• Journal of Life Science
• /
• v.10 no.2
• /
• pp.210-217
• /
• 2000

A kinetic profile of the catalytic domain of stromelysin-1 (SCD) using the fluorescent peptide substrate has been determined by the stopped-flow technique. The pH profile has a pH optimum of about 5.5 with an extended shoulder above pH 7. Three pKa values, 5.0, 5.7, and 9.8 are found for the free enzyme state and two pH independent Kcat/Km values of 4.1$\times$104 M-1 s-1 and 1.4$\times$104 M-1 s-1 at low and high pH, respectively. The profile is quite different in shape with other MMP family which has been reported, having broad pH optimum with two pKa values. The substrate specificity of SCD towards fluorescent heptapeptide substrates has been also examined by thin layer chromatography. The cleavage sites of the substrates have been identified using reverse-phase HPLC method.SCD cleaves Dns-PLA↓L↓WAR and Dns-PLA↓L↓FAR at two positions. However, the Dns-PLA↓LRAR, Dns-PLE↓LFAR, adn Dns-PLSar↓LFAR are cleaved exclusively at one bond. The double cleavages of Dns-PLALWAR and Dns-PLALFAR by SCD are in marked contrast to the close structurally related matrilysin. A notable feature of SCD catalysis agrees with the structural data that the S1' pocket of SCD is deeper than that of matriysin. The differences observed between SCD and matrilysin may form the basis of understanding the structural relationships and substrate specificities of the MMP family in vivo.

• Journal of the Korean Orthopaedic Association
• /
• v.53 no.6
• /
• pp.505-512
• /
• 2018

Purpose: Many reconstruction methods have been attempted after an en-bloc resection of the proximal humerus. In particular, the introduction of reverse shoulder arthroplasty (RSA) has made a breakthrough in the functional recovery of the shoulder. Nevertheless, RSA has limitations when the humeral bone stock loss is significant. In addition, it is unclear if RSA is effective in patients showing failure with non-operative treatment of a proximal humeral tumor. Materials and Methods: A reconstruction was performed using an overlapping allograft-RSA composite for 11 patients with a failed proximal humeral construct. Delayed RSA was performed on 6 patients with failed non-operative treatment. The pre- and postoperative Musculoskeletal Tumor Society (MSTS) score and the complications were addressed. Results: Overlapping allograft-RSA composite afforded a stable construct in 11 failed proximal humeral reconstructions and the patient's chief complaints were resolved. The mean time to the union of overlapped allograft-host junction was 5.5 months. Average preoperative MSTS score of 20.3 point increased to 25.7 point, postoperatively. Four of the six patients who had RSA within 4 years from the index operation showed arm elevation of more than $90^{\circ}$ whereas the remaining 5 patients showed some disability. The complications include one case each of dislocation and aseptic infection, which were resolved by changing the polyethylene liner and scar revision, respectively. None of the 6 patients who underwent delayed RSA after the failure of non-operative treatment showed arm elevation more than $90^{\circ}$. Conclusion: An overlapping allograft-RSA composite is a simple and reliable reconstructive modality in patients with massive bone loss. In patients with metastatic cancer necessitating a surgical resection at presentation, early conversion to RSA is recommended to secure functional recovery.

• Asian-Australasian Journal of Animal Sciences
• /
• v.21 no.11
• /
• pp.1544-1550
• /
• 2008

The retinoids (vitamin A and its derivatives) play a critical role in vision, growth, reproduction, cell differentiation and embryonic development. Using the IMpRH panel, porcine cellular retinol binding protein genes 5 and 7 (RBP5 and RBP7) were assigned to porcine chromosomes 5 and 6, respectively. The complete coding sequences (CDS) of the RBP5 and RBP7 genes were amplified using the reverse transcriptase polymerase chain reaction (RT-PCR) method, and the deduced amino acid sequences of both genes were compared to human corresponding proteins. The mRNA distributions of the two genes in adult Wuzhishan pig tissues (lung, skeletal muscle, spleen, heart, stomach, large intestine, lymph node, small intestine, liver, brain, kidney and fat) were examined. A total of nine single nucleotide polymorphisms (SNPs) were identified in two genes. Three of these SNPs were analyzed using the polymerase chain reaction-restriction-fragment length polymorphism (PCR-RFLP) method in Laiwu, Wuzhishan, Guizhou, Bama, Tongcheng, Yorkshire and Landrace pig breeds. Association analysis of genotypes of these SNP loci with economic traits was done in our experimental populations. Significant associations of different genotypes of $RBP5-A/G^{63}$, $RBP5-A/G^{517}$ and $RPB5-T/C^{intron1-90}$ loci with traits including maximum carcass length (LM), minimum carcass length (LN), marbling score (MS), back fat thickness at shoulder (SBF), meat color score (MCS) and hematocrit (HCT) were detected. These SNPs may be useful as genetic markers in genetic improvement for porcine production.

• Asian-Australasian Journal of Animal Sciences
• /
• v.19 no.9
• /
• pp.1257-1261
• /
• 2006

Pleiomorphic adenoma gene-like1 (PLAGL1) encodes a zinc-finger (ZF) protein with seven ZFs of the C2H2-type which is a regulator of apoptosis and cell cycle arrest, and also regulates the secretion of insulin. In both human and mouse, PLAGL1 is a candidate gene for tumor suppressor and transient neonatal diabetes mellitus (TNDM). In this study, a 2,238 bp fragment covering the complete coding region was obtained and deposited to GenBank (accession number: DQ288899). The reverse transcriptase-polymerase chain reaction (RT-PCR) indicated that PLAGL1 was expressed almost equally in heart, liver, spleen, lung, kidney, stomach, small intestine, skeletal muscle, fat, uterus and ovary. Comparing the sequences of Large White and Meishan pigs, a C-T transition in exon 6 was found. The polymorphism could be detected by TaqI and was genotyped in five purebreds (Large White, Landrace, Meishan, Tongcheng and Bamei). Association analysis was performed between the polymorphism and carcass traits in 276 pigs of a "Large White${\times}$Meishan" F2 resource population. As a consequence, significant associations of the genotypes with shoulder backfat thickness (SFT) and internal fat rate (IFR) were observed. Pigs with TT genotype had low SFT and high IFR compared with TC or CC genotypes.

• Journal of the Korean Society of Clothing and Textiles
• /
• v.24 no.3
• /
• pp.301-312
• /
• 2000

This study conducted an analysis of the middle aged women whose ages are from 35 to 59 years old in order to give those ladies much external satisfaction and covered body lines. I have come to present a few characters of body types as silhouettes which resulted in the factors of the upper bodies of middle aged women. I have adopted SAS(statistical analysis system) as a data process method of this survey. The followings are the results analysis. 1. The item of height have decreased, but increased in the items of thickness among the late middle aged women. 2. The body componant factor of middle aged women are extracted as 10 items, and the rate of their cumulative contribution is 78.9%. The first is the thickness of upper body, the second is the thickness of side upper body, the third is the height of upper body, the fourth is the length of upper body the fifth is width of upper body, the sixth is the projected rate of back, the seventh is the projected rate of breast, the eighth is the squared rate of shoulder, the ninth is the crooked rate of neck, and the tenth is the upper from of breast. 3. The body types of middle aged women can be classified into 4 types, and the followings are their characters. Type 1 is a standard type as the most common body character of the middle aged women, showing the appearing rate of 42.4%. Type 2 is a semi-curved body type like the curved figure of side body, showing the appearing rate of 34.5%. Type 3 is a reverse body type with the projected breast feature of front body, showing the appearing rate of 20.9%. Type 4 is a crooked body type with a tall height and crooked neck, showing the appearing rate of 2.2%.

• Fashion & Textile Research Journal
• /
• v.11 no.5
• /
• pp.788-798
• /
• 2009

This study aims to develop a highly reproducible, optimal frame design algorithm using variations in the curvature of armscye circumference, which will provide the basics for remodeling the 3D human body shape with the concept of reverse design used to develop total contents for the apparel industry. 1. The results of the experiment proved that ratio value was significantly efficient than absolute value of curvature variation to extract feature points in the armscye circumference 2. For the shoulder(1st and 2nd quadrant) and front armhole(3rd quadrant) parts of the armscye circumference, frame remodeling with the positive point of inflection led to the completion of a highly reproducible frame. 3. Similarly, even for the rear armhole part(4th quadrant) in the armscye circumference, it was found that frame remodeling using the positive maximum point of inflection resulted in highly reproducible body shape with the maximum point of inflection situated within the range of split angles $305^{\circ}{\sim}330^{\circ}$, while frame remodeling using simultaneously the two largest points of inflection including maximum point of inflection led to highly reproducible body shape with the maximum point of inflection out of the range $305^{\circ}{\sim}330^{\circ}$. 4. Based upon the optimal frame design algorithm developed in this study, section-specific feature points in the armscye circumference were extracted depending on the rate of curvature variation and remodeling with spline curves was conducted. The results indicate a remarkably high reproducibility(98.6%) and suggest that the algorithm developed in this study is suitable for human body modeling.

• Journal of Sasang Constitutional Medicine
• /
• v.27 no.1
• /
• pp.138-148
• /
• 2015

Objectives This study was conducted in order to analyze the distribution of Sasang constitution, herbmed, exterior-interior disease and concomitant symptoms of 36 facial nerve paralysis patients. Methods A clinical study was done on 36 patients, treated as facial nerve paralysis. Sasang constitution specialist diagnosed their constitution and gave prescription. Degree of improvement was evaluated by assessment scale or change of patient's complaint. Results 1) Distribution of sasang constituion : Taeeumin 21 patients, Soyangin 8 patients, Soeumin 7 patients. 2) Distribution of exterior-interior disease : Taeeumin and Soeumin were diagnosed as interior disease more than exterior disease, Soyangin was reverse. 3) Hyeongbangdojeok-san and Hyeongbangjihwang-tang (Soyangin), Galgeunhaegi-tang(Taeeumin) and Gwakhyangjeonggi-san(Soeumin) were used the most in each sasang constitution. 4) Seventeen patients had concomitant symptoms : digestive disorder, sleep disorder, tinnitus, hypertension, diplopia, itching, gout, neck-shoulder pain, constipation and dizziness. Conclusions Patients had different Sasang constitution and in the same Sasang constitution, they had different symptomatology.

• Korean journal of food and cookery science
• /
• v.30 no.6
• /
• pp.716-725
• /
• 2014

Gelatinization properties and the molecular structure of Korean waxy rice starchesisolated from two japonica types, Sinseonchal, and Dongjinchal, as well as an indica type, Hangangchal 1 were investigated. Sinseonchal is preferred cultivar for making Korean traditional rice cakes and cookies. Sinseonchal starch was the highest in crude protein, amylopectin, damaged starch contents, and water binding capacity among the cultivars tested. The initial pasting temperature ($72.75^{\circ}C$), peak (360.54 RVU), breakdown (162.21 RVU) and setback (30.83 RVU) viscosities of Sinseonchal had the highest values (p<0.05). Onset and peak temperatures by differential scanning calorimeter were also the highest in Sinseonchal. The molecular weight of Sinseonchal amylopectin was 5.46 107higher than those of the other cultivars, but its peak height and area were the lowest among them. The amylopectin peak by HPSEC showed a shoulder in the lower molecular weight portion and its relative area decreased in the following order; Sinseonchal > Dongjinchal > Hangangchal 1. On the branch chain length distribution of amylopectin, the proportion of DP13-24 and DP25-36 showed reverse trends, with higher japonica type amylopectin in DP13-24.

• Journal of Korean Society of Transportation
• /
• v.32 no.6
• /
• pp.662-674
• /
• 2014

In this study, accident frequency prediction models were constructed by collecting variables such as geometric structures, safety facilities, traffic volume and weather conditions, land use, highway design-satisfaction criteria along 780km (4,372 sections) of 4 lane-highways over 8 areas. As for models, a fixed parameter model and a random parameter model were employed. In the random parameter model, some influences were reversed as the range was expressed based on specific probability in the case of no fixed coefficients. In the fixed parameter model, the influences of independent variables on accident frequency were interpreted by using one coefficient, but in the random parameter model, more various interpretations were took place. In particular, curve radius, securement of shoulder lane, vertical grade design criteria satisfaction showed both positive and negative influence, according to specific probability. This means that there could be a reverse effect depending on the behavioral characteristics of drivers and the characteristics of highway sections. Rather, they influence the increase of accident frequency through the all sections.

• Korean Journal of Applied Biomechanics
• /
• v.15 no.3
• /
• pp.21-30
• /
• 2005

The purpose of this study is helps to make full use for perfect performance by grasping the defects of Tichonkich motion performed by athlete CSM For this, the study analyzed kinematical variables through Tichonkich motions performed at the first selection competition(1st trial) and final selection competition(2nd trial) for the dispatch to the 28th Athens Olympic Games using the three-dimensional cinematographical method with a high-speed video camera, and obtained the following results. 1. During Tichonkich motion, the execution time of up swing and the right hand moving to the left bar was shorter in the 2nd trial than the 1st one, while the execution time of down swing, the support of the left bar and the right hand moving to the right bar was longer in the 2nd trial than the 1st trial. 2. The horizontal position of COG in the 2nd trial was -35cm in the 1st stage, 42cm in the 3rd stage and 29cm in the 4th stage, that is, it showed a great swing focused on the circular movement compared to the 1st trial, while the vertical position of COG was -59cm in the 2nd stage, that is, it showed a small swing focused on a up and down movement. Also the 5th stage vertical position was 98cm, and the 6th stage vertical position was 95cm in the 2nd trial which were higher than those of the 1st trial, so it has provided magnificence required in the modern gymnastics. 3. And it was indicated that the horizontal velocity at the down swing phase proceeded forward more rapidly in the 2nd trial than that in the 1st trial, and the reverse ascent made a rapid vertical rise lessening left and right velocity change. And in the 5th stage, the 2nd trial was kept very slower in horizontal, vertical and left and right velocity that in the 1st trial, so it reached a handstand with leisurely movement. 4. In the 2nd trial, shoulder joint of the 1st, 2nd, 3rd stages kept a larger angle than that in the 1st trial, that is, it made a great swing while in the 1st trial, it showed a swing movement dependent on kick movement by the flexion and extension of hip joint. Also in the 2nd trial, the body formed a vertical posture with both hands supporting the left bar and hip joint was kept larger as $198^{\circ}$ and $190^{\circ}$ in the 5th and 6th stage than that in the 1st trial, so it made a handstand with the body uprightly stretched out, and magnificent and stable movement.