• Journal of Plant Biotechnology
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• v.44 no.3
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• pp.320-324
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• 2017

Development of modern agricultural machinery and accompanying agricultural development cause soil compaction and reduce growth by stressing roots. Kalanchoe pinnata was used to investigate the impact of stress on rooting and changes in plant growth and reproduction. K. pinnata forms somatic embryos capable of asexual reproduction at the edge of leaves. Impact of root pressurization of K. pinnata on somatic embryogenesis and organ differentiation according to external stress factors was investigated by using a high concentration of agar and this phenomenon was studied histologically. Agar concentration in culture media ranged from 0.5%-1.5% to induce a compression effect on roots. The stem and leaf of K. pinnata were subjected to a microtechnique process to study changes in tissue. In vivo, K. pinnata produced 2nd and 3rd plantlets at edges of leaves from lack of water and excessive lighting conditions. In in vitro culture studies, the lower the concentration of agar, the higher the population and the higher the biomass, but plantlet did not occur in leaf bends. Conversely, as concentration of agar increased, increase in the number of individuals was low. Plantlet development occurred only in agar 1.5% medium. The difference in agar concentration was a stressor in the root of K. pinnata, and thus the pattern of asexual reproduction changed from the division method in root to a plantlet generation in leaf. This suggests root pressurization may act as stress and change in the plant reproduction pattern.

• Korean Journal of Medicinal Crop Science
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• v.5 no.1
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• pp.21-27
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• 1997

This experiments were conducted to determine the effect of thidiazuron on forming tuberlets and plant regeneration of Pinellia ternata T. by tissue culture. The addition of $5\;{\mu}M$ TDZ to the medium had better regeneration than that of any other treatments of NAA and TDZ. At the combination treatments of NAA and TDZ, as the level of thidiazuron increased, the rate of shoot regeneration was incresed while the increment of NAA concentration inhibited the rate of shoot regeneration. The supplement of $5\;{\mu}M$ thidiazuron produced the best number of micro-tubers per explant and the number of micro-tuber formed was 25 in MS medium and 29 in MG medium on 30 day culture, respectively. Microtuber formation was the best on MG medium with 1.0 mg/l NAA and $5\;{\mu}M$ thidiazuron. MG medium was superior to MS and B5 medium for the growth of tuberlets. Half strength of MS medium with NAA 2 mg/l was the most effective for root formation. Rooting ability on nursery soil of plantlets produced in in vjtro was good as a 80% after 3 weeks.

• Korean Journal of Medicinal Crop Science
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• v.5 no.1
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• pp.14-20
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• 1997

To increase the efficiency of the callus induction in anther culture of Schizandra chinensis, the effects of culture stage, low temperature pretreatment, growth regulators, sucrose and gelling agents were tested on Murashige and Skoog's medium. And the effects of ABA and $AgNO_3$ on organogenesis were investigated. The most suitable stage for anther culture was at the middle to late uninucleate stage, of which the flower size was $6.2{\pm}0.6{\times}4.0{\pm}0.4mm(Length{\times}width)$, and the frequency of callus induction was 13.3%. The effect of low-temperature pretreatment on callus induction was highest as 12.5% in the trearment for 8 days at $4^{\circ}C$. The combination of 1.0 mg/L 2, 4-D and 0.25 mg/L kinetin for callus induction was most effective as 8.3% among various media. The frequency of callus induction was excellent as 10.8% in 4% sucrose. Effect of gelling agents on callus induction was highest as 9.0% on 0.6% Gelrite medium. The prevention of callus browning was effective on the media supplemented with ABA and $AgNO_3$ and rooting was promoted on medium supplemented with 5 mg/L ABA. But shoot was not developed.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.33 no.2
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• pp.146-156
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• 1988

This study was carried out to investigate the change of endosperm substance and seedling growth in rice seedlings for machine transplanting with use of two rice varieties, which Samgangbyeo (Indica${\times}$Japonica) and Dogjinbyeo (Japonica) were sown on May 8 with five levels of seeding density. 1. The total dry matter weight, leaf area, length of the longest root, number and length of new roots were negatively correlated with seeding density, and the plant height had the similar tendency to them from 20 days after sowing(DAS). 2. The rooting ability was negatively correlated with seeding density and decreased from 35 DAS in Dongjinbyeo. 3. The endosperm substance were exhausted at 25 to 30 DAS in Samgangbyeo and at 25 DAS in Dongjinbyeo. 4. The leaf area was more useful than the ratio of plant height to dry matter weight for judging the constitution of seedlings. 5. The reducing sugar contents in the endosperm were the greatest among parts of rice seedlings followed by leaf and root. The contents in leaf, root and endosperm reached maximal at 10 DAS, and varied with plant parts, seedling growth and varieties in different levels of seeding density. 6. The protein contents in leaf and root of rice seedlings became decreased with seedling growth., and were minimal values at 15 DAS. The content in endosperm was decreased soon after seeding, and minimal at 4 DAS with showing some fluctuation of protein content after then.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.37 no.2
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• pp.198-208
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• 1992

Recently, a nursery mat made from rock wool has realized transplanting of the younger seedlings with the ordinary transplanting machines for Chibyo and Chubyo(3 and 4～5 leaved seedling, respectively). The seedlings defined as the 'Nyubyo' or 'Nursling seedlings' became possible to achieve economic profits from the reduction in both working time and costs. It being widely noticed as a strategy to solve the difficulties in current rice cultivation. The nursling seedlings are 1.4 to 2.5 leaves and height at 4.5 to 7cm, grown 4 to 7 days after seeding. They maintain still up to 50 to 80% of their nutrients in the endosperm, and can grow by using only their own nutrients for a certain period of time after transplanting. Nursling seedlings take 2 days in the nursery chamber at 32$^{\circ}C$ after seeding, and 2 days in the greening house at $25^{\circ}C$. This is only 4 days, all together, to make the nursling seedlings of 1.5 leaves which are ready for transplanting. Watering is only needed once at the sowing time. It only takes 1 or 2 waterings even to raise a seedlings for a period of 7 days. The number of nursery boxes can be reduced because it is possible to sow more densely(220 to 240g per box), thus it only needs seedlings of 15 to 16 boxes per 10 a which leads to a reduction in facilities and space needed. Temperature during the nursery period can be artificially adjusted more precisely which may lead to the prevention of temperature stress. The nursling seedlings can root rapid by because the crown roots from the coleoptile node begin to emerge immediately after transplanting. They show strong resistance to low temperature (12$^{\circ}C$) and deep-planting. There is no danger in the rooting of the seedlings even if half of their height is buried into the soil. Moreover, it can root at a rate of up to 65 to 80% even if the full height of the seedlings is buried. They show also strong resistance to submergence (10～15cm). The nursling seedlings tend to grow by producing tillers from lower nodes. It is therefore, necessary to control to keep the proper numbers of tillers per unit area. They have no fear in the delay of heading and their yield components can be so well balanced that the same level of yield was achieved with the nursling seedlings compared to that with Chibyo. It was further suggested that if the surplus tillers can be avoided by such cultivation practices, the number of grain per panicle can be kept greater and higher yield can be realized. Practical experiments with the nursling seedlings conducted in 1989 and 1990 by farmers in various areas showed exciting results. The nursling seedlings will become widely spread, or at least occupy an important position in Japanese and also in Korean rice cultivation techniques.tivation techniques.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.62 no.3
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• pp.259-274
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• 2017

Platycodon grandiflorum (Bell flower) is an important plant that has traditionally been used as herbal medicine for the treatment of cough, phlegm, sore throats, lung abscesses, chest pains, dysuria, and dysentery. The present study was initiated to investigate the feasibility of inducing shoot and root organogenesis in cultured explants of P. grandiflorum in a range of culture media and through use of various plant growth regulators (PGRs). The plantlets (Stem containing one node) were isolated and cultured on different concentrations of Murashige and Skoog (MS) medium supplemented with PGRs. We found that proliferation and elongation of shoots and roots could be achieved on 1/4 MS for P. grandiflorum with wild and green petals and on 1/8 MS for P. grandiflorum with double petals. The highest levels of development and elongation of adventitious shoots and roots were observed when petal explants were cultured on 1/4 MS (pH 3.8) supplemented with 5% sucrose. Increasing the agar concentration reduced shoot growth and rooting potential; nevertheless, the highest number of shoots and roots was observed on 0.6% agar. In the case of growth regulators, 1/4 MS supplemented with $1mg\;L^{-1}$ 6-benzylaminopurine (BA) was found to be best for shooting, although higher concentrations of BA tended to reduce shoot and root elongation. The highest number of shoots was achieved on $0.5mg{\cdot}L^{-1}$ thidiazuron (TDZ) from double petal explants grown on 1/8 MS. However, root and shoot elongation were found to decrease when TDZ concentrations were increased. Low concentrations of kinetin, naphthalene acetic acid, indole acetic acid, and 3-indole butyric acid induced shoot and root proliferation and elongation. Taken together, our study showed that low concentrations of PGRs induced the greatest root formation and elongation, showing that the optimal concentration of PGRs for shoot proliferation was species-dependent.

• Ecology and Resilient Infrastructure
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• v.6 no.4
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• pp.295-303
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• 2019

This study investigated the effects of various cultivation pots on Betula platyphylla var. japonica seedlings in order to select suitable pots for the production of healthy seedlings for the ecological revegetation. We used six types of the pots natural pot, natural pot with inside 5 mm width root turning bump, natural pot with inside 10 mm width root turning bump, negative-phototropism pot, negative-phototropism pot with inside 5 mm width root turning bump, and negative-phototropism pot with inside 10 mm width root turning bump. As greenhouse cultivation provided a high level of control of irrigation and temperature, the seedlings did not show any significant differences in plant height, number of branches, and diameter at root collar, but showed a significant difference in root growth among the different types of pots. The root growth was best in the negative-phototropism pot. In the negative-phototropism, the roots grew in a vertical shape, while they grew abnormally in a spiral shape in the natural pots. In outdoor cultivation, the growth of seedlings showed significant differences according to container types. The seedlings grown in specially manufactured negative-phototropism pots were most excellent in all of the height, number of branches, diameter at root collar and root growth. As for the seedlings grown in the negative-phototropism pots, their roots grew vertically and thus their rooting was well established after they were transplanted outdoor. A size of less than 5 mm ridge installed in the negative-phototropism was found to be appropriate for the seedling growth. These results showed that the negative-phototropism pot would be best suitable for the growth of Betula platyphylla var. japonica seedlings and for the production of seedling for ecological revegetation.

• Horticultural Science & Technology
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• v.16 no.4
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• pp.520-524
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• 1998

This study was carried out to investigate the effect of thidiazuron(TDZ) on callus and shoot primordia formation, to determine the most optimum multiple shoot induction medium, and to obtain the plantlets on solid medium via shoot organogenesis. TDZ 0.01 mg/L in MS medium was most effective on callus formation, and BA 0.1 mg/L was most effective on shoot growth, while TDZ 0.01 mg/L was most effective on callus formation. TDZ 0.001 mg/L was most effective in shoot primordia formation. Shoot tips were cultured with TDZ 0.01 mg/L for 8 weeks and induced callus was transferred to regeneration medium containing TDZ 0.001 mg/L. After 4 weeks induced shoot primordia were resubcultured at growth regulator-free medium for 4 weeks. The induced multiple shoots rooted more efficiently at NAA 1.0, 5.0 mg/L, or IBA 5.0 mg/L.

• Horticultural Science & Technology
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• v.17 no.2
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• pp.111-114
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• 1999

Experiments were conducted to select the optimum cultivars which are adaptable to South Korea through estimating the differences in daughter plant production and growth response among seven strawberry cultivars. In the vegetative stage, Nyoho, Akihime and Suhong produced more daughter plants than other cultivars whereas Akaneko and Tochinomine, which developed less runners, showed better condition in runner vigorness. The quality of daughter plants derived from forcing cultivars was uniform in general. Even though there was no significant differences in rooting among cultivars, runners from Akaneko and Tochinomine developed more roots and fresh weight of roots than other cultivars. In the forcing cultivars, Akihime showed the most excellent plant vigorness, yield and highest sugar content of fruits and also was 5 days faster than Nyoho in flowering date. However, the fruit tended to be softened. In the semi-forcing cultivars, Tochinomine was excellent in vigorness, firmness, sugar content of fruits and the average of fruit weight but low in flower numbers per cluster and late in the emergency of second flower cluster. Although Akaneko showed highest in sugar/acid ratio and rich in flavour, lighting treatment was recommended to this cultivar for early harvest because of softness of fruits.

• Magazine of the Korean Society of Agricultural Engineers
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• v.25 no.2
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• pp.35-41
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• 1983

This research is mainly to deal with the effects of submergence treatment on the grain yields of two rice plants, local variety, "Akibare" and Tongil line variety, "Milyang 23". The results obtained are summarized as follows. 1. According to the rice products of each plot the grain yield index was smallest in the plot treated at the early heading stage. The index of the next order became smaller in order of late flowering stage, late reduction division stage, milk ripe stage and dough ripe stage etc. The submerged stage at which the damages were smallest was tillering stage. Under the condition of two thirds or one third submerged depth of plant height, few differences in the grain yield index between Milyang 23 and Akibare was found, but except rooting stage, the damages of milyang 23 by whole submergence during growing period were mostly greater than those of Akibare. Especially the grain yield index of early heading stage at which the damages by whole submergence was most serious showed 45 percentage for one day submergence, 31 percentage for 3 days and 0.7 percentage for 7 days in Akibare plots, and 26.7% percentage for one day submergence, 7.9 percentage for 3 days and none for 7 days in Milyang 23 plots. 2. All the factors such as submerged stage, submerged depth and submerged period in this experimental test were highly recognized significance. The factors of the submerged depth and duration influenced on greater damages than the others. According to the difference in grain yield between plots, the larger the submerged depth and duration were, the larger the significant difference appeared. And between the treated levels at other submerged stages except both early tillering stage and most active tillering stage, the significance in the differences in grain yield was recognized, while only the submergence at early heading stage showed the most serious damages. 3. The decreased rate of grain yield for one day submergence at early heading stage indicated that in case of whole submergence of plant height it was 73 percentage in Milyang 23 plot and 55 percentage in Akibare plot, and in the event of two thirds and one third submergences of it, 20 percentage and 10 percentage in both Mulyang 23 and Akibare plots respectively. Therefore, the current criteria for planning project that restricted allowable submergence duration of more than 30cm submerged depth to 24 hours, should be amended not to exceed the submerged depth of 60cm when the duration of more than allowable submerged depth of 30cm will be limited to 24 hours, or within the limits of 12 hour submerged duration for locai variety and of less than 12 hour duration for Tong-illine variety as long as possible in case that submerged depth will be allowed to more than 60cm depth.ore than 60cm depth.