• 생약학회지
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• 제43권4호
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• pp.345-351
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• 2012

In this study, 49 Korean herbal medicines have been investigated with an in vitro evaluation system using glycation end products (AGEs) formation inhibitory activity. Of these, 18 herbal medicines ($IC_{50}$ < $50{\mu}g/ml$) were found to have significant AGEs formation inhibitory activity. Of these, five herbal medicines ($IC_{50}$ < $50{\mu}g/ml$) were found to have significant AGEs formation inhibitory activity. Particularly, Mallotus japonicus (twigs and leaves), Rhus javanica (twigs and leaves), Boehmeria nivea (whole plants), Quercus acuta (stems), and Eurya japonica (stems) showed more potent inhibitory activity (approximately 9-37 fold) than the positive control aminoguanidine ($IC_{50}=76.47{\mu}g/ml$).

• 한국염색가공학회지
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• 제24권4호
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• pp.260-269
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• 2012

The aim of this study was to develop higher-value added dyeing materials with the fermentation-dyeing -mordanting system using only the natural ingredients by integrating traditional fermentation techniques with traditional dyeing technique. Nuruk, which is used mainly to ferment traditional foods, was used as a fermenting agent to ferment 5 different types of materials. Acidic burnt alum and alkaline calcium hydroxide were used as natural mordants. The dyeability checked after fermentation showed that both cotton and silk mordanted with Gardenia jasminoides did not show notable changes, and 10 days of fermentation was found to be appropriate. Sophora japonica L. performed better on cotton materials mordanted with slaked lime, and alkaline mordants were found to be more effective than acidic ones. With Rheum coreanum, a fermentation period of more than 24 days ($5^{th}$ fermentation) worked best on cotton material, showing a 5 fold increase in the K/S value after the $5^{th}$ slaked lime fermentation than with no fermentation. Rhus javanica L. was found to increase the color fastness to gentle washing and the fastness to light was found to possess 4 means that natural dyed fabric have the highest level of durability, the grade 1 the lowest level of fastness.

• 생약학회지
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• 제44권2호
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• pp.193-199
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• 2013

We investigated the antioxidative and antimicrobial activities of the methanol extracts from Korean folk plants (MKs) in Chungcheong Province. Among 30 MKs, 16 plants at $100{\mu}g/ml$ showed over 90% scavenging activity of 1,1-diphenyl-2-picrylhydrazy (DPPH) and 30 plants exerted the hydroxyl radical scavenging effect over 55%. Fourteen plants at the concentration $50{\mu}g/30{\mu}l$ showed strong microbial inhibition activity against Escherichia coli and Staphylococcus aureus, with clear zone greater than 11 mm in disc assays. Furthermore, the protective effect against anti-inflammatory system using RAW 264.7 macrophage cell was also studied. The treatment of LPS & INF-${\gamma}$ to RAW 264.7 cell induced nitric oxide (NO), however inhibit the formation of NO less than 50% of 5 plants. The present result indicates that the 30 species of MKs exerts protective effect of oxidative stress, antimicrobial activities and anti-inflammatory. In particular, Rhus javanica and Cornus controversa showed stronger effect on not only radical scavenging activity and inhibits growth of S. aureus but also highest protective effects from inflammation.

• 한국식품저장유통학회지
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• 제12권3호
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• pp.267-274
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• 2005

천식과 관련하여 산화스트레스 (Oxidant stress)는 그 발병요인 중의 하나로 알려져 있다. 이에 본 연구는 농산물과 한약재를 이용하여 항산화 물질을 찾고자 하였으며, 시간과 비용의 단축을 위하여 HTS인 throughput screening)을 이용을 하였다. 항산화 실험과 관련하여서 DPPH(1,1-diphenyl-2-picrylhydrazyl), FRAP(ferric ion reducing antioxidant power), HO(hydroxyl radical) 소거, linoleic acid에 대한 항산화 활성 등을 시행하였다. 이후 $H_{2}O_2$에 의한 산화스트레스를 이용한 세포사멸을 유도하여 세포생존을 확인해 보았다. 실험결과 해바라기씨(Helianthus annuus), 신선초(Angelica utilis Makino), 시금치(Rehmannia glutinosa Libo) 등이 활성이 높게 나타났다. 본 연구는 여기에서 나온 hit를 이용하여 동물모델 실험을 진행하고자 한다.

• 대한수의학회지
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• 제43권4호
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• pp.625-631
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• 2003

The extracts from 40 different traditional medicinal herbs were used to investigate the antimicrobial activities against Escherichia coli. Among them, the extracts from Paeonia suffruticosa (PS), Siegesbeckia orientalis (SO), Schizandra chinensis (SC), Caesalpinia sappan (CS) and Rhus javanica (RJ) exhibited high antimicrobial activities against E. coli, Minimum inhibitory concentrations (MIC) of the RJ extract against E. coli were 0.8 mg/ml. After heating treatment of these extracts, the antimicrobial activities against E. coli were significantly reduced in case of the CS extract. After alkaline or acid treatment of these extracts, the antimicrobial activities against E. coli were significantly increased in the PS extract but reduced in both SO and RJ extract. Since extracts from RJ and CS exhibited the highest antimicrobial activities, bacterial growth-inhibiting activities against E. coli by these two extracts were further examined. Optical density at 620 nm after 24 hours incubation of E. coli in the presence of 100, 300 or 500 ppm of RJ extract ranged from 0.1 to 0.2 compared to 0.35~0.65 in the absence of RJ extract, indicating that growth of E. coli was significantly inhibited within 24 hours by the addition of at least 100 ppm of RJ extract. Optical density at 620 nm after 24 hours incubation of E. coli in the presence of 300 or 500 ppm of CS extract ranged from 0.01 to 0.25 compared to 0.5~0.55 in the absence of CS extract, indicating that growth of E. coli was also significantly inhibited within 24 hours by the addition of at least 300 ppm of CS extract. In conclusion, these findings suggest that extracts from RJ and CS may play important roles for antimicrobial activities against E. coli causing various animal diseases.

• Journal of Forest and Environmental Science
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• 제30권1호
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• pp.167-178
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• 2014

The populations of Pinus densiflora in South Korea were grouped into 4 types; Community of Pinus densiflora-Quercus serrata, Community of Pinus densiflora-Quercus mongolica, Community of Pinus densiflora-Fraxinus sieboldiana & Rhus javanica & Quercus mongolica and Community of Pinus densiflora-Prunus sargentii & Rhododendron mucronulatum. The Shannon diversity index was varied between 0.9171 and 1.5016, while the Similarity index was varied between 43.18 and 72.16. The species of Pinus densiflora are correlated positively significant with Carex humilis var. nana, Quercus serrata and Atractylodes ovata and negatively significant with Aster scaber and Sorbus alnifolia in order. According to the Correlation analysis, the relationship between the distribution of Pinus densiflora populations and the environment factors are closely related with the character of forest soil. Thus types for Pinus densiflora are differed. pH, Ex. K and Ex. Ca of Pinus densiflora populations effect No. of species and H'(shannon index). Canopy open effects No. of Individuals of Pinus densiflora community.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2012년도 정기총회 및 춘계학술발표회
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• pp.15-15
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• 2012

Obesity is a physical condition that results from excessive storage of fat in the body. The present study examined the anti-obesity effects of the selected natural medicine, Galla rhois extract (GRE) and solvent fractions on 3T3-L1 preadipocytes and in vivo studies. Here, we show that EtOAc fraction of Galla rhois inhibits the differentiation of the 3T3-L1 preadipocytes induced by differentiated medium in a dose-dependent manner. To investigate the effect of the GRE-EtOAc fraction on obesity in high fat diet-fed C57BL/6 mice, which included a normal diet (ND), high-fat diet (HFD) and HFD+GRE concentration-dependent, were fed to the mice for 6 weeks. The GRE-EtOAc fraction was inhibited the highest adipocyte differentiation in vitro, the GRE supplement significantly decreased body weight and visceral fat mass compared to the HFD group. The total cholesterol and triglyceride levels in the plasma were significantly decreased by GRE supplementation compared with those of the HFD group. Also, we aimed to determine the differentiation inhibition and the modulation of differentiation genes brought about by the Galla rhois in adipocyte. A cDNA microarray-based method was introduced for the high contents screening (HCS) of gene expressions. This technology has revolutionized gene expression studies by providing the means to measure mRNA levels in thousands of genes simultaneously in simple and complex biological samples. 13 genes were founded to be affected in their expression levels by more than 5-fold up-regulation after 4 days treatment with the EtOAc fraction from Galla rhois. Otherwise, 21 genes were founded to be affected in their expression levels by more than 5-fold down-regulation treated with the EtOAc fraction. Therefore, Galla rhois extract may be considered for use in a therapeutic agent to control obesity.

• 한국환경복원기술학회지
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• 제21권5호
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• pp.81-96
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• 2018

The purpose of this study is to analyze the characteristics of vegetation changes and structures in the buffer green zone of Sihwa Industrial Complex and propose environmental, ecological and multi-functional vegetation management directions. The density of the Pinus thunbergii decreased from $23.1trees/100m^2$ in 2006 to $9.6trees/100m^2$ in 2017 due to the influence of thinning works. Its green volume increased from $0.97m^3/m^2$ to $2.02m^3/m^2$, contributing to the improvement of the atmospheric environment and promotion of biodiversity. The density of deciduous broad-leaved trees at the top maintained at $18{\sim}21trees/100m^2$. In the areas where the understory vegetation was removed, Pueraria lobata spread as an invasive plant and disturbed the forest. In the areas where the understory vegetation was not removed, various native plant species including the Rhus javanica and Celtis sinensis were introduced and contributed to the development of a forest in a multiple structure. There was a concern with the spread of Robinia pseudoacacia which developed into understory vegetation in Pinus thunbergii forest and some canopy forest. The study proposed vegetation management directions for the back, top and front side according to the physical structure of large-scale buffer green zone formed in a long linear form and the usage characteristics of adjacent land.

• 한국환경생태학회지
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• 제26권2호
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• pp.210-218
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• 2012

본 연구는 자란개체군을 식물사회학적 방법으로 분류하고, 식생과 환경과의 상관관계를 밝히고자 분포서열법을사용하여 분석하였다. 자란개체군은 노간주나무 우점개체군, 산철쭉 우점개체군, 붉나무 우점개체군, 자란 전형개체군으로 분류되었다. 자란개체군은 주로 한반도의 남서해안을 따라 분포하고 해발고 4-40m의 해안가 낮은 사면의 평균 $41.7^{\circ}$의 다소 급한 경사지에 위치하고 있다. 토양의 이화학적 성분을 분석한 결과 유기물함량은 2.38-6.70%, 전질소함량 0.09-0.27%, 치환성 K 1.08-1.72$cmol^+/kg$, 치환성 Ca 3.56-7.71$cmol^+/kg$, 치환성 Mg 1.52-3.21$cmol^+/kg$, 양이온치환용량 5.28-16.95$cmol^+/kg$이며, 토양 pH는 4.60-6.01인 것으로 조사되었다. 산철쭉 우점개체군은 경사가 다소 급하고 토양내 양이온치환용량과 pH, 전질소, 유기물함량이 가장 높은 지역에 분포하였다. 붉나무 우점개체군은 경사가 다소 완만하고 양이온치환용량, pH, 전질소, 유기물함량이 상대적으로 낮은 지역에 분포하는 것으로 나타났다. 그리고 노간 주나무 우점개체군과 자란 전형개체군은 중간입지에 분포하는 것으로 나타났다. IUCN의 평가기준에 따라 취약종으로 지정된 희귀식물인 자란은 자생지가 해안가를 따라 분포하는 개체군의 생육특성 상 개발로 인한 훼손으로부터 자생지 의 보전을 위한 구체적인 대책 마련이 요구된다.

• 생명과학회지
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• 제16권2호
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• pp.328-332
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• 2006

Pancreatic lipase의 활성을 저해하는 물질을 탐색하고자 국내의 식용 또는 약용식물의 추출물을 대상으로 lipase 활성에 대한 저해능력이 있는 식물추출물은 탐색한 결과 대복피, 계혈등, 오배자 및 백자인의 식물추출물을 선정하였다. Pancreatic lipase에 대한 저해활성이 우수한 백자인으로부터 클로로포름 용매추출, 실리카겔 컬럼크로마토그라피, 세파덱스 LH-20 컬럼크로마토그라피와 고속액체크로마토그라피를 실시하여 pancreatic lipase에 대한 저해활성 물질로 TF-1, TF-2, TF-3를 분리하였다. 이들을 대상으로 porcine pancreatic lipase에 대한 저해효과를 측정한 결과, 활성물질 TF-1, TF-2, TF-3 및 orlistat의 porcine pancreatic lipase에 대한 $IC_{50}$ 값은 각각 44.7, 98.7, 46.1 및 $27.6{\mu}g/ml$인 것으로 나타났다. 활성물질 TF-2와 orlistat의 경우에는 $10{\mu}g/ml$의 농도에서 NIH-3T3 L1의 지방세포로의 분화에도 억제효과가 있음을 입증하였다.