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Inhibitory Effect of Thujae orientalis Semen Extract on Pancreatic Lipase Activity

백자인 추출물에 의한 pancreatic lipase의 저해 효과

  • Kim Min-Soo (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim Bo-Yeon (Korea Research Institute of Bioscience and Biotechnology) ;
  • Park Chan-Sun (Korea Research Institute of Bioscience and Biotechnology) ;
  • Yoon Byung-Dae (Korea Research Institute of Bioscience and Biotechnology) ;
  • Ahn Soon-Cheol (Department of Microbiology and Immunology, College of Medicine, Pusan National University) ;
  • Oh Won-Keun (Korea Research Institute of Bioscience and Biotechnology) ;
  • Ahn Jong-Seog (Korea Research Institute of Bioscience and Biotechnology)
  • Published : 2006.04.01

Abstract

The possible presence of inhibitors of pancreatic lipase (tricaylglycerol acylhydrolase EC 3.1.1.3) was screened from Korean traditional edible or medicinal herbs. Among tested herbs, Arecae pericarpium, Mucunae Caulis, Rhus javanica, Thujae orientalis were shown to have strong inhibitory effect against pancreatic lipase. Thujae orientalis was finally selected as a candidate for pancreatic lipase inhibitor. The extract of Thujae orientalis was showed selective inhibition on porcine pancreatic lipase activity. Active inhibitors, TF-1, TF-2, TF-3, were purified from an extract of Thujae orientalis, using chloroform extraction, followed by successive chromatography in silica gel and LH-20 and high performance liquid chromatography (HPLC). The $IC_{50}$ values of TF-1, TF-2, TF-3 and orlistat were 44.7, 98.7, 46.1 and $27.6{\mu}g/ml$, respectively. And also the TF-2 and orlistat were shown to be inhibitory effect on the differentiation of preadipocyte NIH-3T3 L1 cells at a concentration of $10{\mu}g/ml$.

Pancreatic lipase의 활성을 저해하는 물질을 탐색하고자 국내의 식용 또는 약용식물의 추출물을 대상으로 lipase 활성에 대한 저해능력이 있는 식물추출물은 탐색한 결과 대복피, 계혈등, 오배자 및 백자인의 식물추출물을 선정하였다. Pancreatic lipase에 대한 저해활성이 우수한 백자인으로부터 클로로포름 용매추출, 실리카겔 컬럼크로마토그라피, 세파덱스 LH-20 컬럼크로마토그라피와 고속액체크로마토그라피를 실시하여 pancreatic lipase에 대한 저해활성 물질로 TF-1, TF-2, TF-3를 분리하였다. 이들을 대상으로 porcine pancreatic lipase에 대한 저해효과를 측정한 결과, 활성물질 TF-1, TF-2, TF-3 및 orlistat의 porcine pancreatic lipase에 대한 $IC_{50}$ 값은 각각 44.7, 98.7, 46.1 및 $27.6{\mu}g/ml$인 것으로 나타났다. 활성물질 TF-2와 orlistat의 경우에는 $10{\mu}g/ml$의 농도에서 NIH-3T3 L1의 지방세포로의 분화에도 억제효과가 있음을 입증하였다.

References

  1. 보건복지부. 2002. 2001년 국민영양조사
  2. Alberti, K. G. and P. Z. Zimmet. 1988. Definition, diagnosis and classification of diabetes mellitus and its complications. Part 1: diagnosis and classification of diabetes mellitus provisional report of a WHO consultation. Diabet. Med. 13, 539-353
  3. Bitou, N., M. Nimomiya, T. Tsjita and H. Okuda. 1999. Screening of lipase inhibitors from marine algae. Lipids 34, 441-445 https://doi.org/10.1007/s11745-999-0383-7
  4. Borgstom, B. 1986. Luminal digestion of fat. pp. 361-373. In Go I(ed.). The exocrine pancreas : biology, pathobiology, and disease. Raven Press, New York
  5. Bray, G. A. and B. M. Popkin. 1998. Dietary fat intake dose affect obesity. Am. J. Clin. Nutr. 68, 1157-1173 https://doi.org/10.1093/ajcn/68.6.1157
  6. Bray, G. A. and B. M. Popkin. 1999. Dietary fat affects obesity rate. Am. J. Clin. Nutr. 70, 572-573 https://doi.org/10.1093/ajcn/70.4.572
  7. Diehl, A. K. 1991. Epidemiology and natural history of gallstone disease. Gastroenterol. Clin. N. Am. 20, 1-19
  8. Drent, M. L., I. Larsson, T. William-Olsson, F. Quaade, F. Czubayko, K. Von Bergmann, W. Strobel, L. Sjotro and E. A. Van der Veen. 1995. Orlistat (RO 18-0647), a lipase inhibitor, in the treatment of human obesity: a multiple dose study. Int. J. Obes. 19, 221 -226
  9. Flegal, K. M., M. D. Carroll, C. L. Ogden, and C. L. Johnson. 2002. Prevalence and trends in obesity among U.S. adults, 1999-2000. JAMA. 288, 1723-1727 https://doi.org/10.1001/jama.288.14.1723
  10. Freedman, D. S., M. K. Serdula, C. A. Perey and L. Whitle. 1997. Obesity levels of lipids and glucose, and smoking among Navajo adolescents. J. Nutr. 127, 2120S-2127S
  11. Grundy, S. M. 1999. Hypertriglyceridemia, insulin resistance, and the metabolic syndrome. Am. J. Cardiol. 83, 25F -29F https://doi.org/10.1016/S0002-9149(99)00211-8
  12. Hadvay, P., H. Lengsfeld and H. Wolter. 1988. Inhibition of pancreatic lipase in vitro by covalent inhibitor tetrahydrolipstatin. Biochem. J. 256, 357-361 https://doi.org/10.1042/bj2560357
  13. Hauptman, J. B., F. S. Jeunet and D. Hartmann. 1992. Initial studies in humans with the novel gastrointestinal lipase inhibitor Ro 18-0647(tetrahydrolipstatin). Am. J. Clin. Nutr. 55, 309S -313S https://doi.org/10.1093/ajcn/55.1.309s
  14. Larsson, B., P. Bjorntorp and G. Tibblin. 1981. The health consequences of moderate obesity. Int. J. Obes. Relat. Metab. Disord. 5, 97-106
  15. Peter, C. and G. Williams. 2001. Drug treatment of obesity: from past failures to future successes?. Br. J. Clin. Pharmacol. 51, 135-141
  16. Rexrode, K. M., J. E. Manson and C. H. Hennekens. 1996. Obesity and cardiovascular disease. Curr. Opin. Cardiol. 11, 490-495 https://doi.org/10.1097/00001573-199609000-00007
  17. Shimura, S., W. Tsuzuki, Y. Itho S. kobayashi and T. Suzuki. 1994. Inhibitory effect of tannin fraction Cassia mimosoides L. var. nomame makino on lipase activity. Nippon Shokuhin Kogyo Gakkaishi. 41, 561-564 https://doi.org/10.3136/nskkk1962.41.561
  18. Sjostrom, L., A. Rissanen, T. Andersen, M. Boldrin, A. Golay and H. P. F. Koppeschaar. 1998. Randomised placebo- controlled trial of orlistat for weight loss and prevention of weight regain in obese patients. Lancet 352, 167 -172 https://doi.org/10.1016/S0140-6736(97)11509-4
  19. Taylor, C. B., S. P. Fortman, J. Flora, S. Kayman, D. C. Barrett, D. Jatulis and J. W. Farghar. 1991. Effect of long term community health education on body mass index. Am. J. Epidemiol. 134, 235-249 https://doi.org/10.1093/oxfordjournals.aje.a116077
  20. Wada, K. and Y. Ikeda. 1998. Longitudinal studies to determine the effect of body fat rate reduction on blood pressure. J. Med. Syst. 22, 19-25 https://doi.org/10.1023/A:1022698221039
  21. World Health Organization Consultation on Obesity. 1998. Obesity: Preventing and Managing the Global Epidemic. World Health Organization: Geneva
  22. Yamamoto, M., S. Shimura, Y. Iyoh, M. Egawa and S. Ionue. 2000. Anti-obesity effects of lipase inhibitor CT-II, an extract from edible herbs, Nomame Herba, on rats fed a high-fat diet. Int. J. Obesity 24, 758-764 https://doi.org/10.1038/sj.ijo.0801222
  23. Zapf, J., E. W. Schoenole, M. Waldvogel, M. Sand and E. R. Froesch. 1981. Effect of trypsin treatment of rat adipocyte on biological effects and binding of insulin and insulin- like growth factors : Further evidence for the action of insulin-like growth factors through the insulin receptor. Eur. J. Biochem. 133, 605-609

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