• Mycobiology
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• v.36 no.4
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• pp.222-227
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• 2008

Upon studying the diversity of fungal endophytes associated with pine trees in Korea, many species of Penicillium were encountered. In this study, we report two species of Penicillium isolated from the needles of Pinus rigida. Based on ID region analysis, cultural and morphological characteristics, the two species were identified as Penicillium fellutanum and P. toxicarium, both of which are new to Korea.

• Journal of Korean Society of Forest Science
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• v.17 no.1
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• pp.23-28
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• 1973

In this survey, to estimate volume growth of pitch pine (Pinus rigida Mill) stand, diameter growth was estimated. Among 223 sample trees, the number of rejected trees was 12, about 5 percent of total sample trees. The stand showed uniform growth and rejected trees included insect-damaged trees. Compared with reports made on forest classification basis, pitch pine a single species showed faster growth. Minimum, average and maximum value of D.B.H and mean annual diameter growth, of 16 year old pitch pine stand, were as follow. The correlation of each factors estimated by 9590 of confidence interval is shown in the table of correlation as bellow.

• Journal of Korean Society of Forest Science
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• v.72 no.1
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• pp.16-26
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• 1986

Allozyme study on a small pitch pine stand originating and expanded rather rapidly from a few founder trees indicated that the colonization of the pitch pine population was made progressively from the place where the founder trees located to another by moving in cohorts of seeds from a limited number of family or genetically closely related family groups in line with the succeeding generations. This pattern of migration and colonization resulted marked differences in allelic and genotypic frequencies at many of the allozyme loci between the initially colonized subpopulation on the south-facing slope and the lately colonized subpopulation on north-facing slope of a hill. It appeared that gene fixation due to inbreeding and genetic drift occurred at some loci in the pitch pine population or subpopulations. However, even in t 1e inbreeding small pitch pine population or subpopulations, a comparatively large amount of genetic diversity or heterozygosity was maintained due to the high levels of gene recombination at many of the gene loci and natural selections favoring for heterozygotes.

• Proceedings of the Zoological Society Korea Conference
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• 1999.10b
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• pp.152.2-152
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• 1999

No Abstract, See Full Text

• Journal of Korean Society of Forest Science
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• v.55 no.1
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• pp.22-29
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• 1982

Two ectomycorrhizal fungi, Pisolithus tinctorius and Thelephora terrestris, were introduced form U.S.A. and inoculated to five pine species in Korea to evaluate the reported growth stimulation of host plants after inoculation. These fungi were grown as mycelial inoculum in large quantity and ioculated to the fumigated nursery soil just before seed sowing. At the end of the first growing season. Pisolithus stimulated the height growth of Pinus densiflora. P. thunbergii. P. rigida, and P. rigida x teada by55, 36, 69, and 37%, respectively, compared with control seedlings with no fumigation and no inoculation. When the growth stimulation was expressed with dry weight, Pisolithus increased dry weight of P. densiflora and P. rigida x taeda by 143% and 128%, respectively, over control seedling. Thelephora failed to stimulate growth of inoculated plants. Pinus koraiensis did not respond to the inoculation during the first growing season. It is concluded that artificial inoculation of nursery pine trees with selected mycorrhizal fungi should be seriously considered to improve the quality of planting stocks and to stimulate early plant growth. The potential for use of Pisolithus in reforestation on adverse sited is also discussed.

• The Plant Pathology Journal
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• v.24 no.2
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• pp.112-117
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• 2008

Pre-infection behavior of Fusarium circinatum on stems of pine species was investigated with scanning electron microscopy. Two-year-old stems of Pinus densiflora and p. rigida were inoculated with the fungal conidial suspension and subjected to $25^{\circ}C$ for up to 16 hr. Most microconidia germinated 12 hr after inoculation on pine stems. Conidia produced germ tubes from either one or both ends of microconidia. Germ tubes grew over the stem surface and appeared to enter host tissues through natural openings on pine stems. Surface cracks in the cork were entrance sites of germ tubes of F. circinatum. In addition, host cell wall cracks were often found at the tip of germ tubes. The cuticle appeared to be eroded either at the tip of germ tubes or around germlings. Germ tubes also produced appressoria-like structures, exhibiting swollen tips of germ tubes on the stem surface. There seems to be no significant differences in the pre-infection behavior of F. circinatum on stems between the two pine species.

• The Korean Journal of Ecology
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• v.26 no.6
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• pp.327-333
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• 2003

A mixed forest of pines (Pines densiflora and Pinus rigida) and an oak forest (Quercus mongolica) in Mt. Kwanak in Seoul were limed with 1.5 t/ha and 3 t/ha of dolomite in 1993 and investigated the effects of liming on the chemical changes in the 0-5 cm and 5∼10 cm layers of soil from 1994 to 1995. Soil pH values were greater in the limed plots than in the control plot by the unit of 0.1-0.6 in the pine forest and 0.1∼0.2 in the oak forest. Ca concentrations in the limed plots were greater than those in the control plots in both the pine and oak forests. A considerable increase in Mg concentrations in soil was observed in the limed plots. K and Na concentrations in the limed plots appeared to decrease in both the pine and oak forests two years after liming. Although Al concentrations were greater in the limed plots than in the control plot in the pine forest in 1994, there was a tendency of a decrease in the concentration of this metal in the limed plots in 1995. Mn concentrations also tended to decrease in the limed plots in both the pine and oak forests.

• Journal of Ecology and Environment
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• v.41 no.9
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• pp.265-270
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• 2017

Background: Over the years, pine pollens have been excluded as an allergen due to its relatively large size, low protein content, and waxy hydrophobic layer, despite their abundance. However, recent studies suggest the possibilities of pine pollens being allergens, and it has been reported that allergy symptoms were highly prevalent in areas with considerably large pine forests and high possibility of exposure to the pollen. Therefore, we conducted a comparative analysis of the allergenicities of the pollens from the dominant species of Korean pines, red pine (Pinus densiflora), black pine (Pinus thunbergii), and pitch pine (Pinus rigida), in mice. Methods: The protein composition of the pollens from the three pine species was compared via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The pine pollens and proteins extracted from the pollens were introduced to BALB/c mice by nasal inhalation and application to exposed skin and the IgE produced by the mice were extracted from blood and analyzed via ELISA. Results: SDS-PAGE showed differing protein compositions of the pollens of the three pine species. Analysis of blood IgE compositions showed a similar amount of IgE produced when pollens were applied to skin. In contrast, when mice inhaled the pollens, P. densiflora was shown to induce significantly more IgE production than those of the other two species. Conclusions: The experimental results demonstrate that the pollens of all three South Korean pine species induce IgE production, and this production was more pronounced when the pollens were inhaled than when they were applied to the skin. Of the three species, the pollen of P. densiflora was found to induce the highest level of IgE production.

• Journal of the Korean Wood Science and Technology
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• v.26 no.3
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• pp.33-40
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• 1998

The phenolic acids in needles of five pine species such as Pinus densiflora, P. thunbergii. P. virginiana. P. rigida. and P. koraiensis were analyzed seasionally (March, June, September and December) in order to investigate the resistant factors against pine gall-midge(Thecodiplosis japonensis Uchida et Inouye). The interrelation of resistance to pine gall-midge and the content of phenolic acids in pine needles was investigated in the artificial hybride pine species. The contents of salicylic acid in susceptible species (P. densiflora and P. thunbergii) to pine gall-midge which watered with salicylic acid solution in a pot was determined. The results can be concluded as follows, 1. There was a little change in total phenolic constituents of resistant and susceptible pines seasonally. The each content of benzoic acid, salicylic acid and p-hydroxybenzoic acid in June was the highest through four seasons. 2. In June, resistant species showed higher content of salicylic acid than susceptible species, while the content of gallic acid was the highest in December. 3. Among the 21 artificial hybrids(P. densiflora ${\times}$ P. virginiana. P. thunbergii ${\times}$ P. virginiana), the hybrides of the higher salicylic acid content showed the lower rate of pine gall formation. 4. Pine gall formation of the susceptible species which were watered with salicylic acid solution remarkably decreased.

• Journal of Ecology and Environment
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• v.33 no.2
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• pp.175-186
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• 2010

This study was designed to evaluate changes in the terpene composition of 3 types of pines (Pinus densiflora, Pinus thunbergii and Pinus rigida), while decomposing their leaf litter. Needle litters were placed at two different organic layer depths, one on the surface and the other beneath the litter layer. Changes in the terpene composition of this litter were detected using a gas chromatograph-mass spectrometer. Among the monoterpenes acquired from the fresh needles of P. densiflora and P. rigida, $\alpha$-pinene (12.05% and 19.87%, respectively) was the major one, followed by $\beta$-pinene (2.90% and 14.07%). However, from the needles of P. thunbergii, $\beta$-pinene (20.77%) was the major one, followed by $\alpha$-pinene (10.79%). Among the sesquiterpenes detected in P. densiflora, trans-caryophyllene (3.12%) was the highest composition compound, whereas germacrene-D (6.09%) for P. thunbergii and 1,6-cyclodecadiene (7.41%) and endo-1-bourbonanol (7.41%) for P. rigida were the highest content compounds. However, the total amounts of terpenes decreased sharply by 40-85.4% in all three types of pine needle after 90-120 days of the experiment. The concentration of each terpene differed during decomposition, and the majority of compounds disappeared from beneath the litter layer. It was determined that three types of reducing patterns of each compound appeared on the rate of loss of concentration during decomposition; one pattern decreasing sharply during the initial period, another pattern steadily or slowly decreasing, and a newly detected pattern at low concentration occurring during decomposition.