• Journal of Korean Society of Forest Science
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• v.111 no.2
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• pp.287-301
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• 2022

This study aims to propose a crane-tip control method to intuitively control the end-effector vertically or horizontally for improving the crane work efficiency and to confirm the control performance. To verify the control performance based on experimental variables, a laboratory-scale crane was manufactured using an electric cylinder. Through a forward and reverse kinematics analysis, the crane was configured to output the position coordinates of the current crane-tip and the joint angle at each target point. Furthermore, a method of generating waypoints was used, and a dead band using lateral boundary offset (LBO) was set. Appropriate parameters were selected using bang-bang control, which confirmed that the number of waypoints and LBO radius were associated with positioning error, and the cylinder speed was related to the lead time. With increased number of waypoints and decreased LBO radius, the positioning error and the lead time also decreased as the cylinder speed decreased. Using the proportional control, when the cylinder velocity was changed at every control cycle, the lead time was greatly reduced; however, the actual control pattern was controlled by repeating over and undershoot in a large range. Therefore, proportional control was performed by additionally applying velocity gain that can relatively change the speed of each cylinder. Since the control performed with in a range of 10 mm, it was verified th at th e crane-tip control can be ach ieved with only th e proportional control to which the velocity gain was applied in a control cycle of 20 ms.

• Journal of the Korean Geotechnical Society
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• v.26 no.11
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• pp.63-73
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• 2010

Monopiles, used as one foundation option for offshore wind turbines, are usually subjected to great cyclic lateral loads due to wind and wave. In this study, model pile load tests were performed using calibration chamber and three model piles with different pile lengths in order to investigate the behavior of laterally cyclic loaded piles driven into sand. Model test results show that the first loading cycle generates a bigger displacement than the following ones, and the permanent displacement of piles by one loading cycle decreases with increasing the number of cycles. 1-way cyclic loading causes the permanent displacement in the same direction as cyclic loading, whereas 2-way cyclic loading causes the permanent displacement in the reverse direction of initial loading. It is also observed that the permanent displacement of piles due to cyclic lateral loads increases with decreasing relative density of soil and with increasing the magnitude of cyclic loads. However, it is insensitive to the earth pressure ratio of soil and embedded pile length. In addition, based on the model pile load test results, equations for estimation of the permanent lateral displacement and rotation angle of piles due to 1-way cyclic lateral loads are proposed.

• 한국신재생에너지학회:학술대회논문집
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• 2010.06a
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• pp.246.1-246.1
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• 2010

본 연구는 바이오가스의 에너지효율성을 높이기 위한 연구로서 바이오가스 정제공정과 초저온액화공정을 통하여 액화바이오메탄을 생산하는 바이오가스 고질화기술개발 연구이다. 바이오가스 정제공정은 탈황, 제습, 흡착, 압축, $CO_2/CH_4$ 분리공정으로 구성하고, 초저온액화공정은 열교환기, $CO_2$ 제거설비, 질소냉매 공급공정으로 구성하여 혐기성소화조에서 발생하는 바이오가스($CH_4$ 농도: 60~65%, $H_2S$: 1,500~2,500ppm)를 $200Nm^3/hr$의 유량으로 인입시켜 액화바이오메탄을 생산하였다. 연구결과, 탈황공정에서는 가성소다 세정법을 이용하여 1,500~2,500ppm으로 인입되는 $H_2S$를 100ppm 이하로 제거한 후, 흡착법을 이용하여 $H_2S$를 완전히 제거하였다. 바이오가스에 포화된 수분은 냉각제습과 흡착제습공정을 통해 Dew point $-70{\sim}-90^{\circ}C$까지 제거하여 안정적으로 $CO_2/CH_4$ 분리공정에 인입시켰다. $CO_2/CH_4$ 분리공정은 흡착방식을 적용하여 $CH_4$ 순도가 95% 이상인 바이오메탄을 생산하였으며, 이때 메탄 회수율은 약 87%이였다. $CO_2$가 분리된 바이오메탄은 초저온액화공정을 이용하여 액화바이오메탄으로 전환시켰다. 이때 초저온액화공정은 Reverse Brayton cycle로 구성하였으며, 냉매로는 질소를 사용하였다. 액화바이오메탄의 생산은 바이오메탄을 등엔트로피과정인 단열팽창을 통하여 $-155{\sim}-159^{\circ}C$의 초저온으로 냉각되는 질소냉매와 열교환기에서 열교환시켜 이루어졌으며 그 생산량은 $3.46m^3$/day(1bar, $-161^{\circ}C$)이었다.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.9
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• pp.1257-1261
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• 2006

Pleiomorphic adenoma gene-like1 (PLAGL1) encodes a zinc-finger (ZF) protein with seven ZFs of the C2H2-type which is a regulator of apoptosis and cell cycle arrest, and also regulates the secretion of insulin. In both human and mouse, PLAGL1 is a candidate gene for tumor suppressor and transient neonatal diabetes mellitus (TNDM). In this study, a 2,238 bp fragment covering the complete coding region was obtained and deposited to GenBank (accession number: DQ288899). The reverse transcriptase-polymerase chain reaction (RT-PCR) indicated that PLAGL1 was expressed almost equally in heart, liver, spleen, lung, kidney, stomach, small intestine, skeletal muscle, fat, uterus and ovary. Comparing the sequences of Large White and Meishan pigs, a C-T transition in exon 6 was found. The polymorphism could be detected by TaqI and was genotyped in five purebreds (Large White, Landrace, Meishan, Tongcheng and Bamei). Association analysis was performed between the polymorphism and carcass traits in 276 pigs of a "Large White${\times}$Meishan" F2 resource population. As a consequence, significant associations of the genotypes with shoulder backfat thickness (SFT) and internal fat rate (IFR) were observed. Pigs with TT genotype had low SFT and high IFR compared with TC or CC genotypes.

• Journal of IKEEE
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• v.16 no.3
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• pp.274-282
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• 2012

In this paper, we propose the design of lighting control algorithm for intelligent LED lighting system. The proposed lighting control algorithm transmitted to MCU through a data bus the environmental information detected from respectively sensor node. The MCU control software was designed to determine the level maintained to depending on the set control method by comparing the results that calculated the dimming level using a signal value. Also, it was designed to be lighting by cross-performed periodically the rotation and reverse method by created fully symmetrical pattern using the control algorithm to LED lighting device. In this paper, the proposed lighting control algorithm improved the reliability of the data sent by designed the system that can be controlled lighting to stable, and it was maintained the event delivery ratio of 91%. Also, the lighting device was decreased the luminous intensity of 32%, the power consumption of 49%, and heat generation of 32%. As a result, it were could be improved the energy efficiency that the life-cycle of LED has been increased 50%.

• Tuberculosis and Respiratory Diseases
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• v.67 no.4
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• pp.303-310
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• 2009

Background: Elevated expression of cyclooxygenase-2 (COX-2) and Polo-like kinase-1 (PLK-1) is observed in a wide variety of cancers. Augmented expression of COX-2 and enhanced production of prostaglandin $E_2(PGE_2)$ are associated with increased tumor cell survival and malignancy; COX-2 has been implicated in the control of human non-small cell lung carcinoma (NSCLC) cell growth. PLK-1 siRNA induced the cell death of lung cancer cells and the systemic administration of PLK-1 siRNA/atelocollagen complex inhibited the growth of lung cancer in a liver metastatic murine model. COX-2 and PLK-1 are involved in proliferation and in cell cycle regulation, and there is a significant correlation between their interaction in prostate carcinoma. Methods: In this study, we investigated the pattern of COX-2 and PLK-1 expression in NSCLC, after treatment with IL-1$\beta$, COX-2 inhibitor and PLK-1 siRNA. Results: Expression of PLK-1 was decreased in A549 COX-2 sense cells, and was increased in A549 COX-2 anti-sense cells. Knock out of PLK-1 expression by PLK-1 siRNA augmented COX-2 expression in A549 and NCl-H157 cells. When A549 and NCI-H157 cells were treated with COX-2 inhibitor on a dose-dependent basis, PLK-1 and COX-2 were reduced. However, when the expression of COX-2 was induced by IL-1$\beta$, the production of PLK-1 decreased. Conclusion: These results demonstrate that COX-2 and PLK-1 are regulated and inhibited by each other in NSCLC, and suggest that these proteins have a reverse relationship in NSCLC.

• Journal of the Korean Institute of Telematics and Electronics
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• v.8 no.3
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• pp.1-14
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• 1971

A relay servo, one of the nonlinear sytsems, is inherently compact compared to a linear system for an equivalent control problem. The power element or actuator is not adjusted proportionally in accordance with an error signals but rather is switched abruptly between several discrete conditions. Usually switched conditions are off, full, forward or full reverse. The relay system is a particularly simple and compact one, but probably more effort has been expended on its analysis and design than on all other systems together. Early studies in the art were made by Goldfarb, austin, Oppelt and Kochenburger on the describing function method, which can be used as an approximate check on the stability of the system. The describing function method is based on the assumption that any periodic wave could be approximated as a fundamental one in wide ranges of practical applications. A relay servo system usually operates on a limit cycle condition as the loop gain increases. The stability analysis compensation or any improvement effort based on the describing function method sometimes may present considerable discrepancies on physically realized practical systems. An approach to exact periodic solutions of a relay servo system is much important for the analysis, design and system improvement. This paper dells with periodic solutions of a relay servo system on the basis of describing function and generalized chopper wave form which is composed of infinite number of harmonic series. Various ways of graphical representation were attempted to get periodic solutions, some of which have shown its validity in rapid approach to exact solutions and also in judgement of system behavior.

• The KSFM Journal of Fluid Machinery
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• v.19 no.6
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• pp.50-54
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• 2016

The performance of a scroll expander is the most important factor for the efficiency of small scale Organic Rankine cycle waste heat power generation systems. In this research, a scroll compressor was purchased and operated in reverse to function as a scroll expander. With air as a working fluid, a series of performance test were conducted on this expander by varying the inlet and outlet pressure. Secondly, We have tested through 2000 to 3500 rpm rotational speed to find the maximum power and efficiency of the expander. And last, It was observed in the initial experiments that the design of the expander's orbiting scroll wrap partially blocked the fluid intake which may have caused unnecessary flow resistance. To verify this theory, a small part of the scroll wrap was removed and the performance test was redone. It was observed that the lower back pressure assure the higher efficiency and power of expander and the rotational speed that shows maximum adiabetic efficiency of scroll expander is 69% at 2500 rpm. And by modified wrap of the scroll, we could get volume flow rate for 13% to 19% and power for 5% to 18% increased. But the maximum efficiency of the modified scroll was decreased 8%.

• Journal of Food Hygiene and Safety
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• v.33 no.1
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• pp.71-76
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• 2018

Norovirus is a leading cause of sporadic pathogenic non-bacterial gastroenteritis worldwide. For the detection of norovirus, reverse transcription real-time PCR (RT qPCR) has quickly become a major tool due to its sensitivity and specificity. However, accurate viral RNA extraction methods are essential for RT qPCR analysis. TRIzol reagents are used to extract RNA from biological materials and are therefore widely used for norovirus RNA extraction. In this study, the yield of viral RNA extraction using TRIzol from genogroup II (GII) among the human norovirus genogroup I (GI) and GII, and murine norovirus (GV) depended on the pH of the virus sample solution. The yield of RNA extraction was higher at the alkaline pH than in the acidic region compared with the Ct (threshold cycle) value of the real-time PCR. From the results of this study, it was found that the pH condition is very important for the quantitative analysis of norovirus by extracting GII RNA using TRIzol.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.178-178
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• 1994

Hepatitis B Virus (HBV) is a DNA virus with a 3.2kb partially double-stranded genome. The life cycle of the virus involves a reverse transcription of the greater than genome length 3.5kb mRNA. This pegenomic RNA contains all the genetic information encoded by the virus and functions as an intermediate in viral replication. Tumor suppressor p53 has previously been shown to interact with the X-gene product of the HBV, which led us to hypothesize that p53 may act as a negative regulator of HBV replication and the role of the X-gene product is to overcome the p53-mediated restriction. As a first step to prove the above hypothesis, we tested whether p53 represses the propagation of HBV in in vitro replication system. By transient cotransfection of the plasmid containing a complete copy of the HBV genome and/or the plasmid encoding p53, we found that the replication of HBV is specifically blocked by wild-type p53. The levels of HBV DNA, HBs Ag and HBc/e Ag secreted in cell culture media were dramatically reduced upon coexpresion of wild-type p53 but not by the coexpression of the mutants of p53 (G154V and R273L). Furthermore, levels of RNAs originated from HBV genome were repressed more than 10 fold by the cotransfection of the p53 encoding plasmid. These results clearly states that p53 is a nesative regulator of the HBV replication. Next, to addresss the mechanism by which p53 represses the HBV replication, we performed the transient transfection experiments employing the pregenomic/core promoter-CAT(Chloramphenicol Acetyl Transferase) construct as a reporter. Cotransfection of wild-type p53 but not the mutant p53 expression plasmids repressed the CAT activity more than 8 fold. Integrating the above results, we propose that p53 represses the replication of HBV specifically by the down-regulation of the pregenomic/core promoter, which results in the reduced DNA synthesis of HBV. Currently, the mechanism by which HBV overcomes the observed p53-mediated restriction of replication is tinder investigation.