The Jeungsan(1871-1909)'s revelation is the important meaning. Jeungsan hands down the two revelation for solution of Chosun postscript problem and presentation of Utopia. One time Suwun(1824~1864) is handed down and afterwords Jeongsan(1895-1958) after Jeungsan's Hwachon at 1917. Two revelations accomplish the day of the Jeungsan in public but the character is the countenance. The Jeungsan's revelation to Suwun solves present generation problems, the Jeungsan revelation to Jeongsan is continuously to do the day of the Jeungsan even after Hwachon. The Jeungsan stepped on the failure of the Donghak and on the upper lifestyle with the Cham-Donghag it opened the different side. The Cham-Donghag is concerned about the future Chosun to be the first-class country and Chosun Haewon is related with Danju Haewon. Danju Haewon is to be a fifty years religous study of Jeongsan. The Jeungsan's Gangse is to accomplish the Sungsajaein and it actualizes the appearance of Dotonggunja. Jeongsan is trying to do the religious activity about Sungsajaein and Dotonggunja as the Daedumok. The Jeungsan starts from revelation to the Suwun, to accomplish thought of the Cham-Donghag after Hwachon. The Jeungsan continuously has handed revelation led from religion activity, let Jeongsan do fifty years religious study. In the long run, it completes the fact that it accomplishes the outer space and the Chosun society of new setup, and a sacred religious work has been arranged the advocate of the Cham-Donghag through the Jeongsan by Woodang(1917-1996)'s Daesoonjinrihoe(1969).
Objectives/Methods : To analyze the effect of Injinchunggantang(IJCGT) to Interferon-${\alpha}/{\beta}$ signal transmission system in HepG2 cells, HepG2 Cell were treated with IJCGT. Also, revelation of MxA, 2'5'-OAS mRNA leaded by Interferon-${\alpha}/{\beta}$ and revelation and activation of Jak1, TYK1, and STAT 1, all main signal transmission factors, were analyzed. Results : The analysis resulted in the following 1. With interferon ${\alpha}/{\beta}$ there was no affect cell propagation of Hep G2 cells. With IJCGT alone, cell propagation of HepG2 was promoted, and cell propagation control function was recovered. 2. With interferon ${\alpha}/{\beta}$ cell death was unaffected. With IJCGT apoptosis of HepG2 cell was restrained, and the cell's reaction to interferon was unaffected. 3. With interferon ${\alpha}/{\beta}$ treatment mRNA revelation of MxA and 2'5'-OAS was induced. When HepG2 cells were injected with IJCGT without interferon ${\alpha}/{\beta}$ treatment, mRNA revelation of MxA and 2'5'-OAS increased in proportion to the treatment density. With pre-treatment of IJCGT, leaded with interferon ${\alpha}/{\beta}$, promoted revelation of MxA, 2'5' -OAS mRNA. 4. Though mRNA revelation of lakl, TYK1 and STAT1 was unaffected with IJCGT, activation of STAT1 was promoted with an increase of phosphorylation of STAT1 protein. With pre-treatment of IJCGT, Jak1, TYK2, STAT1 phosphorylation, leaded with interferon, strengthened. 5. TNF-a, IL-1b and LPS present, revelation of MxA and 2'5'-OAS mRNA leaded by interferon was restrained when HepG2 cells were treated with IJCGT, and the interferon signal transmission system restraint action leaded by inflammatory cytokines was moderated. Conclusion : These results support a role for IJGCT in promotion of anti-virus action through maintainance of the liver's sensibility toward interferon. A clinical study of an interferon treated patient treated also with IJGCT is needed to determine its efficacy.
Proceedings of the Korean Institute of Building Construction Conference
/
2006.11a
/
pp.91-94
/
2006
The way to shorten a construction period is considered to an very important technology development element as reducing the formwork removal periods with promoting strength revelation own concrete. This study executed experiment to review usability of early strength revelation chemical admixture which is judged in ways effective with premature removal of form about concrete. Use of early strength revelation AE water reducin admixture is apperaing so that strength revelation by early hydration promotions is excellent. The results of being applied proposed work process are that compressive strength are appeared more than 5MPa within 16 hours so that removal of vertical form was possible. the concrete compressive strength satisfied with a more than 2/3 of specified concrete strength for removal of horizontal form are appeared in 42 hours of 27 MPa proportioning strength, in 36 hours of 30, 35 MPa proportioning strength so that the 6 days cycle time of concrete structural frame work is cut by 2 days as shortening delayed period in works of removing slab forms. So construction cost reductions and a construction period shortening are judged so that it is possible.
The objective of this research is to suggest the creativity revelation model and to verify the relationships among knowledge capabilities and creativity processes including exploration and exploitation. Also, we investigate whether there are differences in creativity revelation processes from the perspective of psychological empowerment. To achieve the purpose of the research, a survey was conducted targeting employees of software development companies that require creativity in work performance. Empirical results show that knowledge capabilities have positive effect on creativity revelation processes. The notable point of the results is the role of psychological empowerment such that individuals with high psychological empowerment have more exploration-centric revelation, and those with low psychological empowerment have more exploitation-centric on the other hand. These results are interpreted that the behavioral patterns of organizational members may vary depending on the level of psychological empowerment in the creativity revelation, and therefore could suggest several managerial implications regarding creativity management and organizational development in an environment where convergence becomes more important.
Proceedings of the Korea Concrete Institute Conference
/
1997.10a
/
pp.157-165
/
1997
In this experiment, there is a purpose to analysis the relationship of feature of compressive strength after fixing of remarkable element under the condition of initial curing temperature. According to this experiment, we get to the fallow result. In case of highest curing temperature, 3-day-strength become high but last revelation of strength become low among the condition of initial curing temperature, the highest curing temperature have an effect on revelation of strength by the application of cumulative temperature, we can get the shape of revelation of strength.
Park, Sang-Hyun;Kim, Jin-Sung;Yoon, Sang-Hyub;Ryu, Bong-Ha
The Journal of Internal Korean Medicine
/
v.27
no.2
/
pp.379-393
/
2006
Objectives: We are aimed to identify anti-tumor effects of Curcuma aromatics on some kinds of cancer cells through molecular biologic methods. Materials & Methods: We used 4 kinds of cancer cell lines such as lung cancer cells(AS49), cervical cancer cells(HeLa), glioma cancer cells(A172) and prostate cancer cells(PC3). We treated the boiled extract of Curcuma aromatica $5{\mu}g,\;10{\mu}g$ to cultural media(ml) for 24 hours. We measured the cytotoxicitv on 4 kinds of cancer cells through tryphan blue exclusion test and the suppressive effect on viability of 4 kinds of cancer cells via MTT assay. We measured change of mitochondria membrane potential via flow cytometry. The quantitative RT-PCR was used to examine the effect on the revelation of Bcl-2 and Bax which are genes related to apoptosis. We examined the effect on the revelation of Bcl-2 Protein and Bar protein by western blot analysis. Results : In the experiment of tryphan blue exclusion test, the extract of Curcuma aromatica showed more significant killing effect on AS49, HeLa than the control group with density dependent manner, which was statistically significant. In the experiment of MTT assay the extract of Curcuma aromatica showed more suppressive effect on viability of A549, HeLa than the control group with density dependent manner, which was statistically significant. Curcuma aromatica induced apoptosis by decreasing the membrane potential of mitochondria in A549, HeLa. In the experiment of the revelation of genes related to apoptosis, the revelation of Bcl-2 decreased and the revelation of Bax increased in A549, HeLa treated with Curcuma aromatica with dose dependent manner. In the experiment of the revelation of protein related to apoptosis, the protein levels of Bcl-2 decreased and the protein levels of Bax increased in AS49, HeLa treated with Curcuma aromatica with dose dependent manner. Conclusions: From this study, we can infer that Curcuma aromatica has anti-tumor effect on lung cancer cells and uterine carcinoma cells but not on glioma cells and prostate cancer cells.
Objectives : The Purpose of this study was to identify anti-tumor effects of Curcuma longa L. on some kinds of cancer cells through molecular biologic methods. Materials & Methods : We used 4 kinds of cancer cell lines such as glioma cells(A172), cervical cancer cells(HeLa), Prostate cancer cells(PC3), lung cancer cells(A549). We injected the boiled extract of Curcuma longa L. $5{\mu}g,\;10{\mu}g$ to culture media(ml) for 24 hours. We measured the cytotoxic effect on 4 kinds of cancer cells through trypan blue exclusion test and the suppressive effect on viability of 4 kinds of cancer cells via MTT assay. We measured the change of mitochondria membrane potential via flow cytometry. The quantitative RT-PCR was used to examine the effect on the revelation of Bcl-2 and Bax which genes are related to apoptosis. We examined the effect on the revelation of Bcl-2 protein and Bax protein by western blot analysis. Results: 1. Extract of Curcuma longa L. showed significant cytotoxic effect on A172, HeLa, PC3 compared to the control group with density dependent manner. 2. Extract of Curcuma longs L. showed significant suppressive effect on viability of A172, HeLa, PC3 compared to the control group with density dependent manner. 3. Curcuma longs L. induced apoptosis by decreasing the membrane potential of mitochondria in A172, HeLa, PC3. 4. In the test about the revelation of genes related to apoptosis, the revelation of Bcl-2 decreased and the revelation of Bax increased in A172. HeLa, PC3 treated with Curcuma longa L. with density dependent manner. 5. In the test about the revelation of protein related to apoptosis, the protein levels of Bcl-2 decreased and the protein levels of Bax increased in A172, HeLa, PC3 treated with Curcuma longa L. Conclusions: This experiment shewed that Curcuma longs L. has anti-tumor effect on glioma, cervical, Prostate cancer cells except on lung cancer. We hope that anti-tumor effects of Curcuma longa L. will be more Practically identified.
My essay aims at reading Ginsberg's "Howl" and "Kaddish" with the concept of 'shaman-prophet-poet' to illustrate the dynamic relationship between his poetics and radical politics. Throughout his widely-ranging career, Ginsberg represents himself as a poet-prophet and commands a typical rhetoric of revelation as a way of decentering Cold War orthodoxies. While well aware of the oppressive and pervasive power of the dominant post-war ideologies, he adopts 'madness' to oppose conventional political, social, and religious institutions; by way of entering into the madness of this world and actively engaging himself as a victim, he can finally heal both himself and the world. This dual function of poet characterizes his rhetoric of revelation, but it doesn't appeal to the mainstream of American critical ideology where the post-structural approach to language and subject gives a skeptical look at any account of active human agency and humanistic belief in the possibility of language. In "Howl" and "Kaddish," Ginsburg persuades the reader of the truth of his own vision through the convincing and realistic portraits of his contemporaries as well as his own mother and family. Different from his visionary predecessors such as Emerson and Whitman, Ginsberg knew the difficulty of a negotiation between history and divine vision, and attempted to imbricate his family, friends, and even the larger social and political units within his visionary experience in order to avoid naive idealism, escapism, or solipsism. Furthermore, he deconstructs the Logos of Western prophecy and replaces it with the groundless identity and the nontheistic epistemology of Buddhism, which, in turn, leads to emptying his powerful language of absolutist meaning and prevents his prophecy from becoming re-reified as divine essentialism. Ginsberg's idea of poet and poem revitalizes the skeptical view on language and literary representation of our contemporary critical community which is unwilling to engage the experimental scope of his radical prophecy.
Kim, Min-Ae;Kim, Mi-Bo;Ko, Woo-Shin;Yoon, Hwa-Jung
The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
/
v.21
no.3
/
pp.69-81
/
2008
Objective : The purpose of this study was find out the anti-allergic effects of SESHINGO on the allergic rhinitis. Methods : Cell viability was evaluated by the MTT assay. Cells were treated with the indicated concentration of SSG. $TNF-\alpha$ concentration was measured from cell supernatants using ELISA method. IL-4 concentration was measured from cell supernatants using ELISA method.$IFN-\gamma$ concentration was measured from cell supernatants using ELISA method. Total RNA was isolated, $TNF-\alpha$ and IL-4 mRNA expression was detected by RT-PCR analysis Total RNA was isolated, COX-1, COX-2 mRNA was analyzed by RT-PCR analysis. Results : 1. $\beta$-hexosaminidase release in RBL-2H3 cells were decresed by SESHINGO attentively. 2. Secreting ration of $TNF-\alpha$was restrained in RBL-2H3 cells by SESHINGO attentively. 3. Secreting ration of IL-4 was restrained in RBL-2H3 cells by SESHINGO attentively 4. Revelation of $TNF-\alpha$ mRNA was decresed in RBL-2H3 cells as concetration. 5. Revelation of.IL-4 mRNA was decresed in RBL-2H3 cells as concetration. 6. Revelation of COX-2 mRNA was decresed in RBL-2H3 cells. Conclusion : According to above results, to evaluate the effect of SSG on the anti-allergic effects.
Kang Woo-Sung;Kim Young-Chul;Lee Jang-Hoon;Woo Hong-Jung
The Journal of Internal Korean Medicine
/
v.25
no.1
/
pp.28-45
/
2004
Objectives : The main purpose of this study is to evaluate the effect of Injinchunggan-tang on $TNF-{\alpha}$ signal transmission system. Materials and Methods : We analyzed the following with quantitative RT-PCR method; the effect of Injinchunggan-tang on secretion of $TNF-\alpha$ mRNA/protein and stability, the effect on gene revelation that consists of signal transmission system (TRAIL, NIK, A20, TRADD, RAIDD, RIP TNFR-I, TNFR-II, TRAF1, TRAF2, FADD), the one on activation of p38, Erk1/2 MAPK and the rate of nuclear $NF-{\kappa}B/cytosolic\;NF-{\kappa}B$ in HepG2 cell. We also analyzed the inhibitory effect of Injinchunggan-tang on the apoptosis of HepG2 cell that $TNF-{\alpha}$ induces and the $NF-{\kappa}B$ restraint effected by transfection of $I{\kappa}B{\Delta}N$ through tryphan blue exclusion assay. Results : Injinchunggan-tang prohibits revelation of $TNF-{\alpha}$ mRNA in HepG2 cell and the creation of protein. However, it has no effect on the stability of $TNF-{\alpha}$ mRNA. While it did not have any effect on the generation of TRAIL, NIK, A20, TRADD, RAIDD and RIP genes, Injinchunggan-tang reduces the revelation of TNFR-I, TNFR-II, TRAF1, TRAF2 and FADD genes. It has been confirmed that Injinchunggan-tang restraints the revelation of $TNF-{\alpha}$ mRNA that is promoted by ethanol, acetaldehyde, lipopolysaccharide, in proportion to the treatment density and time. It activated $NF-{\kappa}B$ of HepG2 cell and promoted activation of $NF-{\kappa}B$ that is occurred by $TNF-{\alpha}$. It has been observed that the restraint effect against the $TNF-{\alpha}$ inducing apoptosis is lost when it is intercepted the function of $NF-{\kappa}B$ in HepG2 cell. Conclusion: It has been confirmed that Injinchunggan-tang has restraining effect against the revelation of $TNF-{\alpha}$ and mRNA that is constituent element of TNF-a signal transmission system. It also has been revealed that it restraints the activation of p38, Erk1/2 by $TNF-{\alpha}$. Through this prohibiting effect, it is inferred that it restraints signal transmission among various cells that are related to inflammation reaction. Meanwhile, Injinchunggan-tang protects liver cell from apoptosis that is caused by $TNF-{\alpha}$, by maintaining the activating function for $NF-{\kappa}B$.
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