• Horticultural Science & Technology
• /
• v.29 no.4
• /
• pp.345-351
• /
• 2011

This study was carried out to develop a model system using selection method for disease resistant plant breeding programs using a herbicide bialaphos-resistant patII gene as a gene-based marker. Spraying bialaphos could eliminate the susceptible plants from the segregating populations such as ${F_2}^{\prime}s$ and thereafter. Tomato cv. Momotaro-yoke was transformed with patII gene 60 independent transformants were acquired. Total 42 transformants were analyzed in transgene copy numbers by Southern blotting and the segregation ratios for the bialaphos resistance. Statistical analysis revealed that the transgene copy numbers and the segregation ratios were not always coincided, especially having the tendency of underestimating the real numbers of the transgenes in the multicopy lines. A two-stepwise screening method was applied to select $T_1$ tomato plants which linked the transgenic patII to a disease resistance gene (I2 and Ve). Based on the resistant to susceptible ratios, T-20 plant was finally selected due to the estimated linkage 12-13 cM between the patII gene to the I2 gene on chromosome 11. This newly developed system could be applied to any economical crop in breeding programs.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2018.10a
• /
• pp.73-73
• /
• 2018

Bacterial leaf blight(BLB), caused by X. oryzae pv. oryzae(Xoo), is one of the most destructive diseases of rice due to its high epidemic potential. Understanding BLB resistance at a genetic level is important to further improve the rice breeding that provides one of the best approaches to control BLB disease. In the present investigation, a collection of 192 accessions was used in the genome-wide association study (GWAS) for BLB resistance loci against four Korean races of Xoo that were represented by the prevailing BLB isolates under Xoo differential system. A total of 192 accessions of rice germplasm were selected on the basis of the bioassay using four isolated races of Xoo such as K1, K2, K3 and K3a. The selected accessions was used to prepare 384-plex genotyping by sequencing (GBS) libraries and Illumina HiSeq 2000 paired- end read was used for GBS sequencing. GWAS was conducted using T ASSEL 5.0. The T ASSEL program uses a mixed linear model (MLM). T he results of the bioassay using a selected set of 192 accessions showed that a large number of accessions (93.75%) were resistant to K1 race, while the least number of accessions (34.37%) resisted K3a race. For races K2 and K3, the resistant germplasm proportion remained between 66.67 to 70.83%. T he genotypic data produced SNP matrix for a total of 293,379 SNPs. After imputation the missing data was removed, which exhibited 34,724 SNPs for association analysis. GWAS results showed strong signals of association at a threshold of [-log10(P-value)] more than5 (K1 and K2) and more than4 (K3 and K3a) for nine of the 39 SNPs, which are plausible candidate loci of resistance genes. T hese SNP loci were positioned on rice chromosome 2, 9, and 11 for K1 and K2 races, whereas on chromosome 4, 6, 11, and 12 for K3 and K3a races. The significant loci detected have also been illustrated, NBS-LRR type disease resistance protein, SNARE domain containing protein, Histone deacetylase 19, NADP-dependent oxidoreductase, and other expressed and unknown proteins. Our results provide a better understanding of the distribution of genetic variation of BLB resistance to Korean pathogen races and breeding of resistant rice.

• Journal of the Korean GEO-environmental Society
• /
• v.19 no.10
• /
• pp.35-41
• /
• 2018

Dynamic interaction of the ground-foundation-structure must be considered for safety of earthquake resistant design for piles supported structures. The p-y curve, which is proposed in the static load and cyclic load cases, is used for the earthquake resistant design of piles. The p-y curve does not consider dynamic interaction of the ground-foundation-structure on dynamic load cases such as earthquake. Therefore, it is difficult to apply the p-y curve to earthquake resistant design. The dynamic p-y curve by considering dynamic interaction of the ground-foundation-structure has been studied, and researches had same conditions that pile caps were on the ground surface and superstructures were added on pile caps for the simple weight. However, group piles are normally embedded into the ground except for marine structures, so it seems that the embedding the pile cap influences on the dynamic p-y curve of group piles. In this study, the shaking table model test was conducted to confirm dynamic behavior of group piles by the embedded pile cap in the ground. The result showed that dynamic behavior was different between two cases by embedding the pile cap or not.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2019.04a
• /
• pp.62-62
• /
• 2019

Bacterial leaf blight(BLB), caused by X. oryzae pv. oryzae(Xoo), is one of the most destructive diseases of rice due to its high epidemic potential. Understanding BLB resistance at a genetic level is important to further improve the rice breeding that provides one of the best approaches to control BLB disease. In the present investigation, a collection of 192 accessions was used in the genome-wide association study (GWAS) for BLB resistance loci against four Korean races of Xoo that were represented by the prevailing BLB isolates under Xoo differential system. A total of 192 accessions of rice germplasm were selected on the basis of the bioassay using four isolated races of Xoo such as K1 and K2. The selected accessions was used to prepare 384-plex genotyping by sequencing (GBS) libraries and Illumina HiSeq 2000 pairedend read was used for GBS sequencing. GWAS was conducted using TASSEL 5.0. The TASSEL program uses a mixed linear model (MLM). The results of the bioassay using a selected set of 192 accessions showed that a large number of accessions (93.75%) were resistant to K1 race and K2 resistant germplasm proportion remained between 66.67. The genotypic data produced SNP matrix for a total of 293,379 SNPs. After imputation the missing data was removed, which exhibited 34,724 SNPs for association analysis. GWAS results showed strong signals of association at a threshold of [-log10(P-value)] more than 5 (K1 and K2) for nine of the 39 SNPs, which are plausible candidate loci of resistance genes. These SNP loci were positioned on rice chromosome 2, 9, and 11 for K1 and K2 races. The significant loci detected have also been illustrated and make the CPAS markers for NBS-LRR type disease resistance protein, SNARE domain containing protein, Histone deacetylase 19, NADP-dependent oxidoreductase, and other expressed and unknown proteins. Our results provide a better understanding of the distribution of genetic variation of BLB resistance to Korean pathogen races and breeding of resistant rice.

• Journal of the Computational Structural Engineering Institute of Korea
• /
• v.37 no.2
• /
• pp.103-110
• /
• 2024

In this study, a nonlinear dynamic analysis of a frame and single member, which reflect the characteristics of a plant facility, is performed using the commercial MIDAS GEN program and the results are analyzed. The general structural members and material properties of the plant are considered. The Newmark average-acceleration numerical-analysis method is applied to a plastic hinge to study material nonlinearity. The blast load of a vapor-cloud explosion, a representative plant explosion, is calculated, and nonlinear dynamic analysis is conducted on a frame and single member. The observed dynamic behavior is organized according to the ratio of natural period to load duration, maximum displacement, ductility, and rotation angle. The conditions and range under which the frame functions as a single member are analyzed and derived. NSFF with a beam-column stiffness ratio of 0.5 and ductility of 2.0 or more can be simplified and analyzed as FFC, whereas NSPF with a beam-column stiffness ratio of 0.5 and ductility of 1.5 or more can be simplified and analyzed as FPC. The results of this study can serve as guidelines for the blast-resistant design of plant facilities.

• Advances in environmental research
• /
• v.3 no.1
• /
• pp.29-43
• /
• 2014

Pharmaceutical wastewater effluents are well known for their difficult elimination by traditional biotreatment methods and their important contribution to environmental pollution due to its fluctuating and recalcitrant nature. OTC is one of the nonbiodegradable antibiotics that makes antibiotic-resistant, so it can make be high risk for environment. NZVI can be a good choice for removal of OTC in aqueous solution. Response surface methodology (RSM) was used to optimize the amounts of NZVI and OTC to be used at pH 3 and under 200 W, UV-A irradiation. The responses were removal percent of absorption at 290 and 348 nm, TOC and COD of OTC. In the optimum condition, Linear model was performed 155 ppm of OTC were removed by 1000 ppm NZVI after 6.5 hours and the removal efficiency of absorption at 290 and 348 nm, TOC and COD were 87, 95, 85 and 89 percent, respectively. In the similar process, there is no organic compound after 14 hours. The parameters ORP, DO and pH were investigated for 6:30 hours to study the type of NZVI reaction in process. In the beginning of reaction, oxidation was the dominant reaction after 3 hours, photocatalytic reaction was remarkable. The mechanism of OTC degradation is proposed by HPLC/ESI-MS and four by products were found. Also the rate constants (first order kinetic chain reaction model) were 0.0099, 0.0021, 0.0010, 0.0049 and $0.0074min^{-1}$, respectively.

• Structural Engineering and Mechanics
• /
• v.64 no.1
• /
• pp.109-120
• /
• 2017

The behavior of moment resistant steel structures depends on both the beam-column connections and columns foundations connections. Obviously, if the connections can meet the adequate ductility and resistance against lateral loads, the seismic capacity of these structures will be linked practically to the performance of these connections. The shape memory alloys (SMAs) have been most recently used as a means of energy dissipation in buildings. The main approach adopted by researchers in the use of such alloys is firstly bracing, and secondly connecting the beams to columns. Additionally, the behavior of these alloys is modeled in software applications rarely involving equivalent torsional springs and column-foundation connections. This paper attempts to introduce the shape memory alloys and their applications in steel structural connections, proposing a new steel column-foundation connection, not merely a theoretical model but practically a realistic and applicable model in structures. Moreover, it entails the same functionality as macro modeling software based on real behavior, which can use different materials to establish a connection between the columns and foundations. In this paper, the suggested steel column-foundation connection was introduced. Moreover, exploring the seismic dynamic behavior under cyclic loading protocols and the famous earthquake records with different materials such as steel and interconnection equipment by superelastic shape memory alloys have been investigated. Then, the results were compared to demonstrate that such connections are ideal against the seismic behavior and energy dissipation.

• Environmental Engineering Research
• /
• v.25 no.2
• /
• pp.205-211
• /
• 2020

Chlorhexidine digluconate (CHD) in the aquatic environment causes irreversible change to microbes, making them resistant to biodegradation, which needs remediation other than biological process. Adsorption study was performed for the removal of CHD on fly ash (FA) as a function of pH and ionic strength. Experimental result has been validated by characterization using Scanning electron microscopy, Fourier Transform-Infrared Spectroscopy and Brunauer-Emmett-Teller. CHD adsorption with FA showed an increasing trend with an increase in pH. Variation in pH proved to be an influential parameter for the surface charge of adsorbent and the degree of ionization of the CHD molecules. The adsorption capacity of CHD decreased from 23.60 mg g-1 to 1.13 mg g-1, when ionic strength increased from to M. The adsorption isotherms were simulated well by the Freundlich isotherm model having R2 = 0.98. The Lagergren's model was incorporated to predict the system kinetics, while the mechanistic study was better explained by pseudo-second order for FA. On the basis of operational conditions and cost-effectiveness FA was found to be more economical as an adsorbent for the adsorption of CHD.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.35 no.4B
• /
• pp.637-646
• /
• 2010

For 40 Gb/s data transmission through electrical backplane trace up to 40 inch length on four layer fire-resistant (FR-4), we have designed the 40 Gb/s backplane channel model consisting of four channel 10 Gb/s. Simulation results show an enhancement of backplane channel characteristics excellent more than requirements specified in IEEE P802.3ba at 10 Gb/s. This paper provides a review of the structures and algorithms used in receive and adaptive equalization for 40 Gb/s backplane Ethernet. The use of this backplane channel model could achieves better receive equalizer at great data rate than 10 Gb/s.

• Journal of the Korean Institute of Telematics and Electronics C
• /
• v.36C no.9
• /
• pp.66-75
• /
• 1999

Image segmentation is the process where an image is segmented into regions that are set of homogeneous pixels. The result has a ciritical effect on accuracy of image understanding. In this paper, an Markov random field (MRF) image segmentation is proposed using genetic algorithm(GA). We model an image using MRF which is resistant to noise and blurring. While MRF based methods are robust to degradation, these require accurate parameter estimation. So GA is used as a segmentation algorithm which is effective at dealing with combinatorial problems. The efficiency of the proposed method is shown by experimental results with real images and application to automatic vehicle extraction system.