• Genomics & Informatics
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• 제2권2호
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• pp.67-74
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• 2004

Cells consistently face stressful conditions, which cause them to modulate a variety of intracellular processes and adapt to these environmental changes via regulation of gene expression. Hyperosmotic and oxidative stresses are significant stressors that induce cellular damage, and finally cell death. In this study, oligonucleotide microarrays were employed to investigate mRNA level changes in cells exposed to hyperosmotic or oxidative conditions. In addition, since heat shock protein 70 (HSP70) is one of the most inducible stress proteins and plays pivotal role to protect cells against stressful condition, we performed microarray analysis in HSP70-overexpressing cells to identify the genes expressed in a HSP70-dependent manner. Under hyperosmotic or oxidative stress conditions, a variety of genes showed altered expression. Down­regulation of protein phosphatase1 beta (PP1 beta) and sphingosine-1-phosphate phosphatase 1 (SPPase1) was detected in both stress conditions. Microarray analysis of HSP70-overexpressing cells demonstrated that diverse mRNA species depend on the level of cellular HSP70. Genes encoding Iysyl oxidase, thrombospondin 1, and procollagen displayed altered expression in all tested conditions. The results of this study will be useful to construct networks of stress response genes.

• 대한간호학회지
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• 제13권3호
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• pp.75-85
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• 1983

This study was done to research the relationship between general characteristics and stressor, stress situation, Nursing Intervention of pregnant woman. The subjects for this study were 70 pregnant woman from 3 clinics of Obstetrics and Gynecology located in J city. The data was collected through questionaires which was developed by Norman J. Chestnut. The content of the questionaire consist of following 3 categories; (1) stressor (2) stress situation (3) Mental and Physical support of husband. Analysis of data was done by use percentage, x²-test. (through EDPS) The findings of this study are as follows; 1, Stressors of pregnant woman are stresses associated with fetus, socioeconomic problem, herself, delivery, abortion and prematureity, husband, change of appearances, management of home, a woman's parents-in-law. Stress situations of pregnant woman are when she is doing on the heavy management of home, limit of life, socioeconomic discomfort, taking medicine, difficult relationship with a woman's parents-in-law, being sign of abortion and prematurity, her physical discomfort, family desire of delivery of son baby. 2. Husband's mental support that pregnant woman desired are careful husband's role, More love and interest about pregnant, cooperation of child reaing, maintenance of healthy life, understaning of diffical pregnancy, early return home, giving up drinking, positive feeling about changed appearance, unconcern about sex of fetus. And husband's mental supports that pregnant woman desired are cooperating of homework and childrearing, limit of intercouse, release behavior of pregnant woman's physical discern-fort, a walk, behavior with pregnancy together, socioeconomic stability. 3. Stressor of pregnant woman was significant with education, number of son, economic status, personality of husband, dowelling. (P<.05) And stress situation of pregnant woman was significant with personality of husband, dwelling, husband's desired sex .of baby. (P<.05) 4. Husband's mental and physical supports that pregnant woman desired was significant with number of daughter. (P<.05).

• 가정과삶의질연구
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• 제20권6호
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• pp.49-60
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• 2002

This study focused on family stresses and coping strategies among commuting couples. The participants of this study consisted of a national samples of 134 couples(268 individuals), who had been commuting for at least six months. The major findings obtained from the study are summarized in the following: First, the degree of family stresses among commuting couples was 2.98, when the maximal stress value was set to 5.0. No difference in the level of stress was detected from the roles of the wife and those of the husband. The most stressful aspect for the commuting couples came from the role of parents who care for their children. The second stressful aspect was their responsibility for their own parents, and finally the stress from the housework and maintenance of their marital lives followed next. There was no difference between the wives and husbands on the stressors. Secondly, the level of the coping strategies developed by the commuting couples themselves against their stresses was 3.37, when the maximal stress coping value was set to 5.0. Again, there was no difference in this level between wives and husbands. Among the strategies, they used ‘cognitive restructuring’the most frequently; sharing their responsibilities with other members in family life came next; and developing their own techniques to manage their stresses followed. Thirdly, the ages of the couple, the age of the first child, type of jobs, duration of the marriage and commute, and frequency of meeting affected the level of family stress among commuting couples. Finally, commuting couples developed quite different strategies to cope with their family stresses, and religion and family type affected significantly the type of coping strategies.

• Journal of Microbiology and Biotechnology
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• 제17권2호
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• pp.348-358
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• 2007

Metallothionein, a cysteine-rich stress response protein that is naturally induced by a variety of immunologic stressors, has been shown to suppress autoimmune disorders through mechanisms not yet fully defined. In the present study, we examined the underlying mechanisms by which metallothionein might mediate such regulation of autoimmunity. $Na\ddot{i}ve\;CD4^+$ T cells from metallothionein-deficient mice differentiated to produce significantly less IL-10, $TGF-{\gamma}$, and repressor of GATA, but more $IFN-{\gamma}$ and T-bet, when compared with those from wild-type mice. The levels of IL-4 and GATA-3 production were not different between the two groups of mice. Conversely, treatment with exogenous metallothionein during the priming phase drove $na\ddot{i}ve$ wild-type $CD4^+\;T$ cells to differentiate into cells producing more IL-10 and $TGF-{\beta}$, but less $IFN-{\gamma}$ than untreated cells. Metallothionein-primed cells were hyporesponsive to restimulation, and suppressive to T cell proliferation in an IL-10-dependent manner. Lymphocytes from metallothionein-deficient mice displayed significantly elevated levels of AP-1 and JNK activities in response to stimulation compared with those from wild-type controls. Importantly, transgenic mice overexpressing metallothionein exhibited significantly reduced susceptibility to collagen-induced arthritis and enhanced IL-10 level in the serum, relative to their nontransgenic littermates. Taken together, these data suggest that metallothionein is able to promote the generation of IL-10-and $TGF-{\beta}$-producing type 1 regulatory T-like cells by downregulating JNK-dependent AP-1 activity. Thus, metallothionein may play an important role in the regulation of Th1-dependent autoimmune arthritis, and may represent both a potential target for therapeutic manipulation and a critical element in the diagnostic assessment of disease potential.

• Journal of Preventive Medicine and Public Health
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• 제56권3호
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• pp.272-281
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• 2023

Objectives: Young mothers are vulnerable to postpartum depression due to role transition-related stress. Understanding the causes underlying these stressors is essential for developing effective interventions. Methods: This study analyzed the 2018 Indonesian Basic Health Research data. The Mini International Neuropsychiatric Interview was used to assess postpartum depression symptoms in mothers aged 15-24 years with infants aged 0-6 months. In 1285 subjects, the risk factors for postpartum depression were evaluated using multivariate logistic regression. Results: The overall prevalence of depression in the 6 months postpartum was 4.0%, with a higher prevalence in urban areas (5.7%) than in rural areas (2.9%). Urban and rural young mothers showed distinct postpartum depression risk factors. In urban areas, living without a husband (odds ratio [OR], 3.82; 95% confidence interval [CI], 1.24 to 11.76), experiencing preterm birth (OR, 4.67; 95% CI, 1.50 to 14.50), having pregnancy complications (OR, 3.03; 95% CI, 1.20 to 7.66), and having postpartum complications (OR, 5.23; 95% CI, 1.98 to 13.80) were associated with a higher risk of postpartum depression. In rural areas, postpartum depression was significantly associated with a smaller household size (OR, 3.22; 95% CI, 1.00 to 10.38), unwanted pregnancy (OR, 4.40; 95% CI, 1.15 to 16.86), and pregnancy complications (OR, 3.41; 95% CI, 1.31 to 8.88). Conclusions: In both urban and rural contexts, postpartum depression relates to the availability of others to accompany young mothers throughout the postpartum period and offer support with reproductive issues. Support from the family and the healthcare system is essential to young mothers' mental health. The healthcare system needs to involve families to support young mothers' mental health from pregnancy until the postpartum period.

• IMMUNE NETWORK
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• 제16권4호
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• pp.242-248
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• 2016

Thymic atrophy is a complication that results from exposure to many environmental stressors, disease treatments, and microbial challenges. Such acute stress-associated thymic loss can have a dramatic impact on the host's ability to replenish the necessary naïve T cell output to reconstitute the peripheral T cell numbers and repertoire to respond to new antigenic challenges. We have previously reported that treatment with the orexigenic hormone ghrelin results in an increase in the number and proliferation of thymocytes after dexamethasone challenge, suggesting a role for ghrelin in restraint stress-induced thymic involution and cell apoptosis and its potential use as a thymostimulatory agent. In an effort to understand how ghrelin suppresses thymic T cell apoptosis, we have examined the various signaling pathways induced by receptor-specific ghrelin stimulation using a restraint stress mouse model. In this model, stress-induced apoptosis in thymocytes was effectively blocked by ghrelin. Western blot analysis demonstrated that ghrelin prevents the cleavage of pro-apoptotic proteins such as Bim, Caspase-3, and PARP. In addition, ghrelin stimulation activates the Akt and Mitogen-activated protein kinases (MAPK) signaling pathways in a time/dose-dependent manner. Moreover, we also revealed the involvement of the FoxO3a pathway in the phosphorylation of Akt and ERK1/2. Together, these findings suggest that ghrelin inhibits apoptosis by modulating the stress-induced apoptotic signal pathway in the restraint-induced thymic apoptosis.

• 대한의생명과학회지
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• 제9권4호
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• pp.241-248
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• 2003

Sodium salicylate, a plant stress hormone that plays an important role(s) in defenses against pathogenic microbial and herbivore attack, has been shown to induce a variety of cell responses such as anti-inflammation, cell cycle arrest and apoptosis in animal cells. p38MAPK plays a critical role(s) in the cell regulation by sodium salicylate. However, the signal pathway for sodium salicylate-induced p38MAPK activation is yet unclear. In this study, we show that although sodium salicylate enhances reactive oxygen species (ROS) production, N-acetyl-L-cysteine, a general ROS scavenger, did not prevent sodium salicylate-induced p38MAPK, indicating ROS-independent activation of p38MAPK by sodium salicylate. Sodium salicylate-activated p38MAPK appeared to be very rapidly down-regulated 2 min after removal of sodium salicylate. Interestingly, sodium salicylate-pretreated cells remained fully responsive to re-induction of p38MAPK activity by a second sodium salicylate stimulation or by other stresses, $H_2O$$_2$ and methyl jasmonate (MeJA), thereby indicating that sodium salicylate does not exhibit both homologous and heterologous desensitization. In contrast, pre-exposure to MeJA, $H_2O$$_2$, heat shock, or hyperosmotic stress reduced the responsiveness to subsequent homologous stimulation. Sodium salicylate was able to activate p38MAPK in cells desensitized by other heterologous p38MAPK activators. These results indicate that there is a sensing mechanism highly specific to sodium salicylate for activation of p38MAPK, distinct trom pathways used by other stressors such as MeJA, $H_2O$$_2$ heat shock, and hyperosmotic stress.

• 한국사회복지학
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• 제36권
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• pp.199-228
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• 1998

맞벌이 부인의 가족지원 서비스 필요도를 결정짓는 요인을 조사하기 위해, 맞벌이 부인의 스트레스와 역할과중을 증대시키는 직장과 가정의 상황 구조적 요인들을 나타내는 여섯 가지 스트레스원, 역할과중, 스트레스, 네 종류의 맞벌이가족 지원서비스 (보육비지원 서비스, 직장내 보육서비스, 휴직제도, 유동근무제도)에 대한 필요도 간의 상호 관계를 설명할 수 있는 포괄적인 이론모델을 개발하였다. 맞벌이가족 지원서비스 필요도를 결정짓는 요인에 있어서의 계층간 공통점과 차이점을 비교하기 위해, 전문직 맞벌이 부인과 생산직 맞벌이 부인의 모델을 별도로 개발하여 비교하였다. 본 연구에서 개발된 모델은 진주.사천지역에 거주하는 234명의 전문직 맞벌이 부인과 208명의 생산직 맞벌이 부인으로부터 수집된 설문조사 자료를 통하여 검증되었다. 공변량 구조분석을 이용하여, 변인들 간의 구조관계를 설명할 수 있는 최적의 이론구조모델이 선정되었다 (전문직 모델: df=141, GFI=0.928, CFI=0.965; 생산직 모델: df=141, GFI=0.902, CFI=0.912). 자료분석 결과, 현재 이용 중인 보육서비스에 대한 불만족도가 높을 수록, 근무시간이 길 수록, 맞벌이 부인들의 역할과중은 증가하며, 역할과중은 스트레스를 증가시키는 것으로 나타났다. 스트레스가 증가할 수록, 맞벌이 부인들은 휴직제도를 필요로 하는 것으로 나타났다. 한편, 자녀가 어릴 수록 보육비 지원서비스를 필요로 하고 있으며, 자녀의 나이가 증가될 수록 유동 근무제도를 필요로 하는 것으로 나타났다. 보육비지원 서비스에 대한 필요도가 높을 수록 직장내 보육서비스에 대한 필요도가 높으며, 직장내 보육서비스의 필요도가 높을 수록 휴직제도에 대한 필요도가 높게 나타났다. 또한 휴직제도에 대한 필요도가 높을 수록 유동근무제도에 대한 필요도도 높게 나타났다. 이와 같은 전문직과 생산직 맞벌이 부인 모두에게 일반화될 수 있는 9가지 공통적 인과관계를 제외하고는 전문직과 생산직 부인의 서비스 필요도 결정요인에 있어 상당한 차이점이 발견되었다. 두 집단의 공통적 요인과 차별적 요인에 대한 논의를 바탕으로, 각 직장의 환경과 개인의 필요에 따라 맞벌이 부인이 가장 필요로 하는 서비스를 차별적으로 제공할 수 있는 방안이 제시되었다.

• 한국어류학회지
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• 제19권4호
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• pp.266-273
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• 2007

열충격 단백질(hsp)은 세포의 기능에 중요한 역할을 하는 보존성이 높은 단백질중의 하나이다. 이들 중 70 kDa 열충격 단백질은 외부의 자극과 관계없이 상시적으로 합성되는 HSC70 단백질과 외부의 자극에 반응하여 합성되는 HSP70 단백질이 있다. 본 연구에서는 넙치(Paralichthys olivaceus)의 70 kDa 열충격 단백질에 대한 cDNA를 아미노산 서열로 변환시켜 분석함으로써 이 유전자가 상시적으로 발현하는 열충격 단백질인 HSC70에 대한 유전자임을 밝혔다. Hsp70 유전자의 발현 기작을 조사하기 위하여 단백질 발현을 조절하는 5' 인접부위를 분리하고 이들의 염기서열을 분석함으로써 유전자 조절부위의 중요인자와 중심 부위를 동정하였다. 또한 Hsp70 유전자의 유전자 조절부위를 이용하여 형광단백질 발현벡터를 제작한 후 메다카 수정란에 미세 주입하여 배 발생 과정의 살아있는 메다카에서 발현하는 형광 단백질(GFP)의 발현을 조사하였다.

• 한국콘텐츠학회논문지
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• 제16권5호
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• pp.623-637
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• 2016

본 연구는 노인의 온라인 사회관계가 우울에 미치는 영향을 분석하는 데 목적이 있다. 서울 소재 복지관 2곳의 정보화교육프로그램(컴퓨터 기초반, 스마트폰반, 인터넷반)에 참여한 60세 이상 노인 총 144명을 대상으로 설문조사결과를 분석하였다. Williams(2006)의 사회자본척도(On and Off the 'Net' Scales for Social Capital in an Online Era)를 사용해 노인의 온라인 사회관계를 측정하였으며, 온라인 사회관계가 우울에 미치는 영향을 확인하기 위해 위계적 회귀분석을 실시하였다. 연구결과 인구사회학적 변수, 건강관련 변수, 스트레스 변수를 모두 통제한 상태에서도 온라인 결속형 사회관계는 노인의 우울에 영향을 미치는 것으로 나타났다. 이러한 연구결과는 지역 내 온라인 결속형 사회관계 강화 프로그램이 필요하며, IT교육 프로그램을 통해 온라인 결속형 사회관계를 강화시키는 것이 노인의 우울감소에 도움이 됨을 시사하고 있다.