• Korean Journal of Plant Resources
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• v.28 no.6
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• pp.743-751
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• 2015

Reynoutria japonica and R. sachalinensis have been used as medicinal resources in Korea. However, it is difficult to identify and determine these medicinal herbs correctly because they are usually customized and purchased as the fragmented rhizomes types. To develop molecular markers for distinguishing two species, we analyzed and compared the chloroplast DNA sequences of seven loci (atpB, matK, ccD-psaI, atpF-H, trnL-trnF, psbK-I and rpl32-trnL). Among them, we found two effective SNPs in psbK-I region for R. japonica and atpF-H region for R. sachalinensis. Based on these SNP sites, we designed the new R. japonica- specific primer which is able to amplify 300 bp fragment in psbK-I region. A similar strategy was applied for the atpF-H region of R. sachalinensis. These molecular markers would be successfully applied to recognize R. japonica and R. sachalinensis.

• The Korean Journal of Mycology
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• v.24 no.4 s.79
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• pp.322-328
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• 1996

It is possible to cultivate Pleurotus ostreatus on the crush medium of Reynoutria sachalinensis. The crush of leaves and stems of Reynoutria sachalinensis with water (1:8 W/V) enhanced mycelial growth of P. ostreatus. That mycelial growth of P. ostreatus on the crush medium was accelerated three times as fast as that on malt extract agar (MEA), and mycelial compactness was denser than that of on MEA. The same result was obtained on mixture of saw dust and the crush of leaves and stems in test tube and bottle. The addition of rice bran and the crush to saw dust was best for mycelial growth. Regardless of pH (4.5, 6.5 and 8.5), P. ostreatus could suppress the growth.

• Korean Journal of Medicinal Crop Science
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• v.13 no.2
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• pp.80-84
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• 2005

Five known anthraquinones, physcion (1), I-O-methylemodin (2), emodin (3), $physcion-8-O-{\beta},-D-glucopyranoside$ (5), $emodin-8-O-{\beta},-D-glucopyranoside$ (6) and two known stilbenes, trans-resveratrol (4), $trans-resveratrol-3-O-{\beta},-D-glucopyranoside$ (7) were isolated from MeOH extract of Reynoutria sachalinensis (Polygonaceae). All structures were unambiguously established by 1D and 2D NMR and MS data and the compounds were evaluated for their cytotoxicity against L1210, HL-60, BI6F10 tumor cell lines in MTT assay. Among the compounds, trans-resveratrol (4) exhibited significant cytotoxic activity with $IC_{50}$ values of 9.2, 6.7 and $9.8\;{\mu}g/ml$, against the test cell lines respectively, but compounds 1-3 exhibited the moderate cytotoxic activity.

• Archives of Pharmacal Research
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• v.28 no.1
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• pp.22-27
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• 2005

Bioassay-guided fractionation of methanol extract of Reynoutria sachalinensis flower using DPPH assay has led to the isolation of three anthraquinones and three flavonoids. Their structures were identified as emodin (1), emodin-8-O-$\beta$-D-glucopyranoside (2), physcion-8-O-$\beta$-D­glucopyranoside (3), quercetin-3-O-$\alpha$-L-arabinofuranoside (4), quercetin-3-O-$\beta$-D-galactopyra­noside (5), and quercetin-3-O-$\beta$-D-glucuronopyranoside (6) by comparing their physicochemical and spectral data with those published in literatures. All isolated compounds were evaluated for antioxidant activities with free radical 1, 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging, superoxide radical scavenging and $Cu^{2+}$-mediated low density lipoprotein (LDL) oxidation assay. The results demonstrated that three flavonoids, 4, 5, and 6 had remarkable antioxidant activities with the $IC_{50}$ values of 64.3, 54.7, and 46.2${\mu}M$ (DPPH scavenging), the $IC_{50}$ values of 6.0, 6.7, and $4.4{\mu}M$ (superoxide radical scavenging) and the $IC_{50}$ values of 3.8, 3.2, and 5.4${\mu}M$ against LDL oxidation, respectively.

• Korean Journal of Plant Taxonomy
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• v.41 no.1
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• pp.51-57
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• 2011

The microstructure of the leaf epidermis and trichomes of Fallopia sect. Reynoutria are examined using scanning electron microscopy. Fallopia sachalinensis was distinguished from other taxa in this section by its prominent epicuticular wax layer consisting of protruding wax rodlets. In addition, epicuticular rodlets of F. sachalinensis individuals from Ullung Island and Dok Island appear to be thinner than those from other regions, including Japan and Sakhalin. The stomatal size appears to be related to the ploidy level in the sect. Reynoutria, as the hexaploids, octoploids and dodecaploids tend to have larger stomata as compared to tetraploids. Three basic types of trichomes were found in the section; (1) conical unicellular trichomes, (2) uniseriate filiform trichome consisting of 1-8 cells, and (3) peltate glandular trichomes. The trichome types and their distribution appear to be useful in distinguishing the taxa in the section.

• The Korean Journal of Mycology
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• v.47 no.3
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• pp.241-247
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• 2019

In 2017, we collected a wood-decay fungus growing on a dead and decaying herbaceous plant (Reynoutria sachalinensis (F. Schmidt) Nakai) in Dokdo, the far-eastern island of South Korea. Morphologically, this species is characterized by resupinate, coriaceous to corky basidiocarps, poroid hymenophores, pseudodimitic hyphal system, and ellipsoid basidiospores. Based on morphological observation and internal transcribed spacer of ribosomal DNA, the fungus was identified as Xylodon flaviporus (Berk. & M.A. Curtis ex Cooke) Riebesehl & Langer. It is only the second macrofungal species reported from Dokdo, and R. sachalinensis is the first herbaceous plant to be identified as a host for X. flaviporus.

• YAKHAK HOEJI
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• v.27 no.1
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• pp.37-43
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• 1983

The rhizome of Polygonum sachalinense Fr. Schm. (=Reynoutria sachalinensis Nakai, Polygonaceae) has been used as "Polygoni Rhizoma" (Hu Zhang) in the Orient as laxatives, diuretics and for treatment of suppurative dermatitis, gonorrhoea, favus and athlete's foot. From the methanolic extract of the dried rhizome physcion, emodin, emodin-8-O-$\beta$-D-glucoside as anthraquinone derivatives and .betha.-sitosterol glucoside were isolated and identified. Stilbene derivatives which have antibacterial and antifungal activities were also isolated.

• Korean Journal of Plant Taxonomy
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• v.41 no.1
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• pp.10-15
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• 2011

Fifteen populations comprising three taxa and a putative hybrid of Fallopia sect. Reynoutria in Korea were examined for their leaf flavonoid constituents. Nineteen flavonoid compounds were isolated and identified; they were glycosylated derivatives of the flavonols quercetin and kaempferol, and of the flavones apigenin and luteolin. Among them, quercetin 3-O-galactoside and quercetin 3-O-glucoside were major flavonoid constituents, and present in all taxa. The flavonoid data appear to be very useful for taxon delimitation, and all taxa examined are readily distinguished by their flavonoid profiles. In addition, the flavonoid data suggest that the Nonsan population may be of hybrid origin involving F. japonica var. japonica, F. forbesii, and F. sachalinensis. In F. japonica var. japonica, there is no apparent correlation between their levels of polyploidy and flavonoid chemistry, but geographical variation of the flavonoid profiles among some populations was detected.

• Korean Journal of Pharmacognosy
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• v.39 no.3
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• pp.223-227
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• 2008

Advanced glycation end products (AGEs) contribute to the progression of micro and macrovsacular complication of diabetes and therefore present a promising target for therapeutic agents. In this study, 40 Korean herbal medicines have been investigated with an in vitro evaluation system using AGEs inhibitory activity. Of these, 21 herbal medicines $(IC_{50}<50{\mu}g/ml)$ exhibited an inhibitory activity against AGEs formation compared with anminoguanidine $(IC_{50}=72.12{\mu}g/ml)$. Particularly, 7 herbal medicines, Actinidia arguta (root and stem), Crataegus pinnatifida (twig), Camellia japonica (whole), Kalopanax pictus (bark), Lagerstroemia indica (leaf-stem), Reynoutria sachalinensis (root) showed more potent inhibitory activity (approximately 3-10 fold) than the positive control aminoguanidine.

• Journal of Environmental Science International
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• v.16 no.6
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• pp.751-761
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• 2007

This study is carried out through ecological situation of native Bupleurum latissimum habitats and the research of environmental conditions for conservation of native sites, restoration of alternative habitats and preliminary data for application of useful medicinal plant. Native Bupleurum latissimum habitats were distributed on lower and middle part of the mountain from Altitude 54m to 185m, and the directions of slope were researched as usually northen and western slope. As a result of phytosociological research in 12 stands of native Bupleurum latissimum habitats, they were seperated to two communities(Bupleurum latissimum typical community and Bupleurum latissimum-Galium spurium community) in accordance with containable rate of the organic matter and soil hardeness. Soil condition is usually constituted as loamy sand and clay loam. Bupleurum latissimum is propagated in valuable soil conditions. Bupleurum latissimum lives with Morus bombycis, Reynoutria sachalinensis in Bupleurum latissimum typical community and Thalictrum aquilegifolium, Stellaria media, Hedera rhombea etc. is appeared with Bupleurum latissimum in Bupleurum latissimum-Galium spurium community. Especially, companion species were researched as Aster glehni, Dystaenia takeshimana and so on. The flora of vascular plants in this study is 44 families, 88genus 82species, 2 subspecies, 14 varietas, 4 forma and total 102 taxa. Plants of compositae occupied 10.78% among whole of them.