• IMMUNE NETWORK
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• 제2권3호
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• pp.150-157
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• 2002

Background: Although Tat plays a role as a potent transactivator in the viral gene expression from the Human Immunodeficiency Virus type 1 long terminal repeat (HIV-1 LTR), it does not function efficiently in rodent cells implying the absence of a human specific factor essential for Tat-medicated transactivation in rodent cells. In previous experiments, we demonstrated that one of chimeric forms of TAR (transacting responsive element) of HIV-1 LTR compensated the restriction in rodent cells. Methods: To characterize the nature of the compensation, we tested the effects of several upstream binding factors of HIV-1 LTR by simple substitution, and also examined the role of the configuration of the upstream binding factor(s) indirectly by constructing spacing mutants that contained insertions between Sp1 and TATA box on Tat-mediated transactivation. Results: Human Sp1 had no effect whereas its associated factors displayed differential effects in human and rodent cells. In addition, none of the spacing mutants tested overcame the restriction in rodent cells. Rather, when the secondary structure of the chimeric HIV-1 TAR construct was destroyed, the compensation in rodent cells was disappeared. Interestingly, the proper interaction between Sp1 and TATA box binding proteins, which is essential for Tat-dependent transcription, was dispensable in rodent cells. Conclusion: This result suggests that the human-specific Tat cofactor acts to allow Tat to interact effectively in a ribonucleoprotein complex that includes Tat, cellular factors, and TAR RNA, rather than be associated with the HIV-1 LTR upstream DNA binding factors.

• BMB Reports
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• 제37권3호
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• pp.320-334
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• 2004

StMBF1 (Solanum tuberosum multiprotein bridging factor 1) is a plant member of the MBF1 family of transcriptional co-activators. In an attempt to understand the role of StMBF1, we analyzed its interaction with plant transcription factors of the homeodomain-leucine zipper (Hd-Zip) family, a group of proteins with a typical leucine zipper motif adjacent to a homeodomain. StMBF1 is able to interact in vitro with the Hd-Zip protein Hahb-4 both in the presence and absence of DNA. Upon binding, StMBF1 increases the DNA binding affinity of Hahb-4, and of another plant homeodomain containing protein from the GL2/Hd-Zip IV family, HAHR-1. The biological role of interactions is discussed in this paper.

• 한국환경과학회지
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• 제7권4호
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• pp.501-504
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• 1998

Vibrlo vulnificus가 병원성을 일으키는 한가지 요인으로서 iron의 역할을 본 연구에서 알아보고 V. vulnificus의 성장에 미치는 사람과 토끼 혈청에서의 iron과 $CCl_4$의 영향도 조사하였다. 쥐에 iron을 주사한 결과 50% 치사량이 감소하였고 감염 후 치사 시간도 감소하였으며 $CCl_4$,로 감염된 간에 의해서 혈청 중 iron준위는 증가하였다. 쥐를 치사시키는데 필요한 접종량은 혈청 중 iron준위와 직접적으로 상호 관련성이 있었다. Iron은 이 미생물이 포유류 혈청에서 살아남거나 성장하는 능력에 있어서 제한 요인으로 나타났다. 시험관 내에서와 생체 내에서 이들 결과는 iron이 V. vulnificus의 병원성에 있어서 중요한 역할을 한다는 강한 증거를 제공해주었다.

• Journal of Chest Surgery
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• 제33권1호
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• pp.1-6
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• 2000

Background: TNF-$\alpha$ plays a major role in producing left ventricular dysfunction cardio-myopathy pulmonary edema and inhibits the compensatory mechanism of congestive heart failure. IL-6 is an acute reactant of immune reaction and also known to control immune reaction but its function in the myocyte was not clearly investigated. Author's performed this experiment to investigate the contents of TNF-$\alpha$ and IL-6 on the assumption that TNF-$\alpha$ and IL-6 may reside in nonfailing heart that has gone cardiac surgery and play some role in cardiac function. Material and Method : Right auricular tissues were sampled from 12 patients who had undergone total corrective surgery for both congenital and acquired heart diseases from January 1998 to June 1998 in Kosin Universcfy Gospel hospital. The quantitive analysis of TNF-$\alpha$ and IL-6 were assessed by ELISA method in right auricular tissue. Hemodynamic values about the pressure of ventricle atrium aorta pulmonary artery and cardiac index pulmonary and systemic vascular resistance and cardiac output were measured by echocardiography and cardiac catheterization and biochemical analyses of LDH & AST were done before operation. statistical analysis was by Paired Student t-test. Patients were divided into children(under 15 years olds) and adults groups and the data was compared beween two groups. Conclusion: Mild pulmonary hypertension and increased pulmonary vascular resistance were existed in both group. The contents of tissue TNF-$\alpha$ IL-6 in each group were independent of each data.

• Molecules and Cells
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• 제21권3호
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• pp.356-359
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• 2006

The transcription factor E2F1 coordinates cell cycle progression and induces apoptosis in response to DNA damage stress. Aside from DNA damage, the role of E2F1 in the endoplasmic reticulum (ER) stress signaling pathways is unclear. We found that $E2F1^{-/-}$ murine embryonic fibroblasts (MEFs) are resistant to apoptosis triggered by the ER stress inducer thapsigargin. In addition, E2F1 deficiency results in enhanced phosphorylation of eukaryotic translation initiation factor $2{\alpha}$ ($elF2{\alpha}$). These results therefore indicate that E2F1 deficiency increases phosphorylation of $elF2{\alpha}$ in response to ER stress triggered by thapsigargin, and suggest that the reduction in ER stress-induced apoptosis in E2F1-deficient cells is related to the high level of $elF2{\alpha}$ phosphorylation.

• 한국가정과학회지
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• 제3권2호
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• pp.13-26
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• 2000

The purpose of present study were to find the general trends of mate selecting factors and. marital satisfaction. concentrated on married women, to examine the difference among mate selection factors and marital satisfaction according to socio-demographic variables and Psychological variables and to analyze the effects of these variables influencing marital satisfaction. The subjects were 276 wives, living in Kwangju that having passed under S years after marriage without divorce experience. The major findings were as follows . 1. In mate selection, factor of high-degree was personality. view of value. personal relations, achievement, emotional mature. self-differentiation. degree of affection's expression. sense of humor, charms and condition of health Respondents'marital satisfaction score showed 91.75 and this score was higher than median score(62.5) 2. The external factor of mate selection showed significant difference according to degree of education. career. order. and sex-role attitude. The internal factor of mate selection showed significant difference according to degree of education, career, order, self-differentiation, self-esteem, and sex-role attitude 3. As correlating mate selectional factors to marital satisfaction, the significance appears in the mate's personality. view of value, emotional mature. personal relations. self-differentiation, condition of health. achievement. charm, sense of humor and degree of affection's expression. 4. Married women's marital satisfaction was influenced by self-esteem, personality and child's number that were explained about 38% by these variables. In conclusion, to happy marital life must be loved her own self. and above all considered internal factors like personality than external factors in mate selection.

• 한국의류학회지
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• 제11권2호
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• pp.1-11
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• 1987

The purpose of this study was to develope a questionaire measuring clothing behavior of elementary school children. At first, after pretest, the clothing behavior questionaire consisted of 70 items which were devidad. into 7 subscales i.e. Clothing interest. Clothing satisfaction. Clothing management, Clothing sex-role. Clothing comfort. Clothing conformity. and Clothing independence. Each item was rated on a 3-point scale. Samples were 447 boys and girls (4 th, 5 th, 6 th grade) of three elementary schools in Seoul. Korea. The data were analyzed by item analysis and factor analysis. Factor analysis was useful in attempting to establish contruct validity. Item validity was examined based on the correlation coefficients and item discriminating power through the chi-square. Reliability was examined based on the Cronbach's Alpha Reliability Coefficient and test-retest method. With this analysis the subscales were reconstructed to following 6 factors. Clothing conformity items were not clustered by the factor analysis. 52 items of 6 factors selected by the analysis. The factors characteristics were as follows: 1. Clothing interest (10 items) 2. Clothing satisfaction (11 items) 3. Clothing management (8 items) 4. Clothing sex-role (12 items) 5. Clothing comfort (6 items) 6. Clothing independence (5 items)

• 한국조리학회지
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• 제23권4호
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• pp.144-162
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• 2017

This research utilized integrated package program of PASW Statistics 18.0 and AMOS 22.0 to analyze how the influence of service quality factor of coffee chains affects customer satisfaction and intention of customer behavior. The utilized statistics techniques were frequency analysis, exploratory, confirmatory factor analysis, covariance structure analysis, multiple group structure equation model analysis, etc. As a result of hypothesis verification, first of all, it was verified that the service quality factor of coffee chains affected positive (+) effect on service justice. Second, it was verified that the service justice affected positive (+) effect on customer satisfaction. To see it detailed, it is possible to see that all factors, perception of interactive justice, distributive justice, procedural justice, were adopted as the case of affecting service satisfaction (p<.001). Third, it was verified that among the circumstances that service quality affected to satisfaction, justice played a role as intermediary role. Fourth, it was verified that service satisfaction affected positive (+) effect on customer satisfaction, finally, the influence that customer satisfaction affecting behavioral intention has no effect on behavioral intention by brands of domestic or abroad.

• Animal cells and systems
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• 제7권3호
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• pp.173-186
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• 2003

The transition metal cadmium is a serious occupational and environmental toxin. To inhibit cadmium-induced damage, cells respond by increasing the expression of genes that encode stress-responsive proteins. The metal-regulatory transcription factor 1 (MTF-1) is a key regulator of heavy-metal induced transcription of metallothionein-I and II and other genes in mammals and other metazoans. Transcriptional activation of genes by MTF-1 is mediated through binding to metal-responsive elements in the target gene promoters. Phosphorylation of MTF-1 plays a critical role in the cadmium-inducible transcriptional activation of metallothionein and other responses. Studies using inhibitors indicate that multiple kinases and signal transduction cascades, including those mediated by protein kinase C, tyrosine kinase and casein kinase II, are essential for cadmium-mediated transcriptional activation. In addition, calcium signaling is also involved in regulating metal-activated transcription. In several species, cadmium induces heat shock genes. Recently much progress has been made in elucidating the cellular machinery that regulates this metal-inducible gene expression. This review summarizes these recent advances in understanding the role of some known cadmium-responsive genes and the molecular mechanisms that activate metal-responsive transcription factor, MTF-1.

• 한국항해항만학회지
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• 제31권2호
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• pp.133-139
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• 2007

Relative importance of maritime transport that takes charge of main current of freight in country' economy is very large. Especially, port and facility carry out important role which treats freight of import and export smoothly and improves international trade as turning point, to achieve key role on connection and association between sea and land. For such reason, enlargement of port facilities or development of port needs to grasp exactly the utilization of port, attributes and selective factors of shipper. On the other hand, the amounts of physical distribution on Mokpo port located in Korean west coast are increasing, with fast economic growth of East Asian including China. This study uses discrete choice model that is measuring to analyze attribute and characteristic of Mokpo port, and analyzes port selection by decision factors of shipper. This paper composed a questionnaire using the result of preceding research, to decide port selection factor among competitive ports. Through factor analysis on a basis of the questionnaire' result, five principal components were extracted. These are resorted out by Logit model, to grasp competitive elements of port. This research fin present direction which raises competitive power of ports in west coast of Korea, especially on alternative and concentration of middle-class port as Mokpo may be useful.