Anantasook, N.;Wanapat, M.;Cherdthong, A.;Gunun, P.
Asian-Australasian Journal of Animal Sciences
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v.26
no.6
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pp.820-826
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2013
The objective of this study was to determine the effect of rain tree pod meal with palm oil supplementation on feed intake, digestibility, microbial protein synthesis and microbial populations in dairy cows. Four, multiparous early-lactation Holstein-Friesian crossbred (75%) lactating dairy cows with an initial body weight (BW) of $405{\pm}40$ kg and $36{\pm}8$ DIM were randomly assigned to receive dietary treatments according to a $4{\times}4$ Latin square design. The four dietary treatments were un-supplementation (control), supplementation with rain tree pod meal (RPM) at 60 g/kg, supplementation with palm oil (PO) at 20 g/kg, and supplementation with RPM at 60 g/kg and PO at 20 g/kg (RPO), of total dry matter intake. The cows were offered concentrates, at a ratio of concentrate to milk production of 1:2, and chopped 30 g/kg of urea treated rice straw was fed ad libitum. The RPM contained condensed tannins and crude saponins at 88 and 141 g/kg of DM, respectively. It was found that supplementation with RPM and/or PO to dairy cows diets did not show negative effects on feed intake and ruminal pH and BUN at any times of sampling (p>0.05). However, RPM supplementation resulted in lower crude protein digestibility, $NH_3$-N concentration and number of proteolytic bacteria. It resulted in greater allantoin absorption and microbial crude protein (p<0.05). In addition, dairy cows showed a higher efficiency of microbial N supply (EMNS) in both RPM and RPO treatments. Moreover, NDF digestibility and cellulolytic bacteria numbers were highest in RPO supplementation (p<0.05) while, supplementation with RPM and/or PO decreased the protozoa population in dairy cows. Based on this study, supplementation with RPM and/or PO in diets could improve fiber digestibility, microbial protein synthesis in terms of quantity and efficiency and microbial populations in dairy cows.
Seo, Sung;Han, Dae-Duk;Jang, Sun-Sik;Kim, Won-Ho;Jung, Min-Woong;Choi, Jin-Hyuck;Kim, Jin-Sook;Kim, Ha-Young;Lee, Joung-Kyong
Journal of The Korean Society of Grassland and Forage Science
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v.32
no.1
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pp.1-8
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2012
This study was carried out in 2010 to investigate the utilization and forage quality of native grasses, such as $Phragmites$$communis$ which might reduce the cost of feeding domestic cattle. The regions surveyed were the Haenam ranch in Haenam, Pyeongchang, Wonju, and Yeonggwang. In Haenam, yearly silage production harvested from 300 ha was 2,000 MT (7 MT/ha). All of those round bale silages were self-consumed in that region, and marketing price was 50,000~55,000 won per roll (110 won/kg). $Phragmites$$communis$ of 150 cm in length contained 8.4% crude protein (CP) with relative feed value (RFV) 71.9 and 60.1% in vitro dry matter digestibility (IVDMD), which was somewhat more favorable than forage quality of rice straw. The silage quality of $Phragmites$$communis$ varied greatly by the time of harvest (CP 4.7~6.4%, RFV 62.2~78.9, and IVDMD 41.9~53.9%), even with the same district and of the same original forage materials. Generally, the quality of $Phragmites$$communis$ silage of was poorer than that of sorghum ${\times}$ sudangrass hybrid. In Pyeongchang, forage quality of $Phragmites$$communis$ was decreased from 13.9% to 7.6% in CP, 90.7 to 76.1 in RFV, and 72.9% to 54.7% in IVDMD, as plant was getting mature, from 79 cm to 117 cm, 121 cm or to 142 cm in length. In Wonju, the quality values of $Phragmites$$communis$ of 130 cm in length were 8.5% CP, 82.3 RFV and 70.2% IVDMD, while those of matured grasses of 220 cm in length were lower (10.2% CP, 65.1 RFV and 48.9% IVDMD), but this was a little more favorable than quality of rice straw. In Yeonggwang, feeding $Phragmites$$communis$ was tried in a Hanwoo feed, but stopped due to low profitability. In conclusion, the overall quality of most native grasses including $Phragmites$$communis$ in this survey was poor. Therefore, we recommend that $Phragmites$$communis$ and native grasses should be harvested on June or July to obtain richer forage quality in forage values than rice straw.
This study was conducted to investigate the effect of cattle manure application on productivity, feed value, and stock carrying capacity of forage crops in upland and paddy fields at Gang-Wondo area. In the result, dry matter yield of sorghum ${\times}$ sudangrass hybrids obtained was 15.12 ton/ha at the level of 150kg N/ha of composted cattle manure. Significantly highest values of crude protein and total digestible nutrients (TDN) yields obtained were 0.59 and 5.35 ton/ha at the level of 150kg N/ha of composted cattle manure in the first cutting, and 0.44 and 3.70 ton/ha at the level of 150kg N/ha of organic raw cattle manure in the second cutting, respectively. The values of $K_{CP}+K_{TDN}/2$ and $K_{ME}$ of sorghum ${\times}$ sudangrass hybrids obtained was 7.76 and 4.46 head/ha at the level of 150kg N/ha of composted cattle manure. The dry matter yield, crude protein and TDN yields of rice straw were 4.95, 0.16 and 2.75 ton/ha at the level of 100kg N/ha of organic raw cattle manure, and the values of $K_{CP}+K_{TDN}/2$ and $K_{ME}$ of rice straw were 1.89 and 3.43 head/ha. The dry matter yield of winter crops, rye+red clover was 4.36 ton/ha in upland field, and rye+hairy vetch was 4.19 ton/ha in paddy field at the level of 100kg N/ha of composted cattle manure. Crude protein and TDN yields of rye+red clover was 0.29 and 2.38 ton/ha at the level of 100kg N/ha of composted cattle manure in upland field, and rye+hairy vetch was 0.30 and 2.48 ton/ha at the level of 80kg N/ha of composted cattle manure in paddy field. The values of $K_{CP}+K_{TDN}/2$ and $K_{ME}$ of rye+red clover was 2.34 and 2.15 head/ha in upland field, and rye+hairy vetch were 2.27 and 2.11 head/ha in paddy field, respectively. As the result, the productivity, feed value, and stock carrying capacity of sorghum ${\times}$ sudangrass hybrids showed higher values with composted cattle manure than organic raw cattle manure. rye+red clover in upland field and rye+hairy vetch in paddy field were most adaptable mixed combinations for roughage production at Gang-wondo area, it may due to their highly productivity, feed value, and stock carrying capacity.
This study was conducted to investigate the optimal CP level in growing goat kids. Forty male goats were divided into four treatment groups fed diets containing CP 12, 14, 16, and 18% of concentrate feed, and rice straw, respectively. Results are summarized as follows. Average daily gain’s(ADG) of groups fed diets with CP 14% and CP 18% were 84.0 and 83.0g/d each, which were higher than group fed diets with CP 12% grown at 69.2g of ADG(P<0.05). Daily feed intakes of concentrate and rice straw were 590g and 45g each and there was no difference found between treatments. The amounts of feed required for body weight gain(g) were similar in the range of 7.0-7.3 for groups fed diets with CP 14%, 16% or 18%. But the group fed diets with CP 12% required somewhat more feed for gain at 8.8g. Dressing percentage of groups fed diet with CP 12% was 61.7%, which was higher than groups fed diets with CP 14% or CP 18%(P<0.05) but similar to that fed diet with CP 16%. Meat percentage from goats fed diet with CP 16% was 51.7%, which were higher than goats fed diets with CP 12% or CP 14%(P<0.05). Fat deposition of CP 12% group was higher than the other groups(P<0.05). Percentage of bone weight averaged 17.0% without any difference among treatment groups. Shear force and cooking loss, which are physical properties of goat meats, were lower in CP 16% and CP 14% groups than the other two groups. Water holding capacity of goats meats from CP 16% was higher than those from CP 18% group(P<0.05). Results from panel test showed that juiciness of meats from CP 16% group was higher than those from CP 12% group(P<0.05). Meats from CP 16% also was tested to be more tender than meats from the other groups. Results from this work suggest that the optimum crude protein level in growing goat's concentrate is 14-16% and that increase above this level seems not to improve meat production.
Wanapat, M.;Nontaso, N.;Yuangklang, C.;Wora-anu, S.;Ngarmsang, A.;Wachirapakorn, C.;Rowlinson, P.
Asian-Australasian Journal of Animal Sciences
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v.16
no.4
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pp.504-510
/
2003
Rumen ecology plays an important role in the fermentation process and in providing end-products for ruminants. These studies were carried out to investigate variations in rumen factors namely pH, $NH_3-N$ and microorganisms in cattle and swamp buffaloes. Furthermore, studies on diurnal patterns of rumen fermentation and the effect of rumen digesta transfer from buffalo to cattle was conducted. Based on these studies, diurnal fermentation patterns in both cattle and buffaloes were revealed. It was found that rumen NH3-N was a major limiting factor. Rumen digesta transfer from buffalo to cattle from buffalo to cattle was achievable. Monitoring rumen digesta for 14d after transfer showed an improved rumen ecology in cattle as compared to that of original cattle and buffalo. It is probable that buffalo rumen digesta could be transferred. However, further research should be undertaken in these regards in order to improve rumen ecology especially for buffalo-based rumen.
The objective of the study was to evaluate the effects of Lactobacillus, cellulase, and molasses on chemical composition, fermentation qualities, and microorganism count of sugarcane bagasse silage after 30-days fermentation. The treatments were arranged according to a factorial arrangement (2 × 2 × 2) + 1, in a complete randomized design. The first factor consisted of two levels of Lactobacillus casei TH14 (TH14, 0 and 0.05 g/kg fresh matter; the second factor consisted of two levels of cellulase enzyme (C, 0 and 104 U/kg fresh matter); and the third factor consisted of two levels of molasses (M, 0 and 5 g/ 100 mL distilled water). A treatment (+1) referred to the use of rice straw without any treatments. The result showed that dry matter increased by 4% and neutral detergent fiber decreased by 2% of sugarcane bagasse when ensiled as a combination of additives as compared to untreated sugarcane bagasse. The pH and ammonia nitrogen were significantly dropped to 3.5 and 2.3 g/kg dry matter. Furthermore, lactic acid was increased by 64% when compared to untreated sugarcane bagasse, respectively. Lactic acid bacteria count was increased by 28% as compared to untreated sugarcane bagasse. Based on this experiment, fermenting with L. casei TH14, cellulase, and molasses in combination resulted in the promotion of the best qualities of sugarcane bagasse silage.
The major limiting factor on the determination of combine capacity is the frequent occurence of clogging over the some parts of machine when the crop is wet in the case of Japanese self-feeding type combine. And in the case of American conventional combine having big separating parts, the great grain loss and damage occur when the machine is used for rice harvesting. This experiment was carried out to develop the new type threshing and separating equipment. Proto-type thresher which consist of a conical threshing drum and a conical separating sieve rotating around the threshing cone was constructed and tested. In the case of 800 rpm of threshing cone speed, average threshing loss was below 1 percent, separating loss was about 1 percent, grain damage was about 0.4 percent, and average total power required was about 2.6 PS. This design has some problems such as higher power required or wrapping problems under the conditions of feeding long damp straw. But, compared with the conventional combine or thresher, this machine certainly has some potentials for this approach to combine development. The crop feed rate must be increased through improvement of the feeding portion of the threshing cone. And it is required to investigate further about some parameters causing wrapping phenomena.
Information on the effects of different yeast species on ruminal fermentation is limited. This experiment was conducted in a $3{\times}4$ factorial arrangement to explore and compare the effects of addition of three different live yeast species (Candida utilis 1314, Saccharomyces cerevisiae 1355, and Candida tropicalis 1254) at four doses (0, $0.25{\times}10^7$, $0.50{\times}10^7$, and $0.75{\times}10^7$ colony-forming unit [cfu]) on in vitro gas production kinetics, fiber degradation, methane production and ruminal fermentation characteristics of maize stover, and rice straw by mixed rumen microorganisms in dairy cows. The maximum gas production (Vf), dry matter disappearance (IVDMD), neutral detergent fiber disappearance (IVNDFD), and methane production in C. utilis group were less (p<0.01) than other two live yeast supplemented groups. The inclusion of S. cerevisiae reduced (p<0.01) the concentrations of ammonia nitrogen ($NH_3$-N), isobutyrate, and isovalerate compared to the other two yeast groups. C. tropicalis addition generally enhanced (p<0.05) IVDMD and IVNDFD. The $NH_3$-N concentration and $CH_4$ production were increased (p<0.05) by the addition of S. cerevisiae and C. tropicalis compared with the control. Supplementation of three yeast species decreased (p<0.05) or numerically decreased the ratio of acetate to propionate. The current results indicate that C. tropicalis is more preferred as yeast culture supplements, and its optimal dose should be $0.25{\times}10^7$ cfu/500 mg substrates in vitro.
Chanjula, P.;Wanapat, M.;Wachirapakorn, C.;Rowlinson, P.
Asian-Australasian Journal of Animal Sciences
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v.17
no.10
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pp.1400-1410
/
2004
Eight crossbred (75% Holstein Friesian) cows in mid-lactation were randomly assigned to a switchback design with a 2x2 factorial arrangement to evaluate two nonstructural carbohydrate (NSC) sources (corn meal and cassava chips) with different rumen degradability and used at two levels of NSC (55 vs. 75%) with protein source (supplied by urea in the concentrate mix). The treatments were 1) Low degradable low level of corn (55%) 2) Low degradable high level of corn (75%) 3) High degradable low level of cassava (55%) and 4) High degradable high level of cassava (75%). The cows were offered the treatment concentrate at a ratio to milk yield at 1:2. Urea-treated rice straw was offered ad libitum as the roughage and supplement with 1 kg/hd/d cassava hay. The results revealed that total DM intake, BW and digestion coefficients of DM were not affected by either level or source of energy. Rumen fermentation parameters; NH3-N, blood urea nitrogen and milk urea nitrogen were unaffected by source of energy, but were dramatically increased by level of NSC. Rumen microorganism populations were not affected (p>0.05) by source of energy, but fungal zoospores were greater for cassava-based concentrate than corn-based concentrate. Milk production and milk composition were not affected significantly by diets containing either source or level of NSC, however concentrate than corn-based concentrate averaging (4.4 and 4.2, respectively). Likewise, income over feed, as estimated from 3.5% FCM, was higher on cassava-based concentrate than corn-based concentrate averaging (54.0 and 51.4 US$/mo, respectively). These results indicate that feeding diets containing either cassava-based diets and/or a higher of oncentrates up to 75% of DM with NPN (supplied by urea up to 4.5% of DM) can be used in dairy rations without altering rumen ecology or animal performance compared with corn-based concentrate.
This study was conducted to evaluate the effects of indigenous herbal supplements on growth, blood metabolites and carcass characteristics in the late fattening period of Hanwoo steers. In a 6 month feeding trial, thirty Hanwoo steers ($647{\pm}32$ kg) were allotted to one of 5 treatment groups, control (basal diet contained lasalocid), licorice, clove, turmeric and silymarin, with six steers per pen. All groups received ad libitum concentrate and 1 kg rice straw/animal/d throughout the feeding trial. Blood samples were collected at the beginning, middle, and the end of the experiment and the steers were slaughtered at the end. Blood glucose, triglyceride, total protein, and albumin concentrations were higher in the turmeric treatment compared with other treatments. Blood urea nitrogen and creatinine concentrations were highest (p<0.003 and p = 0.071, respectively) in steers treated with silymarin. Alanine aminotransferase activity was lower (p<0.06) for licorice and silymarin compared with the control group. There were no alterations in serum aspartate aminotransferase and gamma glutamyltransferase activities as a consequence of herb treatments (p = 0.203 and 0.135, respectively). Final body weight, body weight gain, average dairy gain and dry matter intake were not significantly different among treatments. Yield grade, marbling score and quality grade were higher for silymarin group than those of the control group (p<0.05). Therefore, the results suggest that silymarin can be used an effective dietary supplement as an alternative to antibiotic feed additive and a productivity enhancer, providing safe and more consumer acceptable alternative to synthetic compounds during the late fattening period of steers.
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