• Title/Summary/Keyword: Role frame

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Gene Structure of Cotesia plutellae Bracovirus (CpBV)-$I_{k}B$ and Its Expression Pattern in the Parasitized Diamondback Moth, Plutella xylostella (프루텔고치벌 브라코바이러스(Cotesia plutellae Bracovirus) 유래 $I_{k}B$ 유전자 구조와 피기생 배추좀나방(Plutella xylostella) 체내 발현 패턴)

  • Kim Yong-Gyun;Basio Neil A.;Ibrahim Ahmed M.A.;Bae Sung-Woo
    • Korean journal of applied entomology
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    • v.45 no.1 s.142
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    • pp.15-24
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    • 2006
  • Inhibitor kB (IkB)-like gene has been found in the genome of Cotesia plutellae bracovirus (CpBV), which is the obligatory symbiont of an endoparsitoid wasp, C. plutellae. The open reading frame of CpBV-IkB was 417 bp and encoded 138 amino acids. Four ankyrin repeat domains were found in CpBV-IkB, which shared high homology with other known polydnavirus IkBs. Considering a presumptive cellular IkB based on Drosophila Cactus, CpBV-IkB exhibited a truncated structure with deletion of signal-receiving domains, which suggested its irreversible inhibitory role in NFkB signal transduction pathway of the parasitized host in response to the wasp parasitization. CpBV-IkB was expressed only in the parasitized diamondback moth, Plutella flostella. Its expression was estimated by quantitative RT-PCR during parasitization period, showing a constitutive expression pattern from the first day of parasitization. An indirect functional analysis of CpBV-IkB was conducted and suggested a hypothesis of host antivirus inhibition.

Mating Disruption of Grapholita molesta by RNA Interference of a Fatty Acid Desaturase Expressed in Adult Abdomen (복숭아순나방 성충 복부에서 발현하는 불포화효소의 RNA 간섭과 교미교란)

  • Kim, Kyusoon;Jung, Chung Ryul;Yang, Chang Yeol;Kwon, Gimyeon;Kim, Yonggyun
    • Korean journal of applied entomology
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    • v.56 no.1
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    • pp.61-67
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    • 2017
  • Two major sex pheromone components (Z-8-dodecenyl acetate and E-8-dodecenyl acetate) are known in the peach fruit moth, Grapholita molesta. From a putative biosynthetic pathway of these sex pheromone components, delta 10 desaturase ($10{\Delta}$ DES) has been proposed to play a crucial role in synthesizing a species-specific stereoisomer of the double bond. However, its molecular identity was not known. This study determined a putative desaturase (Gm-comp1575) as a $10{\Delta}$ DES candidate from G. molesta transcriptome constructed from the sex pheromone gland. Its open reading frame encodes 370 amino acid sequence with a predicted molecular weight at 43.2 kDa and isoelectric point at 8.77. It was predicted to have four transmembrane domains and six glycosylation sites at N-terminal or cytosolic domains. A phylogenetic analysis with its predicted amino acid sequence indicated that Gm-comp1575 is closely related with known $10{\Delta}$ DES genes of other insects. Gm-comp1575 transcript was detected in female adults at sex pheromone gland and other abdominal tissues. RNA interference of Gm-comp1575 significantly reduced attractiveness of virgin females in apple orchard compared to control females. These results suggest that Gm-comp1575 is associated with sex pheromone biosynthesis of G. molesta.

Korean Strategies for Establishing New MRV Schemes Applicable to Post-2020 Regime (Post-2020의 MRV 체계 수립을 위한 우리나라 대응 방향)

  • Kim, Seungdo;Ryu, SeungYun;Jung, Jaehyuk;Yoo, Beom-Sik
    • Journal of Environmental Policy
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    • v.14 no.4
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    • pp.3-21
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    • 2015
  • This paper attempts to determine the most reasonable and agreeable option for new MRV framework in Post-2020 Climate Regime as well as to develop South Korea strategies to reflect our national circumstances and capacities appropriately. The options for MRV framework considered here are 1) Dual MRV and 2) Unified MRV. We reached the conclusion that the Unified MRV framework may be the most reasonable option in the 2015 agreement in line with the objectives of MRV. To this end, it is essential to develop effective stepwise strategies consisted of: 1) reaching a consensus internally on the Unified MRV framework, 2) developing detailed procedures and methodologies to make the major elements of the Unified MRV framework understandable and predictable, 3) delivering an overview and blueprint to make sure of the flexibility of the Unified MRV framework to reflect the Parties' capacities and national circumstances, 4) ensuring the financial and technical support schemes for developing countries to improve the capacity-buildings of MRV, and 5) developing a realistic road map to successfully implement the Unified MRV scheme within the required time frame. Korea, as a rational mediator between developed and developing countries, should play a leading role in reaching post-2020 agreement.

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China's Global Investment Policy and Precondition for China Money FDI in Korea (중국의 해외투자 정책과 중국자본 유치의 전제조건)

  • Park, Moon-Suh;Kim, Mea-Jung
    • International Commerce and Information Review
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    • v.14 no.1
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    • pp.171-195
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    • 2012
  • This paper is aimed to prepare some policy-measures which is helpful for China Money FDI in Korea by analysing FDI-related data and political strength and weakness between the two countries and studying fundamental preconditions required for Korea's China Money FDI strategies. As the result of research, key preconditions found out can be summarized as follows; First, because China-Korea economic relationship is largely insufficient in a complementary view as well as in a cooperative state level, Korea should remove the threats in advance that could lead two countries to unlimited competition, and then expand to a relationship of trust between China and Korea. Second, Korea, at least from the perspective of China, may not be an attractive investment destination. Therefore, it is necessary to take advantage of Korea's FTA-expansion-strategy opportunities such as Korea-US FTA which has entered into force recently. Third, because China always has a lot of alternative investment opportunities among world instead of Korea, so Korea should not overlook the fact that China has the bargaining power in large part related on the investment conditions in Korea, such as investment field, investment size, how to invest China Money to Korea, etc. Fourth, if Korea's FDI policy is trapped in the existing rules of the political frame, and moreover Korea can not have the role of rule breaker, it will be difficult to expect Korea's China Money FDI results compared to those efforts. Fifth, if Korea will execute China Money FDI strategies in the context of overestimating the China Power or China Money, it should be noted that Korea may have unexpected losses lead to a national by reason of outward and quantitative investment or bad investment.

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A Study for Relationship Between the Femininity and the Nursing Image of Nursing Students (일부 간호학생의 여성성과 간호상과의 상관관계)

  • 성현란
    • Journal of Korean Academy of Nursing
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    • v.8 no.2
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    • pp.39-49
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    • 1978
  • The present research set out to further validate the results that Stromborg (1976) obtained in which a correlation (r= .22, p < .001) was found between the femininity of woman nursing students and their image of nursing. In addition the experimenter set out to elucidate any possible differences in the image of nursing according to (a) religion, (b) the motivation for choosing nursing as well as, (c) the particular area in which the students hoped to work after graduating. The research was conducted by presenting questionnaire type scales including a femininity scale and a nursing image scale, to 301 graduating students attending 4 Three-year nursing schools located within Seoul from November 21st, 1978 to November 30th. The results: 1. There was clearly no relation between femininity and the image of nursing as such (r= .01, p > .43). But upon analyzing the results along five subdimensions of the nursing image variable it was discovered that : (a) if femininity is low, the nursing student tend to view the educational preparation for becoming a nurse in a more positive light (r= -.10, p < .05), and (b) if femininity is high, the students tend to perceive their social role with a positive frame of mind (r= .15, p < .005). 2. There was a significant difference found in the image of nursing as related to the religion of the nursing students, The means ranked as follows (high scores indicate a positive image of the nursing profession). Catholic > Protestant > no religion > Buddhist 3, The difference in the nursing image according to motivation for sellecting the profession emerged as quite significant (F=6.92, p < .001). The rank of the means when the subject group is divided along the dimension of motive is as follows (ranking begins with the most positive nursing image): (a) The profession itself seemed good; (b) after marriage one can do a social type of activity; (c) significant others’advice: (d) the profession matches their high school grade record; (e) it matches the economic situation of the student; (f)no particular reason for their choice: (g) it's easy to get a nursing job after graduation. 4. The particular area of nursing one intended to go into after graduation varied significantly in terms of the image of nursing (F=3.03, p < .005). The means when ranked starting with those having the most positive image of the profession: (a) nurses helping deliveries; (b) hospital nurses: (c) nursing education; (d) school nurses: (e) government nurse supervisors: (f)public health nurses: (g) (students not going into any profession); (h) (students not going into the nursing profession but into some other area).

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Power Shift and Media Empowerment (언론의 정치권력화 - 재벌 정책 보도의 정권별 비교 연구)

  • Kim, Dong-Yule
    • Korean journal of communication and information
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    • v.45
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    • pp.296-340
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    • 2009
  • The power of media has always been problematic in the countries of full press freedom. Originally, the media used to be an effective vehicle for communication within human beings. However, it exerts an overwhelming power toward human society. Through applying the well-known four dog models in terms of media function, this study attempts to examine how the press media in South Korea transformed themselves into another powerful independent organization or institution after regime shift in 1987. The whole editorials of four sampled newspapers were analyzed through frame analysis model. The ChosunIlbo, known as a conservative and pro-government paper, shows to take the role of supporting chaebol policies under Roh TaeWoo Administration. However, it criticizing sharply against the chaebol policies of Roh MooHyun Administration. The JoongangIlbo, known as a pro-chaebol paper, appears anti-government position through the entire four administrations in terms of chaebol policies. Particularly, it reveals hostile editorial coverage during the Roh MooHyun Administration. However, KyunghyangShinmun, currently known as a liberal paper, viewed somewhat complicated positions (see text in more detail) because of its ownership turbulence during the past twenty years. On the other hand, Hangyoreh, regarded as a progressive paper, keeps in supportive attitude consistently against the four sampled administrations as far as regulating each government policies for chaebols.

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Exploring How a High School Science Teacher's Understanding and Facilitation of Scientific Modeling Shifted through Participation in a Professional Learning Community (교사학습공동체에 참여한 한 고등학교 교사의 과학적 모델링에 대한 이해 및 수업 실행 변화 탐색 -프레임 분석을 중심으로-)

  • Shim, Soo-Yean
    • Journal of The Korean Association For Science Education
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    • v.40 no.1
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    • pp.29-40
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    • 2020
  • The purpose of this study is to explore how a high school science teacher (Teacher E) shifted her understanding and facilitation of scientific modeling through participation in a professional learning community (PLC) for over a year. Based on socially situated theory of learning, I focused on examining Teacher E's frames about scientific modeling from her social interactions. Teacher E participated in her school-based PLC over a year and collaborated with other science teachers, coaches, and researchers to improve science instruction. I qualitatively explored her participation in 6 full-day professional learning opportunities-studios-where the PLC members collectively planned, implemented, and debriefed modeling-based lessons. Especially, I focused on two Studios (Studio 2, 6) where Teacher E became the host teacher and implemented the lessons. I also examined her classroom teaching in those Studios. To understand how the PLC inquiry affected the shifts observed in Teacher E's understanding and practice, I explored how the inquiry evolved over the 6 Studios. Findings suggest that in Studio 2, Teacher E viewed students' role in scientific modeling as to fill out the worksheet with "correct" answers. Meanwhile, in Studio 6, she focused on helping students collaborate to construct explanatory models of phenomena using evidence. The PLC inquiry, focused on supporting students' construction of evidence-based explanations and collaboration in scientific modeling, seemed to promote the shifts observed in Teacher E's understanding and facilitation of scientific modeling. These findings can inform educational researchers and practitioners who aim to promote teachers' professional learning to support students' epistemic practices.

Molecular Cloning, Characterization and Functional Analysis of a 2C-methyl-D-erythritol 2, 4-cyclodiphosphate Synthase Gene from Ginkgo biloba

  • Gao, Shi;Lin, Juan;Liu, Xuefen;Deng, Zhongxiang;Li, Yingjun;Sun, Xiaofen;Tang, Kexuan
    • BMB Reports
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    • v.39 no.5
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    • pp.502-510
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    • 2006
  • 2C-methyl-D-erythritol 2, 4-cyclodiphosphate synthase (MECPS, EC: 4.6.1.12) is the fifth enzyme of the non-mevalonate terpenoid pathway for isopentenyl diphosphate biosynthesis and is involved in the methylerythritol phosphate (MEP) pathway for ginkgolide biosynthesis. The full-length mecps cDNA sequence (designated as Gbmecps) was cloned and characterized for the first time from gymnosperm plant species, Ginkgo biloba, using RACE (rapid amplification of cDNA ends) technique. The full-length cDNA of Gbmecps was 874 bp containing a 720 bp open reading frame (ORF) encoding a peptide of 239 amino acids with a calculated molecular mass of 26.03 kDa and an isoelectric point of 8.83. Comparative and bioinformatic analyses revealed that GbMECPS showed extensive homology with MECPSs from other species and contained conserved residues owned by the MECPS protein family. Phylogenetic analysis indicated that GbMECPS was more ancient than other plant MECPSs. Tissue expression pattern analysis indicated that GbMECPS expressed the highest in roots, followed by in leaves, and the lowest in seeds. The color complementation assay indicated that GbMECPS could accelerate the accumulation of $\beta$-carotene. The cloning, characterization and functional analysis of GbMECPS will be helpful to understand more about the role of MECPS involved in the ginkgolides biosynthesis at the molecular level.

Dental Practitioners Self Reported Performance of Tobacco Cessation Counseling Interventions: A Cross Sectional Study

  • Parakh, Abhinav;Megalamanegowdru, Jayachandra;Agrawal, Rohit;Hathiwala, Siddhi;Chandrakar, Mayank;Tiwari, Ram;Veeranna, Thippeswamy
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.10
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    • pp.6141-6145
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    • 2013
  • Background: The objective of the study was to determine the knowledge, attitude and behaviors of the practicing dentists regarding tobacco cessation counseling (TCC) in Chhattisgarh state and also the barriers that prevent them from doing so. Materials and Methods: The study was conducted among dental practitioners of Raipur district, Chhattisgarh state (India). The sampling frame was registration with the State Dental Council and practicing in Raipur district. A questionnaire was personally administered and the practitioners were given explanations regarding how to complete it. Only descriptive statistics were calculated (SPSS version 16 for Windows). Results: Based on the responding dentists' self reports, 76% were not confident in TCC, 48% did not assume TCC to be their responsibility, 17% considered that it might have a negative impact on their clinical practice, whereas 24% considered it might take away precious time from their practice, 25% considered TCC by dentists to be effective to a considerable extent and 80% considered TCC activities are not effective due to lack of formal training, 69% considered dental clinics as an appropriate place for TCC but 82% thought there must be separate TCC centre and 100% of the dentists wanted TCC training to be a part of practice and that it should be included in dental curriculum. Some 95% of them were of the view that tobacco products should be banned in India and 86% responded that health professionals must refrain from tobacco habits so to act as role models for society. Conclusions: Dental professionals must expand their armamentarium to include TCC strategies in their clinical practice. The dental institutions should include TCC in the curriculum and the dental professionals at the primary and the community health care level should also be trained in TCC to treat tobacco dependence.

Molecular Characterization of a Chinese Cabbage cDNA Encoding Thioredoxin-h that is Predominantly Expressed in Flowers

  • Lee, Seung-Sik;Lee, Kyun-Oh;Jung, Bae-Gyo;Chi, Yong-Hun;Yoo, Ji-Young;Lee, Ji-Yeun;Lee, Jung-Ro;Park, Soo-Kwon;Kang, Soon-Suk;Jang, Ho-Hee;Lee, Sang-Yeol
    • BMB Reports
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    • v.34 no.4
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    • pp.334-341
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    • 2001
  • Even though three isotypes of thioredoxins (-f, -m and -h types) have been identified in a variety of plant cells, there are only a few reports on thioredoxin-h that were recently identified. In this study, a cDNA encoding a h-type of thioredoxin was isolated from a cDNA library of Chinese cabbage, and named here CTrx-h. An open reading frame of the gene contained a polypeptide of 133 amino acids with a conserved active center, WCGPC, which appeared in all of the thioredoxin proteins. A deduced amino acid sequence of the CTrx-h showed the highest sequence identity with those of Arabidopsis thioredoxin-h2 (75.2%) and thioredoxin-h5 (46.6%) proteins, but it shared a low sequence homology to other isotypes of plant thioredoxinm and thioredoxin-f. The CTrx-h protein that is expressed in E. coli represented not only an insulin reduction activity, but also electron transferring activity from NADPH to thioredoxin-dependent peroxidase. A genomic Southern blot analysis using the cDNA insert of CTrx-h revealed that the gene consisted of a small multigene family in Chinese cabbage genome. On the contrary to other thioredoxin-h proteins that were widely distributed in most tissues of the plant, the CTrx-h was predominantly expressed in flowers. The expression was very low in other tissues. The data of the Northern blot analysis suggests that the CTrx-h may have other functions in flower development or differentiation, in addition to its defensive role.

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