• Title/Summary/Keyword: Resistant bacteria

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Comparison of Radiosensitivity of Bacteria Isolated from Given Radiation Exposure History (방사선 피폭역을 달리하여 분리한 세균의 방사선감수성 비교)

  • 김기수;민봉희;이강순
    • Korean Journal of Microbiology
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    • v.12 no.2
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    • pp.67-76
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    • 1974
  • This experiment was carried out to identify and to compare the radiosensitivities of bacteriz isolated from the sources of different radiation exposure histories. Among 10 strains isolated in this investigation, 4 strains of bacteria, Bacillus firmus, Bacillus brevis, Baciilus subtilis and Bacillus sphaericus were isolated from high and low radioactive sites simulaneously. Bacterial strains isolated from radioactive sources such as reactor and isotope production rooms were more resistant to irradiation than the microganisms from medical products and laboratories, however, there was no significance in radiosensitivity in the same species of bacteriz, even if they were isolated from different radiation exposure histories.

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Monitoring of Bacteria using PCR Method and Inactivation with Ozone and UV (PCR법에 의한 박테리아의 동정 및 오존과 UV에 의한 제거 연구)

  • Oh, Byung-Soo;Ju, Seul;Kim, Kyoung-Suk;Kang, Tae-Hee;Lee, Ji-Young;Lee, Hye-Young;Kang, Joon-Wun
    • Journal of Korean Society on Water Environment
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    • v.20 no.2
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    • pp.170-175
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    • 2004
  • The purpose of this study was to monitor bacteria present in raw water and to investigate the effect of ozone, UV and combined ozone/UV processes for inactivating bacteria. Both polymerase chain reaction (PCR)-fragment length polymorphic analysis (PRA) and PCR-sequence analysis (PSA) were applied for the simultaneous analysis of numerous bacteria species present in each tested water, such as drinking water (DRW), drinking water source (DRWS) and sewage effluent water (SEW). According to the result, the number of detected bacteria species was zero in DRW, 58 in DRWS and 13 in SEW. After treatment of the each process, the ozone/UV process was the most successful for inactivating almost all bacteria. However, it was found that Flavobacterium sp., Pseudomonas sp. and Beta proteobacterium sp. had strong resistant to all tested processes, requiring further detailed study.

Disease Suppressive Mechanisms of Antagonistic Bacteria against Phytophthorn capsici causing Phytophthora Blight of Pepper

  • Kim, Hye-Sook;Kim, Ki-Deok
    • Proceedings of the Korean Society of Plant Pathology Conference
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    • 2003.10a
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    • pp.93.2-94
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    • 2003
  • In our previous studies, we selected three antagonistic bacteria, KJ1R5, KJ2C12, and KJ9C8 against Phytophthora capsici, the casual agent of Phytophthora blight of pepper. For elucidating production, root colonization, and total microbial activity were investigated. The dual culture assay was accomplished to elucidate existence of antibiotics. In this assay, any antagonistic bacteria did not inhibit growth of six important fungal plant pathogens, suggesting that these antagonists do not produce antibiotics. root surface or rhizosphere soil colonizations were examined with spontaneous rifampicin-resistant mutants equal to antagonistic ability of wild types. KJ2C12 colonized consistently rhizosphere soil while yellowish colonies of KJ1R5 and KJ9C8 well colonized root surfaces and rhizosphere soil. Total microbial activity in pots treated with the antagonistic bacteria was measured using fluorescein diacetate hydrolysis. total microbial activity of three antagonistic bacteria treatments was significantly higher than that of buffer-treated control until 4days after treatment. However, total microbial activity of treatment of three antagonistic bacteria decreased after 7 days. These results indicate that the antagonistic bacteria, KJ1R5 and KJ9C8 colonized and protected roots well against Phytophthora blight of pepper through competition of infection courts, especially competitions.

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Identification of Bacteriocin-producing Lactic Acid Bacteria from Kimchi and Partial Characterization of their Bacteriocin

  • Ha, Duk-Mo;Cha, Dong-Soo
    • Journal of Microbiology and Biotechnology
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    • v.4 no.4
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    • pp.305-315
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    • 1994
  • Nineteen strains of bacteriocin-producing lactic acid bacteria were isolated from 432 Kimchi samples, and identified by the comprehensive biochemical and morphological tests verifying their cellular fatty acid composition. Using partially purified bacteriocins from these isolates, their inhibitory activities against other lactic acid bacteria and some pathogens, and sensitivity to enzyme and heat treatments were tested. The isolates were identified as Lactobacillus plantarum (2 strains), L curvatus (2 starins), L brevis (2 strains), Enterococcus faecium (6 strains), Leuconostoc mesenteroides subsp. mesenteroides (1 strain) and Lactobacillus sp. (6 strains). The bacteriocins produced by E. faecium strains provided the broadest spectrum of inhibition, affecting against other Gram-positive bacteria including lactic acid bacteria and health-threatening bacteria such as Clostridium perfringens and Listeria monocytogenes. The bacteriocins of Lactobacillus sp., L plantarum and L brevis strains were capable of inhibiting many strains of the lactic acid bacteria, whereas those of L curvatus and L mesenteroides subsp. mesenteroides strains were only inhibitory to a few strains. Generally, the inhibitory activities of both E. faecium and Lactobacillus sp. strains were greater than those of other producer strains. The bacteriocins from the isolates were sensitive to several proteolytic enzymes, and those of L curvatus and L mesenteroides subsp. mesenteroides were also sensitive to lipase and $\alpha$-amylase as well as to proteolytic enzymes. The bacteriocins from the strains of Lactobacillus sp. and a strain of L. brevis were resistant to autoclaving.

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Assessment of the ozonation against pathogenic bacteria in the effluent of the quarantine station

  • Park, Seon Yeong;Kim, Joo Han;Kim, Chang Gyun
    • Journal of Marine Bioscience and Biotechnology
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    • v.13 no.1
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    • pp.10-19
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    • 2021
  • This study investigated how ozone treatment can successfully inactivate pathogenic bacteria in both artificial seawater and effluents discharged from the fishery quarantine station in Pyeongtaek Port, Korea. Vibrio sp. and Streptococcus sp. were initially inoculated into the artificial seawater. All microbes were almost completely inactivated within 10 min and 30 min by injecting 6.4 mg/min and 2.0 mg/min of ozone, respectively. It was discovered that the water storing Pleuronichthys, Pelteobagrus, and Cyprinus imported from China contained the indicator bacteria, Vibrio sp., Enterococcus sp., total coliforms, and heterotrophic microorganisms. Compared to the control, three indicator bacteria were detected at two to six times higher concentrations. The water samples displayed a diverse microbial community, comprising the following four phyla: Bacteroidetes, Proteobacteria, Firmicutes, and Actinobacteria. Almost all indicator bacteria were inactivated in 5 min at 2.0 mg/min of ozonation; comparatively, 92.9%-98.2% of the less heterotrophic microorganisms were deactivated within the same time period. By increasing the dosage to 6.4 mg/min, 100% deactivation was achieved after 10 min. Despite the almost complete inactivation of most indicator bacteria at high doses after 10 min, several bacterial strains belonging to the Proteobacteria have still been found to be resistant under the given operational conditions.

Public Health Risks: Antibiotic Resistance - Review -

  • Barton, Mary D;Hart, Wendy S
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.3
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    • pp.414-422
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    • 2001
  • Antibiotic resistance in human pathogens is a major public health issue. Some of the resistance problem can be attributed to the transfer of resistant bacteria from animals to people and the transfer of resistance genes from animal pathogens and commensal bacteria to human pathogens. Control measures include improvements in food hygiene to reduce the spread of zoonotic bacteria to people via the food chain. However, to specifically address the issue, the medical profession must control misuse and overuse of antibiotics in hospitals and community practice. In addition, the livestock industries and their advisors must reduce and refine the use of antibiotics in animal production and replace antibiotics with alternative disease control measures as much as possible.

Allyl Alcohol Found in Heated Garlic is a Potent Selective Inhibitor of Yeasts

  • Lee Se-Hi;Woo Yong-Ho;Kyung Kyu-Hang
    • Journal of Microbiology and Biotechnology
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    • v.16 no.8
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    • pp.1236-1239
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    • 2006
  • Allyl alcohol (2-propen-l-ol), found in considerable amounts in heated garlic, was able to discriminate yeasts from bacteria and was approximately three orders of magnitude more inhibitory towards yeasts than bacteria. The average minimum inhibitory concentration (MIC) of allyl alcohol for bacteria and yeasts was 5.0% and 0.0056%, respectively. The unsaturated primary alcohols, including allyl alcohol and 2-buten-l-ol, seemed to work differently from all the other saturated alcohols and unsaturated secondary alcohols in inhibiting various yeasts. An alcohol dehydrogenase-negative (ADH$^-$) strain of Saccharomyces cerevisiae was as resistant to allyl alcohol as various bacteria, exhibiting an MIC of 5.0%. The unsaturated primary alcohols were apparently oxidized into the corresponding unsaturated aldehydes before they inhibited the yeasts.

Survival and Thermal Inactivation of Coliform Bacteria after the Heat Treatment at 143°F (유우원(乳牛源) 대장균군세균(大腸菌群細菌)의 치사열(致死熱)에 관한 연구)

  • Chung, G.T.;Han, H.R.
    • Korean Journal of Veterinary Research
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    • v.11 no.2
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    • pp.137-140
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    • 1971
  • Survival and thermal inactivation after heat treatment at $143^{\circ}F$ were observed among 27 strains of coliform bacteria isolated from dairy cattle. The results obtained were as follows. 1. The obvious differences in heat-sensitivity were observed among the strains tested. 2. No strain was found resistant to the heat treatment of $143^{\circ}F$ for 30 minutes. 3. A marked effect of density of coliform bacteria on the survival after the heat treatment was observed. As the density of coliform bacteria was increased, the rate of survival was increased markedly regardless of the length of heat treatment.

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Antibiotic sensitivity and resistance of bacteria from odontogenic maxillofacial abscesses

  • Kang, Sang-Hoon;Kim, Moon-Key
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.45 no.6
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    • pp.324-331
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    • 2019
  • Objectives: This study investigated the types and antibiotic sensitivity of bacteria in odontogenic abscesses. Materials and Methods: Pus specimens from 1,772 patients were collected from affected areas during incision and drainage, and bacterial cultures and antibiotic sensitivity tests were performed. The number of antibiotic-resistant bacteria was analyzed relative to the total number of bacteria that were tested for antibiotic susceptibility. Results: Bacterial cultures from 1,772 patients showed a total of 2,489 bacterial species, 2,101 gram-positive and 388 gram-negative. For penicillin G susceptibility tests, 2 out of 31 Staphylococcus aureus strains tested showed sensitivity and 29 showed resistance. For ampicillin susceptibility tests, all 11 S. aureus strains tested showed resistance. In ampicillin susceptibility tests, 46 out of 50 Klebsiella pneumoniae subsp. pneumoniae strains tested showed resistance. Conclusion: When treating odontogenic maxillofacial abscesses, it is appropriate to use antibiotics other than penicillin G and ampicillin as the first-line treatment.

Population of Pesticide Resistant Strains in Cultivated Soils of Honam Area and Degradation of Chlorothalonil in soil (호남지역 농경지 토양에 분포된 농약내성균의 밀도와 살균제 Chlorothalonil의 분해)

  • Lee, Sang-Bok;Choi, Yoon-Hee
    • Korean Journal of Soil Science and Fertilizer
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    • v.34 no.3
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    • pp.185-191
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    • 2001
  • To obtain the basic information for degradation of remaining pesticide accumulated in cultivated soil of Honam area, the resistant bacterial strains were investigated in Chlorothalonil(TPN). Mancozeb, Bentazone, and Butachlor levels of 100, $500{\mu}g\;ml^{-1}$, and degradation of TPN by TPN-resistant bacteria in sterilized soil was studied under TPN levels 0, 10, 50 and $100{\mu}g\;g^{-1}$. A number of resistance strains were decreased with higher at concentration level of pesticide, and were higher in greenhouse than upland or paddy soil. The resistance of bacteria was strong in other of Bentazone> Butachlor> TPN> Mancozeb. The percentage of bacterial strains of resistance for pesticides isolated from the cultivated soil were the highest in Acinetobacter spp. and Corynebacterium spp., and the lowest in Moraxella spp. A number of TPN-resistant strains were the highest at the TPN level of $10{\mu}g\;g^{-1}$, and 5 days after strains inoculation, and were higher in Pseudomonas spp. TD-25 than TC-23 or strains in non-sterilized soil. The degradation of TPN was fast in order of strain TD-25>strain in non-sterilized soil >TC-23.

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