• Journal of Food Hygiene and Safety
• /
• v.39 no.3
• /
• pp.209-220
• /
• 2024

Cavier is graded as Beluga, Osetra, and Sevruga based on the species of sturgeon (Acipenser sinensis). In this study, we developed an analytical method for determining the grade of black caviar using DNA barcodes and KASP markers. To identify the sturgeon species, ten black caviar samples were collected, and a reference DNA barcode library was developed using five genes (namely, 16S ribosomal RNA, cytochrome b, cytochrome c oxidase subunit I, cytochrome c oxidase subunit II, and NADH dehydrogenase subunit 5 genes). To develop the KASP markers, we selected 11 markers that could distinguish between the five grades of black caviar. As a result, specific markers for each of the targeted caviars were clustered into FAM-positive sections. DNA barcoding and the KASP assay revealed that one Beluga, six Osetra, and three Sevruga were identified among the ten caviar samples. Moreover, we found that the sturgeon species were mislabeled in two products. Here, we aimed to develop a KASP assay based on SNP that allows rapid and easy identification of caviar grade. These methods are expected to contribute to preventing the distribution of illegal products.

• KIPS Transactions on Software and Data Engineering
• /
• v.3 no.12
• /
• pp.553-564
• /
• 2014

In this paper, we propose the system for automatic generation of issue analysis report based on social big data mining, with the purpose of resolving three problems of the previous technologies in a social media analysis and analytic report generation. Three problems are the isolation of analysis, the subjectivity of experts and the closure of information attributable to a high price. The system is comprised of the natural language query analysis, the issue analysis, the social big data analysis, the social big data correlation analysis and the automatic report generation. For the evaluation of report usefulness, we used a Likert scale and made two experts of big data analysis evaluate. The result shows that the quality of report is comparatively useful and reliable. Because of a low price of the report generation, the correlation analysis of social big data and the objectivity of social big data analysis, the proposed system will lead us to the popularization of social big data analysis.

• Proceedings of the Korean Institute of Surface Engineering Conference
• /
• 2017.05a
• /
• pp.156-156
• /
• 2017

In this work, analysis of biocompatible TiO2 oxide multilayer by the XPS depth profiling was researched. the manufacture of the TiO2 barrier-type multilayer was accurately performed in a mixed electrolyte containing HAp, Pd, and Ag nanoparticles. The temperature of the solution was kept at approximatively $32^{\circ}C$ and was regularly rotated by a magnetic stirring rod in order to increase the ionic diffusion rate. The manufactured specimens were carefully analyzed by XPS depth profile to investigate the result of chemical bonding behaviors. From the analysis of chemical states of the TiO2 oxide multilayer using XPS, the peaks are showed with the typical signal of Ti oxide at 459.1 eV and 464.8 eV, due to Ti 2p(3/2) and Ti 2p(1/2), respectively. The Pd-3d peak was split into Pd-3d(5/2) and Pd-3d(3/2)peaks, and shows two bands at 334.7 and 339.9 eV for Pd-3d3 and Pd-3d5, respectively. Also, the peaks of Ag-3d have been investigated. The chemical states consisted of the O-1s, P-2p, and Ti-2p were identified in the forms of PO42- and PO43-. Based on the results of the chemical states, the chemical elements into the TiO2 oxide multilayer were also inferred to be penetrated from the electrolyte during anodic process.The structure characterization of the modified surface were performed by using FE-SEM, and from the result of biological evaluation in simulated body fluid(SBF), the biocompatibility of TiO2 oxide multilayer was effective for bioactive property.

• Tribology and Lubricants
• /
• v.32 no.4
• /
• pp.113-118
• /
• 2016

As an economic, high quality, and highly reliable gear with low noise and low vibration is demanded, an overall finite element analysis regarding a gear is required. Also, an infrared thermography test, which is a quantitative testing technique, is demanded for safety and longer lifespan of gear products. In order to manufacture a gear product or to determine safety of a gear being used, it is necessary to precisely determine ingredients of a material constituting a gear and detect any internal defect. This study aims to realize a design that minimizes the spur gear displacement with respect to power during its rotation and ensures the spur gear control capacity by using a 3D model and the midasNFX program. This facilitates the assessment of the possibility of cracking by evaluating the stress intensity and focusing on the integrity of the spur gear. We prepare the specimen of the spur gear based on the possibility of cranking as per the result of the structural interpretation from an infrared ray thermal measuring technique. After cooling the spur gear, we perform experiments using thermography and halogen lamps and analyze the temperature data according to the results of the experiment. In the experiment which we use thermography after cooling, we find a rise in the temperature of the room. As a result, the defective part show temperatures lower than their surroundings while the normal parts have temperatures higher than the defective parts. Therefore, it possible to precisely identify defective part owing to its low temperature.

• Journal of the Korean Society of Environmental Restoration Technology
• /
• v.14 no.2
• /
• pp.107-115
• /
• 2011

The purpose of the study is to analyze the environmental characteristics of fish acute toxicity that is dependent on the harmfulness of ACQ (Alkaline Copper Quat)-Treated Wood and Oryzias latipes mortality in a comprehensive way, provide objective verification method on the eco-toxicity and environment-friendliness of landscaping materials and methods, and utilize it as a basic datum for evaluation criteria. The main results are summarized as follows : 1. As a result of analysis on the harmfulness characteristics, each experimental plot showed different values respectively. In particular, it has been found that in proportion to the volume of testing materials, COD and Cu increases at a constant rate, compared to the input water. In the plot C with three testing materials, COD increased 67 times more than that of the input water, and Cu increased up to 12.36mg/L. 2. In case of fish toxicity, plot C, B, A all showed a mortality rate of 100%, indicating that fish toxicity is strong. In particular, the mortality rate of each plot within the initial time of one and a half hour showed clearly, which suggests that the fish toxicity is influenced by the increased concentration of hazardous substances depending on the volume ratio of testing materials. 3. As a result of comparison and analysis on the harmfulness and fish toxicity, the harmfulness showed different values on each experimental plot but, we found that the changing rate of values of toxicity of COD and Cu is mutually similar to that of mortality in the initial hour according to the experiment of fish toxicity, which shows that COD and Cu are major factors to increase fish toxicity.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.5 no.2
• /
• pp.136-140
• /
• 2000

A sensitive and quantiative method for the structural analysis of oligosaccharide was established for the glycoform analysis of glconproproteins. Inthis study, n-linked oligosaccharides of human IgG and bovine transferin were analyzed for the evaluation of the methydrate moiety ofthe method. Chrbohydrate moiety of glycoprotein was relased by hydrazinolysis and purified by paper chromatography. The oligosaccharides were labeled with a fluorescent bye, 2-aminobenzamide, for the enhancement of detection sensitivity. sialylated (acidic) oligosaccharides were separated from neutral oligosaccharide by employing a strong anion-exchange column(MonoQ) followed by the treatment with sialidase. Enzymatically desiayated fractions and neutral fractions of oligosaccharides were applied to normal-phase HPLC to resolve the peaks according to glucose unit (GU). The structure of separated molecules was further determined by sequential digestion with exoglycosidases. As a result, disialylated biantennary complextype oligosaccharide was found to be a major sugar chain in bovine transferrin (63%). In human IgG, core fucosylated asialobiantennary complex oligosaccharides were dominant. These results coincided well with reported results.

• Proceedings of the Computational Structural Engineering Institute Conference
• /
• 2006.04a
• /
• pp.1001-1008
• /
• 2006

The main objective of this paper is to compare economical effectiveness of typical methods for checking stability in principal components of steel cable-stayed bridges. Elastic and inelastic buckling analyses are carried out for frame-like numerical models of cable-stayed bridges. The axial-flexural interaction equations prescribed in AASHTO Allowable Stress Design (ASD) and AASHTO Load and Resistance Factor Design (LRFD) are used in order to check the stability of principal components. Parametric studies are performed for numerical models which have the center span length of 300m, 600m, 900m and l200m with different girder depths. Peak values of the interaction equations are calculated at the intersection point between girders and towers. These peak values are considered as a major factor to design of principal components of cable-stayed bridges. As a result, more economical design for girders and towers can be feasible using the inelastic buckling analysis. In addition, LRFD codes are more economical about 20% on the average than ASD codes for all numerical models of cable-stayed bridges.

• International Commerce and Information Review
• /
• v.3 no.2
• /
• pp.71-92
• /
• 2001

The number of internet electronic commerce users has increased steadily and EC is now considered not only as the communication tools, but also as the economic activity. But in spite of these global trends, current studies limited to managerial approach are insufficient in the evaluation of EC policies and situations. The purpose of this study are to present satisfaction of customers who will operate foreign internet shopping mall, and to analysis after-purchase behavior on EC satisfaction. As a result of making regression analysis, for its satisfaction. the hypotheses on "Shopping Mall Contents", "Information supply", "Convenience of order process", "Economy", "The reliability", "Customer Service" were accepted at the p<.0.05 level of significance, for its after purchase behavior, the hypotheses on "Shopping Mall structure", "Convenience of order process", "Economy", "The reliability", etc factors were accepted at the p<0.05 level of significant in 4 factors. In order words, electronic commerce satisfaction became greater when better convenience and economy were provided, the ethics were observed better, more diverse information was supplied and better system support was given, and those who had a higher educational background or better perception of electronic commerce felt more satisfaction. Finally, more usefulness of electronic commerce led to bigger satisfaction after purchase.

• Phonetics and Speech Sciences
• /
• v.7 no.2
• /
• pp.47-54
• /
• 2015

The objectives of this study are to research the frequency of depression symptom in patients with voice disorders and to investigate parameters associated with depression from voice evaluation. A hundred ninety six patients(106 males and 90 females) who had been diagnosed with voice disorders first in their lifetime were selected. All the patients were examined by laryngeal stroboscopy. For depression and voice study, personal interview, acoustic and aerodynamic analysis, voice handicap index(VHI), reflux symptom index(RSI), and beck depression index(BDI) were done respectively. Mild to severe BDI were seen in 26.2%(52 patients) of the whole patients. A BDI mean score of female patients was $8.8{\pm}7.5$ which was higher than that of male patients($5.6{\pm}6.6$), the difference observed being statistically significant(p<0.001). In the acoustic analysis, the score of sent_duration parameter was increasing in the patients with depression, which was significantly higher than the score of the patients without depression(p<0.05). In the addition, the scores of VHI and RSI were higher in the patients with depression(p<0.001). Our findings suggest that the prevalence of depression in patients with voice disorders is related to female, speaking velocity, and self-questionnaire. This result can be used for psychologically based approach to therapy.

• Journal of Ginseng Research
• /
• v.38 no.1
• /
• pp.59-65
• /
• 2014

Discriminating between two herbal medicines (Panax ginseng and Panax quinquefolius), with similar chemical and physical properties but different therapeutic effects, is a very serious and difficult problem. Differentiation between two processed ginseng genera is even more difficult because the characteristics of their appearance are very similar. An ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF MS)-based metabolomic technique was applied for the metabolite profiling of 40 processed P. ginseng and processed P. quinquefolius. Currently known biomarkers such as ginsenoside Rf and F11 have been used for the analysis using the UPLC-photodiode array detector. However, this method was not able to fully discriminate between the two processed ginseng genera. Thus, an optimized UPLC-QTOF-based metabolic profiling method was adapted for the analysis and evaluation of two processed ginseng genera. As a result, all known biomarkers were identified by the proposed metabolomics, and additional potential biomarkers were extracted from the huge amounts of global analysis data. Therefore, it is expected that such metabolomics techniques would be widely applied to the ginseng research field.