• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2006년도 춘계 학술발표회 논문집
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• pp.738-743
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• 2006

Falling rocks are classed by the collapse to wide meaning. it is a kinds of earth disaster that has happen every year. Falling rocks are defined as blocks which had fallen from the cliff. It is caused by the relaxation of discontinuous structures like as a joint, a foliation, a bedding, and so on. Usually, the Relaxation of blocks from the rock face is happened by various kinds factors that are acted as a complex. Sometimes, the tree can be a factor of the weakness or relaxation of rock blocks and a cause of the falling rock.

• The Korean Journal of Physiology and Pharmacology
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• 제28권1호
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• pp.49-57
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• 2024

While arterial tone is generally determined by the phosphorylation of Ser¹⁹ in myosin light chain (p-MLC2), Thr¹⁸/Ser¹⁹ diphosphorylation of MLC2 (pp-MLC2) has been suggested to hinder the relaxation of smooth muscle. In a dual-wire myography of rodent pulmonary artery (PA) and mesenteric artery (MA), we noticed significantly slower relaxation in PA than in MA after 80 mM KCl-induced condition (80K-contraction). Thus, we investigated the MLC2 phosphorylation and the expression levels of its regulatory enzymes; soluble guanylate cyclase (sGC), Rho-A dependent kinase (ROCK) and myosin light chain phosphatase target regulatory subunit (MYPT1). Immunoblotting showed higher sGC-α and ROCK2 in PA than MA, while sGC-β and MYPT1 levels were higher in MA than in PA. Interestingly, the level of pp-MLC2 was higher in PA than in MA without stimulation. In the 80K-contraction state, the levels of p-MLC2 and pp-MLC2 were commonly increased. Treatment with the ROCK inhibitor (Y27632, 10 µM) reversed the higher pp-MLC2 in PA. In the myography study, pharmacological inhibition of sGC (ODQ, 10 µM) slowed relaxation during washout, which was more pronounced in PA than in MA. The simultaneous treatment of Y27632 and ODQ reversed the impaired relaxation in PA and MA. Although treatment of PA with Y27632 alone could increase the rate of relaxation, it was still slower than that of MA without Y27632 treatment. Taken together, we suggest that the higher ROCK and lower MYPT in PA would have induced the higher level of MLC2 phosphorylation, which is responsible for the characteristic slow relaxation in PA.

• 한국지반환경공학회 논문집
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• 제15권5호
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• pp.13-21
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• 2014

본 연구에서는 쉴드 TBM 공법의 다양한 세그먼트 모델링 기법에 따른 비교 해석을 통하여 해석기법에 따른 차이를 분석하고 이에 따른 합리적인 모델링 기법을 제안하였다. 또한 암반 이완하중 산정모델에 따른 하중 분석 및 3차원 유한요소해석을 통하여 실제 현장에서 적용 가능한 합리적인 하중산정 방법을 제시하였다. 암반분류에 의한 이완영역의 산정방법은 암반등급이 높은 지반에서는 결과의 차이가 크지 않으나 지반등급이 낮은 지반에서는 그 차이가 매우 크게 나타났으며, 3차원 해석에 의한 이완영역 산정결과 지반등급이 낮은 지반의 이완영역은 각 방법의 중간 정도 범위에 위치할 것으로 예측되었으며 지반등급이 낮은 지반의 세그먼트 계획 시에는 기계식 터널 굴착 특성을 반영한 3차원 해석을 통한 이완영역의 산정을 적극 검토할 필요가 있다.

• Geomechanics and Engineering
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• 제9권4호
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• pp.427-445
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• 2015

By means of finite element numerical simulation and pseudo-static method, the shallow-buried bilateral bias twin-tube tunnel subject to horizontal and vertical seismic forces are researched. The research includes rupture angles, the failure mode of the tunnel and the distribution of surrounding rock relaxation pressure. And the analytical solution for surrounding rock relaxation pressure is derived. For such tunnels, their surrounding rock has sliding rupture planes that generally follow a "W" shape. The failure area is determined by the rupture angles. Research shows that for shallow-buried bilateral bias twin-tube tunnel under the action of seismic force, the load effect on the tunnel structure shall be studied based on the relaxation pressure induced by surrounding rock failure. The rupture angles between the left tube and the right tube are independent of the surface slope. For tunnels with surrounding rock of Grade IV, V and VI, which is of poor quality, the recommended reinforcement range for the rupture angles is provided when the seismic fortification intensity is VI, VII, VIII and IX respectively. This study is expected to provide theoretical support regarding the ground reinforcement range for the shallow-buried bilateral bias twin-tube tunnel under seismic force.

• Geomechanics and Engineering
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• 제14권4호
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• pp.325-336
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• 2018

Deformation of rock masses is not only related to rock itself, but also related to discontinuities, the latter maybe greater. Study on crack propagation at discontinuities is important to reveal the damage law of rock masses. DDARF is a discontinuous deformation analysis method for rock failure and some modified algorithms are proposed in this study. Firstly, coupled modeling methods of AutoCAD-DDARF and ANSYS-DDARF are introduced, which could improve the modeling efficiency of DDARF compared to its original program. Secondly, a convergence criterion for automatically judging the computation equilibrium is established, it could overcome subjective drawbacks of ending one calculation by time steps. Lastly but not the least, relationship between the super relaxation factor and the calculation convergence is analyzed, and reasonable value range of the super relaxation factor is obtained. Based on these above modified programs, influences on crack propagation of joint angle, joint parameters and geo-stresses' side pressure are studied.

• 동의생리병리학회지
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• 제22권2호
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• pp.315-320
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• 2008

This study investigated the signaling mechanisms contributed to the vasodilatory effects of HMC05, a herbal prescription. HMC05 acted in an endothelium-independent manner. To elucidate the fundamental mechanisms of its vascular actions, we focused on the signaling molecules involved in actin-myosin filament regulation including 20 kDa myosin light chains (LC20), Rho-associated kinase (ROCK), PKC, JNK and extracellular signal-regulated protein kinase (ERK) in the endothelium-denuded thoracic aorta or isolated smooth muscle cells (SMCs). It lowered the phosphorylation level of LC20 and showed that ROCK, ERK, JNK and $PKC{\alpha}$ pathways played important roles in the effects, as confirmed by the observations with a specific inhibition or activation, and with the activity and the subcellular localization of these molecules. In particular, HMC05 dramatically inhibited the activity of ERK and the downstream signaling of ROCK. It also changed the subcellular localization of the phophorylated $PKC{\alpha}$ as well as the amount of phosphorylation. Taken together, these data indicate that the vascular relaxation effects of HMC05 are attributed to the regulation of these signaling mechanisms.

• Geomechanics and Engineering
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• 제33권1호
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• pp.53-63
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• 2023

Rock disturbance caused by blasting and stress relaxation is commonly observed during excavation. As the distance from the source of disturbance increases, the degree of disturbance decreases, and rock at a large depth does not experience disturbance. However, in stability analyses, a single value of disturbance is often applied to the entire rock mass, which leads to underestimated results. In this study, this modeling mistake is addressed by considering realistically varying rock disturbance. The safety of infinite slopes in a disturbed rock mass with a strength governed by the Hoek-Brown failure criterion is investigated based on the kinematic approach of limit analysis. The maximum disturbance is assigned to the outermost slope face because it is directly exposed to blasting damage and dilation, and the disturbance progressively decays with distance in the rock mass. The safety analysis results indicate that the assumption of uniform disturbance in the entire rock mass leads to underestimation of the rock strength and safety on infinite rock slopes. A critical slip surface appears to be within the disturbed rock layer as well as the interface between the disturbed upper rock and undisturbed lower rock.

• Biomolecules & Therapeutics
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• 제31권2호
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• pp.193-199
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• 2023

In this investigation, we made a study of the efficacy of luteolin (a flavonoid found in plants such as vegetables, herbs and fruits) on vascular contractibility and to elucidate the mechanism underlying the relaxation. Isometric contractions of denuded muscles were stored and combined with western blot analysis which was conducted to assess the phosphorylation of myosin phosphatase targeting subunit 1 (MYPT1) and phosphorylation-dependent inhibitory protein for myosin phosphatase (CPI-17) and to examine the effect of luteolin on the RhoA/ROCK/CPI-17 pathway. Luteolin significantly alleviated phorbol ester-, fluoride- and thromboxane mimetic-elicited contractions regardless of endothelial nitric oxide synthesis, implying its direct effect on smooth muscle. It also significantly alleviated the fluoride-elicited elevation in pCPI-17 and pMYPT1 levels and phorbol 12,13-dibutyrate-elicited increase in pERK1/2 level, suggesting depression of ROCK and PKC/MEK activity and ensuing phosphorylation of MYPT1, CPI-17 and ERK1/2. Taken together, these results suggest that luteolin-elicited relaxation includes myosin phosphatase reactivation and calcium desensitization, which seems to be arbitrated by CPI-17 dephosphorylation via ROCK/PKC inhibition.

• Biomolecules & Therapeutics
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• 제30권2호
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• pp.145-150
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• 2022

In this study, we investigated the influence of galangin on vascular contractibility and to determine the mechanism underlying the relaxation. Isometric contractions of denuded aortic muscles were recorded and combined with western blot analysis which was performed to measure the phosphorylation of phosphorylation-dependent inhibitory protein of myosin phosphatase (CPI-17) and myosin phosphatase targeting subunit 1 (MYPT1) and to evaluate the effect of galangin on the RhoA/ROCK/CPI-17 pathway. Galangin significantly inhibited phorbol ester-, fluoride- and thromboxane mimetic-induced vasoconstrictions regardless of endothelial nitric oxide synthesis, suggesting its direct effect on vascular smooth muscle. Galangin significantly inhibited the fluoride-dependent increase in pMYPT1 and pCPI-17 levels and phorbol 12,13-dibutyrate-dependent increase in pERK1/2 level, suggesting repression of ROCK and MEK activity and subsequent phosphorylation of MYPT1, CPI-17 and ERK1/2. Taken together, these results suggest that galangin-induced relaxation involves myosin phosphatase reactivation and calcium desensitization, which appears to be mediated by CPI-17 dephosphorylation via not PKC but ROCK inactivation.

• 터널과지하공간
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• 제17권4호
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• pp.255-265
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• 2007

폐광지역의 침하발생 메커니즘 및 영향범위는 현장의 지반조건, 지압분포, 채굴적의 기하학적인 조건, 상부 구조물의 하중 조건 등에 따라 달라지므로 이를 예측하기는 매우 어렵다. 또한 지질 및 지반 상태, 탄층의 발달 상태, 채굴방법 등이 채굴현장마다 다르므로 기존의 보강대책을 그대로 적용하는 데에는 한계가 있다. 본 연구에서는 국내 폐광지역에 대한 합리적인 보강대책 수립을 위해 광역 채굴현황조사를 바탕으로 채굴적의 분포를 파악하고, 대심도 시추조사, 탄성파 토모그래피, 시추공 영상촬영 등의 상세 지반조사를 수행하여 석탄층의 분포현황과 암반이완상태를 파악하였다. 이를 토대로 지반침하이론에 의해 침하발생 유형을 예측하고 지반침하량을 산정하였다. 또한, 연구대상 지역의 침하발생 메커니즘을 분석하였으며, 기존 폐광지역 보강사례를 분석하여 각 침하원인별로 대상지역의 지반특성에 부합되는 지반 및 구조물기초에 대한 보강대책을 수립하였다.