• The Korean Journal of Mycology
• /
• v.29 no.1
• /
• pp.9-14
• /
• 2001

Colletotrichum species are important fungal pathogens that cause great damages on various host plant species worldwide. In Korea, Colletotrichum species cause massive economic losses on apple, peach, grape, and especially, sweet persimon productions. In the past, identification of the pathogen and the studies on the genetic relationships among the pathogenic isolates were mainly based on morphology, cultural characteristics, and the difference in pathogenicity. However, in recent years, these traditional methods have been replaced with molecular methods including AFLP. AFLP method with the merits of both RAPD and RFLP has been widely used for the genetic relationship studies of various organisms. Therefore, in this study, AFLP method was employed for the studies of genetic relationships among the different isolates of Colletotrichum species collected from various parts of sothern Korea. As a result, two specific band pattern groups were observed among different isolates of Colletotrichum species.

• Archives of Plastic Surgery
• /
• v.35 no.5
• /
• pp.553-559
• /
• 2008

Purpose: The lateral orbital wall fractures have been previously classified by some authors. As there are some limitations in applying in their own classifications, we hope to present a refined classification system of the lateral orbital wall fracture and to identify the correlation between the specific type of the fracture and clinical diagnosis. Methods: The facial bone CT scans and medical records of 78 patients with the lateral orbital wall fractures were reviewed in a retrospective manner. The classification is based on the CT scan. In type I, the fracture and its segments are away from the lateral rectus muscle and in type II, they are next to or slightly pushing the muscle in axial CT scan. In type III, the fracture segments compress and displace the longitudinal axis of the muscle or the optic nerve in axial view of CT scan. Type IV fracture includes multiple fractures found around the orbital apex or optic canal in coronal view of CT scans of the type I and type II fractures. Results: The most common fracture pattern was type I(43.6%), followed by type IV(29.5%), type II(20.5%), and type III(6.4%). As diplopia and restriction of extraocular muscles were found in type I and II fractures, severe ophthalmic complications such as superior orbital fissure syndrome, orbital apex syndrome, and traumatic optic neuropathy were found in type III and IV fractures almost exclusively. Conclusion: We propose an easy classification system of the lateral orbital wall fracture which correlates closely with ophthalmic complications and may help to make further treatment plan. In Type III and IV fractures, severe ophthalmic complications may ensue in higher rates, so early diagnosis and treatment should be performed.

• ALGAE
• /
• v.33 no.1
• /
• pp.127-141
• /
• 2018

Low efficiency in microalgal biomass production was largely attributed to the low density of algal cell cultures. Though mutations that reduced the level of chlorophyll or pigment content increased efficiency of photon usage and thus the cell-culture density under high-illumination growth conditions (e.g., >$500{\mu}mol\;photon\;m^{-2}\;s^{-1}$), it was unclear whether algae could increase cell-culture density under low-illumination conditions (e.g., ${\sim}50{\mu}mol\;photon\;m^{-2}\;s^{-1}$). To address this question, we performed forward genetic screening in Chlamydomonas reinhardtii. A pool of >1,000 insertional mutants was constructed and subjected to continuous subcultures in shaking flasks under low-illumination conditions. Complexity of restriction fragment length polymorphism (RFLP) pattern in cultures indicated the degree of heterogeneity of mutant populations. We showed that the levels of RFLP complexity decreased when cycles of subculture increased, suggesting that cultures were gradually populated by high cell-culture density (HCD) strains. Analysis of the 3 isolated HCD mutants after 30 cycles of subcultures confirmed that their maximal biomass production was 50-100% higher than that of wild type under low-illumination. Furthermore, levels of chlorophyll content in HCD mutant strains were similar to that of wild type. Inverse polymerase chain reaction analysis identified the locus of insertion in two of three HCD strains. Molecular and transcriptomic analyses suggested that two HCD mutants were a result of the gain-of-function phenotype, both linking to the abnormality of mitochondrial functions. Taken together, our results demonstrate that HCD strains can be obtained through continuous subcultures under low illumination conditions.

• Asian-Australasian Journal of Animal Sciences
• /
• v.28 no.8
• /
• pp.1066-1074
• /
• 2015

In contrast to high genetic diversity of mitochondrial DNA (mtDNA), equine Y chromosome shows extremely low variability, implying limited patrilines in the domesticated horse. In this study, we applied direct sequencing and restriction fragment length polymorphism (RFLP) methods to investigate the polymorphisms of 33 Y chromosome specific loci in 304 Chinese indigenous horses from 13 breeds. Consequently, two Y-single nucleotide polymorphisms (SNPs) (Y-45701/997 and Y-50869) and one Y-indel (Y-45288) were identified. Of those, the Y-50869 (T>A) revealed the highest variation frequency (24.67%), whereas it was only 3.29% and 1.97% in Y-45288 (T/-) and Y-45701/997 (G>T) locus, respectively. These three mutations accounted for 27.96% of the total samples and identified five Y-SNP haplotypes, demonstrating genetic diversity of Y chromosome in Chinese horses. In addition, all the five YSNP haplotypes were shared by different breeds. Among 13 horse breeds analyzed, Balikun horse displayed the highest nucleotide diversity (${\pi}=5.6{\times}10^{-4}$) and haplotype diversity (h = 0.527), while Ningqiang horse showed the lowest nucleotide diversity (${\pi}=0.00000$) and haplotype diversity (h = 0.000). The results also revealed that Chinese horses had a different polymorphic pattern of Y chromosome from European and American horses. In conclusion, Chinese horses revealed genetic diversity of Y chromosome, however more efforts should be made to better understand the domestication and paternal origin of Chinese indigenous horses.

• The Korean Journal of Mycology
• /
• v.33 no.2
• /
• pp.98-102
• /
• 2005

In 2003 to 2005, the root rot of Japanese angelica (Aralia elata) was surveyed in nursery beds of Korea, where incidence of the disease often reached up to 100%. Three isolates were obtained from the infected roots, and identified as Phytophthora cactorum on the basis of cultural, morphological characteristics and molecular analysis. The isolates were characterized by having markedly papillate and broadly ovoid deciduous sporangia. The optimum temperature for mycelium growth was at $25^{\circ}C$ on V8 juice agar. Pathogenicity of the isolates was confirmed by soil mixture inoculation. Approximately 900 bp of ITS rDNA was amplified from all 3 isolates and band pattern of restriction fragments observed by Alu I, Msp I, and Taq I digestion also supported the result of the morphological identification when compared with PhytID database.

• Journal of Hydrogen and New Energy
• /
• v.26 no.3
• /
• pp.241-246
• /
• 2015

The interest of New Renewable Energy is increasing globally because of the increment of the uncertainty for the energy's supply and demand, and the increment of the frequency in weather anomaly and its damages. One of the New Renewable Energies, Hydrogen receives attention as the future energy that can deal with global environment regulation. Fuel Cell Electric Vehicle (FCEV) is an environment-friendly vehicle that uses Hydrogen as fuel. The electric power for FCEV is generated by chemical reaction with Oxygen from the air and Hydrogen. Hyundai Motor Company (HMC) has developed a proprietary fuel cell system since 2005. In 2012, HMC is the first car maker that mass-produces the ix35 FCEV to the worldwide such as North America, Europe, etc. In order to develop and improve the FCEV technology, data acquisition and analysis of the driving vehicle information is essential. Therefore, the monitoring system is developed, which is consist of datalogger, Automatic Vehicle Location (AVL) server and main server. Especially, WCDMA technology is integrated into the system which enables the data analysis without any restriction of time and region. The main function of the system is the analysis of the driving pattern and the component durability, and the safety monitoring. As a result, ix35 FCEV has successfully developed by using the developed monitoring system. The system is going to take an advantage of development in the future FCEV technology.

• Journal of the Korean Society of Costume
• /
• v.53 no.1
• /
• pp.49-66
• /
• 2003

In the relationship between post-modern feminism and the body. the body of women, which has been taken as 'other' by dominant arguments, has escaped from the fixed idea at post-modern times and the post-modern feminists tried to escape from the taste of the times about body image that was prevalent in the former times.'The taste of the times' here means the fixed physical form of ideal women's body by the educational custom, and it includes the educational prescription which establishes the docile body of women. This kind of educational custom aims at the perfect and permanent restriction on size, shape, desire, attitude and movement as well as on the overall demeanor and each part of the body. And the custom produces 'dependent and trained'. that is, inferiorized women's body. Hence, this paper points out that the feminity as a fixed Physical form should be overcome in order to move forward, and as one part of the effort, it attempts to analyze the designs which deformed and distorted women's body in 1990s. This research indicates that the work, which tries to deviate the fantasized and casted women's mystery, has been closely connected with the expression of 'non-separate bodies' and 'irregular body shapes' in 1990s, which aims at escaping from the fixation of women's body Image. Therefore, it is concluded that the target design trend of this research, which expressed the deformation and perversion of the body, provides the anti-these against the ideal body image of women and the these of feminity. which have been reproduced in various methods and patterns throughout the public and the culture on the whole by expressing the refusal and deviation of the existing women's body image through the post-fixation of the body pattern.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.244-244
• /
• 2017

Soil salinity due to accumulation of salts particularly sodium chloride affects agricultural lands and their vegetation. Generally, rice is a moderately sensitive plant with some cultivars with varying tolerance to salinity. Though there are physiological differences between salt-sensitive and salt-tolerant rice cultivars, both are still affected especially during high salinity and prolonged exposure. This also ultimately affects their indigenous bacterial endophytes particularly those that inhabit the rice seed endosphere. This study investigates the dynamic structure of seed bacterial endophytes of salt-sensitive and tolerant rice cultivars grown in different levels of soil salinity. Endophytic bacterial diversity was studied Terminal-Restriction Fragment Length Polymorphism (T-RFLP) analysis. Results revealed a very interesting pattern of diversity and shifts in community structure of bacterial endophytes in the rice seeds. There is a general decrease in diversity for the salt-sensitive rice cultivar, IR29 as soil salinity increases. For the salt-tolerant cultivars, IC32 and IC37, diversity interestingly increased at moderate salinity then decreased at high soil salinity. The patterns of community structure is also strikingly different for the salt-sensitive and salt-tolerant rice cultivars. IR29 has a more even distribution of abundance, but under soil salinity, the community shifted where Curtobacterium, Pantoea, Flavobacterium and Microbacterium become the more dominant bacterial communities. For IC32 and IC37, the dominant bacterial groups under normal stress conditions were also the dominant bacterial groups during salt stress conditions. Their seed bacterial community is dominated by endophytes belonging to Microbacterium, Flavobacterium, Pantoea, Kosakonia and Enterobacter. Stenotrophomonas and Xanthomonas have not changed in terms of abundance under different salinity stress level in the salt-sensitive and salt-tolerant rice cultivars. This study showed that soil salinity greatly influenced the seed bacterial communities of rice seeds irrespective of their physiological tolerance to salinity.

• Explosives and Blasting
• /
• v.26 no.2
• /
• pp.29-37
• /
• 2008

In general, the size of tunnel cross section in construction site is $50{\sim}200m^2$. But, electric cable tunnel, telecommunication cable tunnel, mine tunnel. Waterproof tunnel have small cross section less than $20m^2$. There are so many problem at small sectional tunnel: restriction of equipment, dead pressure by precompression, loss of efficiency, increase of work time. Especially, explosives remainder by precompression of previous detonation is serious problem. To find its measures of dead pressure (explosives remainder), the following series of progress have been conducted: (1) survey of previous study (2) investigate causes of dead pressure (3) set up of its measures (4) application and appraisal at tunnel site. The measures, change of cut pattern, hole space over 40cm, adjustment of delay time, are proved by experimental results.

• KSBB Journal
• /
• v.7 no.3
• /
• pp.191-200
• /
• 1992

Several strains of Agrobacterium tumefaciens were isolated from soil in the Taegu area and characterized to develop some useful vector systems for higher plant genetic engineering. The selected colonies had a unique form, and strains from the colonies were capable of tumor formation on the sunflower leaf surface. They had a large plasmid. The restriction analysis showed that they were another kinds of Ti plasmic compared with C58 and Ach5. The isolated strains were identified as the nopaline type and also as biovar 1 A. tumefaciens, according to their tumor morphology, blophyslcal and biochemical characteristics. One of the isolated strains, AK204 was transformed with binary vector (pGA642), having selectable marker (Kmr, Tcr). Furthermore, maize tissue cells were transformed by cocultivation with AK204/pGA642, and the transformants were selected on the selective medium and identified using PAGE patterns of their soluble proteins.