• Journal of Periodontal and Implant Science
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• v.26 no.3
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• pp.701-713
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• 1996

The purpose of this study was to evaluate the biocompatibility of the Nd:YAG lased root surface followed by root planing and/or tetracyline-HCI(T.C.-HCI) conditioning. $30,4mm{\times}4mm$ root segments were obtained from unerupted third molars and 21, periodontally involved root segments. The treatment groups were as follows : (1) healthy root cementum surface groups : 1) control(non-treated group), 2) lased only, 3) lased/root planed, and 4) lased/T.C.-HCI. (2) diseased root cementum surface groups : 1) control(root planed only), 2) lased/root planed, and 3) lased/root planed/T.C.-HCI. The specimens were treated with a Nd:YAG laser using a $320{\mu}m$ noncontact optic fiber handpiece with an energy setting of 1.5W($114.6J/cm^2$), 2.0W($152.9J/cm^2$), 5.0W($382J/cm^2$) for one minute. The fiber was held perpendicular to the petri dish(NUNC) 2cm apart in an attempt to expose the entire root segments equally. Human gingival fibroblasts were cultured from explants of normal interdental gingival tissue obtained during third morlar extraction. The attachment assay was performed with third-generation fibroblasts. The numbers of gingival fibroblasts attached to the root surface were counted on each specimen under the light microscope, and were statistically analyzed by the oneway ANOVA followed by Tukey's test in SPSS/PC+programs. The results were as follows : 1) In healthy root cementum surfaces, lased/root planed groups exhibited a significantly increased fibroblast attachment compared to controls, lased only, and lased/T.C.-HCI groups(p<0.05), 2) In diseased root cementum surfaces, laser treatment followed by root planing and/or T.C.HCl groups exhibited a increased tendency of fibroblast attachment compared to root planed only group. The results suggest that laser treatment followed by root planing and/or T.C.-HCl would appear necessary so as to render the root surface biocompatible.

• Korean Journal of Agricultural Science
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• v.38 no.2
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• pp.213-217
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• 2011

Agrobacterium rhizogenes, a gram-negative soil bacterium, is one of the most widely studied among them. A, rhizogenes can transfer T-DNA, excised from Ri (root inducing)-plasmids from the bacterial to the plant cell. It is the causal agent of 'hairy root' diseases in plants, and has been used for the production of hairy root cultures from a multitude of species. Five different strains of Agrobacterium rhizogenes differed in their ability to induce Scutellaria baicalensis hairy roots and also showed varying effects on the growth in hairy root cultures. A. rhizogenes R 1000 is the most effective strain for the induction (57.3%) and growth (11.9 g $L^{-1}$) in hairy root of Scutellaria baicalensis. Our results demonstrate that use of suitable strains of A. rhizogenes may allow study of the regulation of flavone biosynthesis in hairy root cultures of Scutellaria baicalensis.

• Journal of Ginseng Research
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• v.16 no.1
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• pp.31-36
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• 1992

The ultrastructural changes and differentiation mechanism of chromoplasts and leucoplasts from Proplastids in root tip cells of Panax ginseng seedlings were studied with transmission electron microscope. Initial cells have so many proplastids with a few osmiophilic droplets and a lot of mitochon dria at early stage of germination, therefore electron density of cytoplasm is generally higher than that of the other cells just like periblem, plerome and root cap. Proplastids are observed in the initial cells, but only leucoplasts appeared in the central root cap cells. Because root cap cells are derived ultimately from initial cells, the cell organelles in the root cap cells are directly related by those of initial cells. This result postulates that leucoplast is diferentiated from proplastid, and this is the same with other's concepts. On the contrary, the precise observations of chromoplast with crystalline inclusions in the peripheral root cap cells can conclude the direct pathway of chromoplast development from proplastid. Because of the differences of these result from those of other experiments, new scheme of plastid development, direct differentiation of chromoplast from proplastid, can be postulated. And this is the originality of this research on the differentiation of plastids.

• Restorative Dentistry and Endodontics
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• v.43 no.1
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• pp.10.1-10.8
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• 2018

Objectives: To examine the surface topography of intact WaveOne (WO; Dentsply Sirona Endodontics) and WaveOne Gold (WOG; Dentsply Sirona Endodontics) nickel-titanium rotary files and to evaluate the presence of alterations to the surface topography after root canal preparations of severely curved root canals in molar teeth. Materials and Methods: Forty-eight severely curved canals of extracted molar teeth were divided into 2 groups (n = 24/each group). In group 1, the canals were prepared using WO and in group 2, the canals were prepared using WOG files. After the preparation of 3 root canals, instruments were subjected to atomic force microscopy analysis. Average roughness and root mean square values were chosen to investigate the surface features of endodontic files. The data was analyzed using one-way analysis of variance and post hoc Tamhane's tests at 5% significant level. Results: The surface roughness values of WO and WOG files significantly changed after use in root canals (p < 0.05). The used WOG files exhibited higher surface roughness change when compared with the used WO files (p < 0.05). Conclusions: Using WO and WOG Primary files in 3 root canals affected the surface topography of the files. After being used in root canals, the WOG files showed a higher level of surface porosity value than the WO files.

• Journal of The Korean Society of Grassland and Forage Science
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• v.17 no.4
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• pp.345-350
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• 1997

Using modified Lincoln solution, hydroponic system was developed to compare simultaneously a range of pasture species in terms of relative growth rate(RGR), rate of roots to shoots, and relative root volume and root area. Modified Lincoln solution achieved optimum growth of nineteen forage species tested, and was proved to be a good method for studying plant root characteristics. RGR was measured sucesshlly showing reasonably consistant values over time. There was a much differences in rootkhoot ratio between species within groups as between groups. Root tissue density was lower in grasses than legume or herb group. Root area index was very high for grasses and herbs, and this index should be useful for evaluating the comparative root system of different species.

• Restorative Dentistry and Endodontics
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• v.38 no.3
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• pp.172-177
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• 2013

The mesiobuccal root of the maxillary molars is well known to pose a hindrance during endodontic therapy. Presented here is a case of a maxillary left second molar where three canals were located in its mesiobuccal root with the use of visual and diagnostic aids. Difficulties encountered during the process of unveiling the tooth's internal anatomy were discussed. The dilemmas encountered pertained to the root canal configuration, the nomenclature of the extra canals, and the justification for the presence of a third canal. The root canal configuration of 3-2-1 was confirmed for the mesiobuccal root using information gained from clinical, radiographic, and multidetector computed tomography (MDCT) scan findings. This case demonstrates the need for efforts to locate extra canals in the mesiobuccal root of the maxillary molars as their internal anatomy remains a mystery.

• Restorative Dentistry and Endodontics
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• v.49 no.2
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• pp.16.1-16.10
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• 2024

Objectives: This cross-sectional study evaluated the prevalence of apical periodontitis (AP) and the technical quality of root canal fillings in an adult Kuwaiti subpopulation using cone-beam computed tomography (CBCT) images. Materials and Methods: Two experienced examiners analyzed 250 CBCT images obtained from Kuwaiti patients aged 15-65 years who attended government dental specialist clinics between January 2019 and September 2020. The assessment followed the radiographic scoring criteria proposed by De Moor for periapical status and the technical quality of root canal filling. Chi-square and Fisher's exact tests were used for statistical analysis, with significance level set at p < 0.05. Results: Among the 2,762 examined teeth, 191 (6.91%) exhibited radiographic signs of AP, and 176 (6.37%) had undergone root canal filling. AP prevalence in root canal-treated teeth was 32.38%, with a significant difference between males and females. Most of the endodontically treated teeth exhibited adequate root canal filling (71.5%). Conclusions: The study demonstrated a comparable prevalence of AP and satisfactory execution of root canal treatment compared to similar studies in different countries.

• Korean Journal of Plant Resources
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• v.12 no.1
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• pp.1-9
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• 1999

The experiments were carried out to determine the optimum conditions for the induction of hairy roots in ginseng(Panax ginseng C.A. Meyer) by Agrobacterium spp. We were examined the antibiotics resistance of Agrobacterium spp and various ginseng parts, and the media for induction of hairy roots. The optimum concentration of NaOCl for sterilization of ginseng root segments without tissue damage with reduce of contamination was 7% NaOCl for 15-20 min and 9% NaOCl for 5 min, respectively. The more ginseng ages, the more contamination of ginseng root segment by sterilized in 7% NaOCl for 20 min, and especially in ginseng root segments with epidermis in six-year old roots. The growth of Agrobacterium spp were inhibited, but ginseng root segments was death in 30mg/L tetracycline. In 500mg/L cefotaxime or 500mg/L carbenicillin, the growth of Agrobacterium sup were inhibited, and root segments was grown normally. The optimum conditions for induction of hairy roots were using the root segments of three-year old ginseng cultured in 1/2MS medium supplemented with 500mg/L cefotaxime, and inoculation of Agrobacterium to root segments were better co-culture than smear method. After 2 weeks co-culture, the callus induced in cambium of root segments cultured in 1/2MS solid medium with 500mg/L cefotaxime. And then after 2 weeks, ginseng hairy roots were induced in callus of root segments. PCR analysis of rot C gene fragment confirmed that hairy roots were transgenic tissues.

• Geotechnical Engineering
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• v.13 no.5
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• pp.101-124
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• 1997

The root pile system is insitu soil reinforcement technique that uses a series of reticulately installed micropiles. In terms of mechanical improvement by means of grouted reinform ming elements, the root pile system is similar to the soil nailing system. The main difference between root piles and soil nailing are due to the fact that the reinforcing bars in root piles are normally grouted under high pressure and that the alignments of the reinforcing members differ. Recently, the root pile system has been broadly used to stabilize slopes and retain excavations. The accurate design of the root pile system is, however, a very difficult tass owing to geometric variety and statical indetermination, and to the difficulty in the soilfiles interaction analysis. As a result, moat of the current design methods have been heavily dependent on the experiences and approximate approach. This paper proposes a quasi-three dimensional method of analysis for the root pile system applied to the stabilization of slopes. The proposed methods of analysis include i) a technique to estimate the change in borehole radium as a function of the grout pressure as well as a function of the time when the grout pressure is applied, ii) a technique to evaluate quasi -three dimensional limit-equilibrium stability for sliding, iii) a technique to predict the stability with respect to plastic deformation of the soil between adjacent root piles, and iv) a quasi -three dimensional finite element technique to compute stresses and dis placements of the root pile structure barred on the generalized plane strain condition and composite unit cell concept talon형 with considerations of the group effect and knot effect. By using the proposed technique to estimate the change in borehole radius as a function of the grout pressure as well as a function of the time, the estimations are made and compar ed with the Kleyner 8l Krizek's experimental test results. Also by using the proposed quasi-three dimensional analytical method, analyses have been performed with the aim of pointing out the effects of various factors on the interaction behaviors of the root pile system.

• Proceedings of the KACD Conference
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• 2001.05a
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• pp.259-259
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• 2001

;A new root canal instrument and instrumentation technique: a preliminary report. Cleaning and shaping the root canal system has been and continues to be a challenge for even the most experienced endodontist. Curved. narrow canals. in particular. cause difficulties for the beginner as well as the specialist. A new instrument designed to incorporate new concepts was developed to ameliorate the problems in cleaning and shaping root canal systems. This new instrument has been given the name of SW (Senia and Wildey). The new SW instrument uses controlled right and left rotational forces. This motion was used to clean and shape simulated root canals in plastic blocks and root canals in extracted teeth. Instrumentation appeared to be easier. faster. and more precise than with conventional instruments. especially in curved canals where there was remarkable reduction of canal transportation. A mechanical version of the SW instrument was also developed. It was used to flare the coronal portion of the root canal system. Wildey WL. Senia ES., Oral Surg Oral Med Oral Pathol1989 Feb:67(2):198-207 Another look at root canal instrumentation. Several aspects of root canal instrumentation need additional research. Various factors must be considered in an analysis of instrumentation of the root canal system: the dentin that is cut: the technique used to cut it: the design of the instruments: the material and manufacturing process used to make the instruments: the irrigant used during the procedure: and the anatomic configuration of the root canal system. An analysis of these factors clearly indicates that existing root canal instruments and techniques are less than ideal and. in fact. do not accomplish what is expected of them. Root canals must be properly. but. at the same time. destructive and unnecessary removal of dentin should be kept to a minimum. The Flex-R and Canal Master instruments were developed to address some of the shortcomings of existing instruments and techniques. More scientifically based research is needed to fully evaluate these new instruments and techniques and to develop future instruments. Wildey WL; Senia ES. Montgomery S., Oral Surg Oral Med Oral Pathol1992 Oct:74(4):499-50799-507