Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Phytochemical constituents from the aerial parts of Salvia plebeia

  • Paje, Leo Adrianne (Department of Plant Science and Technology, Chung-Ang University) ;
  • Lee, Hak-Dong (Department of Plant Science and Technology, Chung-Ang University) ;
  • Choi, Jungwon (Department of Plant Science and Technology, Chung-Ang University) ;
  • Kim, Juree (Department of Plant Science and Technology, Chung-Ang University) ;
  • Kim, Ki Hyun (School of Pharmacy, Sungkyunkwan University) ;
  • Yu, A Ram (Department of Plant Science and Technology, Chung-Ang University) ;
  • Bae, Min-Jung (Technical Assistance Department, The Food Industry Promotional Agency of Korea) ;
  • Lee, Sanghyun (Department of Plant Science and Technology, Chung-Ang University)
  • Received : 2021.09.04
  • Accepted : 2021.10.18
  • Published : 2021.12.31
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Abstract

Four compounds were isolated from Salvia plebeia aerial parts. Silica gel open column chromatography with a gradient elution system was used to isolate and purify these compounds. Nuclear magnetic resonance spectroscopy and mass spectroscopy were used for structural elucidation and identification, while electronic circular dichroism was used to confirm the absolute configuration. The structures were determined to be 𝛽-sitosterol (1), (-)-1S,5S,8S,10R-1-acetoxy-8-hydroxy-2-oxoeudesman-3,7(11)-dien-8,12-olide (2), ursolic acid (3), and N-methylhydroxylamine (4). Compounds 2 and 4 were isolated for the first time from this plant. Compound 2 was quantitatively analyzed via HPLC/UV. The results showed that the methanol extract of S. plebeia had a higher content of compound 2 (1.20 mg/g) than the ethanol extract (0.55 mg/g). This study could be used as a preliminary step in conducting HPLC/UV analysis of sesquiterpenoids in S. plebeia extract to assess their bioavailability and potency.

Keywords

Acknowledgement

This research was financially supported by the Ministry of Trade, Industry and Energy (MOTIE) and Korea Institute for Advancement of Technology (KIAT) through the National Innovation Cluster R&D program (P0006892 Crop Growth Information Quantification System and Commercialization of Big Data Services.

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