• Title/Summary/Keyword: Reticulum

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An Electron Microscopic Observation of Some Membrane Structures of Lens Fibers of Regenerating Lens in Triturus pyrrhogaster (Triturus pyrrhogaster 에서 再生되는 水晶體纖維 細胞中 膜構造에 관한 電子顯微鏡的 觀察)

  • Sung, Hwan-Soon;Sung, Hwan-Sang
    • The Korean Journal of Zoology
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    • v.11 no.1
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    • pp.5-12
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    • 1968
  • The membrane structures were electron microscopically studied in the elongating lens fibers of the regenerating lens of adult Triturus pyrrhogaster. Observations were focused on the endoplasmic reticulum and mitochondria. The endoplasmic reticulum developed in the vicinity of the nucleus with active blebbing of the outer membrane. At the same time, the concentration of mitochondria around the rough-surfaced endoplasmic reticulum near by the nucleus was always observed. Both endoplasmic reticulum and mitochondrion undergo disintegration in the apical portion apart from the nucleus. Some considerations were discussed with reference to published data.

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Subcellular Localization of Capsaicin-Hydrolyzing Enzyme in Rat Hepatocytes (Capsaicin 가수분해효소의 흰쥐 간세포내 소재확인)

  • Park, Young-Ho;Lee, Sang-Sup
    • YAKHAK HOEJI
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    • v.38 no.1
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    • pp.12-19
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    • 1994
  • Capsaicin(8-methyl-N-vanillyl-6-nonenamide) is the principal pungent component of Capsicum fruits. This work is directed to the capsaicin-hydrolyzing enzyme playing a key role in the rate limiting and critical step of capsaicin metabolism. In order to get precise information on the enzyme's subcellular location, rat liver homogenate was divided into six subcellular fractions by differential centrifugation technique: crude nuclear pellet, PNS(post nuclear supernatant) fraction, lysosomal pellet, cytosol, Tris wash fraction, micrisomes. Capsaicin-hydrolysing enzyme activity was analysed by high performance liquid chromatography(HPLC). This enzyme was found at the highest specific activity in the microsomal fraction and co-distributed with marker enzymes of the endoplasmic reticulum, NADPH-cytochrome c reductase and nucleoside diphosphatase. This is compatible with the result of ninhydrin color reaction of vanillylamine, primary metabolite of capsaicin hydrolysis, on thin layer chromatography(TLC). This enzyme is most active at pH $8.0{\sim}9.0$. Definite subcellular location of this enzyme will make it easy to proceed with further study.

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Endoplasmic Reticulum Stress Response of Bombyx mori Calreticulin

  • Goo, Tae-Won;Park, Soojung;Jin, Byung-Rae;Yun, Eun-Young;Kim, Iksoo;Nho, Si-Kab;Kang, Seok-Woo;Kwon, O-Yu
    • Proceedings of the Korean Society of Sericultural Science Conference
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    • 2003.10a
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    • pp.78-79
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    • 2003
  • To further understanding of the role of calreticulin in insects, we have isolated a cDNA of calreticulin from silkworm, Bombyx mori. The cDNA encodes a 398 amino acid residues of B. mori calreticulin with endoplasmic reticulum retentional HDEL motif at its C-terminus, and a predicted molecular mass of 45801 Da. The B. mori calreticulin shows high protein homology with those of G mellonella (88%), A. aegypti (71%) and H. sapiens (63%). (omitted)

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Insulin-like Growth Factor-1 (IGF-1) Gene Expression Is Enhanced under Hypothermia but Depressed under Additional Ischemic Stimulus

  • Kwon, O-Yu;Kwon, Kisang;Yu, Kweon;Kim, Seung-Whan
    • Biomedical Science Letters
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    • v.21 no.2
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    • pp.126-130
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    • 2015
  • There are several studies that show hypothermia improves cellular ischemia damages on experimental and clinical bases. However, its exact molecular mechanisms are unclear. In this study, we demonstrate that hypothermia induced insulin-like growth factor 1 (IGF1) gene expression, and its expression was dramatically decreased under ischemic insults. It was also demonstrated that hypothermia activated endoplasmic reticulum (ER) stress sensors especially both the phosphorylation of $eIF2{\alpha}$ (eukaryotic translation initiation factor 2 alpha) and ATF6 (activating transcription factor-6) proteolytic cleavage. However, the factors of apoptosis and autophagy were not associated with hypothermia. We suggest that hypothermia-treated IGF1 gene expression after ischemia may show a good possibility for the development of treatments and diagnostic methods in cerebral ischemic damages.

Studies on the Toxicity of $\delta$-endotoxin of Bacillus thuringiensis to the Several Tissues of Hyphantria cunea Drury (미국흰불나방(Hyphantria cunea Drury)에 대한 Bacillus thuringiensis 내독소단백질의 독성효과에 관한 연구)

  • 전향미;조자향;강석권;서숙재
    • Journal of Sericultural and Entomological Science
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    • v.37 no.1
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    • pp.62-67
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    • 1995
  • Ultrastructural changes of tissues caused by Bacillus thuringiensis var. kurstaki $\delta$-endotoxin intoxication of Hyphantria cunea were observed by transmission electron microscopy. Bt $\delta$-endotoxin crystals induced the disruption of microvilli, vacuolation of cytoplasm, changes in the cisternae of the endoplasmic reticulum, disappearance of basal striations, loss of ribosomes, and changes in the configurations of mitochondria in the columnar cell of midgut. The fat body cells also showed spherical endoplasmic reticulum and distorted mitochondria, and then the cells were destroyed.

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Ultrastructural Changes and Formation of Storage Materials in Endosperm Cells during the Seed Formation of Panax ginseng C.A. Meyer (인삼(Panax ginseng C.A. Meyer)의 종자형성에 있어서 배유세포의 미세구조의 변화 및 저장물질의 형성)

  • 유성철
    • Journal of Plant Biology
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    • v.34 no.3
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    • pp.201-213
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    • 1991
  • This study has been carried out to investigate the ultrastructural changes, formation of storage materials in endosperm cells with electron microscope during the seed formation of Panax ginseng C.A. Meyer. In the early stage of seed formation with green seed coat, the endosperm was cellular type. Cell plate was largely composed of dictyosome vesicles in early stage of wall formation after mitosis. Central vacuole was gradually subdivided into several small-sized vacuoles. During the differentiation of plastids, some proplastid was replaced by amyloplast with starch grains and lamellar structure. A number of mitochondria with well developed cristae were distributed in cytoplasm. Rough endoplasmc reticulum, dictyosome, microbody, free ribosomes and polysomes were evenly distributed in cytoplasm. Spherical spherosomes were formed from dictyosome containing the lipid materials of even electron density. Protein bodies were formed by interfusing between vacuoles and vesicles derived from rough endoplasmic reticulum which contained the amorphous protein of high electron density.

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