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Changes in microbial and chemical properties of rough rice treated with cold plasma by storage temperatures and periods

저온 플라즈마 처리한 벼의 저장온도 및 기간에 따른 미생물학적 및 이화학적 특성 변화

  • Woo, Koan Sik (Crop Post-harvest Technology Division, Department of Central Area Crop Science, National Institute of Crop Science, RDA) ;
  • Yong, Hae In (Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute for Agriculture and Life Science, Seoul National University) ;
  • Jo, Cheorun (Department of Agricultural Biotechnology, Center for Food and Bioconvergence, and Research Institute for Agriculture and Life Science, Seoul National University) ;
  • Lee, Seuk Ki (Crop Post-harvest Technology Division, Department of Central Area Crop Science, National Institute of Crop Science, RDA) ;
  • Lee, Byong Won (Crop Post-harvest Technology Division, Department of Central Area Crop Science, National Institute of Crop Science, RDA) ;
  • Lee, Byoungkyu (Crop Post-harvest Technology Division, Department of Central Area Crop Science, National Institute of Crop Science, RDA) ;
  • Lee, Yu-Young (Crop Post-harvest Technology Division, Department of Central Area Crop Science, National Institute of Crop Science, RDA) ;
  • Oh, Sea-Kwan (Crop Post-harvest Technology Division, Department of Central Area Crop Science, National Institute of Crop Science, RDA) ;
  • Kim, Hyun-Joo (Crop Post-harvest Technology Division, Department of Central Area Crop Science, National Institute of Crop Science, RDA)
  • 우관식 (국립식량과학원 중부작물부 수확후이용과) ;
  • 용해인 (서울대학교 농생명공학부) ;
  • 조철훈 (서울대학교 농생명공학부) ;
  • 이석기 (국립식량과학원 중부작물부 수확후이용과) ;
  • 이병원 (국립식량과학원 중부작물부 수확후이용과) ;
  • 이병규 (국립식량과학원 중부작물부 수확후이용과) ;
  • 이유영 (국립식량과학원 중부작물부 수확후이용과) ;
  • 오세관 (국립식량과학원 중부작물부 수확후이용과) ;
  • 김현주 (국립식량과학원 중부작물부 수확후이용과)
  • Received : 2017.11.08
  • Accepted : 2017.11.23
  • Published : 2017.11.30

Abstract

Cold plasma (CP) was applied to examine microbial safety and physicochemical properties of rough rice. CP was generated in a square-shaped plastic container (250 W, 15 kHz, ambient air) and dielectric barrier discharge plasma treatment was applied for periods of 0, 10, and 20 min during 2 weeks at 4 and $25^{\circ}C$. As a result of observing changes in growth of microorganisms, 3.46-3.86 log CFU/g of total aerobic bacteria and 2.27-2.86 log CFU/g of mold were detected in the early stage of storage. The growth of total aerobic bacteria and mold was increased depending on the storage temperature and period, but there was no big difference between cultivars. Microbial analysis after storage showed that microorganisms of plasma-treated group were less grown approximately 1.50 log CFU/g. Moisture content of rough rice was decreased by storage temperature and periods. As for the amylose content, changes in the content by plasma were not observed in Samkwang, Cheongpum and Misomi, whereas Palbangmi showed a tendency to increase. The results of this study indicated that CP treatment improved the microbial quality of rough rice, but further studies should be conducted to reduce the deterioration of sensory quality induced by CP.

국내에서 생산되는 벼의 저장안전성 확보를 위한 기초기반연구로 플라즈마 기술을 이용하여 벼의 저장기간 및 온도에 따른 미생물 생육 및 성분 변화를 관찰하였다. 플라즈마 시스템은 컨테이너형 유전격벽 플라즈마로 공기방전방식을 이용하여 삼광, 청품, 미소미, 팔방미 품종을 0, 10 및 20분간 처리하여 $4^{\circ}C$, $25^{\circ}C$에서 2달간 저장하여 실험하였다. 미생물 생육 변화를 관찰한 결과 저장 초기에는 일반호 기성 미생물은 3.46-3.86 log CFU/g, 곰팡이는 2.27-2.86 log CFU/g이 검출되었다. 저장온도 및 기간에 따라 일반호기성 미생물 및 곰팡이의 생육은 증가하였으며, 품종간의 큰 차이는 없었다. 저장한 후의 미생물 분석 결과 플라즈마 처리군의 미생물이 약 1.50 log CFU/g 적게 생육되었다. 플라즈마 처리한 벼의 수분함량을 측정한 결과 플라즈마 처리에 의한 큰 차이는 관찰되지 않았으나, 저장온도가 올라가면 수분함량이 감소하는 것을 확인하였다. 지방은 플라즈마에 의해 감소하는 경향을 보였으나, 단백질 함량은 플라즈마 및 저장조건에 따른 일관적인 변화는 관찰되지 않았다. 아밀로스 함량의 경우 삼광, 청품, 미소미 품종은 플라즈마에 의한 함량 변화는 관찰되지 않았으나 팔방미는 증가하는 경향을 보였다. 이상의 결과를 종합하여 볼 때 플라즈마에 의해 벼의 저장안전성을 개선할 수 있으며 품질 변화의 최소화를 위하여 저온저장이 효과적이라고 판단된다.

Keywords

References

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