• Bulletin of the Korean Chemical Society
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• v.16 no.9
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• pp.864-869
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• 1995

Investigation of the structures of the gangliosides has proven to be very important in the understanding of their biological roles such as regulation of differentiation and growth of cells. We used nuclear magnetic resonance spectros-copy in order to investigate the structure of GA1. In order to do this, the assignment of spectra is a prerequisite. Since GA1 does not have polar sialic acid, the spectral overlap is severe. In order to solve this problem, we use 2D NMR spectroscopy and heteronuclear 1H/13C correlated spectroscopy in this study. Here, we report the complete assignment of the proton and the carbon spectra of the GA1 in DMSO-d6-D20 (98:2, v/v). These assignments will be useful for interpreting 1H and 13C NMR data from uncharacterized oligosaccharides and for determining the linkage position, the number of sugar rings, and the sequence of new ganglioside. Amide proton in ring Ⅲ shows many interring nOes and has intramolecular hydrogen bonding. This appears to be an important factor in tertiary folding of GA1. Based on this assignment, determination of three dimensional structure of GA1 will be carried out. Studies on the conformational properties of GA1 may lead to a better understanding of the molecular basis of its functions.

• Journal of the Korea Institute of Military Science and Technology
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• v.22 no.5
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• pp.607-615
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• 2019

In this paper, the electromagnetic effects on the discontinuity structures of the frequency selective radome in manufacturing process based on the X-band were analyzed. In order to fabricate a curved radome using a planar frequency selective surface structure, it is assumed that gaps, slanted gaps, pattern damage, and pattern misalignment between FSS patterns, which are discontinuous elements that can occur at the joint surface of the FSS panel. FSS specimens including continuous elements were fabricated and the frequency transmission characteristics were measured in a free space measurement environment. From the measurement results, resonance frequency shift, transmission performance degradation, and bandwidth variations were found to be the largest when the damaged pattern was bonded to the junction of FSS panels.

• Earthquakes and Structures
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• v.3 no.3_4
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• pp.549-561
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• 2012

Many building foundations are embedded, however it is not easy to compact the backfill around the foundation especially for the deeply embedded ones. The soil condition around the embedded foundation may affect the seismic response of a building due to the weak contact between the soil and the foundation. In this paper, the response accelerations in the short-period range and at the period of 1 second (in the long-period range) for a seismic design spectrum specified in the IBC design code were compared considering perfect and poor backfills to investigate the effect of backfill compaction around the embedded foundation. An in-house finite-element software (P3DASS) which has the capability of horizontal pseudo-3D seismic analysis with linear soil layers was used to perform the seismic analyses of the structure-soil system with an embedded foundation. Seismic analyses were carried out with 7 bedrock earthquake records provided by the Pacific Earthquake Engineering Research Center (PEER), scaling the peak ground accelerations to 0.1 g. The results indicate that the poor backfill is not detrimental to the seismic response of a building, if the foundation is not embedded deeply in the soft soil. However, it is necessary to perform the seismic analysis for the structure-soil system embedded deeply in the soft soil to check the seismic resonance due to the soft soil layer beneath the foundation, and to compact the backfill as well as possible.

• The Korean Journal of Food And Nutrition
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• v.34 no.5
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• pp.553-559
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• 2021

The aim of study to investigate the phytochemicals and biological activities the bark of Betula schmidtii. The studies consisted of the solvent extraction, followed by the isolation of phenolic components 1~3 from ethyl acetate-soluble fraction of Betula schmidtii Bark. Their chemical structures were identified as arbutin (1), ρ-coumaric acid (2) and ferulic acid (3) using Ultraviolet-Visible (UV-Vis) Spectrophotometer, Electrospray Ionization Mass Spectrometry (ESI-MS) (negative ion mode), ¹H-Nuclear Magnetic Resonance (NMR), ¹³C-NMR, ¹H-¹H Correlation Spectroscopy (COSY) and ¹H-¹³C Hetero Nuclear Multiple Quantum Correlation (HMQC) spectral data. Compounds 1~3 shows the anti-oxidant effect with IC₅₀ values of 29.74±1.52, 21.32±1.07 and 34.41±1.24 in 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, respectively. Also, compounds 1~3 exhibited mushroom tyrosinase inhibitory activity with IC₅₀ values of 31.14±1.07, 42.54±1.46 and 69.22±1.43 µM, respectively.

• Journal of Applied Biological Chemistry
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• v.64 no.4
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• pp.391-397
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• 2021

Four compounds were isolated from Salvia plebeia aerial parts. Silica gel open column chromatography with a gradient elution system was used to isolate and purify these compounds. Nuclear magnetic resonance spectroscopy and mass spectroscopy were used for structural elucidation and identification, while electronic circular dichroism was used to confirm the absolute configuration. The structures were determined to be 𝛽-sitosterol (1), (-)-1S,5S,8S,10R-1-acetoxy-8-hydroxy-2-oxoeudesman-3,7(11)-dien-8,12-olide (2), ursolic acid (3), and N-methylhydroxylamine (4). Compounds 2 and 4 were isolated for the first time from this plant. Compound 2 was quantitatively analyzed via HPLC/UV. The results showed that the methanol extract of S. plebeia had a higher content of compound 2 (1.20 mg/g) than the ethanol extract (0.55 mg/g). This study could be used as a preliminary step in conducting HPLC/UV analysis of sesquiterpenoids in S. plebeia extract to assess their bioavailability and potency.

• Mass Spectrometry Letters
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• v.9 no.4
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• pp.91-94
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• 2018

Bilin tetrapyrroles are metabolic products of the breakdown of porphyrins within a species. In the case of mammals, these bilins are formed by the catabolism of heme and can be utilized as either biomarkers in disease or as an indicator of human waste contamination. Although a small subset of bilin tandem mass spectrometry reports exist, limited data is available in online databases for their fragmentation. The use of fragmentation data is important for metabolomics analyses to determine the identity of compounds detected within a sample. Therefore, in this study, the fragmentation of bilins generated by positive ion mode electrospray ionization is examined by collision-induced dissociation (CID) as a function of collision energy on an FT-ICR MS. The use of the FT-ICR MS allows for high mass accuracy measurements, and thus the formulas of resultant product ions can be ascertained. Based on our observations, fragmentation behavior for hemin, biliverdin and its dimethyl ester, phycocyanobilin, bilirubin, bilirubin conjugate, mesobilirubin, urobilin, and stercobilin are discussed in the context of the molecular structure and collision energy. This report provides insight into the identification of structures within this class of molecules for untargeted analyses.

• Smart Structures and Systems
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• v.24 no.2
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• pp.257-266
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• 2019

The benefit of data fusion in improving the performance of Higher Order Sliding Mode (HOSM) observer is brought out in this paper. This improvement in the performance of HOSM observer, resulted in the improvement of active vibration control of a piezo actuated structure, when controlled by a Discrete Sliding Mode Controller (DSMC). The structure is embedded with two piezo sensors for measuring the first two vibrating modes. The fused output of sensors is applied to the HOSM observer for generating state estimates, these states generated are applied to the DSMC, designed for the fourth order linear time invariant model of the structure. In the simulation study, the structure is excited at the first and second mode resonance. It is found that better vibration suppression is obtained, when the states generated by the fused output of sensors is applied as controller input, than the vibration suppression obtained by applying the states generated by using individual sensor output. The closed loop performance of DSMC obtained with HOSM observer is compared with the closed loop performance obtained with the conventional observer. Results obtained shows that better vibration suppression is obtained when the states generated by HOSM observer is applied as controller input.

• Elastomers and Composites
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• v.54 no.1
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• pp.14-21
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• 2019

Main-chain/side-chain liquid crystalline polymers (MCSCLCPs) combined with an azobenzene group and a cholesteryl group were synthesized to impart light and temperature sensitivity to the polymer. The polymers were designed with the azobenzene unit as the mesogenic group of the main-chain and various compositions of the azobenzene and cholesteryl units as the mesogenic group of the side-chain. The chemical structures and physical properties of the synthesized polymers were investigated by Fourier transform infrared spectroscopy, proton nuclear magnetic resonance spectroscopy, differential scanning calorimetry, thermogravimetric analysis, polarized optical microscopy, and ultraviolet-visible (UV-Vis) spectroscopy. All the MCSCLCPs were amorphous and exhibited enantiotropic liquid crystal phases; these polymers achieved the nematic phase with increasing content of the azobenzene group and exhibited the cholesteric phase with weak liquid crystallinity as the content of the cholesteryl group was increased. Furthermore, the polymers containing the azobenzene group showed photoisomerization when exposed to UV-Vis light, and the CP-A3C7 and CP-A5C5 polymers exhibited thermochromism in the temperature range of the liquid crystal phase.

• Korean Journal of Biological Psychiatry
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• v.28 no.2
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• pp.23-35
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• 2021

Fear conditioning and extinction, which are adaptive processes to learn and avoid potential threats, have essential roles in the pathophysiology of anxiety disorders. Experimental fear conditioning and extinction have been used to identify the mechanism of fear and anxiety in humans. However, the brain-based mechanisms of fear conditioning and extinction are yet to be established. In the current review, we summarized the results of neuroimaging studies that examined the brain changes-functional activity and structures-regarding fear conditioning or extinction in healthy individuals. The functional activity of the amygdala, insula, anterior cingulate gyrus, ventromedial prefrontal cortex, and hippocampus changed dynamically with both fear conditioning and extinction. This review may provide an up-to-date summary that may broaden our understanding of pathophysiological mechanisms of anxiety disorder. In addition, the brain regions that are involved in the fear conditioning and extinction may be considered as potential treatment targets in the future studies.

• Wind and Structures
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• v.32 no.2
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• pp.105-114
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• 2021

This paper describes a wind tunnel test on a 1:25 scale model of TTU building with several adjustable openings in order to comprehensively study the characteristics of fluctuating internal pressures, especially the phenomenon of the increase in fluctuating internal pressures induced by tangential flow over building openings and the mechanism causing that. The effects of several factors, such as wind angle, turbulence intensity, opening location, opening size, opening shape and background porosity on the fluctuating internal pressures at oblique wind angles are also described. It has been found that there is a large increase in the fluctuating internal pressures at certain oblique wind angles (typically around 60° to 80°). These fluctuations are greater than those produced by the flow normal to the opening when the turbulence intensity is low. It is demonstrated that the internal pressure resonances induced by the external pressure fluctuations emanating from flapping shear layers on the sidewall downstream of the windward corner are responsible for the increase in the fluctuating internal pressures. Furthermore, the test results show that apart from the opening shape, all the other factors influence the fluctuating internal pressures and the internal pressure resonances at oblique wind angles to varying degrees.