• Journal of architectural history
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• v.15 no.4
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• pp.57-73
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• 2006

To begin with the purpose of protecting human life from the exterior in the prehistoric age, architecture was nothing but a shelter without any relation between the exterior and the intoner. But, today, with the community developed, architecture can not be resisted with her own function and have to change of the reciprocal one. Different with the oriental architecture, we can see, western architecture has developed in the stream of the form. The main idea of the space in terms of the relation, above all, is mostly concerned with relation between the exterior and the interior like between the architecture and the city. So the role of the facade in depth is the intermediation which consist to the relation in the exterior and interior of architecture. Considering a relation between two elements; the exterior and the interior, we can be inspired in the case of paintings, especially in the works of the Paul $C\acute{e}zanne$. $C\acute{e}zanne$ originally show the depth of space by different disposition of three parts : the background, the objects and intermediate space between the former. For instance, different with the other painters who put the background rear in the canvas and objects in the front of the background, $C\acute{e}zanne$ took the background and objects a same value and assure a depth in space by virtue of the intermediate space. Finally, by putting the background in forward of layer in the canvas and objects situated behind the background, the depth of the space can be occurred. Same as the idea of $C\acute{e}zanne$, Giuseppe Terragni and jean Nouvelle in architecture also intensify their effects through the activation of intermediate space between the interior facade and exterior frame. Not limited in the relation with the architecture and the city, space in relation gives us a higher quality of architectural promenade in depth.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2018.10a
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• pp.122-123
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• 2018

The purpose of this study is to investigate the problem of installing VR theme park in Korea and to find a solution for it. VR is expected to play a leading role in the fourth industrial revolution as a new industry, but it is a reality that it is hard to feel its value in actual field. This is a reality that can not move one step further because it is trapped in the existing legal network rather than the essence of VR because it sees VR from the existing law frame. Because VR is regarded as an amusement device in the amusement facility and is regarded as a game, the former is applied to the Tourism Promotion Law and the latter is applied to the game industry law. When one content and hardware are applied to both laws at the same time and other regulations are applied to it, it is practically impossible to operate VR properly. In order to solve this problem, it is necessary to revise each law little by little in each law and amend the law that can apply VR itself. This study is expected to contribute to the revitalization of VR industry by presenting the ultimate problems and solutions in creating VR theme parks in Korea.

• BMB Reports
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• v.35 no.2
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• pp.213-219
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• 2002

Malonyl-CoA decarboxylase (E.C.4.1.1.9) catalyzes the conversion of malonyl-CoA to acetyl-CoA. Although the metabolic role of this enzyme has not been fully defined, it has been reported that its deficiency is associated with mild mental retardation, seizures, hypotonia, cadiomyopathy, developmental delay, vomiting, hypoglycemia, metabolic acidosis, and malonic aciduria. Here, we isolated a cDNA clone for malonyl CoA decarboxylase from a rat brain cDNA library, expressed it in E. coli, and characterized its biochemical properties. The full-length cDNA contained a single open-reading frame that encoded 491 amino acid residues with a calculated molecular weight of 54, 762 Da. Its deduced amino acid sequence revealed a 65.6% identity to that from the goose uropigial gland. The sequence of the first 38 amino acids represents a putative mitochondrial targeting sequence, and the last 3 amino acid sequences (SKL) represent peroxisomal targeting ones. The expression of malonyl CoA decarboxylase was observed over a wide range of tissues as a single transcript of 2.0 kb in size. The recombinant protein that was expressed in E. coli was used to characterize the biochemical properties, which showed a typical Michaelis-Menten substrate saturation pattern. The $K_m$ and $V_{max}$ were calculated to be $68\;{\mu}M$ and $42.6\;{\mu}mol/min/mg$, respectively.

• BMB Reports
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• v.40 no.1
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• pp.15-21
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• 2007

Breast cancer is the most common malignancy among women, and mutations in the BRCA1 gene produce increased susceptibility to these malignancies in certain families. In this study, the forward 1-13 exons of breast cancer associated gene BRCA1 were cloned from breast cancer cell line ZR-75-30 by RT-PCR method. Sequence analysis showed that nine BRCA1 splice forms were isolated and characterized, compared with wild-type BRCA1 gene, five splice forms of which were novel. These splice isoforms were produced from the molecular mechanism of 5' and 3' alternative splicing. All these splice forms deleting exon 11b and the locations of alternative splicing were focused on two parts:one was exons 2 and 3, and the other was exons 9 and 10. These splice forms accorded with GT-AG rule. Most these BRCA1 splice variants still kept the original reading frame. Western blot analysis indicated that some BRCA1 splice variants were expressed in ZR-75-30 cell line at the protein level. In addition, we confirmed the presence of these new transcripts of BRCA1 gene in MDA-MB-435S, K562, Hela, HLA, HIC, H9, Jurkat and human fetus samples by RT-PCR analysis. These results suggested that breast cancer associated gene BRCA1 may have unexpectedly a large number of splice variants. We hypothesized that alternative splicing of BRCA1 possibly plays a major role in the tumorigenesis of breast and/or ovarian cancer. Thus, the identification of cancer-specific splice forms will provide a novel source for the discovery of diagnostic or prognostic biomarkers and tumor antigens suitable as targets for therapeutic intervention.

• BMB Reports
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• v.40 no.1
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• pp.22-28
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• 2007

MyoD, expressed in skeletal muscle lineages of vertebrate embryo, is one of muscle-specific basic helix-loop-helix (bHLH) transcription factors, which plays a key role in the determination and differentiation of all skeletal muscle lineages. In this study, a cDNA of grass carp MyoD was cloned and characterized from total RNA of grass carp embryos by RT-PCR. The full-length cDNA of grass carp MyoD is 1597 bp. The cDNA sequence analysis reveals an open reading frame of 825 bp coding for a protein of 275 amino acids, which includes a bHLH domain composed of basic domain (1-84th amino acids) and HLH domain (98-142th amino acids), without signal peptide. Then the MyoD cDNA of grass carp was cloned to yeast expression vector pPICZ$\alpha$A and transformed into P. pastoris GS115 strain, the recombinant MyoD protein with a molecular weight of about 31KD was obtained after inducing for 2d with 0.5% methanol in pH 8.0 BMGY medium, and the maximum yield was about 250 mg/L in shaking-flask fermentation. The results were expected to benefit for further studies on the crystal structure and physiological function of fish MyoD.

• BMB Reports
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• v.46 no.7
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• pp.358-363
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• 2013

In this paper, we firstly reported a C-type lectin cDNA clone of 1029 bps from the larvae of A. Pernyi (Ap-CTL) using PCR and RACE techniques. The full-length cDNA contains an open reading frame encoding 308 amino acid residues which has two different carbohydrate-recognition domains (CRDs) arranged in tandem. To investigate the biological activities in the innate immunity, recombinant Ap-CTL was expressed in E. coli with a 6-histidine at the amino-terminus (Ap-rCTL). Besides acted as a broad-spectrum recognition protein binding to a wide range of PAMPs and microorganisms, Ap-rCTL also had the ability to recognize and trigger the agglutination of bacteria and fungi. In the proPO activation assay, Ap-rCTL specifically restored the PO activity of hemolymph blocked by anti-Ap-rCTL antibody in the presence of different PAMPs or microorganisms. In summary, Ap-rCTL plays an important role in insect innate immunity as an pattern recognition protein.

• Computers and Concrete
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• v.7 no.3
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• pp.203-220
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• 2010

In the present study, exterior beam-column sub-assemblage from a regular reinforced concrete (RC) building has been considered. Two different types of beam-column sub-assemblages from existing RC building have been considered, i.e., gravity load designed ('GLD'), and seismically designed but without any ductile detailing ('NonDuctile'). Hence, both the cases represent the under-designed structure at different time frame span before the introduction of ductile detailing. For designing 'NonDuctile' structure, Eurocode and Indian Standard were considered. Non-linear finite element (FE) program has been employed for analysing the sub-assemblages under cyclic loading. FE models were developed using quadratic concrete brick elements with embedded truss elements to represent reinforcements. It has been found that the results obtained from the numerical analysis are well corroborated with that of experimental results. Using the validated numerical models, it was proposed to correlate the energy dissipation from numerical analysis to that from experimental analysis. Numerical models would be helpful in practice to evaluate the seismic performance of the critical sub-assemblages prior to design decisions. Further, using the numerical studies, performance of the sub-assemblages with variation of axial load ratios (ratio is defined by applied axial load divided by axial strength) has been studied since many researchers have brought out inconsistent observations on role of axial load in changing strength and energy dissipation under cyclic load.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.25 no.4
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• pp.363-372
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• 2012

In this study, a new information-based hybrid modeling framework is proposed. In the hybrid framework, a conventional mathematical model is complemented by the informational methods. The basic premise of the proposed hybrid methodology is that not all features of system response are amenable to mathematical modeling, hence considering informational alternatives. This may be because (i) the underlying theory is not available or not sufficiently developed, or (ii) the existing theory is too complex and therefore not suitable for modeling within building frame analysis. The role of informational methods is to model aspects that the mathematical model leaves out. Autoprogressive algorithm and self-learning simulation extract the missing aspects from a system response. In a hybrid framework, experimental data is an integral part of modeling, rather than being used strictly for validation processes. The potential of the hybrid methodology is illustrated through modeling complex hysteretic behavior of beam-to-column connections.

• Journal of Microbiology
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• v.38 no.2
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• pp.80-87
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• 2000

The nucleotide sequence of the luxC gene coding for lux-specific fatty acyl-CoA reductase and the upstream DNA (325bp)of the structural gene from bioluminescent bacterium, Photobacterium phosphoreum, has been deternubed. An open reading frame extending for more than 20 codons in 325 bp DNA upstream of luxC was not present in both directions. The lux gene can be translated into a polypeptide of 54 kDa and the amino acid sequences of lux specific reductases of P. phosphoreum shares 80, 65, 58, and 62% identity with those of the Photobacterium leiognathi, Vibrio fischeri, Vibrio harveyi, and Xehnorhabdus luminescenens reductases, respectively. Analyses of codon usage, showing that a high frequency (2.3%) of the isoleucine codon, AUA, in the luxC gene compared to that found in Escherichia coli genes (0.2%) and its absence in the luxA and B genes, suggested that the AUA codon may play a modulator role in the expression of lux gene in E. coli. The structural genes (luxC, D, A, B, E) of the P. phosphoreum coding for luciferase (${\alpha}$,${\beta}$) and fatty acid reductase (r, s, t) polypeptides can be expressed exclusively in E. coli under the T7 phage RNA polymerase/promoter system and identificationof the [35S]methionine labelled polypeptide products. The degree of expression of lux genes in analyses of codon usage. High expression of the luxC gene could only be accomplished in a mutant E. coli 43R. Even in crude extracts, the acylated acyl-CoA reductase intermediate as well as acyl-CoA reductrase activities could be readily detected.

• Archives of design research
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• v.12 no.4
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• pp.23-32
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• 1999

The 'Hard Matte' was the method being wide-screen to the directors wanted more vivid and made real films to the audience at the beginning of cinematography. The hard matte have changed as unique device in films as opening and closing credit titles making impressions which being captured strongly to the people at the time the wide-screen has developed. In HDTV has invented in NHK the hard matte is called 'letter box style and on music video channel we can see the style as easily. That kind of hard matte images on music video have effected to commercial films are seen recently. But some of commercial films have problems like only expending horizontal way. The hard matte is one of important ways that could regulate the art contents with art form. Korean film image makers as directors and production designers must know the role and function of hard matter has arranged reasonable in foreign films.