• Asian-Australasian Journal of Animal Sciences
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• 제20권5호
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• pp.701-705
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• 2007

Seven rc mutant and seven normal male birds (Rhode Island Red suie, RIR) were used in this study to determine the effects of rc mutation on semen characteristics, testosterone profile and spermatogenic tissues. All birds were randomly selected at week 12 of age and housed in individual cages and were fed and watered ad libitum. The birds were exposed to a 14L:10D light cycle during experiment. Semen were collected at weeks 22 to 23 from each bird twice a week and evaluated for semen volume (SV), sperm concentration (SC), total sperm count (TSC), percent of sperm motility (%SM), dead sperm (%DS), and sperm metabolic activity (SMA). To determine the testosterone concentration (TC) in plasma, blood was collected at weeks 12, 16 and 18. Testicular tissue were collected, processed and evaluated for semineferous tubule diameter (STD), round spermatid number (RSN), percent elongated sperm (%ES) and semineferous tubules length (STL). Body weight (BW), comb weight (CW) and testes weight (TW) were weighted at the end of experiment (week 23). The SV, TSC and %SM were significantly higher in normal birds but the %DS was higher in blind birds (p<0.05). The SC did not differ significantly between the two groups but its value was higher in normal birds. The sperm metabolic activity in the first h of collection did not differ significantly between the two groups but after 24 h, the level of SMA in normal group was significantly higher (p<0.05). The level of TC did not differ significantly between the two genotype groups but normal birds had higher TC in all collections except the last one. The STD, RSN, %ES and STL in normal birds were higher when compared to blind birds but the differences were insignificant except for ES percent. The BW, CW and TW between the two groups did not differ significantly but the weights were higher in normal group compared to blind birds. Statistical analysis of semen characteristics, testosterone profile and histological factors were indicated detrimental effects of rc mutation in prepubertal RIR blind male birds due to lack of light.

• Korean Journal of Poultry Science
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• 제23권3호
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• pp.135-144
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• 1996

This study was carried out to clarify the genetic constitution of biochemical polymorphic loci controlling blood protein and enzymes as genetic rnarkers in Korean native chicken(KNG) population Blood samples were collected from 230 KNG representing three colored-lines(reddish-, yellowish- and blackish- brown) raised in Daejeon branch of National Livestock Research Institute. Eight blood marker loci, transferrin(Tf), post-albumin(Pas), albumin(Alb), amylase-1(Arny-1), es-terase-1(Es-1), alkaline phosphatase(Akp), catalase(Cat) and hemoglobin(Hh) were analyzed by using starch, agarose and polyacrylamide gel electrophoresis. Based on the gene frequencies of polymorphic marker loci, the genetic characteristics of KNF population was analyzed, and the genetic ariability within population was quantified. The genetic relationships between KNC and other native fowls or improved breeds were also estimated. The gene frequencies of Tf, Pas and AIb loci were similar to those of improved breeds among the seven biochemical polymorphic loci, while gene frequencies of Cat and Es-i loci were remarkably different between KNC and improved breeds. Gene frequencies of amy-i and Akp loci were similar to those of New Hampshire and Rhode Island Red and White Leghorn, respectively. However in comparison with other improved breeds, great differences were observed in gene frequencies of these loci The average heterozygosity, effective number of alleles and homogeneity index for the seven loci combined were estimated to be .334, 1.639 and .373, respectively. Based on the dendrogram and genetic distances, the KNC was genetically closer to New Hampshire, Plymouth Rock and Rhode Island Red breeds than to the White Leghorn breed.

• Asian-Australasian Journal of Animal Sciences
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• 제12권7호
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• pp.1085-1089
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• 1999

Energy requirement of Rhode Island Red (RIR) hens was studied by comparative slaughter technique. Seventeen hens above 72 weeks of age were slaughtered in batches. Batch I consisted of 5 hens which were slaughtered initially. Batch II comprised of six hens, which were fed ad libitum broken rice (BR)-based diet for 18 days. Record of feed intake, number of eggs laid and egg weight during the period was kept. These hens were slaughtered and body energy content was determined. Egg energy was consisted as energy deposited. Batch III consisting of six hens which were fed varying quantity of diet for 15 days, were slaughtered similarly as hens of batch II. Regression equation (body weight to body energy) developed on batch I was applied to batch II and developed on batch II was applied to batch III hens, to find out initial body energy content of hens. Egg energy (EE) was calculated according to formula: EE (kcal) = -19.7 + 1.81 egg weight (g). Regressing metabolisable energy (ME) intake on energy balance (body energy change + egg energy), maintenance ME requirement of hens was found to be $119.8kcal/kg\;W^{0.75}/d$. Multiple regression of ME required for production on energy retained as protein and fat (body plus egg energy) indicated that RIR hens synthesize proteins with an efficiency of 85.5 and fat with an efficiency exceeding 100 percent on BR based diet.

• Food Science of Animal Resources
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• 제38권3호
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• pp.468-475
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• 2018

The present trial was carried out to study the effect of sea buckthorn seed supplementation on egg quality of laying birds. A total of 160 Rhode Island Red${\times}$Fayoumi layers was divided into four groups of 40 birds each, which was further replicated four times with 10 hens per replicate. Group one was kept as a control, while other three groups were supplemented with sea buckthorn seed powder at a dose rate of 1 (T1), 2 (T2) and 3 (T3) g/kg of feed. The results showed that egg production was significantly (p<0.05) higher in T3 at the end of the study. Egg weight was significantly (p<0.05) high in T2 and T3 during week 39 and 40. Egg yolk weight was significantly (p<0.05) in T3 compared to the control. Significantly (p<0.01) lower egg cholesterol was recorded in T2 and T3. From the results of the present study, we concluded that laying hens supplemented with sea buckthorn at the rate of 2 and 3 g/kg improved the egg quality parameters and egg cholesterol.

• Korean Journal of Poultry Science
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• 제49권1호
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• pp.15-23
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• 2022

We compared the degrees of vitality of 12 Korean native chicken breeds, such as Jaeraejong, Korean Rhode Island Red (Rhode), Korean White Leghorn, Korean Cornish (Cornish), and Korean Ogye breeds. The survival rate and telomere length were measured as vital markers. Telomere length was analyzed via quantitative fluorescence in situ hybridization method using the lymphocytes of 466 chickens. We found that the telomere length decreased linearly with increasing chronological age in all chicken breeds. Telomere length and telomere shortening rates (TSR) were significantly different among the chicken breeds after 20 weeks of age (P<0.01). Rhode had the longest telomere length and the lowest TSR, whereas Cornish had the shortest telomere length and the highest TSR. In terms of TSR, the telomere length of 50-week-old chickens was half of that of 8-week-old chickens. There was also a significant difference in survival rates among the breeds. Both Rhode and Korean Ogye had the highest survival rates, while Cornish had the lowest. There was a significant positive correlation between survival rate and telomere length, and telomere length in old age showed a higher correlation with survival rate than that in young age. Therefore, it is considered that TSR is more closely related to survival rate than the telomere length. Based on the telomere dynamics and survival rates of 12 Korean native chicken breeds, it was concluded that the Rhode breed and Cornish breed had the highest and lowest vitality, respectively.

• Asian-Australasian Journal of Animal Sciences
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• 제22권9호
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• pp.1241-1247
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• 2009

A large number of branded chicken products exist in Japan, and in some cases, the breed of chicken is an important factor used to attract consumer interest in the retail product. In order to establish a simple method for verifying such breed claims we applied the amplified fragment length polymorphism (AFLP) technique to nine chicken breeds (White Cornish, Red Cornish, White Plymouth Rock, New Hampshire, Rhode Island Red, Barred Plymouth Rock, Hinaidori, Tosajidori, Tsushimajidori) to search for molecular markers able to discriminate chicken breeds. Three breed-specific single nucleotide polymorphisms (SNP) were identified, one for each of Hinaidori, Tosajidori, or New Hampshire. A total of 219 individuals from the nine breeds were analyzed using a specific PCR test for each of these SNP. The PCR tests made it possible to discriminate between the breeds of chickens to identify products from these three breeds. This PCR method provides an efficient method for the routine analysis and verification of certified chicken products.

• Korean Journal of Poultry Science
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• 제23권4호
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• pp.177-183
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• 1996

This study was conducted to analyze genetic characteristics of Korean Native Chicken three lines classified on the basis of the feather color and appearance (Red, Yellow, and Black) using DNA fingerprinting method. To estimate the genetic relatedness among breeds and similarities within breeds, we collected blood samples from Korean Native Chicken (KNC), Rhode Island Red (RIR), White Leghorn (WL), and Cornish(CN) and obtained genomic DNA from the blood of 10 individuals randomly selected within the breeds and lines. The genomic DNA samples were digested with restriction enzymes (Hinf J, Hae Ill) and hybridized with various probes (Jeffreys' probes 33.15, 33.6 and M13) after Southern transfer. Genetic similarities within breeds were characterized by band sharing (BS) value, estimated by the DFP band pattern between the pair of lanes. BS values within WL, RIR, and KNC were 0.82, 0.70 and 0.56, respectively. Relative genetic diversity (BS value) of KNC was higher than those two breeds (WL, RIR). Estimation of genetic similarity between KNC lines and control breed (RIR) was 0.32, whereas similarity within KNC lines (6 groups) was 0.50. In this analysis, KNC was showed to have a highly genetic diver-sity at the DNA level, and to be closer in genetic distance to RIR (0.67) than any other breeds.

• Korean Journal of Poultry Science
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• 제47권4호
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• pp.237-245
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• 2020

This study investigated the effects of breed, laying age, and egg storage period on hatching performance. Eggs were collected from early laying period (27 to 29 weeks old) and late laying period (50 to 52 weeks old) in Hwanggalsaek Jaeraejong, Korean Rhode Island Red, and Korean White Leghorn chicken breeds. Eggs laid for each period were stored for 3, 7 and 14 days and then hatched. Fertility, hatchability, and embryonic mortality were observed to determine the hatching performance. The results showed that hatching performance was affected by breed, laying age, and egg storage period with independently or interactively. The Korean Rhode Island Red breed had the lowest hatching performance. Hatchability and early embryonic mortality rate increased in early laying period eggs compared to the late laying period, but the late embryonic mortality rate increased in late laying period eggs. Additionally, hatching performance decreased as the egg storage period increased. Using breed-specific hatching management techniques, avoiding late laying period eggs, and shortening the egg storage period to seven days after laying may improve the hatching performance.

• Asian-Australasian Journal of Animal Sciences
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• 제4권3호
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• pp.231-234
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• 1991

Transferrin polymorphisms and growth rate up to 12 weeks of age were observed in RIR. crossbreds and native chickens. $Tf^{BB}$ genotype was observed in all types of bird. $Tf^{BC}$ occurred only in crossbred and native chickens. Frequency of $Tf^B$ was the highest in all types. Mean body weights at 8- and 12- weeks of age were the highest in RIR ($292{\pm}37.77$ and $612{\pm}75.69g$ respectively) and the lowest in natives for $Tf^{BC}$ ($180{\pm}11.54$ and $308{\pm}4.94g$ respectively). The difference in 12 week body weigh t of crossbreds between $Tf^{BB}$ and $Tf^{BC}$ was found to be significant (p<0.05). The results indicate that crossbreds ($Tf^{BB}$) may be grown effectively upto 12 weeks of age under Bangladesh conditions.

• Korean Journal of Poultry Science
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• 제25권1호
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• pp.21-30
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• 1998

The effects of dietary levels and feeding period of Korean hot pepper (Capsicum annum) seed on the performance and egg quality were investigated. Rhode Island Red layers of 84 wk of age were fed the experimental diets containing O.O(Control), 0.5, 1.0, 2.0, and 3.0% of HPS, respectively, in individual cages during 10 wk of the feeding period. The dietary hot pepper seed significantly(P<0.05) improved hen-day egg production and daily egg rnass, but reduced egg weight(P<0.05). During the 10 wk of the feeding period, the egg production and daily egg mass improved after 6 or 7 wk of feeding 0.5, 1.0 or 2.0% hot pepper seed diets. When the layers were fed the 3.0% HPS diet, it only took 1 wk to improve the egg production and daily egg mass. In addition, the birds fed 3.0% hot pepper seed diet showed relatively constant egg weight while those of the Control increased gradually as the feeding period passed. The dietary hot pepper seed significantly improved the eggshell thickness, and redness and whole color of egg yolk. The results indicate that dietary hot pepper seed( $\geq$2.0%) may improve the egg production and egg shell thickness, and increase the redness of egg yolk in layers.