• 한국수산과학회지
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• 제42권4호
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• pp.322-328
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• 2009

Three-hundred and sixteen Escherichia coli strains from seawater, and a variety of farmed fishes, including oliver flounder (Paralichthys olivaceus), black rock fish (Sebastes schlegeli), red sea bream (Pagrus major) and sea bass (Lateolabrax japonicus) between May to October in 2004, were tested by agar dilution method to determine their susceptibility patterns to 17 antimicrobial agents. Overall, 92.1% of Escherichia coli isolates from samples showed antimicrobial resistance to at least one antimicrobial agent and the multiple resistance was seen in 173 isolates (54.7%). The resistance of E. coli isolates to tetracycline (74.1%) was highest, followed by cephalothin (69.9%), doxycycline (66.5%), streptomycin (47.2%), ampicillin (46.2%), cefazolin (31.6%), enrofloxacin (31.0%). norfloxacin (28.2%). The most frequent resistance pattern was TE-D-CF-CIP-ENO-NOR-AM-S-C-SXT-AmC-CZ (14.7%), followed by CF (6.2%), TE (5.1%), TE-CF (4.5%) in 177 isolates from fishes and TE-D-CF (7.2%) followed by TE-D-CF-S (5.8%), CF and TE-D-S (3.6%) in 139 isolates from seawater.

• 한국수산과학회지
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• 제31권5호
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• pp.707-712
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• 1998

한국 남해 연근해에서 어획된 12종의 주요 어류 (표$\cdot$중충회유어 5종: 멸치, 숭어, 전어, 방어, 고등어: 연안암초어 3종: 조피볼낙, 참돔, 감성돔: 저서어 4종: 도다리, 붕장어, 넙치, 말쥐치)를 대상으로 하여 그들의 일반성분조성과 CHOL 및 $\alpha$-Toc 함량의 계절변화를 검토하였다. 지질과 수분함량은 역의 상관관계(r=-0.86, p<0.001)로 계절에 따라 뚜렸한 변화를 나타내었으나, 단백질 및 회분함량은 거의 변화가 없었다. 멸치, 전어, 감성돔, 도다리, 붕장어, 넙치, 말쥐치는 각각 12월, 10월, 11월, 9월, 1월, 1월, 11월에 지질함량이 높았고, 산란기에 해당하는 계절에 지질함량이 낮아 산란의 영향이 큰 어종이었다. 그러나 숭어, 방어, 고등어, 조피볼낙, 참돔의 지질함량 변화는 산란의 영향보다는 수온과 섭이량의 영향이 큰 것으로 생각되었다. CHOL의 함량변화는 지질함량변화와 정의 상관관계 (r=0.89, p<0.001)를 나타냈으나, $\alpha$-Toc 함량은 계절에 따른 변화가 인정되지 않았다.

• 한국수산과학회지
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• 제19권2호
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• pp.147-154
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• 1986

양식방어(Seriola quinqueradiata)에 vibrio병이 발생하여 상당한 피해를 입고 있다. 본 연구는 양식방어에 발생한 vibrio병의 원인균에 대한 생화학적 성상, 생물학적 성상, 약제감수성및 병원성을 검토한 것으로 결과는 아래와 같다. 1. 병어의 신장에서 원인균을 분리하였으며 생화학적 성상, 생물학적 성상을 조사하여 Vibrio sp.로 동정하였다. 2. 분리균이 발육할 수 있는 NaCl농도는 $0.5{\sim}7\%$(최적농도; $3\%$), pH의 경우는 $6{\sim}10$(최적; 9)였다. 3. 분리균은 tetracycline, chloramphenicol, gentamycin에 감수성을 나타내었으나, sulfisomezole, sulfisoxazole이는 감수성이 없었다. 4. 수온에 따르는 병원성은 $25^{\circ}C$에서는 모든 접종어에 병원성을 나타냈으나, $15^{\circ}C$에서는 방어만 병원성을 나타냈다.

• 한국수산과학회지
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• 제41권6호
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• pp.435-439
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• 2008

The antimicrobial resistance of 160 strains of Enterococcus faecalis and 173 strains of E. faecium to 12 antimicrobial agents was investigated. The test strains were isolated from 126 wild seawater and farmed fish, including olive flounder (Paralichthys olivaceus), black rock fish (Sebastes schlegeli), red sea bream (Pagrus major), and sea bass (Lateolabrax japonicus), in 2005 and 2006. Overall, 91.9% of the E. faecalis isolates and 88.4% of the E. faecium isolates showed antimicrobial resistance to at least one antimicrobial agent. The pattern of antimicrobial resistance of the isolates differed little according to the species of fish. The percentage of E. faecalis and E. faecium with specific antimicrobial resistance differed according to the sample source. For the isolates from farmed fish samples, 66.7% of E. faecalis were tetracycline resistant and 54.5% of E. faecium were erythromycin resistant. By contrast, in the wild fish seawater samples, 92.0% of E. faecalis were rifampin resistant and 88.5% of E. faecium were tetracycline resistant.

• 한국수산과학회지
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• 제44권5호
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• pp.550-553
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• 2011

The proximate, fatty acid and total amino acid compositions of striped jewfish Stereolepis doederleini muscle were studied. The proximate composition was: 76.4% moisture, 19.4% protein, 2.92% lipid, and 1.19% ash. The predominant total lipid fatty acids were 16:0 (18.7%), 18:1n-9 (16.5%), 22:6n-3 (16.0%), 16:1n-7 (8.53%), 20:5n-3 (7.97%) and 18:0 (5.34%). Glutamic acid (17.4%), lysine (10.6%), aspartic acid (10.4%) and leucine (8.27%) were the predominant amino acids in striped jewfish muscle. These food components of striped jewfish were similar to those of costal and reef dwelling fishes such as black rockfish, black sea bream and rock trout, which are common sliced raw fish in Korea. Therefore, these results suggest that striped jewfish may represent a new aquaculture fish species.

• 한국수산과학회지
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• 제52권5호
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• pp.539-546
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• 2019

The major cultured marine fishes in sea off the coast Gyeongsangnam-do Province, South Korea, were assessed and included 9.3% rockfish Sebastes schlegelii, 7.8% red seabream Pagrus major, and 2.1% rock bream Oplegnathus fasciatus. The number of insurance payments related to disease mortality in cultured fish in 2017 was fourfold that in 2016. Economic loss in aquaculture due to disease in cultured fish is high and represents an important inhibitory factor affecting marine fishery productivity. In 2018, diseases led to severe production losses in several aquaculture species: 40.0% in rockfish, 11.4% in olive flounder Paralichthys olivaceus, 10.0% in filefish Thamnaconus modestus, and 9.3% in red seabream. Fish-parasitic pathogens such as Microcotyle sebastis, Alella spp., and Dactylogyrus spp. enter mainly via the gills and skin surface. Among bacterial pathogens, Vibrio species were most common, with Vibrio harveyi being the dominant species causing infections in these fishes. The bacterium Lactococcus garvieae is thought to exhibit host specificity in fish. The fish species in the present study exhibited a higher tendency for infection by heterologous pathogens than by a single pathogen; therefore, it is necessary to devise new strategies for treating diseases in cultured fish.

• 한국어병학회지
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• 제18권2호
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• pp.133-146
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• 2005

본 연구에서는 2002년에서 2004년간 우리 나라 해수 어류 양식장에서 분리되는 세균 중 비브리오 속에 속하는 세균의 종 조성을 조사하였다. 질병의 증상을 보이는 어류로부터 166개의 비브리오속 세균 균주를 수집하였으며, 이들 균주는 넙치 (133 균주), 조피볼락 (8 균주), 참돔 (6 균주), 대하 (5 균주), 감성돔 (4 균주), 전복 (3 균주) 및 기타 해수 어류 (7 균주) 등에서 분리한 균주를 대상으로 하였다. 모든 분리 균주에 대하여 각종 생화학적 성상을 조사하고 그 특성에 따라 분리 균주를 구분하였다. 각 생화화적 성상 group에서 대표 균주를 선정하여 16S rRNA 및 16S-23S rRNA 유전자 서열을 분석하고 이들 결과를 종합하여 분리 균주을 동정하였다. 그 결과 14종 이상의 비브리오 종으로 동정할 수 있었으며, 그 종 조성은 V. ichthyoenteri (45 strains), V. alginolyticus (34 strains), V. harveyi (32 strains), Ph. damselae subsp. damselae (Formerly V. damsela, 10 strains), V. campbellii (6 strains), V. costicola-like (6 strains), V. fisheri (5 stains), V. fluvialis (4 strains) 및 Vibrio spp. (24 strains) 등이었다.

• 한국수산과학회지
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• 제38권6호
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• pp.365-371
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• 2005

The antimicrobial resistance patterns to the 10 antimicrobial agents of potential pathogenic vibrios isolated from seawater and a variety of farmed fishes, including oliver flounder (Payalichthys olivaceus), black rock fish (Sebastes schlegeli), red sea bream (Pagyus major) and sea bass (Lateolabyax japonicus), were investigated from May to October, 2004. A total of 314 strains of the genus vibrios were isolated from 126 collected samples, and the number of isolated strains of Vibrio payahaemolyticus and Vibrio alginolyticus were 194 and 120, respectively. Apparently $98.5\%$ of V. parahaemolyticus and $100\%$ of V. alginolyticus isolates demonstrated antimicrobial resistance against at least one antimicrobial agent. The resistance of V. parahaernolyticus isolates to ampicillin ($97.9\%$) was highest, followed by oxolinic acid ($26.8\%$), amikacin ($19.1\%$) and tetracycline and amoxicillin/clavulanic acid ($6.7\%$). V. alginolyticus isolates were resistant to ampicillin ($100\%$), solfamethoxazoleit,imethopenem ($25\%$), amikacln ($21.7\%$), amoxicillin/clavulanic acid ($15.8\%$), ciprofloxacin ($13.3\%$), and tetracycline and doxycycline ($11.7\%$). The rate of multiple antimicrobial resistance to at least four antimicrobials was higher in the V. alginolyticus isolates ($20.8\%$) than in the V. parahaemolyticus ($6.7\%$).

• 수산해양교육연구
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• 제17권3호
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• pp.413-426
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• 2005

The objective of this study consists of facilitating seafood consumption by increasing its intake opportunities for consumer through analysis of sliced raw fish. A study on sliced raw fish consumption was conducted on 630 citizens of Busan(252 men and 378 women), attending high school and university, and employedas housekeepers and salaried employees. The goal of the study was to assess the attitude and degree of satisfaction obtained from eating sliced raw fish and ultimately to promote greater seafood consumption. A self-administered questionnaire was used to record the results. The results were as follows : About sixty percent of the respondents appeared to like or not to be reluctant to eating sliced raw fish. The main reasons for favoring sliced raw fish were its taste and nutritious effects. All ages liked sliced raw fish, but teenaged women, on average, tend to dislike sliced raw fish. The most preferred sliced raw fish was flounder. Most of the panelists preferred korean rock fish, and red sea bream due to their ordinary muscle. Sliced raw fish of the 2nd and 3rd variety were the most popular. For most of the sliced raw fish, the own-price elasticity came out to be relatively low. This can be explained by the fact that sliced raw fish is characteristically purchased based more on quality and freshness, than on price. SINGSINGWHE(SSW)-fresh sliced raw fish should be stored at 0$^{\circ}C$ for no longer than 10hrs. Taste, nutritional value and low price have the potential to promote the consumption of seafood. Factors which can reduce the consumption of sliced raw fish were safety due to Vibrio septicemia, rainy day, indistinct origin, and high rices. To promote sliced raw fish consumption, the subjects recommended the quality improvement and low price.

• 수산해양기술연구
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• 제42권2호
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• pp.69-77
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• 2006

The aim of this study was to develope a fishing technology for fisheries management for improving the survival rate of young fishes escaped from trawl net. Sea experiments were carried out to investigate the survival rate of the fishes in off Geomun island and Korean southern sea. Cover-net fish cage was designed and manufactured to collect fishes escaped from COD(codend) and TED(trawl escapement device). Fish cage was installed in a shallow site where scuba diver can observe the surviving fishes after separating the cage from trawl net. Most of fishes except horse mackerel(Trachurus japonicus) and mackerel(Scomber japonicus) could escaped through TED(bar space 35mm) easier than COD(mesh size 54mm). Especially, escapement rate of redlip croaker(Pseudosciaena polyactis) escaped from TED was the highest as 73.9%. Survival rate of horse mackerel(Trachurus japonicus) escaped from TED was 85.5% at the point of 96 hours over, while the survival rate for COD showed a rapid decrease according to the time elapse as 85.0% at the point of 24 hours, 76.4% for 48 hours, 62.7% for 72 hours and 56.1% for 96 hours. Over 90% of red seabream(Pagrus major) and rock bream(Oplegnathus fasciatus) escaped from TED were survived 96 hours over and the survival rate of those fishes were excellent compared with the other fish species. All korean pomfret(Pampus echinogaster) escaped from both COD and TED were died within 48 hours. Most of redlip croaker(Pseudosciaena polyactis) were also died within 12 hours because of the weak physiological characteristics by water pressure change. Survival rate of fishes like as horse mackerel(Trachurus japonicus) having hard skin was high while it was low for the fishes like as squid(Todarodes pacificus) and hair-tail(Trichiurus lepturus) having soft skin. We could know that there were big differences in the survival rate offish species escaped from trawl net.