• 미생물학회지
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• 제30권2호
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• pp.124-128
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• 1992

한국 야산에서 포획한 275 수를 대상으로 형광항체법을 이용, Hantaan virus 에 대한 항체 보유 여부를 조사한 바 3.6% 가 양성으로 나타나 박쥐도 Hantaan virus 매개체란 사실이 세계최초로 확인되었고 박죄 종류별 항체 양성률을 보면 관박쥐(Rhinolophus ferrumeguinum) 가 4.3%, 안주애기박쥐 (Verperitilio superans) 가 10% 의 양성률을 모였으며 긴가락박쥐(Miniopterus schreibersii)와 큰수염박쥐 (Myotis mystatinus cilis)는 음성이었다. 또한 야생박쥐의 Rickettsia 종류별 향체보유율은 R. tsutsugamushi 가 3.3%, R. typhi 가 1.8%, R. sibirica 가 R. sibirica 가 3.3%였으며 Thai typhus-118 가 4.4% 로서 가장 높은 양성률을 보였다. 이상의 성적을 종합하여 보면 국내에 서식하는 박쥐는 신증후출혈열 및 리케치아의 보균 동물임이 최초로 밝혀져 보고한다.

• 대한바이러스학회지
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• 제28권4호
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• pp.377-382
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• 1998

Hemorrhagic fever with renal syndrome (HFRS), scrub typhus, murine typhus and leptospirosis have been the principal acute febrile diseases in Korea. To evaluate the seroepidemiologic patterns of acute febrile illness, sera collected from 2,423 patients in 1996 were examined for antibodies against Hantaan virus, Orientia tsutsugamushi, Rickettsia typhi, and Borrelia burgdorferi by indirect immunofluorescent antibody technique (IFA) and macroscopic agglutination test for Leptospira interogans. Seropositive cases against Otsutsugamushi, Rickettsia typhi, Leptospira interogans and Hantaan virus were 192 (7.9%), 193 (8.0%), 12 (0.5%) and 324 (13.4%), respectively. Male was more affected in HFRS and murine typhus contrasting to scrub typhus and leptospirosis in female. Most positive cases occurred during October and November for scrub typhus, and during November and December for HFRS. These results showed similar patterns with previous epidemical data for recent couple of years, and possibly implied no significant changes occurred in ecologic situations for acute febrile diseases in Korea.

• 대한바이러스학회지
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• 제28권1호
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• pp.1-9
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• 1998

To study the relation between ectoparasite, Rickettsia and hanntan virus in bats, four order of Athropoda were collected from Rhinolophus ferrumequinum captured in Cheju and Eptesicus serotinus captured in Chungnamin from July 1989 to March 1998. Also antibody of Rickettsia and hanntan virus were detected by immunofluoroscent antibody technique and RT-PCR. The results are as follows. 1. Five species of Acarina were identified from E. serotinus: Leptotrobidium subakamushi of Trombiculidae, Macronyssus coreanus, Steatonyssuss spinosuss and Steatonychus superans of Macronyssidae, Argas vespertilionis of Metastigmata. 2. Ischnopsyllus needhami of Siphonaptera and Cimex of Hemiptera were identified from E. serotinus. 3. Cycteribia uenoi and Brachytarsina kanoi of Diptera were identified from R. ferrumequinum. 4. The positive rate of rickettsial antibodies in E. serotinus were 17.58%, 15.15%, 22.22%, 52.73% against R. tsutsugamushi, R. typhi, R. sibirica and R. thai tick typhus, respectively. The high positive rate of antibody related to the high content of Arthropoda. 5. The Positive rate of hantaan virus IFA antibodies were 3.4% (27 of 802) and hanntan virus infection rate 36.7% (22 of 60) by RT-PCR in bats. According these result, we showed that certain species of Athropoda isolated playa role as vector of Rickettsia in E. serotinus. Also bats playa role as a reservoir of hantaan virus in nature.

• 한국군사과학기술학회지
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• 제22권4호
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• pp.575-580
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• 2019

In biological warfare, it is important to identify biological agents for proper treatment. We focused on developing a real-time RT-PCR kit that can detect multiple species of biological agents. AccuPower(R) Biothreat Real-Time RT-PCR Kit(v3.0) could detect Bacillus anthracis, Yersinia pestis, Vibrio cholerae, Francisella tularensis, Salmonella typhi, Rickettsia prowazekii, Variola virus, Hantaan virus, Yellow fever virus, Brucella spp., Shigella dysenteriae in a single reaction. The results showed that the kit was verified to be able to detect at least 0.005 ng of nucleotide and 10,000 CFU/ml of bacteria. Therefore, the kit is expected to be used as a rapid and sensitive detection kit for 11 species of biological agents within 2 hours.

• 한국동물위생학회지
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• 제26권3호
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• pp.203-214
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• 2003

Scrub typhus and murine typhus are common endemic febrile illness in the fall in Korea. Scrub typhus is caused by Orientia tsutsugomushi, murine typhus is caused by Rickettsia typhi. Trombiculid mites are known as both the vector and the reservoir host of O tsutsugamushi, the mites which transmit O tsutsugomushi have been reported to be Leptotrombidium pallidum and L scutellare. The author carried out an epidemiological study of scrub typhus and murine typhus in Kyodong-Myeon, Kanghwa-Gun, Incheon in relation to the residents and the host rodents, such as their distribution, seroepidemiology, and population density of chigger mites. 1. Out of 900 residents, 33(3.7%) showed positive reaction to O tsutsugamushi, 24(2.7%) to R typhi. 2. In the seropositives to O tsutsugamushi or R typhi, between the sixties and the seventies of the age were dominant. 3. In the seropositives to O tsutsugamushi serotypically Gilliam was dominant. 4. Among the total 42 field rodents trapped by the sherman traps, 18 rodents were Apodemus agrarius(42.9%), 13 rodents were Crocidura lasiura(31.0%), 5 rodents were Musmusculus(11.9%), 2 rodents(4.8%) were Crocidura suaveolens, Rattus norvegicus, Tscherskia triton, respectively. 5. Out of 42 field rodents, 25 were parasitized by 4,419 chigger mites, showing 59.5% of the infestation rate and 98.8 of the chigger index. L pallidum parasitized in A agrarius, C lasiura, M musculus, R norvegicus and T triton, and L scutellare parasitized only C lasiura. 6. Antibodies in the sera of field rodents against O tsutsugamushi and R typhi were investigated by indirect immunofluorescent antibody technique. Positive rate of antibody against O tsutsugamushi were 11.9(5 of 42) and all of the positive is A agrarius. Antibody against R typhi was not detected. These results might provide the basic information for the management of scrub typhus and murine typhus in Kyodong-myeon, where the epidemiological studies on scrub typhus and murine typhus was not carried out enough.

• Tuberculosis and Respiratory Diseases
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• 제52권4호
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• pp.367-375
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• 2002

연구배경: 발진열은 벼룩에 의해 매개되며 전세계적으로 발생되는 리케치아질환으로 대개 경한 임상경과를 보이나 간혹 중증으로 발현된다. 저자들은 발진열로 유발된 급성호흡곤란증후군 6예를 경험하게 되어 그 임상상을 분석하고자 하였다. 방 법: 1991년 이후 서울중앙병원에 발열을 주소로 내원하여 발진열로 진단된 환자들을 찾아 호흡곤란증후군을 동반한 군과 그렇지 않은 군으로 나누어 각군에 대한 연령, 성별, 혈액학적 검사 및 미생물학적 검사에 대해 조사하여 비교하였다. 발진열의 진단은 특이적 면역형 광항체검사법을 시행하여 초회 항체역가가 1:512 이상이거나 추적 항체가의 4배이상 증가가 있으면서 발진열에 합당한 임상양상이 있는 경우로 한정하였고 급성호흡곤란증후군은 American-European Consensus Conference에 제시된 기준에 따라 정의하였다. 결 과: 호흡곤란증후군이 동반된 6례를 포함 모두 15예가 있었다. 호흡곤란증후군이 동반된 6명의 환자 중 2명이 여자였고 3명이 도시에 거주하였다. 발진은 모두에서 관찰되지 않았다. 한명이 치료도중 사망하였다. 급성호흡곤란증후군과 연관된 인자로 진단 및 치료시까지의 지연시간과 저알부민혈증, 혈중 bilirubin의 증가, 높은 APACHE III 점수와 MOD 점수가 통계적 유의성을 보였다. 결 론: 발진열은 풍토병의 성격을 띤 지역에서 원인미상의 급성호흡곤란증후군의 한 원인으로 고려될 수 있고 최근 도시거주자에게도 발병되고 있다. 발진열에 의한 급성호흡곤란증후군의 예후는 다른 원인에 의한 경우보다 좋지만 조속한 진단 및 치료가 중요할 것으로 생각된다.