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Radioprotective Effect of Quercetin Post-Treatment against γ-Irradiation-Induced Hepatocellular and Hematopoiectic System Damage in Mice (감마선 조사로 유도된 간세포와 조혈계 손상 마우스에서 퀘르세틴 투여 후의 방사선방호 효과)

  • Kang, Jung Ae;Yoon, Seon Hye;Rho, Jong Kook;Choi, Dae Seong;Jang, Beom-Su;Park, Sang Hyun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.44 no.7
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    • pp.970-974
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    • 2015
  • This study was designed to evaluate the therapeutic effect of quercetin against radiation-induced hepatocellular and hematopoiectic damage in BALB/c mice. Mice were exposed to 6 Gy of ${\gamma}$-radiation and orally administered quercetin (25, 50 mg/kg b.w.) for 7 consecutive days. ${\gamma}$-Irradiation caused marked elevation of serum aspartate aminotransferase and alanine aminotransferase, levels as well as reduction of spleen index, thymus index, and the number of white blood cells. In addition, ${\gamma}$-irradiation induced significant elevation of lipid peroxidation as well as reduction of antioxidant enzyme activities, including superoxide dismutase, catalase, and glutathione peroxidase. However, post-treatment with quercetin resulted in a significant recovery of all of these parameters. These results suggest that quercetin acts as a potent radioprotector against irradiation-induced cellular damage in mice.

Production of High-Level Polyphenol Powders from Young Grape Leaves (어린 포도 잎을 이용한 폴리페놀 고함유 분말 제조)

  • Chang, Seog-Won;Shin, Nam-Sub;Song, Jung-Hee;Kim, Hyun-Ju;Lee, Ki-Yeol;Rho, Yong-Taek
    • Food Science and Preservation
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    • v.16 no.5
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    • pp.714-718
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    • 2009
  • Polyphenols are naturally occurring antioxidants and play many important roles in human health. Grape leaves have not yet been reported to contain the antioxidants. In this study, the physicochemical properties of powders from young grape leaves, obtained after hot-air or vacuum freeze drying, were analyzed. Weight loss rangedfrom 76-80%, but no difference was found between samples prepared by hot-air orvacuum freeze drying. The L-, a-, and b- values of grape powder all fell upon hot-air drying. Quercetin, resveratrol, catechin, and epicatechin were detected in all cultivars. The contents of the four polyphenols were generally higher in powders obtained by vacuum freeze dryingthan in those prepared by hot-air drying, indicating that vacuum freeze drying is better for optimal preservation of polyphenols. None of three polyphenols assayed was found in some cultivars or after certain drying treatments. Based on physiochemical properties, our findings suggest that powders obtained after vacuum freeze drying are optimal for industrial use.

A Characteristic Study on Shear Strength of Reinforced Concrete Beams according to Shear Reinforcement Ratio and Beam Section Size (전단철근비와 보의 단면크기에 따른 철근콘크리트 보의 전단강도 특성 연구)

  • Noh, Hyung-Jin;Yu, In-Geun;Lee, Ho-Kyung;Baek, Seung-Min;Kim, Woo-Suk;Kwak, Yoon-Keun
    • Journal of the Architectural Institute of Korea Structure & Construction
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    • v.35 no.6
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    • pp.111-119
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    • 2019
  • The purpose of this study is to investigate the shear strength of reinforced concrete beam according to beam section size and shear reinforcement ratio. A total of nine specimens were tested and designed concrete compressive strength is 24 MPa. The main variables are shear reinforcement ratio and beam section size fixed with shear span to depth ratio (a/d = 2.5), the tensile reinforcement ratio (${\rho}=0.013$) and width to depth ratio (h/b = 1.5). The test specimens were divided into three series of S1 ($225{\times}338mm$), S2 ($270{\times}405mm$) and S3 ($315{\times}473mm$), respectively. The experimental results show that all specimens represent diagonal tensile failure. For $S^*-1$ specimens (d/s=0), the shear strength decreased by 33% and 46% with increasing the beam effective depth, 26% and 33% for $S^*-2$ specimens (d/s=1.5) and 16% and 20% for $S^*-3$ specimens (d/s=2.0) respectively. As the shear reinforcement ratio increases, the decrease range in shear strength decreases. In other words, this means that as the shear reinforcement ratio increases, the size effect of concrete decreases. In the S1 series, the shear strength increased by 39% and 41% as the shear reinforcement ratio increased, 54% and 76% in the S2 series and 66% and 100% in the S3 series, respectively. As the effective depth of beam increases, the increase range of shear strength increases. This means that the effect of shear reinforcement increases as the beam effective depth increases. As a result of comparing experimental values with theoretical values by standard equation and proposed equation, the ratio by Zsutty and Bazant's equation is 1.30 ~ 1.36 and the ratio by KBC1 and KBC2 is 1.55~.163, respectively. Therefore, Zsutty and Bazant's proposed equation is more likely to reflect the experimental data. The current standard for shear reinforcement ratio (i.e., $S_{max}=d/2$) is expected to be somewhat relaxed because the ratio of experimental values to theoretical values was found to be 1.01 ~ 1.44 for most specimens.

A Study on Economic Evaluation Modeling of MVDC Distribution System for Hosting Capacity of PV System (태양광전원 수용을 위한 MVDC 배전망의 경제성평가 모델링에 관한 연구)

  • Lee, Hu-Dong;Kim, Ki-Young;Kim, Mi-Sung;Rho, Dae-Seok
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.22 no.3
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    • pp.1-12
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    • 2021
  • Technologies for an MVDC(medium voltage direct current) distribution system are being considered as an effective alternative to overcome the interconnection delay issues of PV systems. However, the implementation of a DC distribution system might lead to economic problems because of the development of DC devices. Therefore, this paper deals with the scale of a PV plant based on its capacity and proposes hosting-capacity models for PV systems to establish a network to evaluate the feasibility of an MVDC distribution system. The proposed models can be classified as AC and DC distribution systems by the power-supply method. PV systems with hundreds of MW, dozens of MW, and a few MW can be categorized as large-scale, medium-scale, and small-scale models, respectively. This paper also performed modeling for an economic evaluation of MVDC distribution system by considering both the cost of AC and DC network construction, converter replacement, operation, etc. The profit was composed of the SMP and REC rate of a PV plant. A simulation for economic evaluation was done for the MVDC distribution system using the present worth and equal-principal costs repayment method. The results confirmed that the proposed model is a useful tool to evaluate economic issues of a DC distribution system.

Analysis of Structural Relationship between Science Academic Achievement, Learning Support from Teachers, Students' Attitude toward Science, and School Life from TIMSS 2019, and National Assessment of Educational Achievement (TIMSS 2019와 국가수준 학업성취도 평가에 나타난 과학성취도와 교사의 학습 지원, 과학에 대한 태도, 학교 생활의 구조적 관계 비교 분석)

  • Rho, Jaehee;Ryoo, Ji Hoon
    • Journal of The Korean Association For Science Education
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    • v.42 no.1
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    • pp.149-160
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    • 2022
  • Comparative studies using large-scale data such as TIMSS, PIRLS, and PISA inform us of the effectiveness of each educational system. Even though samples in the large-scale studies were representative, admitting potential discrepancy when applying the findings of the large-scale studies to local educational system is still needed. This study examines the structural relationship among students' attitude towards science, learning support from teachers, school life, and science academic achievement with both large-scale data and local comparative study data utilizing same variables. Responses on the TIMSS 2019 of 5,554 Korean seventh-grade students and National Assessment of Educational Achievement (NAEA) 2019 of 6,365 third-grade middle school students were used. The results indicate that: a) school life did not affect the science achievements in both data. However, in NAEA 2019, students' attitude mediated the relationship between school life and science achievement; b) learning support from teachers had a significant impact on TIMSS science achievements, and also had positive effect on achievement through students' attitude in TIMSS. On the other hand, learning support had a positive effect on achievement only when student's attitude mediated the relationship in NAEA; c) students 'attitude toward science had positive effect on science achievement on both data; d) the impact of gender was different on school life, academic achievement, students 'attitude towards science, and learning support from teachers on both data; and e) the impact of the number of books differed as well. There were differences in results between the international and domestic research, which inform us that we need to pay attention when interpreting the domestic environment through the results of international research.

PPIA, HPRT1, and YWHAZ are suitable reference genes for quantitative polymerase chain reaction assay of the hypothalamic-pituitary-gonadal axis in sows

  • Kim, Hwan-Deuk;Jo, Chan-Hee;Choe, Yong-Ho;Lee, Hyeon-Jeong;Jang, Min;Bae, Seul-Gi;Yun, Sung-Ho;Lee, Sung-Lim;Rho, Gyu-Jin;Kim, Seung-Joon;Lee, Won-Jae
    • Animal Bioscience
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    • v.35 no.12
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    • pp.1850-1859
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    • 2022
  • Objective: The quantitative reverse transcription polymerase chain reaction (qPCR) is the most accurate and reliable technique for analysis of gene expression. Endogenous reference genes (RGs) have been used to normalize qPCR data, although their expression may vary in different tissues and experimental conditions. Verification of the stability of RGs in selected samples is a prerequisite for reliable results. Therefore, we attempted to identify the most stable RGs in the hypothalamic-pituitary-gonadal (HPG) axis in sows. Methods: The cycle threshold values of nine commonly used RGs (18S, HPRT1, GAPDH, RPL4, PPIA, B2M, YWHAZ, ACTB, and SDHA) from HPG axis-related tissues in the domestic sows in the different stages of estrus cycle were analyzed using two RG-finding programs, geNorm and Normfinder, to rank the stability of the pool of RGs. In addition, the effect of the most and least stable RGs was examined by normalization of the target gene, gonadotropin-releasing hormone (GnRH), in the hypothalamus. Results: PPIA, HPRT1, and YWHAZ were the most stable RGs in the HPG axis-related tissues in sows regardless of the stages of estrus cycle. In contrast, traditional RGs, including 18S and ACTB, were found to be the least stable under these experimental conditions. In particular, in the normalization of GnRH expression in the hypothalamus against several stable RGs, PPIA, HPRT1, and YWHAZ, could generate significant (p<0.05) elevation of GnRH in the preovulatory phase compared to the luteal phase, but the traditional RGs with the least stability (18S and ACTB) did not show a significant difference between groups. Conclusion: These results indicate the importance of verifying RG stability prior to commencing research and may contribute to experimental design in the field of animal reproductive physiology as reference data.

An Analysis of the Landscape Cognitive Characteristics of 'Gugok Streams' in the First Half of the 18th Century Based on the Comparison of China's 『Wuyi-Gugok Painting』 (중국 『무이구곡도』 3폭(幅)의 비교 분석을 통해 본 18세기 무이산 구곡계(九曲溪)의 경물 인지특성)

  • Cheng, Zhao-Xia;Rho, Jae-Hyun;Jiang, Cheng
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.37 no.3
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    • pp.62-82
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    • 2019
  • Taking the three Wuyi-Gugok Drawings, 『A Picture Showing the Boundary Between Mountains and Rivers: A』, 『Landscape of the Jiuqu River in the Wuyi Mountain: B』 and 『Eighteen Sceneries of Wuyi Mountain: C』, which were produced in the mid-Qing Dynasty as the research objects and after investigating the names recorded in the paintings, this paper tries to analyze the scenic spots, scene types and images in the literature survey. Also, based on the number of Scenic type and the number of Scenic name in each Gok, landscape richness(LR) and landscape similarity(LS) of the Gugok scenic spots, the cognitive characteristics of the landscape in the 18th century were carefully observed. The results are as follows. Firstly, according to the description statistics of scenic spot types in Wuyi Mountain Chronicle, there were 41 descriptions of scenery names in the three paintings, among which rock, peak and stone accounted for the majority. According to the data, the number of rocks, peaks and stones in Wuyi-Gugok landscape accounted for more than half, which reflected the characteristics of geological landscape such as Danxia landform in Wuyi-Gugok landscape. Secondly, the landscape of Gugok Stream(九曲溪) was diverse and full of images. The 1st Gok Daewangbong(大王峰) and Manjeongbong(幔亭峰), the 2nd Gok Oknyeobong(玉女峰), the 3rd Gok Sojangbong(小藏峰), the 4th Gok Daejangbong(大藏峰), the 5th Gok Daeeunbyeong(大隱屛) and Muijeongsa(武夷精舍), the 6th Gok Seonjangbong(仙掌峰) and Cheonyubong(天游峰) all had outstanding landscape in each Gok. However, the landscape features of the 7th~9th Gok were relatively low. Thirdly, according to the landscape image survey of each Gok, the image formation of Gugok cultural landscape originates from the specificity of the myths and legends related to Wuyi Mountain, and the landscape is highly well-known. Due to the specificity, the landscape recognition was very high. In particular, the 1st Gok and the 5th Gok closely related to the Taoist culture based on Muigun, the Stone Carving culture and the Boat Tour culture related to neo-confucianism culture of Zhu Xi. Fourthly, according to the analysis results of landscape similarity of 41 landscape types shown in the figure, the similarity of A and C was very high. The morphological description and the relationship of distant and near performance was very similar. Therefore, it could be judged that this was obviously influenced by one painting. As a whole, the names of the scenes depicted in the three paintings were formed at least in the first half of 18th century through a long history of inheritance, accumulated myths and legends, and the names of the scenes. The order of the scenery names in three Drawings had some differences. But among the scenery names appearing in all three Drawings, there were 21 stones, 20 rocks and 17 peaks. Stones, rocks and peaks guided the landscape of Gugok Streams in Wuyi Mountain. Fifthly, Seonjodae(仙釣臺) in A and C was described in the 4th Gok, but what deserved attention was that it was known as the scenery name of the 3rd Gok in Korean. In addition, Seungjindong(升眞洞) in the 1st Gok and Seokdangsa(石堂寺) in the 7th Gok were not described in Drawings A, B and C. This is a special point that needs to be studied in the future.

Epidemiologic Investigation for the Etiology of an Epidemic Ocurred among Animals and Humans in an Isolated Island, Korea(I) (신안군(新安郡) 낙도(落島)에서 발생(發生)한 괴질(怪疾)의 원인(原因)에 관한 역학적(疫學的) 조사(調査)(I))

  • Kim, J.S.;Heo, Y.;Yoon, H.Y.;Lee, W.Y.
    • Journal of Preventive Medicine and Public Health
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    • v.22 no.2 s.26
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    • pp.290-301
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    • 1989
  • This is preliminary report on anthrax epidemic occurred in an island with about 100 residents. Since 1982 there had been sudden deaths among all kinds of domestic animals including cattle, dogs, ducks, chicken and goat but only a few among cats in an isolated island about three hours distance away by ferry boat from Mokpo city. From 1986 through 1988 nine human deaths and four patients occurred, which made the government intervene for investigation on June 25 1988. The epidemiological investigation consisted of interview survey and medical examination, medical record analysis, laboratory work to isolate the pathogens under the direction of hypothesis derived from the study and further confirmation of the pathogens by international institute. The summarized results are as followings: 1. According to the interview survey there were many deaths among domestic animals usually in cold and dry season such as January through March and September through November; 36 heads of cattle leaving one head, more than 40 hogs(all), hundreds of chicken leaving few alive, goats that had taken home from mountain and two or three cats out of around 40 had sudden deaths from 1982 till 1985, when the residents stopped to purchase and take them into the island anymore. Also there were eleven persons who had experienced the similar syndrome complex to those of admitted and expired patients and four of them revealed typical chest X-ray findings; from one of these four patients(Rho) B. anthracis is isolated. 2. Medical record on patients who had been admitted, showed common characteristics of the disease course. On admission they had either gastrointestinal or upper respiratory infection symptoms which invariably progressed to septicemic nature with pulmonary interstitial infiltration and mediastinal widening/bulging, and then to deadly acute respiratory distress syndrome. At the end stage chest X-ray revealed multiple bullous emphysema. One of another characteristics was oral ulceration with bleeding occurred in about 50% of the patients. Laboratory test results in common were leukocytosis with left shift and abnormal liver and kidney functions, particularly at the later stage of the illness. 3. Epidemiological characteristics was striking in that both mortality and incidence rates were high: the mortality rate was 8.7% average, male being three times higher than females but there was no distictive clustering by age group. The incidence rate for both sexes was 28.2% and there was no sex difference although a tendency of higher incidence among older ages was noticed. The highest mortality and incidence were observed in Won village where the first death of animal occurred and with the highest frequency among three villages of the island. 4. Among twelve bacilli species isolated from various specimens, two strains, one from patient and the other from soil where the recently died cow is hurried, were confirmed as B. anthracis by Pasteur Institute and CDC of USA(strain from soil). CDC reported that the strain did not produce capsule in bicarbonate media but reacted with the bacteriophage and one of five sera taken from the patients. Mode of transmission as well as incubation period of the agent has not been established yet, which needs further investigation in relation to the antigenic structure of the variant when it is confirmed.

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A Study on Constituents of the New Apprenticeship Concept for the Promotion of Industrial Growth Potential (산업 성장잠재력 제고를 위한 신도제제도의 개념 요소에 대한 연구)

  • Yin, Zi Long;Rho, Tae Chun;Choi, Won Sik
    • 대한공업교육학회지
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    • v.38 no.1
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    • pp.1-27
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    • 2013
  • The purpose of this study was to find out the areas and their constitute elements of new apprenticeship through the expert of vocational education to improve the growth potential in the field of industry. Through the three times Delphi research process final composing areas and elements(total 6 areas and 41 sub-elements) of new apprenticeship were extracted. Followings are specific study results of 41 sub-elements for the 6 areas. In area A(Technology Skill aspect) total nine sub-elements were deducted as follows. Technology skill's field appling ability, new technology skill's acquisition, quality assurance ability, research development ability, material management using ability, problem solving ability, core technology skill understanding ability, idea's imagery expressing ability, creative design ability. In area B(Institutional aspect) total five sub-elements were deducted as follows. Flexible human material support, precise division of works, objective result assessment, institutionalization of responsibilities and liabilities between teacher and student, institutionalization of duty invention reward. In area C(Affective aspect) total eight sub-elements were deducted as follows. Manners and cooperation between teacher & student and peer, values for job, basic attitude for technology, job ethic sense, respect of other organization, active action to organization change, attitude of technology successor, service mind. In area D(Self-improvement aspect) total nine sub-elements were deducted as follows. Self evaluation and reflection, cultivate of organization understanding, career planning and developing ability, sound philosophy of life, communication ability, decision making ability, prepare of individual competence enhance system, self-control ability improvement, reaction of unexpected situation. In area E(Knowledge aspect) total four sub-elements were deducted as follows. Basic knowledge of relevant area, knowledge of new technology & preceding technology, fusion and relocation of knowledge, practical knowledge. In area F(Environmental aspect) total six sub-elements were deducted as follows. Awareness of business environment, understanding of education and practice environment, understanding of apprenticeship's business demand, connectivity of region community, adapt ability of labor market's change, awareness of society environment change.

Seedling Production of Rabbitfish, Siganus canaliculatus (흰점독가시치, Siganus canaliculatus의 종묘생산)

  • Hwang, Hyung-Kyu;Lee, Jung-Uie;Rho, Sum;Yang, Sang-Geun;Kim, Seong-Cheol;Kim, Kyong-Min
    • Journal of Aquaculture
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    • v.13 no.4
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    • pp.277-284
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    • 2000
  • Rabbitfish hatchlings were given a mixed food of rotifers (Brachionus rotundiformis) and trochophore larvae of oyster. Only the oyster-trochophore larvae were found in the gut of 62-h old fish larvae. The fish larvae, fed on rotifer and ciliate alone did not survive. However, their survival increased to 3.3 % on the 10th day after hatching, when trochophore was supplemented. Corresponding with the accelerated growth, the number of rotifers consumed increased from 11 in a 5-day old fish to 165 in a IS-day old fish. In a field ecosystem containing live diatom, Nannochloropsis oculata, rotifers and copepods, fish larvae were shocked and the oyster's trochophore larvae were fed from 2 to 7 days after hatching. A total of 76,000 seedling were produced after 50 days of hatching with 12.7 % survival. Mean total length and body weight were 65.6 mm and 3.4 g, respectively. Growth of body length (BL), body height (BH), body weight (BW) and head length (HL) as a function of the total length (TL) showed regressional relationships as follows; BL=0.8565 TL+0.0852 ($t^2$=0.9996); BH=0.3207 TL - 0.5052 (($t^2$=0.9641) BW=0.0652 TL2.3508 (($t^2$=0.9925); HL=0.2595 TL - 0.1898 (($t^2$=0.9901)

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