• 대한한방내과학회지
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• 제33권1호
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• pp.62-68
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• 2012

Objectives : This study aimed to evaluate the single oral dose toxicity of Seonpyejeongcheon-tang (SJT) in male and female Sprague-Dawley rats. Methods : In this single oral toxicity study, rats were orally administrated in a single dose of 0 or 5,000 mg/kg SJT. There were 7 rats in each group. After single administration, mortality, clinical signs, body weight changes and gross pathological findings were observed for 14 days. Organ weight, clinical chemistry and hematology were tested after 14 days. Results : There was no mortality or other clinical signs for 14 days. There were also no significant differences in body weight, organ weights, hematological and serum chemical parameters between the SJT and control groups. Conclusions : The results obtained in this study suggest that the 50% lethal dose of SJT is over 5,000 mg/kg, so this finding can be expected to provide scientific evidence for the safety of SJT.

• 대한한방내과학회지
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• 제28권4호
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• pp.797-807
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• 2007

Objectives : The purpose of this study was to investigate whether Mahwangyunpye-tang(MYT) significantly affects mucin secretion from airway epithelial cells. Methods : Confluent hamster tracheal surface epithelial (HTSE) cells were metabolically radiolabeled with 3H-glucosamine for 24 hrs and chased for 30 min in the presence of MYT to assess the effect of the agent on 3H-mucin secretion. Total elution profiles of control spent media and treatment sample through Sepharose CL-4B column were analyzed. Effect of MYT on contractility of isolated tracheal smooth muscle was investigated; also investigated was effect of the agent on MUC5AC gene expression in cultured NCI-H292 cells. Possible cytotoxicities of the agent were assessed both by measuring lactate dehydrogenase (LDH) release from HTSE cells and examining the rate of survival and proliferation of NCI-H292 cells. Results : MYT significantly increased mucin secretion from cultured HTSE cells, without significant cytotoxicity. MYT chiefly affected the 'mucin' secretion. MYT inhibited Acetylcholine-induced contraction of isolated tracheal smooth muscle. MYT did not significantly affect the expression levels of MUC 5AC gene in cultured NCI-H292 cells. Conclusions : Based on the above results, it is suggested that MYT increased mucin secretion from cultured HTSE cells without significant cytotoxicity and inhibited contraction of isolated tracheal smooth muscle.

• 대한의용생체공학회:의공학회지
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• 제35권6호
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• pp.177-184
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• 2014

Continuous monitoring of heart rates and respiratory rates for newborns or infants is very important since the abnormal breathing and heart problems can threaten the life of newborns or infants. A noncontact baby monitoring system based on a Doppler radar and an air mattress was designed. The Doppler radar was used to acquire respiratory signals and the air mattress was employed to obtain heart rates. The performance of the designed system was evaluated using a commercialized infant simulator ($Simbaby^{TM}$) and a respiration belt transducer was used to measure respiration rates as a reference. Results for respiratory rates revealed that the correlation coefficients between I-and Q-channel and the respiration belt were 0.84 and 0.91 and the mean ${\pm}$ standard deviations of errors between them were $1.66{\pm}1.92$ (bpm) and $0.88{\pm}1.65$ (bpm). Heart rates showed that the correlation coefficient between air mattress and set value of the simulator was 0.73 and the mean ${\pm}$ standard deviation of errors between them was $1.09{\pm}3.45$ (bpm). These results indicate that the designed system holds the potential as an effective monitoring tool for continuous monitoring heart rates and respiratory rates of newborns or infants.

• 대한한의학회지
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• 제31권1호
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• pp.14-22
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• 2010

Objectives: This study aimed to evaluate any clinical research regarding asthma published by the Korean Oriental Medical Society and its sub-societies for future rigorous clinical research. Methods: Every article relevant to asthma was initially obtained from journals in the Korean Oriental Medical Society and its sub-societies by electronic search at journal web sites or manual search. Journals were limited to those registered by the Korea Research Foundation. From initial findings, two independent reviewers selected clinical articles and these articles were further analyzed separately into predefined criteria according to prospective and retrospective studies. For prospective studies, quality assessment was also conducted. Results: From 99 articles initially obtained, 19 were finally analyzed. 14 were prospective whereas 5 were retrospective. Among the prospective articles, there were no randomized controlled trials and the non-randomized studies had no control groups, with several problems of quality regarding pre-calculated study size and unbiased assessment. None of the retrospective studies described asthma diagnosis objectively and outcome measures were either non-relevant to asthma or non-validated. Conclusions: Well-designed randomized controlled studies for asthma are needed and more rigorous non-randomized controlled studies should be conducted.

• 대한한방내과학회지
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• 제25권4호
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• pp.93-104
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• 2004

Objectives : The aim was to identify the inhibitory effects of Liriopis Tuber on bleomycin-induced lung fibrosis by analysing the changes of imflammatory cell cytokines and SHI(Semiquantitative Histological Index), Materials and Methods : In this study fibrosis prone C57BL/6J mice were used. Control group was treated with blomycin(0.06mg/0.1 ml) by IT(intratracheal) instillation which is a popular method of inducing lung fibrosis and sample group took Liriopis Tuber water extract(38.0mg/10g body weight) orally for 14 days after IT instillation of blomycin. We measured the total and differential count of WBC, $IFN-{\gamma}$ & IL-4 in mice BALF and SHI(Semiquantitative Histological Index) from lung tissues of mice. BALF and lung tissues of mice were taken 14 days after IT instillation of blomycin. Results : In sample group total WBC count, proportion of neutrophil, SHI and IL-4 significantly(p<0.05) decreased, proportion of macrophage significantly(p<0.05) increased and proportion of lymphocyte, $IFN-{\gamma}$ did not decrease significantly. Conclusions : This study suggests that Liriopis Tuber has an inhibitory effects of pulmonary fibrosis by attenuation of inflammation and Th2 immune response. To determine whether this herbal medicine contribute to cure and prophylaxis of pulmonary fibrosis, further studies on the role of $IFN-{\gamma}$ relating to fibrosis are required.

• 대한한방내과학회지
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• 제31권2호
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• pp.201-211
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• 2010

Objectives : Atopic dermatitis is a chronic inflammatory skin disease which is characterized by severe pruritis, erythema, edema, effusion and scabs. The aim of this study was to understand effects of Arctium lappa Linne, which is well known for its efficacy for various skin diseases, on atopic dermatitis Methods : We conducted this experiment using the DNFB-induced NC/Nga mice. After two weeks of applying DNFB to NC/Nga mice, severe symptoms of atopic dermatitis occurred. We divided the mice into three different groups: a control group which was given no treatment at all, a group treated with dexamethasone (1mg/kg), and another group treated with Arctium lappa Linne (300mg/kg). After one week of treatment, results were recorded according to their improvement on skin, itching behavior, IL-4, and INF-$\gamma$ measurement, which is a significant criterion for diagnosing atopic dermatitis. Results : Itching behavior showed significant improvement in the Arctium lappa Linne group. However Arctium lappa Linne failed to reduce IL-4 and INF-$\gamma$ count. Also there was no satisfying improvement on AD-like skin lesions on the rostral back of the NC/Nga mice treated with Arctium lappa Linne. Conclusions : Arctium lappa Linne showed improvement in itching behavior in NC/Nga mice induced by DNFB. Arctium lappa Linne showed neither significant improvement on skin lesions nor in IL-4 and INF-$\gamma$ measurement.

• 대한한방내과학회지
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• 제31권2호
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• pp.242-253
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• 2010

Objectives : The purpose of this study was to examine the anti-inflammatory effects Forsythiae Fructus on LPS-activated RAW264.7 cells. Methods : Statistical significance was examined through measuring MTT, nitric oxide (NO), TNF $\alpha$, IL 6, NOS2 and COX2 of LPS-activated RAW264.7 cells. Results : In the toxicity experiment of FFE, NO significant toxicity was shown on cells in the concentration ranges of 100, 200 and 300 ${\mu}g/ml$. FFE dose dependently decreases 5.49, 25.41, 33.64 % in LPS induced NO production (p<0.05, p<0.001 and p<0.001), however, FFE don't show significant difference in LPS-induced TNF $\alpha$ and IL 6. Furthermore, FFE showed inhibiting tendency against the revelation of NOS2 and COX2 in LPS-activated RAW264.7 cells, dose-dependently according to concentration. Conclusions : These results mean that FFE is effective for anti-inflammation via inhibition of NOS2 and COX2 expression.

• 동의생리병리학회지
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• 제31권4호
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• pp.213-219
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• 2017

Atopic dermatitis (AD) is a common skin disease characterized by chronic and relapsing inflammatory dermatitis with immunological disturbances. Spleen deficiency (脾虛) is one of the major causes of AD, so development of animal model is required for AD research that reflects the pattern identification. The groups that we have used in this study included Senna folium extracts (SFE), 2,4-dinitrochlorobenzene (DNCB), and normal mice. Therefore, the present study was developed to atopic dermatitis mouse model with spleen deficiency in 2,4-dinitrochlorobenzene (DNCB) and senna leaves extracts induced AD in NC/Nga mice. The results demonstrated that senna leaves extract treatment significantly increased the dermatitis clinical score and epidermal thickness in AD-like skin lesions. We also proved beyond doubt that there was occurrence of erythema and skin moisture indices in the senna leaves extract groups. Further, we also found that the level of serum immunoglobulin E (IgE) in the senna leaves extract-treated group was increased. The amount of IL-4, IL-13, $TNF-{\alpha}$ and $TGF-{\beta}$ mRNA determined by real-time PCR was increased remarkably when senna leaves extract groups were treated on dorsal skin. Senna leaves extract groups significantly promoted the number of CD11B+/Gr-1 cell in skin, as well as the number of CD4+/CD8+ cell in dorsal skin compared with control. The review summarizes recent process in our understanding of the immunopathophysiology of spleen deficiency AD and the implications for spleen deficiency mouse models of AD on drug discovery from medical plants.

• 대한한방내과학회지
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• 제26권1호
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• pp.169-181
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• 2005

Background & Objective : Idiopathic Lung Fibrosis(IPF) is chronic fibrotic interstitial pneumonia. The pathogenesis is unclear. Fritillariae Rhizoma is the most commonly used antitussive and expectorant Oriental medicinal herb. The effects of Fritillariae Rhizoma on bleomycin-induced lung fibrosis is here evaluated. Material and Methods : Fritillariae Rhizoma extract was daily given to the normal rats, control(bleomycin) rats and treated(bleomycin and Fritillariae Rhizoma) rats at 9.0 mg per body weight 10g for 14 days. 14 days later, the change in Leukocyte count and percentages were observed, as well as IFN-gamma and 1L-4 in BALF, and the change in Semiqualitative histological index(SHI). Results : Compared to control rats, Fritillariae Rhizoma treated rats showed a lower leukocyte count(P<0.05) lymphocyte, neutrophil percentage, SHI(p<0.05), IFN-gamma and IL-4(p<0.05) in treated rats. Conversely, macrophage percentages went higher(p<0.05) in treated rats. Conclusion : This study supports a role for Fritillariae Rhizoma in reducing the maintaining inflammatory cells and cytokines in rats with bleomycin-induced lung fibrosis and in the reduction of fibrosis tissues. Further research is needed in order to develop an effective treatment for lung fibrosis.

• 혜화의학회지
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• 제17권1호
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• pp.67-74
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• 2008

Objective: The purpose of this research is to examine the effects of Mahaenggamseok-tang-gagambang (MGTG) on CD3+ T cells, CD4+ T cells and CD8+ T cells in ovalbumin (OVA)-induced asthmatic mice. Methods: C57BL/6 mice were injected, inhaled and sprayed with OVA for 12 weeks (four a week) for asthma induction. Two experimental groups were treated with different concentrations of MGTG (400 mg/kg and 200 mg/kg) extract and cyclosporin A (10 mg/kg) for the later 8 weeks. At the end of the experiment, the mice lung was removed and analyzed CD3+ T cells, CD4+ T cells and CD8+ T cells by flow cytometer. Results: Numbers of CD3+ T cells in lung of the MGTG groups (200, 400 mg/kg) were significantly decreased compared with that of control group. Numbers of CD4+ T cells in lung of the MGTG groups (200, 400 mg/kg) were significantly decreased compared with that of control group. Numbers of CD8+ T cells in lung of the MGTG groups (200, 400 mg/kg) were significantly decreased compared with that of control group. Conclusion: The results of this study suggest that MGTG alleviated asthmatic hyperreactiviry through CD4+ and CD8+ T cells. Further study of relative cytokines is expected.