• Environmental Mutagens and Carcinogens
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• v.18 no.2
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• pp.116-122
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• 1998

The present investigation has been performed to elucidate the effect of pretreatment with low dose of ultraviolet radiation (UV), ethyl methansulfonate (EMS), and bleomycin (BLM) on cell survival and lectin-binding glycoconjugates of plasma membrane in HeLa cells treated with mutagen. The percentage of survival of cells pretreated with 1 mM EMS following treatment with 10 mM EMS was higher than that of cells treated with 10 mM EMS alone. Wheat germ agglutinin (WGA) staining intensity of cells pretreated with 1 mM EMS and subsequently treated with 10 mM EMS was stronger than that of cells treated with 10 mM EMS alone. But, succinylated wheat germ agglutinin (sWGA) staining intensity of cells pretreated with 1 mM EMS and subsequently treated with 10 mM EMS was similar to that of cells treated with 10 mM EMS alone. These results suggest that the acquired resistance to EMS is related to the glycoconjugates containing sialic acid of plasma membrane involved in multidrug resistance or adaptive response in HeLa cells.

• Journal of Korean Neurosurgical Society
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• v.59 no.6
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• pp.570-576
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• 2016

Objective : Elevated cell counts and protein levels in cerebrospinal fluid (CSF) result from disease activity in patients with leptomeningeal carcinomatosis (LMC). Previous studies evaluated the use of CSF profiles to monitor a treatment response or predict prognosis. CSF profiles vary, however, according to the sampling site and the patient's systemic condition. We compared lumbar and ventricular CSF profiles collected before intraventricular chemotherapy for LMC and evaluated the association of these profiles with patients' systemic factors and LMC disease activity. Methods : CSF profiles were retrospectively collected from 228 patients who underwent Ommaya reservoir insertion for intraventricular chemotherapy after a diagnosis of LMC. Lumbar samples taken via lumbar puncture were used for the diagnosis, and ventricular samples were obtained later at the time of Ommaya reservoir insertion. LMC disease activity was defined as the presence of LMC-related symptoms such as increased intracranial pressure, hydrocephalus, cranial neuropathy, and cauda equina syndrome. Results : Cell counts (median : 8 vs. 1 cells/mL) and protein levels (median : 68 vs. 17 mg/dL) significantly higher in lumbar CSF than in ventricular CSF (p<0.001). Among the evaluated systemic factors, concomitant brain metastasis and previous radiation were significantly correlated with higher protein levels in the lumbar CSF (p=0.01 and <0.001, respectively). Among the LMC disease activity, patients presenting with hydrocephalus or cauda equina syndrome showed higher lumbar CSF protein level compared with that in patients without those symptoms (p=0.049 and p<0.001, respectively). The lumbar CSF cell count was significantly lower in patients with cranial neuropathy (p=0.046). The ventricular CSF cell counts and protein levels showed no correlation with LMC symptoms. Carcinoembryonic antigen (CEA), which was measured from ventricular CSF after the diagnosis in 109 patients, showed a significant association with the presence of hydrocephalus (p=0.01). Conclusion : The protein level in lumbar CSF indicated the localized disease activity of hydrocephalus and cauda equina syndrome. In the ventricular CSF, only the CEA level reflected the presence of hydrocephalus. We suggest using more specific biomarkers for the evaluation of ventricular CSF to monitor disease activity and treatment response.

• Journal of Korean Neurosurgical Society
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• v.30 no.3
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• pp.334-341
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• 2001

Objective : We have currently changed treatment strategies to methotrexate(MTX)-based preirradiation chemotherapy with subsequent planned radiation for the initial therapy of primary central nervous system lymphoma (PCNSL). The aim of this study was to evaluate the results of treating PCNSL with chemotherapy plus radiotherapy (CRT) or radiotherapy(RT) alone. Method and Material : This study involved 10 females and 3 males patients with a mean age of 54.2 years. All patients underwent surgery, open(8 cases) or stereotactic biopsy(5 cases) for histological diagnosis. Eleven tumors were diffuse large B-cell lymphomas. Tumor volume change in the follow-up images and survival time were evaluated in patients treated with CRT and RT alone. In the beginning, two patients received ProMACE-Cytabom chemotherapeutic regimen, but did not complete the course and died of progressive tumor 8 and 9 months after diagnosis, respectively. One patient died at 6 months before chemotherapy. These three were excluded from the survival analysis. Five patients(RT group) completed full courses of cranial irradiation with or without boost. For the current combined modality treatment, high-dose MTXbased chemotherapy(systemic and intrathecal MTX, IV vincristine, and oral procarbazine) followed by whole brain irrdiation to 45Gy to tumor was introduced in 5 patients of CRT group. Result : A complete response was achieved in three of five who received RT only and in all of five who received CRT. All patients in CRT groups are in disease free status at a mean 23 months following therapy. The RT group patients refused any additional salvage therapy at tumor relapse and survived at mean 20 months from diagnosis. The Karnofsky performance status improved in eight of ten patients with treatment. The treatment toxicity included leukoencephalopathy in RT group and severe leukopenia, transient hepatitis, avascular necrosis of femoral head, hearing loss, and amenorrhea in CRT group, respectively. Conclusion : The combined modality therapy of MTX-based chemotherapy plus radiotherapy for PCNSL may enhance tumor response and improve patient survival. The patients who received CRT should be carefully followed up because of the higher risk of treatment-induced late neurotoxicity.

• Journal of Life Science
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• v.29 no.11
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• pp.1200-1207
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• 2019

Ultraviolet radiation exposure is a major cause of extrinsic skin aging, which leads to skin hyperpigmentation. Loganin, a major iridoid glycoside obtained from Corni fructus, has anti-inflammatory, anti-diabetic, and neuroprotective effects. In this study, we investigated the mechanisms underlying the anti-melanogenic effects of loganin in B16F10 melanocytes treated with ${\alpha}$-melanocyte stimulating hormone (${\alpha}-MSH$) and 3-isobutyl-1-methylxanthine (IBMX). Anti-melanogenic activity was measured by treating cells with loganin at concentrations between 1 and $20{\mu}m$. Cell viability assays confirmed that doses of loganin up to $20{\mu}m$ were not cytotoxic. Loganin significantly and dose-dependently decreased intracellular melanin production. We also investigated potential molecular signaling pathways for the anti-melanogenesis effects of loganin. Western blotting showed that treatment with ${\alpha}-MSH$ and IBMX increased the phosphorylation of cAMP response element-binding protein (CREB) and the gene expressions of microphthalmia-associated transcription factor (MITF) and tyrosinase. Addition of loganin suppressed these increases, while promoting the phosphorylation of extracellular signal regulated kinase (ERK) and the anti-melanogenesis response. Our data therefore indicated that loganin could attenuate the increased melanin synthesis induced by ${\alpha}-MSH$ and IBMX treatment of B16F10 melanocytes. This attenuation appears to occur by downregulation of CREB phosphorylation and MITF and tyrosinase gene expression and upregulation of ERK phosphorylation. These finding suggests that loganin could be a valuable candidate for treatment of skin diseases related to hyperpigmentation.

• Korean Journal of Agricultural and Forest Meteorology
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• v.25 no.4
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• pp.311-325
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• 2023

This study was conducted to analyze the habitat suitability of the major honey trees including Kalopanax septemlobus Koidz., Prunus spp., Tilia spp., and Styrax obassia Siebold & Zucc. indigenous to mountain Gariwang and Yumeong using the machine learning approach (i.e., MaxEnt model). The AUC values of the model predictions were mostly above 0.7, and the results of the response curves showed that the environmental drivers that had effects on the habitat suitability of the major honey trees were elevation, mean annual precipitation, and mean annual temperature. These results indicate that climatic drivers along the elevation gradient are the main environmental drivers in explaining the distribution patterns of the major honey trees. In addition, the results of the response curves of Prunus spp. and Styrax obassia Siebold & Zucc. differed slightly in terms of slope and mean annual solar radiation as the main environmental drivers. The results of this study will be valuable for the establishment of honey tree forests and management plans for the natural and artificial forests in South Korea, as well as for the mapping the distribution of honey trees. Further studies at different regional levels, reflecting biotic drivers, will be needed to expand the production of honey and pollen at different strata and to produce honey annually.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.23 no.9
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• pp.708-713
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• 2010

In this research a ring light was developed so that a partial shadowless shooting for the patient's affected area at the medical treatment room and surgical operation room using high luminance light emitting diode (LED) for which attention is being paid as new lighting parts for medical purpose. LED which was applied to the development used high luminance three color LED for full color for which various color materialization and the adjustment of radiation intensity are possible and we can get white light in order to emphasize the delicate expression for generic tone of shooting object, strong highlight, simple shadow and three dimensional effect at the time of close-up shadowless shooting of the affected area. And at the time of design of ring light, the characteristics of LED and the loss of light at the time of penetrating light diffusion PC were considered so that intensity of illumination for over 150 lx can be obtained. The result of measurement of the intensity of illumination of the ring light that was developed revealed that maximum intensity of illumination of 225.7 lx was obtained, while smoke index was measured to be maximum 78 Ra in the case of Red(50%) Green(100%) and Blue LED(60%). We could confirm that response speed was also very fast as 1.72 ms.

• Progress in Medical Physics
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• v.2 no.2
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• pp.129-139
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• 1991

The method of etching tracks in PN-3 dosimeter has been applied to tracks of recoil protons from a neutron source. Both the etch and the detection response of PN-3 has been studied as a function of etched-track diameters against various parameters. We could obtain very useful informations about charge, energy, and mass of particles and the relationship between the track etching rate and the track forming procedure in order to analyze the particle recorded in the solid state track detector. The best etching condition could be found by means of changing the etching circumstances for various energies and particles in order to detect the charged particle accurately. It could be influenced widely that the polymer plastic detector could develep the detecting technique for the low energy level neutron and could be used as a neutron dosimeter in the radiation field such as the nuclear power station, the medical institute and the nondtructive testing institute.

• The Bulletin of The Korean Astronomical Society
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• v.39 no.2
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• pp.113.1-113.1
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• 2014

We propose a three-year Key Science Program (KSP) consisting of VLBI monitoring observations and single dish (SD) rapid response observations (RRO). The VLBI monitoring observations are comprised of ten 24-hr observations per year (every month) of about 30 gamma-ray brigt active galactic nuclei (AGNs) with Korea VLBI Network (KVN) at 22, 43, 86, and 129 GHz. The SD RROs may consist of twelve 7-hr observations per source (every week for 3 months after triggering) of gamma-ray flaring sources with two KVN SD telescopes at 22, 43, and 86 GHz in dual polarization. We expect one or two sources per year for the SD RROs. Gamma-ray flares of AGNs are known to be occured in innermost regions of relativistic jets which radiate in whole ranges of electromagnetic spectra due to synchrotron radiation, syschrotron self absorption, inverse-compton scttering, doppler boosting etc. Possible explanations of the gamma-ray flares in AGNs are a) shocks-in-jets propagating within jet flow and b) bending of the whole jets. For both cases, we should expect changes in polarization, luminosity, particle distribution, and structures of jets at mas-scale. The multifrequency simultaneous VLBI/SD observations with KVN are the best tool for detecting such changes correlated with gamma-ray flares. This KSP proposal aims to answer the fundamental questions about the basic nature of the flares of AGNs.

• The Journal of the Acoustical Society of Korea
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• v.37 no.4
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• pp.196-205
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• 2018

This paper presents an algorithm for passive localization of a ship by applying the ray back-propagation technique to the ship radiation noise data. The previous method [S. H. Abadi, D. Rouseff and D. R. Dowling, J. Acoust. Soc. Am. 131, 2599-2610 (2012)] estimates the position of a sound source in the near-field environment with no array tilt by using the RBD (Ray-based Blind Deconvolution) and ray back-propagation techniques. However, when there exists an array tilt, the above method leads to a large position estimation error. In order to overcome the problem, this study proposes an algorithm that estimates the position of a sound source by correcting the array tilt using the RBD and ray back-propagation techniques. The proposed algorithm was verified by using the ship noise of SAVEX15 (Shallow-water Acoustic Variability EXperiment in 2015) experimental data.

• Tuberculosis and Respiratory Diseases
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• v.41 no.4
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• pp.413-417
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• 1994

We experienced one case of combined mixed germ cell tumor and angiosarcoma metastasis to the lung within the mediastinum. He was 19-year-old male, complained of hemoptysis, coughing, purulent sputum and weight loss for 2 months prior to adimission. The patient underwent open thoracotomy, but we couldn't resect the mass completely, because of tumor encroachment on adjacent mediastinal structures. He began radiation therapy involving the right lung but had no response to therapy.