish coral type roots. The bundles of TM mycelia were filled with cortical cells (in the roots of P. densiflora), but the mycelia of the other fungi (Aphylloporales) were massed between the cortical cells of P. densiflora or Q. acutissima. Their anatomical and gross features were considered to be simailar but very important in the ectomycorrhizal roots for these edible mushrooms.

• 한국작물학회지
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• 제27권3호
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• pp.193-197
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• 1982

수도의 동일요소에 형성된 각 관근의 신장량은 다음과 같은 관계에 있음을 알았다. 1. 분벽아를 제거하여 각 관근만을 sink로 하였을 경우에는 중륵의 가까운 곳에 형성된 관근이 분벽아쪽에 형성된 관근보다 신장량이 좋았다. 2. 분벽아가 관근과 같이 sink로서 존재할 경우, 전출엽요소에서 출근한 관근이 가장 길었으며 또 분벽경 부근에 형성된 관근이 그 다음이었고 중륵 쪽에 형성된 관근이 가장 짧았다.

• 생약학회지
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• 제48권1호
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• pp.51-55
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• 2017

Aralia continentalis Kitagawa (Araliaceae), known as "Dokwhal" in Korea, has been widely used in traditional Korean medicine for analgesia, neuralgia, sweating, and rheumatism. The biological activity was estimated with methanol extracts of from cultivated roots and adventitious roots of A. continentalis. DPPH and ABTS activities showed the highest activity in methanol extract of adventitious roots at 175.6 and $279.7{\mu}g/mL$ ($RC_{50}$), respectively. Antioxidant activity of methanol extract of the adventitious roots was higher than other samples. In the antibacterial activity assay (paper disc method), the methanol extract of adventitious roots showed activity against S. aureus, S. epidermidis, B. subtilis, and S. enterica. This study demonstrated that adventitious roots of A. continentalis, which was produced for the first time in the roots of A. continentalis, can be used as a natural antioxidant and antibacterial agents.

• 동아시아식생활학회지
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• 제22권3호
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• pp.350-356
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• 2012

The purpose of this study was to make Korean yaksun chicken sauce with milk vetch roots and angelica roots. Four different amounts of angelica roots were added to chicken sauces, and general characteristics were measured. The pH values of sauces increased with decreasing Angelica root content. On the other hand, sugar contents, solid contents, and lightness of sauces decreased with decreasing Angelica root content. In a palatability test, sauces with 1 g and 2 g of angelica roots scored high in color, taste, flavor, and overall acceptability. Microbial cell counts of sauces were not significantly different according to the amount of angelica roots. Further, hydroxyl radical scavenging activities and polyphenol contents of sauces were not significantly different except for those of sauces with 1 g of angelica roots. Lastly, flavonoid contents of sauces significantly decreased with decreasing amount of angelica roots.

• Journal of Ginseng Research
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• 제21권3호
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• pp.187-195
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• 1997

The Chinese ginseng roots were collected at twelve places of Jilin Province and two places of Liaoning Province in China and their appearances were compared with those of the Korean ginseng roots. The color of the most of the Chinese red ginseng was brown or dark brown and the color of many of the Chinese dried ginseng was pale yellow and the root-age of the most of the Chinese red ginseng as well as the Chinese dried ginseng was evaluated five or six year regardless of the collection places, so it cannot be easily concluded that the color and the root-age of the Chinese ginseng roots are different from those of the Korean ginseng roots. However the rhizomes and the lateral roots of the Chinese ginseng roots were poorly developed and many of them did not have either rhizome or lateral roots. Moreover the rhizomes of the Chinese red ginseng as well as the Chinese dried ginseng were much more easily removed than those of the Korean red ginseng and the Korean white ginseng. Therefore it is thought that the development status of the rhizome and the lateral roots of the Chinese ginseng roots are quite different from those of the Korean ginseng roots.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.151-162
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• 2001

산삼은 고유의 생약으로 민간 또는 한방에서 효능을 인정받아 왔으나 산삼의 희귀성으로 인하여 산삼에 대한 연구가 활발하지 못하였다. 따라서 본 실험은 식물 조직 배양 기술을 이용하여 산삼 부정근을 대량으로 배양하고 추출하여 화장품 원료로서의 적용 가능성을 연구하였다.

• Plant Biotechnology Reports
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• 제5권3호
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• pp.255-263
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• 2011

Codonopsis lanceolata (Campanulaceae) has been used in traditional medicines, as its roots contain several kinds of triterpenoid saponin with high medicinal values. In this work, we induced transgenic hairy roots of C. lanceolata and analyzed triterpenoid saponins from the hairy roots and hairy root-derived transgenic plants. Hairy roots were obtained from leaf explants by the transformation of Agrobacterium rhizogenes R1000. Transgenic hairy root lines were confirmed by the transcriptional activities of rolA, B, C, and D genes by RT-PCR. Transgenic root lines actively proliferated on hormone-free medium but not in nontransformed roots. Hairy roots contained richer triterpenoids (lancemaside A, foetidissimoside A, and aster saponin Hb) than nontransformed roots. Transgenic plants were regenerated from the hairy roots via somatic embryogenesis. They showed phenotypic alterations such as shortened shoots and an increased number of axillary buds and adventitious roots. The transgenic plants also contained higher triterpenoid levels than wild-type plants. These results suggest that hairy roots and transgenic plants of C. lanceolata could be used as medicinal materials for the production of triterpene saponins.