• Proceedings of the Korean Geotechical Society Conference
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• 2006.03a
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• pp.738-743
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• 2006

Falling rocks are classed by the collapse to wide meaning. it is a kinds of earth disaster that has happen every year. Falling rocks are defined as blocks which had fallen from the cliff. It is caused by the relaxation of discontinuous structures like as a joint, a foliation, a bedding, and so on. Usually, the Relaxation of blocks from the rock face is happened by various kinds factors that are acted as a complex. Sometimes, the tree can be a factor of the weakness or relaxation of rock blocks and a cause of the falling rock.

• The Korean Journal of Physiology and Pharmacology
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• v.28 no.1
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• pp.49-57
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• 2024

While arterial tone is generally determined by the phosphorylation of Ser¹⁹ in myosin light chain (p-MLC2), Thr¹⁸/Ser¹⁹ diphosphorylation of MLC2 (pp-MLC2) has been suggested to hinder the relaxation of smooth muscle. In a dual-wire myography of rodent pulmonary artery (PA) and mesenteric artery (MA), we noticed significantly slower relaxation in PA than in MA after 80 mM KCl-induced condition (80K-contraction). Thus, we investigated the MLC2 phosphorylation and the expression levels of its regulatory enzymes; soluble guanylate cyclase (sGC), Rho-A dependent kinase (ROCK) and myosin light chain phosphatase target regulatory subunit (MYPT1). Immunoblotting showed higher sGC-α and ROCK2 in PA than MA, while sGC-β and MYPT1 levels were higher in MA than in PA. Interestingly, the level of pp-MLC2 was higher in PA than in MA without stimulation. In the 80K-contraction state, the levels of p-MLC2 and pp-MLC2 were commonly increased. Treatment with the ROCK inhibitor (Y27632, 10 µM) reversed the higher pp-MLC2 in PA. In the myography study, pharmacological inhibition of sGC (ODQ, 10 µM) slowed relaxation during washout, which was more pronounced in PA than in MA. The simultaneous treatment of Y27632 and ODQ reversed the impaired relaxation in PA and MA. Although treatment of PA with Y27632 alone could increase the rate of relaxation, it was still slower than that of MA without Y27632 treatment. Taken together, we suggest that the higher ROCK and lower MYPT in PA would have induced the higher level of MLC2 phosphorylation, which is responsible for the characteristic slow relaxation in PA.

• Journal of the Korean GEO-environmental Society
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• v.15 no.5
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• pp.13-21
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• 2014

This study analyzed the differences in the analysis techniques through a comparative analysis of the various segment's modeling techniques of Shield TBM method and proposed reasonable modeling techniques. Also, this study suggested reasonable estimating methods of load to be applicable in the field through the load analysis and three-dimensional finite element analysis by estimating model of rock mass relaxation load. Estimating method of relaxation area by rock mass rating makes no odds of output in subgrade with high rock mass rating, but so the difference of output is large, that is judged to set conservative design off. In estimating result of rock mass relaxation area by three-dimensional analysis relaxation area of subgrade with low-grade soil was predicted to be positioned at medium-range of many methods, in case of designing segment in subgrade with low-grade soil it needs to actively review estimation of relaxation area through three-dimensional analysis reflecting mechanical tunnel excavation.

• Geomechanics and Engineering
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• v.9 no.4
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• pp.427-445
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• 2015

By means of finite element numerical simulation and pseudo-static method, the shallow-buried bilateral bias twin-tube tunnel subject to horizontal and vertical seismic forces are researched. The research includes rupture angles, the failure mode of the tunnel and the distribution of surrounding rock relaxation pressure. And the analytical solution for surrounding rock relaxation pressure is derived. For such tunnels, their surrounding rock has sliding rupture planes that generally follow a "W" shape. The failure area is determined by the rupture angles. Research shows that for shallow-buried bilateral bias twin-tube tunnel under the action of seismic force, the load effect on the tunnel structure shall be studied based on the relaxation pressure induced by surrounding rock failure. The rupture angles between the left tube and the right tube are independent of the surface slope. For tunnels with surrounding rock of Grade IV, V and VI, which is of poor quality, the recommended reinforcement range for the rupture angles is provided when the seismic fortification intensity is VI, VII, VIII and IX respectively. This study is expected to provide theoretical support regarding the ground reinforcement range for the shallow-buried bilateral bias twin-tube tunnel under seismic force.

• Geomechanics and Engineering
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• v.14 no.4
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• pp.325-336
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• 2018

Deformation of rock masses is not only related to rock itself, but also related to discontinuities, the latter maybe greater. Study on crack propagation at discontinuities is important to reveal the damage law of rock masses. DDARF is a discontinuous deformation analysis method for rock failure and some modified algorithms are proposed in this study. Firstly, coupled modeling methods of AutoCAD-DDARF and ANSYS-DDARF are introduced, which could improve the modeling efficiency of DDARF compared to its original program. Secondly, a convergence criterion for automatically judging the computation equilibrium is established, it could overcome subjective drawbacks of ending one calculation by time steps. Lastly but not the least, relationship between the super relaxation factor and the calculation convergence is analyzed, and reasonable value range of the super relaxation factor is obtained. Based on these above modified programs, influences on crack propagation of joint angle, joint parameters and geo-stresses' side pressure are studied.

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.2
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• pp.315-320
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• 2008

This study investigated the signaling mechanisms contributed to the vasodilatory effects of HMC05, a herbal prescription. HMC05 acted in an endothelium-independent manner. To elucidate the fundamental mechanisms of its vascular actions, we focused on the signaling molecules involved in actin-myosin filament regulation including 20 kDa myosin light chains (LC20), Rho-associated kinase (ROCK), PKC, JNK and extracellular signal-regulated protein kinase (ERK) in the endothelium-denuded thoracic aorta or isolated smooth muscle cells (SMCs). It lowered the phosphorylation level of LC20 and showed that ROCK, ERK, JNK and $PKC{\alpha}$ pathways played important roles in the effects, as confirmed by the observations with a specific inhibition or activation, and with the activity and the subcellular localization of these molecules. In particular, HMC05 dramatically inhibited the activity of ERK and the downstream signaling of ROCK. It also changed the subcellular localization of the phophorylated $PKC{\alpha}$ as well as the amount of phosphorylation. Taken together, these data indicate that the vascular relaxation effects of HMC05 are attributed to the regulation of these signaling mechanisms.

• Geomechanics and Engineering
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• v.33 no.1
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• pp.53-63
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• 2023

Rock disturbance caused by blasting and stress relaxation is commonly observed during excavation. As the distance from the source of disturbance increases, the degree of disturbance decreases, and rock at a large depth does not experience disturbance. However, in stability analyses, a single value of disturbance is often applied to the entire rock mass, which leads to underestimated results. In this study, this modeling mistake is addressed by considering realistically varying rock disturbance. The safety of infinite slopes in a disturbed rock mass with a strength governed by the Hoek-Brown failure criterion is investigated based on the kinematic approach of limit analysis. The maximum disturbance is assigned to the outermost slope face because it is directly exposed to blasting damage and dilation, and the disturbance progressively decays with distance in the rock mass. The safety analysis results indicate that the assumption of uniform disturbance in the entire rock mass leads to underestimation of the rock strength and safety on infinite rock slopes. A critical slip surface appears to be within the disturbed rock layer as well as the interface between the disturbed upper rock and undisturbed lower rock.

• Biomolecules & Therapeutics
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• v.31 no.2
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• pp.193-199
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• 2023

In this investigation, we made a study of the efficacy of luteolin (a flavonoid found in plants such as vegetables, herbs and fruits) on vascular contractibility and to elucidate the mechanism underlying the relaxation. Isometric contractions of denuded muscles were stored and combined with western blot analysis which was conducted to assess the phosphorylation of myosin phosphatase targeting subunit 1 (MYPT1) and phosphorylation-dependent inhibitory protein for myosin phosphatase (CPI-17) and to examine the effect of luteolin on the RhoA/ROCK/CPI-17 pathway. Luteolin significantly alleviated phorbol ester-, fluoride- and thromboxane mimetic-elicited contractions regardless of endothelial nitric oxide synthesis, implying its direct effect on smooth muscle. It also significantly alleviated the fluoride-elicited elevation in pCPI-17 and pMYPT1 levels and phorbol 12,13-dibutyrate-elicited increase in pERK1/2 level, suggesting depression of ROCK and PKC/MEK activity and ensuing phosphorylation of MYPT1, CPI-17 and ERK1/2. Taken together, these results suggest that luteolin-elicited relaxation includes myosin phosphatase reactivation and calcium desensitization, which seems to be arbitrated by CPI-17 dephosphorylation via ROCK/PKC inhibition.

• Biomolecules & Therapeutics
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• v.30 no.2
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• pp.145-150
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• 2022

In this study, we investigated the influence of galangin on vascular contractibility and to determine the mechanism underlying the relaxation. Isometric contractions of denuded aortic muscles were recorded and combined with western blot analysis which was performed to measure the phosphorylation of phosphorylation-dependent inhibitory protein of myosin phosphatase (CPI-17) and myosin phosphatase targeting subunit 1 (MYPT1) and to evaluate the effect of galangin on the RhoA/ROCK/CPI-17 pathway. Galangin significantly inhibited phorbol ester-, fluoride- and thromboxane mimetic-induced vasoconstrictions regardless of endothelial nitric oxide synthesis, suggesting its direct effect on vascular smooth muscle. Galangin significantly inhibited the fluoride-dependent increase in pMYPT1 and pCPI-17 levels and phorbol 12,13-dibutyrate-dependent increase in pERK1/2 level, suggesting repression of ROCK and MEK activity and subsequent phosphorylation of MYPT1, CPI-17 and ERK1/2. Taken together, these results suggest that galangin-induced relaxation involves myosin phosphatase reactivation and calcium desensitization, which appears to be mediated by CPI-17 dephosphorylation via not PKC but ROCK inactivation.

• Tunnel and Underground Space
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• v.17 no.4
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• pp.255-265
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• 2007

As the mechanism and effect range of subsidence are altered according to the various conditions (the ground condition, the earth pressure, the geometric condition of underground cavity and the structure load), the analysis and prediction of subsidence in abandoned mining area are very difficult. Also, as the geological characteristics and the mining methods are differed in each mines, the application of the pre-existing reinforcements without improvement has a lot of difficulties and limits. In this study, the various underground investigation such as long-depth core drilling, seismic tomography and BIPS (borehole image processing system) were performed, the distribution of underground cavity and coal seam and rock relaxation condition were analyzed. And we predicted the type of subsidence and estimated the subsidence by theories of mining subsidence. With these results, we analyzed the mechanism of subsidence occurrence in the research object area. Finally, we improved existing methods which were applied to the abandoned mining area and also we established the rational reinforcement for the ground and structure foundation against each subsidence cause.