Journal of Korean Society on Water Environment (한국물환경학회지)

Korean Society on Water Environment (한국물환경학회)
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Monitoring of Bacteria using PCR Method and Inactivation with Ozone and UV

PCR법에 의한 박테리아의 동정 및 오존과 UV에 의한 제거 연구

  • Oh, Byung-Soo (Department of Environmental Engineering, Yonsei University) ;
  • Ju, Seul (Department of Environmental Engineering, Yonsei University) ;
  • Kim, Kyoung-Suk (Department of Environmental Engineering, Yonsei University) ;
  • Kang, Tae-Hee (Department of Environmental Engineering, Yonsei University) ;
  • Lee, Ji-Young (Department of Clinical pathology, Yonsei University) ;
  • Lee, Hye-Young (Department of Clinical pathology, Yonsei University) ;
  • Kang, Joon-Wun (Department of Environmental Engineering, Yonsei University)
  • 오병수 (연세대학교 환경공학과) ;
  • 주설 (연세대학교 환경공학과) ;
  • 김경숙 (연세대학교 환경공학과) ;
  • 강태희 (연세대학교 환경공학과) ;
  • 이지영 (연세대학교 임상병리학과) ;
  • 이혜영 (연세대학교 임상병리학과) ;
  • 강준원 (연세대학교 환경공학과)
  • Published : 2004.03.30
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Abstract

The purpose of this study was to monitor bacteria present in raw water and to investigate the effect of ozone, UV and combined ozone/UV processes for inactivating bacteria. Both polymerase chain reaction (PCR)-fragment length polymorphic analysis (PRA) and PCR-sequence analysis (PSA) were applied for the simultaneous analysis of numerous bacteria species present in each tested water, such as drinking water (DRW), drinking water source (DRWS) and sewage effluent water (SEW). According to the result, the number of detected bacteria species was zero in DRW, 58 in DRWS and 13 in SEW. After treatment of the each process, the ozone/UV process was the most successful for inactivating almost all bacteria. However, it was found that Flavobacterium sp., Pseudomonas sp. and Beta proteobacterium sp. had strong resistant to all tested processes, requiring further detailed study.

Keywords

References

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