Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Gene Structure of Cotesia plutellae Bracovirus (CpBV)-$I_{k}B$ and Its Expression Pattern in the Parasitized Diamondback Moth, Plutella xylostella

프루텔고치벌 브라코바이러스(Cotesia plutellae Bracovirus) 유래 $I_{k}B$ 유전자 구조와 피기생 배추좀나방(Plutella xylostella) 체내 발현 패턴

  • Kim Yong-Gyun (School of Bioresource Sciences, Andong National University) ;
  • Basio Neil A. (Bulacan Agricultural State College, Pinaod, San Ildefonso) ;
  • Ibrahim Ahmed M.A. (School of Bioresource Sciences, Andong National University) ;
  • Bae Sung-Woo (School of Bioresource Sciences, Andong National University)
  • 김용균 (안동대학교 자연과학대학 생명자원과학부 곤충분자생리실험실) ;
  • ;
  • ;
  • 배성우 (안동대학교 자연과학대학 생명자원과학부 곤충분자생리실험실)
  • Published : 2006.04.01
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Abstract

Inhibitor kB (IkB)-like gene has been found in the genome of Cotesia plutellae bracovirus (CpBV), which is the obligatory symbiont of an endoparsitoid wasp, C. plutellae. The open reading frame of CpBV-IkB was 417 bp and encoded 138 amino acids. Four ankyrin repeat domains were found in CpBV-IkB, which shared high homology with other known polydnavirus IkBs. Considering a presumptive cellular IkB based on Drosophila Cactus, CpBV-IkB exhibited a truncated structure with deletion of signal-receiving domains, which suggested its irreversible inhibitory role in NFkB signal transduction pathway of the parasitized host in response to the wasp parasitization. CpBV-IkB was expressed only in the parasitized diamondback moth, Plutella flostella. Its expression was estimated by quantitative RT-PCR during parasitization period, showing a constitutive expression pattern from the first day of parasitization. An indirect functional analysis of CpBV-IkB was conducted and suggested a hypothesis of host antivirus inhibition.

프루텔고치벌(Cotesia plutellae)은 내부기생봉이고 kB억제자 (IkB)와 유사한 유전자가 이 기생봉의 절대 공생바이러스(C. plutellae bracovirus: CpBV) 게놈에서 발견되었다. 이 유전자의 발현 부위는 417 br의 크기이며 138개 아미노산 서열 정보를 포함하였다. 이 단백질은 4개의 ankyrin 반복영역을 지니고 있었으며, 알려진 다른 폴리드나바이러스 유래 IkB 유전자와 높은 상동성을 보였다. 초파리 Cactus 단백질을 통해 대상 기주 IkB와 비교하여 보면, IkB 신호수신영역이 부재하는 구조를 보여, CpBV-IkB는 NFkB 신호전달체계의 비가역적 억제 인자로 작용할 것으로 추정되었다. CpBV-IkB는 프루텔고치 벌에 기생된 배추좀나방에서만 발현되었다. 정량적 RT-PCR 방법으로 CpBV-IkB의 발현량을 조사하여 보면, 기생 첫날부터 발현을 보이기 시작하여 뚜렷한 발현량을 기생 전체 기간동안 유지하는 양상을 보였다. 이 CpBV-IkB의 기능 분석이 간접적으로 이뤄졌으며, 이 유전자 발현물이 대상 기주 항바이러스 억제 인자로 작용할 것이라는 가설을 제시하였다.

Keywords

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