Proceedings of the Korean Society of Crop Science Conference
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2022.10a
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pp.78-78
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2022
Food productivity in North Korea is about 50% lower than in South Korea. In order to increase the productivity of major crops, it is necessary to develop early maturing, disease resistance, and high-yielding varieties and apply them early. Since the late 1990s, North Korea has been actively developing potatoes, rice and com as major food crops, and soybeans are considered important as a protein-supplying crop. Domestic cultivated varieties, which are expected to be most adaptable eco-climatologically, are mainly selected from soil with high nutrient soil. It is necessary to test separately for adaptability in low organic soil. So it is very necessary to apply technology to improve soil improvement through rotational crop selection in the middle and long-term. Therefore, this study was conducted to test the adaptability to low organic soils of domestic cultivars and to select varieties. In 2021 there are twenty two (22) varieties of soybeans were grown in low organic soil at the field of Chungbuk National University. This year twenty two (22) varieties of soybeans were also grown in low organic soil at the field of Chungbuk National University. Sowing was done on June 10, the planting distance was 70cm × 15cm, after opening the cotyledons fully, the soybeans were thinned and leaving two plants per hole. In addition, various types of growth characteristics and quantitative components were investigated to evaluate the adaptability to low organic soil of domestic varieties. This study was conducted to investigate the growth characteristics and quantitative components of soybean varieties grown in low organic soil. The flowering period of 22 varieties of soybeans was about 14 days from July 22 to August 4. The flowers of the beans were white, purple, light purple and the pubescence color was gray and brown where most of them were gray. The highest plant height was up to 130.4 cm and lowest was 20.3 cm, highest stem length was up to 119.5 cm and lowest was 15.3 cm. Highest first pod height (FPH) was up to 34.0 cm and lowest was 3.0 cm. Highest stem diameter was up to 15.76mm and lowest was 1.76 mm. Number of main stem nodes was up to 19 and at least 1. Number of branch was up to 10 and at least 0. The number of pod per plant was up to 121. Bacterial pustule has been spread in soybean field.
Salinity is one of the major abiotic stressors that inhibits the growth, yield, and productivity of crop plants. Therefore, it is necessary to develop crops with increased salt tolerance for cultivation in saline soils such as is found in reclaimed land. The objective of this study was to develop a salt-tolerant silage rice line that grows on reclaimed land. In order to develop this salt-tolerant silage rice, we transferred Saltol, a major QTL associated with salt tolerance, from IR64-Saltol, a salt-tolerant indica variety, into Mogyang, a susceptible elite japonica variety. To determine the effect of salt stress, Mogyang and IR64-Saltol cultivars were grown on a medium containing various concentrations of NaCl in in vitro conditions. Shoot length was found to decrease with increasing salt concentrations, and root growth was almost arrested at NaCl concentrations over 50 mM in the Mogyang cultivar. Based on these preliminary results, we screened five salt-tolerant lines showing superior growth under salt stress conditions. Polymerase chain reaction and sequencing results showed that the introgression types of Saltol QTL were derived from the IR64-Saltol cultivar in almost all selected lines. Based on the observed growth and physiological characteristics, the new Saltol introgression lines showed higher salt tolerance compared to the Mogyang parental cultivar. The salt-tolerant lines identified in this study could be used as a genetic resource to improve rice salt tolerance.
An experiment was conducted to obtain the basic information about competitive ability of rice against weeds as affected by eco-geographic race and plant spacing. Generally, more weeds were grown at the plot of Japonica-type cultivar, (Nakdongbyeo) compared to Tongiltype cultivar (Seogwangbyeo). The degree of weed suppression was highest at the 10 ${\times}$ 10cm plant spacing while no difference was observed between 30 ${\times}$ 15cm and 40 ${\times}$ (10 ${\times}$ 10 cm) plant spacings in both cultivar;. In all treatments except the plot of 10 ${\times}$ 10 cm plant spacing of Seogwangbyeo, singnificant amount of weeds was higher for no weeding plot compared to hand weeding plot. Grain yield loss due to weed competition was the highest at the 40 ${\times}$ (10 ${\times}$ 10cm) plant spacing and followed by 30 ${\times}$ 15cm and 10 ${\times}$ 10cm plant spacing, in order, for Seogwangbyeo. However, for Nakdongbyeo, the highest grain yield loss was obtained from the 30 ${\times}$ 15cm plant spacing and grain yield was not affected at the 10 ${\times}$ 10cm plant spacing. Rice grain yield was highly correlated with weed weight for both cultivars (Seogwangbyeo; Y = 5.44 - 0.0128 X, r = -0.929$^{**}$, Nakdongbyeo; Y = 4.96 - 0.0103 X, r = -0.934$^{**}$). To reduce grain yield by 50%, weed weight of 212.5g/sq.m for Seogwangbyeo and 241g/sq.m for Nakdongbyeo was needed, respectively. This result implied that Nakdongbyeo was more competitive against weeds compared to Seogwangbyeo.
The study was intended to know any relations between the rice tolerance to oxyfluorfen and varietal speciation in seed protein composition or any enzymatical allelies with or without chemical treatment. Rice varieties used were Chokoto, Aichiasahi, Agabyeo, IR 3941 and Tablei as the tolerant group, and Mushakdanti, Weld Pally, HP 1033, HP 857, and HP 907 as the susceptible, respectively. Electrophoretic methods used were SDS-PAGE for seed protein, 7% PAGE for isozymes (acid phosphatase and peroxidase from rice seedling) and changes in isoenzyme activity (malate dehydrogenase, peroxidase and esterase) as affected by oxyfluorfen treatment ($10^{-4}M$) was also studied. The results are summarized as follows. -Among 19 bands separated in seed proteins, two different rice groups selected in terms of tolerance were clustered in dissimilarity. This was based on 2 facts in that G band was not present in susceptible varieties and that less activity of H, N, O, P, Q, Rand S band was shown. -Among 4 bands separated in acid phosphatase, the presence of (band and lower activity of B band was specific for tolerant varieties. For 4 minor bands separated in peroxidase, the tolerant varieties had no activity in B band and higher activity in A, C, D bands. -Time-course study of isozymes as affected by $10^{-4}M$ oxyfluorfen showed that Chokoto, the tolerant varieties, had little activity in A band and consistently higher activities in Band C bands for malate dehydrogenase. For 5 bands separated in peroxidase, B band was not found in Chokoto while A, C, D, and E bands were consistently present. Esterase was separated into about 4 bands in which Chokoto had maintained higher activities in A, C and D bands.
Journal of the Korean Society of Food Science and Nutrition
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v.42
no.4
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pp.534-541
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2013
In the present study, the effects of heat treatments on the phenolic components and antioxidative activities of various rice cultivars (from Hwaseongbyeo, Keunnunbyeo, Hongjinjubyeo, and Heugkwangbyeo) were investigated. Each brown rice cultivar was heated at six temperatures (40, 60, 90, 120, 150, and $180^{\circ}C$) for 15 min. The total polyphenolic content (TPC) and total flavonoid content (TFC) of 70% ethanol extracts from heated brown rice were quantified using spectrophotometrical methods, and antioxidant activities determined using DPPH, ABTS radical scavenging activities and reducing power. Hongjinjubyeo had the highest TPC (6.50 mg GAE/g, DB) and ABTS radical scavenging activity (5.85 AAE/g, DB) at $60^{\circ}C$. Also, Heugkwangbyeo showed considerable values for TPC (6.57 and 6.89 mg GAE/g, DB) and ABTS radical scavenging activity (6.29 and 6.11 AAE/g, DB) at $40^{\circ}C$ and $180^{\circ}C$, respectively. Overall, the antioxidant activities of both Hongjinjubyeo and Heugkwangbyeo extracts had a strong positive correlation ($R^2{\geq}0.916$, ${\alpha}$=0.01) with TPC and TFC. These results indicate that heat treatment effectively enhances the antioxidant activity of Hongjinjubyeo and Heugkwangbyeo.
Journal of The Korean Society of Grassland and Forage Science
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v.33
no.2
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pp.87-93
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2013
'Okhan' (Avena sativa L.), an oat cultivar for winter use, was developed by the breeding team at the Department of Rice and Winter Cereal Crop, NICS, RDA in 2011. It was derived from an original cross between 'Early80', exhibiting early heading, and 'Maine PI-590' (CI 7518), exhibiting large-size grain, in 1995. Subsequent generations as well as cross-bred cultivars were handled in bulk, and pedigree selection programs took place at Suwon and Yeoncheon, respectively. A promising line, 'SO95027-B-45-16-10-6-2-Y7-10', was selected in 2004, and was designated 'Gwiri74' after being selected from a yield trial for three years from 2005 to 2008. The breeding line 'Gwiri74' was subsequently evaluated for earliness of heading and forage yield in four different locations, Yesan, Iksan, Kimjae, and Jeju, from 2009 to 2011, and was finally named as 'Okhan'. Over 3 years, the heading date of 'Okhan' was about 6 days earlier than that of the check cultivar 'Samhan', and their average forage dry matter yield harvested at the milk-ripe stage was 15.0 ton $ha^{-1}$, compared with 14.1 ton $ha^{-1}$ of check cultivar. Cultivar 'Okhan' was lower than the check cultivar 'Samhan' in terms of the protein content (9.2% and 9.9%, respectively) and total digestible nutrients (58.5%, and 59.3%, respectively), while the TDN yield per ha was more than the check (8.70 and 8.36 kg, respectively). Fall sowing cropping of 'Okhan' is recommended only in areas where average daily minimum mean temperatures in January are higher than $-7^{\circ}C$, and it should not be cultivated in mountain areas, where frost damage is likely to occur.
Cold plasma (CP) was applied to examine microbial safety and physicochemical properties of rough rice. CP was generated in a square-shaped plastic container (250 W, 15 kHz, ambient air) and dielectric barrier discharge plasma treatment was applied for periods of 0, 10, and 20 min during 2 weeks at 4 and $25^{\circ}C$. As a result of observing changes in growth of microorganisms, 3.46-3.86 log CFU/g of total aerobic bacteria and 2.27-2.86 log CFU/g of mold were detected in the early stage of storage. The growth of total aerobic bacteria and mold was increased depending on the storage temperature and period, but there was no big difference between cultivars. Microbial analysis after storage showed that microorganisms of plasma-treated group were less grown approximately 1.50 log CFU/g. Moisture content of rough rice was decreased by storage temperature and periods. As for the amylose content, changes in the content by plasma were not observed in Samkwang, Cheongpum and Misomi, whereas Palbangmi showed a tendency to increase. The results of this study indicated that CP treatment improved the microbial quality of rough rice, but further studies should be conducted to reduce the deterioration of sensory quality induced by CP.
Effect on blast lesion development was investigated in rice cultivar Milyang 23 and Jinheung using two races of Pyricularia oryzae compatible or incompatible to each of two rice cultivars. Discase lesions were measured in rice when inoculated with inoculum mixed with compatible and incompatible races, inoculated with a compatible race following with an incompatible race and vice versa. Lesions were smaller when rice was incoulated with an incompatible race and then inoculated with a compatible race than those inoculated in the reverse way both at the inoculated site and at 3cm-upper part from inoculated spot. The results suggested that induced resistance might play a role in suppression of lesion development although such an effect appeared to be influenced by heavy application of nitrogen fertilizer.
Choi, Myoung-Goo;Lee, Hyen-Seok;Hwang, Woon-Ha;Yang, Seo-Yeong;Lee, Yun-Ho;Lee, Chung-gun;Yun, Song Joong;Jeong, Jae-Hyeok
KOREAN JOURNAL OF CROP SCIENCE
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v.65
no.4
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pp.274-283
/
2020
Seed dormancy is an adaptive trait in which seeds do not germinate under unfavorable environmental conditions. Low dormancy seeds are easily germinated under optimal environmental conditions, and these characteristics greatly reduce the yield and quality of crops. In the present study, we compared the pre-harvest sprouting (PHS) rate of two cultivars, Joun and Jopyeong, using the Winkler scale after heading day and temperature of the test. The PHS rate increased as the Winkler scale after heading day increased from 700℃ to 1100℃ and the temperature of the test increased. In all conditions, the PHS rate of Jopyeong was higher than that of Joun. RNA-sequencing was used to analyze the cause of the high PHS rate. We analyzed the biological metabolic processes related to the abscisic acid (ABA) metabolite pathway using the KEGG mapper with selected differentially expressed genes in PHS seeds. We found that the expression of ABA biosynthesis genes (OsNCEDs) was down-regulated and that ABA catabolic genes (OsCYP707As) was up-regulated in PHS seeds. However, the quantitative real-time PCR results showed that Joun had a higher expression of OsNCEDs than that of Jopyeong, but OsCYP707As did not yield a significant result. Joun displayed higher ABA content than that of Jopyeong not only during ripeness time but also during PHS treatment. Taken together, we provided evidence that the ABA content remaining in the seed is important to the PHS rate, which is determined by the expression level of the ABA biosynthesis gene OsNCEDs.
In this study, we compared the levels of methylotrophic bacterial community diversity in the leaf, stem, grain, root and rhizosphere soil sainples of four rice cultivars collected from three regions of Korea. Thirty five pigmented and five non-pigmented isolates showing characteristic growth on methanul were obtained. When phylotypes were defined by performing numerical analysis of 42 characteristics, four distinct clusters were formed. While two clusters, I and IV diverged on the basis of nitrate and nitrite reduction, other two clusters, comprising only pink pigmented colonies, diverged on the basis of cellulase activity. Out of the two reference strains used in the analysis, Methyhbacterium extorquens AM1 diverged from all the clusters and M. fujisawaense KACC 10744 grouped under cluster III. All the isolates were positive for urease, oxidase, catalase and pectinase activity and negative for indole production, MR and VP test, $H_2S$ production, starch, and casein hydrolysis. No clusters were found to possess thermotolerant isolates, as no growth of the isolates was observed at $45^{\circ}C$. Two strains in cluster I were found to possess gelatin hydrolysis and methane utilizing properties respectively. Most of the isolates in all the four clusters utilized monosaccliarides, disaccharide and polyols as carbon source. Six isolates showed considerable nitrogenase activity ranging from 86.2 to $809.9nmol\;C_2H_4\;h^{-1}\;mg^{-1}$ protein.
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