• 한국가축번식학회지
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• 제17권1호
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• pp.49-56
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• 1993

Mouse mammary epithelial cells(NMuMG) were plated onto 24 well phates(100,000 cells/well), in DMEM supplemented with 10% fetal calf serum. After serum starvation for 24 hours, EGF)0~100ng/ml) was added simultaneously with IGF-I(10ng/ml), 1$\mu$M photoreactive cAMP(4,5-dimethoxy-2-nitrobenzyl adenosine-3',5' cyclic monophosphate, DMNB) or IGF-I plus DMNB. After 2 hours, the cells were expposed to UV light(300nm, 3 second pulse0 in order to activate DMNB which induces a rapid transient increase in intracellular cAMP upon UV irradiation. DNA synthesis was estimated as incorporation of 3H-thymidine into DNA(1 hour pulse with 1$\mu$Ci/ml, 18~19 hours after UV exposure). Without IGF-I or DMNB, EGF(10 or 100ng/ml) increased DNA synthesis from 8,362 dpm/well in control to 16,345 or 18,684 dpm/well with EGF(pooled SE=1,239 dpm/well, P<0.05). IGF-I or IGF-I plus DMNB alone increased DNA synthesis from 8,362 dpm/well in control to 17,307 or 20,427 dpm/well, respectively(P<0.05). Addition of IGF-I, DMNB or IGF-I plus DMNB into 0~100ng/ml EGF did not significantly change the shape of dose response curve of EGF alone. In other experiment, EGF or IGF-I plus DMNB into 10ng/ml EGF group exhibited interaction effect in DNAsynthesis [EGF(10ng/ml)=18,497; IGF-I＋EGF=22,837; DMNB＋EGF=20,658 ; IGF-I＋DMNB＋EGF=29,658, pooled SE=1,055, P<0.05]. These results indicate that simultaneous activation of EGF, IGF-I and intracellular cAMP interact in DNA synthesis of mouse mammary epithelial cells.

• Clinical and Experimental Pediatrics
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• 제60권11호
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• pp.353-358
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• 2017

Purpose: To develop and evaluate a simple screening tool to assess hearing loss in newborns. A derived score was compared with the standard clinical practice tool. Methods: This cohort study was designed to screen the hearing of newborns using transiently evoked otoacoustic emission and auditory brain stem response, and to determine the risk factors associated with hearing loss of newborns in 3 tertiary hospitals in Northern Thailand. Data were prospectively collected from November 1, 2010 to May 31, 2012. To develop the risk score, clinical-risk indicators were measured by Poisson risk regression. The regression coefficients were transformed into item scores dividing each regression-coefficient with the smallest coefficient in the model, rounding the number to its nearest integer, and adding up to a total score. Results: Five clinical risk factors (Craniofacial anomaly, Ototoxicity, Birth weight, family history [Relative] of congenital sensorineural hearing loss, and Apgar score) were included in our COBRA score. The screening tool detected, by area under the receiver operating characteristic curve, more than 80% of existing hearing loss. The positive-likelihood ratio of hearing loss in patients with scores of 4, 6, and 8 were 25.21 (95% confidence interval [CI], 14.69-43.26), 58.52 (95% CI, 36.26-94.44), and 51.56 (95% CI, 33.74-78.82), respectively. This result was similar to the standard tool (The Joint Committee on Infant Hearing) of 26.72 (95% CI, 20.59-34.66). Conclusion: A simple screening tool of five predictors provides good prediction indices for newborn hearing loss, which may motivate parents to bring children for further appropriate testing and investigations.

• Clinical and Experimental Pediatrics
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• 제59권10호
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• pp.408-413
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• 2016

Purpose: This study investigated predictors of unresponsiveness to second-line intravenous immunoglobulin (IVIG) treatment for Kawasaki disease (KD). Methods: This was a single-center analysis of the medical records of 588 patients with KD who had been admitted to Asan Medical Center between 2006 and 2014. Related clinical and laboratory data were analyzed by univariate and multivariate logistic regression analyses. Results: Eighty (13.6%) of the 588 patients with KD were unresponsive to the initial IVIG treatment and received a second dose. For these 80 patients, univariate analysis of the laboratory results obtained before administering the second-line IVIG treatment showed that white blood cell count, neutrophil percent, hemoglobin level, platelet count, serum protein level, albumin level, potassium level, and C-reactive protein level were significant predictors. The addition of methyl prednisolone to the second-line regimen was not associated with treatment response (odds ratio [OR], 0.871; 95% confidence interval [CI], 0.216-3.512; P=0.846). Multivariate analysis revealed serum protein level to be the only predictor of unresponsiveness to the second-line treatment (OR, 0.160; 95% CI, 0.028-0.911; P=0.039). Receiver operating characteristic curve analysis to determine predictors of unresponsiveness to the second dose of IVIG showed a sensitivity of 100% and specificity of 72% at a serum protein cutoff level of <7.15 g/dL. Conclusion: The serum protein level of the patient prior to the second dose of IVIG is a significant predictor of unresponsiveness. The addition of methyl prednisolone to the second-line regimen produces no treatment benefit.

• The Korean Journal of Physiology and Pharmacology
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• 제5권5호
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• pp.413-421
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• 2001

An impaired smooth muscle cell (SMC) relaxation of coronary artery by alteration of $K^+$ channels would be the most potential explanation for reduced coronary reserve in left ventricular hypertrophy (LVH), however, this possibility has not been investigated. We performed morphometrical analysis of the coronary artery under electron microscopy and measured $Ca^{2+}-activated\;K\;(K_{Ca})$ currents and delayed rectifier K $(K_{dr})$ currents by whole-cell and inside-out patch-clamp technique in single coronary arterial SMCs from rabbits subjected to isoprenaline-induced cardiac hypertrophy. Coronary arterial SMCs underwent significant changes in ultrastructure. The unitary current amplitude and the open-state probability of $K_{Ca}$ channel were significantly reduced in hypertrophy without open-time and closed-time kinetic. The concentration-response curve of $K_{Ca}$ channel to $Ca^{2+}$ is shifted to the right in hypertrophy. The reduction in the mean single channel current and increase in the open channel noise of $K_{Ca}$ channel by TEA were more sensitive in hypertrophy. $K_{dr}$ current density is significantly reduced in hypertrophy without activation and inactivation kinetics. The sensitivity of $K_{dr}$ current on 4-AP is significantly increased in hypertrophy. This is the first study to report evidence for alterations of $K_{Ca}$ channels and $K_{dr}$ channels in coronary SMCs with LVH. The findings may provide some insight into mechanism of the reduced coronary reserve in LVH.

• 한국산업융합학회 논문집
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• 제23권1호
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• pp.83-91
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• 2020

The evaluation of the compression behavior of the cushioning material is of importance to achieve appropriate packaging design. In order to change packaging design from polymeric-based to more eco-friendly cellulose-based nire effectively, comparative study on the compression behavior between these two packaging materials is crucial. In this study, the stress-strain behavior, hysteresis loss, and response characteristics for cyclic loading were analyzed through compression tests on multi-layered corrugated structure (MLCS) and expanded polystyrene (EPS) packaging materials. MLCS produced in Korea is produced by winding a certain number of single-faced corrugated paperboard, and the compression behavior of this material was turned out to be 6 stages: elastic stage, first buckling stage, sub-buckling stage, densification stage, last buckling stage and high densification stage. On the other hand, EPS's compression behavior was in 3 stages: linear elastic stage, collapse plateau, and densification stage. The strain energy per unit volume (strain energy density) of MLCS did not differ depending on the material thickness, but it showed a clear difference depending on the raw material and flute type. Hysteresis loss of MLCS ranged from 0.90 to 0.93, and there were no significant differences in the raw material and flute type. These values were about 5 to 20% greater than the hysteresis of the EPS (about 0.78 to 0.87).

• Applied Biological Chemistry
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• 제40권3호
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• pp.225-231
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• 1997

임의로 선정되어진 국산두부콩과 수입두부콩들의 물성 및 색도에 대한 최적화이론을 적용하였다. 제조한 두부의 파손강도와 응력완화현상은 rheometer를 사용하여 측정하였으며, 색도는 colorimeter를 이용하였다. 각 두부콩들의 효과는 수식화된 non-linear canonical regression mpdel로 표현하였으며 각 콩들의 기여도는 trace plot으로 나타내었다. 국산콩은 그 첨가량이 증가할수록 두부의 파손강도는 다른 수입콩보다 우월하게 나타나 두부조직의 강도에는 뛰어남을 보여주었으나 두부조직의 점탄성에 있어서는 약간 뒤떨어짐을 보여주었으며 색감에서는 크게 뒤지는 것으로 나타나서 선정된 물성반응에 대하여 선별적인 경쟁력을 가짐을 보여주었다. 그러나 국산콩의 흡습능력은 수율에 결과적으로 영향을 주며 이를 새로운 반응치로 최적화이론에 적용하였고 그 결과 가격경쟁과 반응에 대한 취약점을 보완하여 최적화된 결과에 기여함을 보여주었다. 이와같은 국산두부콩과 수입두부콩의 최적화된 배합비율은 수율과 가격이라는 두 양립되는 목적함수로써 발산함을 보여 주었으며 이를 수렴시키기 위하여서는 가증치도입과 국산콩의 흡습능력조절에 대한 가공공정의 새로운 연구가 필요하며 이에 대한 조절이 품질향상에 기여할 수 있음을 보여 주었다.

• 대한조선학회논문집
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• 제48권4호
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• pp.299-307
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• 2011

The offshore plants such as FPSO are subjected to combination loading of environmental conditions (swell, wave, wind and current). Therefore the fatigue damage is occurred in the operation time because the units encounter the environmental phenomena and the structural configurations are complicated. This paper is a research for frequency domain fatigue analysis of wide-band random loading focused on accuracy of fatigue damage estimation regarding the proposed methods. We selected ideal bi-modal spectrum. And comparison between time-domain fatigue analysis and frequency-domain fatigue analyses are conducted through the fatigue damage ratio. Fatigue damage ratios according to Vanmarcke's bandwidth parameter are founded for wide-band. Considering safety, we recommend that Jiao-Moan and Tovo-Benasciutti methods are optimal way at the fatigue design for wide-band response. But, it is important that these methods based on frequency-domain unstably change the accuracy according to the material parameter of S-N curve. This study will be background and guidance for the new frequency-domain fatigue analysis development in the future.

• Journal of Chest Surgery
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• 제24권7호
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• pp.637-646
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• 1991

Effect of noradrenaline and endothelium on the high K+ or Ach-induced contraction were investigated in the pig myocardial coronary artery. The helical strip of isolated pig myocardial coronary artery was immersed in the Tris-buffered Tyrode`s solution equilibrated with 100% O2 at 37oC and its isometric tension was measured. High K+ and Ach-induced contraction were dose-dependent. By denuding the endothelium, dose-contraction curve of K+ was not shifted significantly to the left and upward, but that of Ach was shifted significantly to same direction 25 mM K+ - and Ach-induced contraction were relaxed by norepinephrine[NE]. NE-induced relaxation was blocked by the pretreatment of propranolol, which was known as b-adrenoceptor blocker. And, phenylephrine known as a-adrenoceptor agonist, and clonidine known as a-adrenoceptor agonist increased the 25mM K+ - induced contraction respectively. Denuding of endothelium did not show any significant effects on NE-induced relaxation and contraction increased by phenylephrine and clonidine. Tyramine increased 25mM K+ - induced contraction further. The contractile response by tyramine on the 25mM K+ - induced contraction was not blocked by the pretreatment of phentolamine, but was partially blocked by the pretreatment of atropine. From the above results, it is suggested that activation of a1-and a2-adrenoceptors induce the contraction, activation of b-adrenoceptors induce the relaxation, and NE-induced relaxation is mainly due to activation of b-adrenoceptors. Also it is suggested that denudation of endothelium did not influence NE-induced relaxation, but influence Ach-induced contraction in the pig myocardial coronary artery.

• 한국수자원학회논문집
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• 제47권3호
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• pp.269-283
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• 2014

본 연구에서는 기상청에서 제공하는 RCP 기후변화시나리오를 이용하여 기후변화와 유역유출특성에 따른 환경영향을 평가하였다. SWAT모형을 이용한 미래 댐 유입량 평가, HEC-ResSim 모형을 이용한 댐 안전성 평가 및 하류 유황분석을 수행하였다. 또한, 기존 수질관측자료를 이용하여 Seasonal-Kendall Test를 통해 수질변화 추세에 대해 분석하였고, SWAT과 HEC-ResSim 모형으로 도출된 방류량 및 지류유출량을 Qual2E모형에 적용하여 미래 수질변화 추세에 대해 분석하였다. 다음과 같은 유역 통합환경검토 기법은 하천환경에 대한 과학적 물환경 관리 체계를 확보하고, 기후변화 등 새로운 환경문제에 선제적으로 대응하기 위한 지침을 마련할 수 있을 것이다.

• Earthquakes and Structures
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• 제18권2호
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• pp.147-162
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• 2020

This paper presents a novel precast energy dissipation shear wall (PEDSW) structure system that using mild steel dampers as dry connectors at the vertical joints to connect adjacent wall panels. Analytical studies are systematically conducted to investigate the seismic performance of the proposed PEDSW under sequence-type ground motions. During earthquake events, earthquake sequences have the potential to cause severe damage to structures and threaten life safety. To date, the damage probability of engineering structures under earthquake sequence has not been included in structural design codes. In this study, numerical simulations on single-story PEDSW are carried out to validate the feasibility and reliability of using mild steel dampers to connect the precast shear walls. The seismic responses of the PEDSW and cast-in-place shear wall (CIPSW) are comparatively studied based on nonlinear time-history analyses, and the effectiveness of the proposed high-rise PEDSW is demonstrated. Next, the foreshock-mainshock-aftershock type earthquake sequences are constructed, and the seismic response and fragility curves of the PEDSW under single mainshock and earthquake sequences are analyzed and compared. Finally, the fragility analysis of PEDSW structure under earthquake sequences is performed. The influences of scaling factor of the aftershocks (foreshocks) to the mainshocks on the fragility of the PEDSW structure under different damage states are investigated. The numerical results reveal that neglecting the effect of earthquake sequence can lead to underestimated seismic responses and fragilities, which may result in unsafe design schemes of PEDSW structures.