• KOREAN JOURNAL OF CROP SCIENCE
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• v.67 no.1
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• pp.67-75
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• 2022

Roots play important roles in water and nutrient uptake and in response to various environmental stresses. Investigating diversification of cultivars through root phenotyping is important for crop improvement in adzuki beans. Therefore, we analyzed the morphological and architectural root traits of 22 adzuki bean cultivars using 2-dimensional (2D) root imaging. Plants were grown in plastic tubes [6 cm (diameter) × 40 cm (height)] in a greenhouse from July 25^th to August 28^th. When the plants reached the 2^nd or 3^rd trifoliate leaf stage, the roots were removed and washed with tap water to remove soil particles. Clean root samples were scanned, and the scanned images were analyzed using the WinRHIZO Pro software. The cultivars were analyzed based on six root phenotypes [total root length (TRL), surface area (SA), average diameter (AD), and number of tips (NT) were included as root morphological traits (RMT); and link average length (LAL) and link average diameter (LAD) were included as root architectural traits (RAT)]. According to the analysis of variance (ANOVA), a significant difference was observed between the cultivars for all root morphological traits. Distribution analysis demonstrated that all root traits except LAL followed a normally distributed curve. In the correlation test, the most important morphological trait, TRL, showed a strong positive correlation with SA (r = 0.97^***) and NT (r = 0.94^***). In comparison, between RMT and RAT, TRL showed a significantly negative correlation with LAL (r = -0.50^***); however, TRL did not show a correlation with LAD. Based on RMT and RAT, we identified the cultivars that ranked 5% from the top and bottom. In particular, the cultivar "IT 236657" showed the highest TRL, SA, and NT, while the cultivar "IT 236169" showed the lowest values for TRL, SA, and NT. In addition, the coefficient of variance for the six tested root traits ranged from (14.26-40%) which suggested statistical variability in root phenotypes among the 22 adzuki bean varieties. Thus, this study will help to select target root traits for the adzuki bean breeding program in the future, generating climate-resilient adzuki beans, especially for drought stress, and may be useful for developing biotic and abiotic stress-tolerant cultivars based on better root trait attributes.

• Journal of Korean Society of Forest Science
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• v.96 no.2
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• pp.178-182
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• 2007

Rauvolfia serpentina (L.) Benth. ex Kurz is a medicinal plant and an endangered tropical rainforest plant species. Since the field cultivation that aims to fulfill the industrial needs is never accomplished, tissue culture appears to be the most feasible way to improve the quality and quantity of R. serpentina. This experiment used two kinds of explants (roots and shoots) to induce optimal root formation in different combinations of auxin and photoperiod. Each explants exhibited different responses on given treatments. Differentiated root could be produced from explants cultured in IBA 20 mg/L with and without light. The highest number of roots, root length and root weight induced from shoot explants were effective on MS medium containing IBA 20 mg/L and incubated under dark condition, while highest total weight (callus and root) from root explants cultured on MS medium supplemented 10 mg/L IBA and 10 mg/L NAA and incubated under day length (11/13 hr). The root induced from shoot explants produced the highest major alkaloid content. The highest content of ajmaline (2.17 ppm fresh weight) and reserpine (1.30 ppm fresh weight) were observed in shoot explants cultured in MS medium containing combination of IBA 10 mg/L and NAA 10 mg/L and incubated under dark condition, yohimbine (1.47 ppm fresh weight) was in the shoot explants cultured in MS medium containing NAA 20 mg/L and incubated under day length, while serpentine was absent.

• Restorative Dentistry and Endodontics
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• v.40 no.1
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• pp.44-49
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• 2015

Objectives: This study compared the ability of several techniques to remove calcium hydroxide (CH) from the root canal and determined the influence of CH residues on the accuracy of the electronic apex locator. Materials and Methods: Root canals of 90 human maxillary lateral incisors with confirmed true working length (TWL) were prepared and filled with CH. The teeth were randomly assigned to one of the experimental groups according to the CH removal technique (n = 14): 0.9% saline; 0.9% saline + master apical file (MAF); 17% ethylenediamine tetraacetic acid (EDTA); 17% EDTA + MAF; 5.25% sodium hypochlorite (NaOCl); 5.25% NaOCl + MAF. Six teeth were used as negative control. After CH removal, the electronic working length was measured using Root-ZX (Morita Corp.) and compared with TWL to evaluate Root-ZX accuracy. All specimens were sectioned longitudinally, and the area of remaining CH (CH) and total canal area were measured using imaging software. Results: The EDTA + MAF and NaOCl + MAF groups showed better CH removal than other groups (p < 0.05). Root-ZX reliability to prevent overestimated working length to be > 85% within a tolerance of ${\pm}1.0mm$ (p < 0.05). There was strong negative correlation between amount of CH residues and EAL accuracy (r = -0.800 for ${\pm}0.5mm$; r = -0.940 for ${\pm}1.0mm$). Conclusions: The mechanical instrumentation improves the CH removal of irrigation solutions although none of the techniques removed the dressing completely. Residues of CH medication in root canals affected the accuracy of Root-ZX adversely.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2001.11b
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• pp.49-61
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• 2001

This study was carried out to determine the effect of various fertilizer levels, sowing time and planting density on the growth and yield of Scuteliaria baicalensis GEORGE cultivated after the barley in the southern coastal areas of Korea under the non- mulching condition by direct sowing culture. The flowering date of medium dressing plot(N : P$_2$O$\sub$5/ : K$_2$O=9 : 13.5 : 9kg/10a) and heavy dression plot(N : P$_2$O$\sub$5/, : K$_2$O=12 :18 : 12kg/10a) were July 23. The flowering date of the medium and the heavy dressing plot was delayed by 3days compared with that of non-fertilizing plot. The growth characteristics such as stem length, diameter of main stem, number of branch per plant, main root length, main root length, main root thickness and dry weight of stem leaves were more increased at medium dressing plot than that of other fertilizer levels, The root dry weight of in Scutellaria baicalensis GEORGE cultivated after barley was hlghest at the fertilizing plot of N : P$_2$O$\sub$5/ : K$_2$O=9 : 13.5 : 9kg/10a. The dried-root yield was 178kg in medium dressing plot, 167kg in standard dressing pot, and 126kg in non-dressing plot, The dried-root yield of medium dressing plot was 7% and 41 cie higher than that of standard dressing pot and non-dressing control plot, respectively. Emergence and flowering dates in the sowing time of June 1 were earlier than those of the other sowing times. In the sowing time of June 1, length and diameter of main stem, number of node per main stem, number of branch per plant and dry weight of stem leaves were greater than those of sowing times of June 10 and June 20. Yield components such as main stem length and diameter, main stem numbers, branches per plant, dry weight of stem leaves, main root length and thickness, number of large root and fine root per plant, and dry weight of root were the highest at the sewing time of June 1 as the yield of 71.3kg/10a. Optimum sowing time of Scuteilaria baicaiensis GEORGE cultivated after barley was June 1 in southern areas of Korea. Stem length was long in dense planting of 20${\times}$10cm and short in spacious planting of 30${\times}$10cm and 40${\times}$10cm by direct sowing cultivated after barley. Stem diameter was thick in spacious planting of 30${\times}$10cm and 40${\times}$10cm and was thin in dense planting of 20${\times}$10cm by direct sowing cultivated after barley. Length and dry weight of root per plant were decreased in dense planting of 20${\times}$10cm and were increased in spacious planting of 30${\times}$10cm and 40${\times}$10cm by direct sowing cultivated after barley. Yield of dry root was highest in optimum planting density(30${\times}$10cm 33 plants/㎡) by direct sowing cultivated after barley. The correlation coefficient between number of planting plant and stem length showed highly positive correlation. These characters of stem diameter, number of branches, main root length and yield of dry root mentioned above showed negative correlations with planting plants.

• Journal of Korean Academy of Oral and Maxillofacial Radiology
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• v.2 no.1
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• pp.53-57
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• 1972

The author measured the length of crown, root and tooth on the films which was taken by intraoral bisecting technic with mesh plate on the films. The films were taken from the dry skulls, dentiform and patients who had to be removed their upper incisors and the other patients who adimitted for dental care. From this serial experiment the results was made as follows: 1. By using the film and mesh plate in the oral cavity, the real tooth length can be measured easily on the film surfaces. 2. It can be avoided the film distortion in the oral cavity when taking the film using the mesh plate and film together. 3. When measuring the film, length of crown was elongated and length of root was shortened. 4. When using the well-trained bisecting technic, the real tooth length can be measured directly on the intraoral film.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.26 no.5
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• pp.47-56
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• 2023

The Quercus variabilis and Quercus dentata, which are said to be relatively drought tolerant among the important genus Quercus that represent deciduous broad-leaved forests in Korea. These two species are widely distributed worldwide in Korea, Japan and China (northern, central, western and eastern subtropical regions). This study compared the ecological niche breadth and overlap according to growth response in 4 soil moisture gradients for the two species and tried to reveal degree of competition and ecological niche characteristics. The ecological niche breadth was 0.977±0.020 for Q. variabilis and 0.979±0.014 for Q. dentata, the latter being slightly wider. And they were similar in 5 traits (stem length, leaf lamina length, leaf width length, stem weight, leaf petiole weight), Q. variabilis was more dominant in 4 traits (leaves number, stem diameter, leaf area, leaf petiole length), and Q. dentata was more dominant in 7 traits (root length, shoot length, plant weight, root weight, shoot weight, leaf weight, leaf petiole weight). The ecological niche overlap for soil moisture between the two species overlapped most in plant structure-related traits and least in photosynthetic organ-related traits such as petiole length. As a result of principal component analysis, degree of competition between the two species for soil moisture was more severe when the soil moisture condition was low than high. Among the measured traits that affect the two-dimensional distribution, 8 traits (Leaves number, Shoot length, Stem length, Plant weight, Root weight, Shoot weight, Stem weight, Leaves weight) were correlated with the factor 1, and 2 traits (Leaf width length, Leaf petiole weight) were correlated with the factor 2 (r>0.5). These results show that the ecological response of the two species to soil moisture is not a few traits involved, but several traits are involved simultaneously.

• Journal of Bio-Environment Control
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• v.17 no.1
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• pp.14-19
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• 2008

The experiment was investigated the effects of lower root zone temperature on shoot and root growth of 'Akihime' strawberries in aeroponics in cold season. Root growth was decreased with decrease of root zone temperature, especially in lateral roots. Elongation of main roots was highest in $18^{\circ}C$ of root zone temperature. Number of lateral roots and length of lateral roots were lowest in $8^{\circ}C$ of root zone temperature. Lower root zone temperature resulted significantly in decrease of fresh weight of root and shoot and leaf area of strawberry. But there were no significant statistical differences in shoot fresh weight and leaf area in $8^{\circ}C$ and $13^{\circ}C$ of root zone temperature. Leaf length, leaf width and number of leaves of strawberry were decreased in lower root zone temperature. The results of this experiment will be utilized in the winter season cultivation for strawberry in hydroponics.

• Imaging Science in Dentistry
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• v.36 no.3
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• pp.137-143
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• 2006

Purpose : To evaluate location, distribution, diameter, and length of the nutrient canals on mandibular anterior region using a cone beam computed tomography (CBCT). Materials and Methods : Mandibular CBCT was performed on 33 adults (18 males and 15 females) with no history of systemic disease, and any other dental surgery history Location, distribution, diameter, and length of the nutrient canals on mandibular anterior region were radiographically evaluated. A statistical comparison was done by SPSS. Results : In the location and distribution of nutrient canals, they were found in 6.8% at labial portion above root apex, in 93.28% at lingual portion above root apex in 46.2% at labial portion below root apex, and in 53.6% at lingual portion below root apex. Nutrient canals at lingual portion above root apex were most frequently observed between central and lateral incisors, and those at labial and lingual portion below root apex were most frequently observed between central incisors. The mean diameters of nutrient canals were 0.54 mm at labial portion above root apex, 0.61 mm at lingual portion above root apex, 0.66 mm at labial portion below root apex, and 0.76 mm at lingual portion below root apex. The mean lengths of nutrient canals were 2.63 mm at labial portion above root apex, 3.74 mm at lingual portion above root apex, 4.51 mm at labial portion below root apex, and 6.77 mm at lingual portion below root apex. Conclusion : CBCT is useful device to evaluate the anatomical structure of nutrient canals on mandibular anterior region.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2000.05a
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• pp.38-44
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• 2000

To determine the optimum sowing date and nursery period in Alisma piantago in the southern area of Korea, Alisma plantago local cultivar was grown under three different sowing dates and nursery periods. The plant height, leaf width and leaf length at the area sown on the seedbed in June 30 and conducted the growing seedling in 30 days is 12.1cm, 2.5cm and 3.1cm respectively and it shows tess decrease than that of plant height, leaf width and leaf length with 12.3cm, 2.6cm and 3.2cm respectively which was sown on the seedbed in June 20 and carried out the growing seedling in 30 days, and it also shows less decrease than that of plant height, leaf width and leaf length with 15.2cm, 3.1cm and 5.2cm respectively at the area sown on the seedbed in July 10 and condected the growing seedling in the same date. The stem length, number of stems and diameter of root at the area sown on the seedbed in June 30 and conducted the growing seedling in 30 days is 31cm, 11 and 3.7cm respectively and it shows more increase than that of stem length, number of stem and diameter of and on the other hand shows tess decrease than that of stem length, number of stems and diameter of root with 30cm, 10cm, 35cm respectively which was sown on the seedbed in 30 days, and on the other hand shows less decrease than that of stem length, number of stems and diameter of root with 32cm,13cm,3.9cm respectively at the area sown on the seedbed in July 10 and conducted the growing seedling in the same date, The fresh yield of roots at the area sown on the seedbed in June 30 and conducted the growing seedling in 30 days is 431.4Kg/10a and it shows more increase of 26.9Kg than that of fresh yield of root with 410.59g110a which was sown on the seedbed in lune 20an4 carried out the growing seedling in 30 days, and it also shows more increase of 7Kg than that of fresh yield of root with 430.4Kg/10a at the area sown on the seedbed in July 10 and conducted the growing seedling in the same date. The dry yield of roots at the area on the seedbed in June 30 and conducted the growing seedling in 30 days is 351.9Kg110a which was sown on the seedbed in June 20 and carried out the growing seedling in 30 days, and it also shows more increase of 16.4Kg than that of dry yield of root with 335.4Kg110a at the area sown on the seedbed in July 10 and conducted the growing seedling in the same date. Therefore, the seedling period of proper seedbed for high yield bumper crop of Alisma plantago for exporting to Japan is June 30 and the number of days for seedling is 30 days.

• Restorative Dentistry and Endodontics
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• v.30 no.6
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• pp.477-485
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• 2005

The aim of this study was to compare the length between the mesio-buccal and mesio-lingual canal of the mandibular molars before and after early coronal flaring at the different measuring time using several electronic apex locators. Fifty mandibular molars with complete apical formation and patent foramens were selected. After establishing the initial working length of the buccal and lingual canal of the mesial root using a surgical microscope (Carl Zeiss Co Germany) at 25X with #15 K-fle tip just visible at the foramen, radiographs were taken for the working length. After measuring the length of mesio-buccal and mesio-lingual canal (control group), the electronic lengths were measured at different times using several electronic apex locators (experimental groups; I-Root ZX, II-Bingo, III-Propex, IV-Diagnostic). After early coronal flaring using the $K^3$ file, the additional electronic lengths were measured using the same manner The results were as follows: One canal has a correct working length for the mesial root of the mandibular molar, it can be used effectively for measuring the electronic working length of another canal when the files are superimposed or encountered at the apex. In addition, the accuracy of the electronic apex locators was increased as the measurement was accomplished after the early coronal flaring of the root canal and the measuring time was repeated.