• Journal of Food Hygiene and Safety
• /
• v.5 no.4
• /
• pp.197-204
• /
• 1990

This study was carried out to determine the sulfametazine residues in swine tissues. For this experiment, 22 samples of porks were collected at meat markets in Seoul, 21 samples of swine muscles for export were collected at slaughterhouses in Gyunggi areas, and 18 samples of swine livers, kidneys, and muscles were collected from 6 swine at slaugterhouse in Seoul from the end of August to the early of October and were analyzed by High Performance Liquid Chromatography (HPLC). the results obtained were summarized as follows. 1. The sulfametazine residues were liver > kidney > muscle, and among liver, kidney and muscle were very significantly different(p<0.005). 2. The sulfametazine residue in liver was very significantly higher than that in muscle (p<0.005), the sulfametazine residue in kidney was significantly higher than that in muscle (p<0.005), but, the sulfametazine residue in liver was not significantly higher than that in kidney(p<0.05). 3. the sulfametazine residues in swine muscles for export wer exhibited a tendency to lower in small breeding size farms. 4. the sulfametazine fesidue in one of 22 samples of porks for domestic consumption was exceeded 0.1 ppm.

• Environmental Analysis Health and Toxicology
• /
• v.17 no.4
• /
• pp.341-346
• /
• 2002

To investigate the residue of organophosphorus pesticides such as Monocrotophos［Dimethyl-1-methyl-2-methyl carbamoylvinylphosphate］and EPN［O-ethyl-O-4-nitrophenyl phenylphosphonothioate］in the coastal environment of Cheju island, samples of sea organism, water and sediment were collected at the Sackdaldong and Daepodong near the Jungmoon golf course in July and Aug. 1997. The qualified detection limit of mono-crotophos and EPN by GC -FPD were 0.024 ng/g and 0.020 ng/g in the sea organism, respectively Neither Monocrotophos nor EPN was detected in seaweed cava (Ecklonia cava), Agar (Gelidium amansii), turban sell (Batillus cornutus) and sea urchin (Anthocidaris Crassispina). They were not detected in seawater and sediment, either. These results showed that the coastal area near the Jungmoon golf course in the Cheju island was not polluted by the organophosphorus pesticides such as Monocrotophos and EPN.

• BMB Reports
• /
• v.32 no.3
• /
• pp.254-258
• /
• 1999

The NADPH-dependent succinic semialdehyde reductase is one of the key enzymes in the brain GABA shunt, and it catalyzes the formation of the neuromodulator $\gamma$-hydroxybutyrate from succinic semi aldehyde. This enzyme was inactivated by diethylpyrocarbonate (DEP) with the second-order rate constant of $1.1{\times}10^3\;M^{-1}min^{-1}$ at pH 7.0, $25^{\circ}C$, showing a concomitant increase in absorbance at 242 nm due to the formation of N-carbethoxyhistidyl derivatives. Complete inactivation of succinic semialdehyde reductase required the modification of five histidyl residues per molecule of enzyme. However, only one residue was calculated to be essential for enzyme activity by a statistical analysis of the residual enzyme activity. The inactivation of the enzyme by DEP was prevented by preincubation of the enzyme with the coenzyme NADPH but not with the substrate succinic semialdehyde. These results suggest that an essential histidyl residue involved in the catalytic activity is located at or near the coenzyme binding site of the brain succinic semialdehyde reductase.

• Journal of Sericultural and Entomological Science
• /
• v.26 no.1
• /
• pp.21-24
• /
• 1984

The survey and soil analysis were carried to research the problems on mulberry fields applied glutamic acid fermentation residue fertilizer (GAFRF). Mulberry farmers of 364 from 110 si or Gun were chosen at random. The results were as follows; 1. Application rate of three elements applied on mulberry field was 25.8-12.8-16 in non-FAFRF using farmers, whereas 9.8-4.5-5.5kg/10a in GAFRF using farmers. 2. Application rate of organic matter applied on mulberry was 1158kg in non-GAFRF using farmers, whereas 329kg/10a in GAFRF using farmers. 3. Farmers of 53% using FAFRF did not applied three elements, 74% of them organic matter and 50% of them both of three elements and organic matter. These figures increased proportionally with application period of GAFRF. 4. The content of organic matter, availeble P2O5, K, Ca, Mg and pH in soil of appling GAFRF farmers was somewhat lower than in soil of non-appling GAFRF farmers.

• Analytical Science and Technology
• /
• v.7 no.2
• /
• pp.193-199
• /
• 1994

We have synthesized K-CFICs by the modified two-bulb method. These synthesized compound were analysed with X-ray diffractometer and UV/VIS spectrophotometer. According to X-ray diffraction analysis, the intercalation of potassium between the carbon layers of carbon fiber was better then other intercalants. From obtained data for this deintercalation process, we can see that a number of potassiums were presented as a residue between the carbon layers and this residue compounds had a stability for a long term. Therefore, it is appear that these compounds have a capability as new useful material. From UV/VIS spectrometry data, we can suppose that spectrum of K-CFICs synthesized at $250^{\circ}C$ had only a $R_{min}$, and spectra of any other compounds were not appeared charecteristic peaks because potassium ions were little affect the energy levels of original carbon fiber.

• Asian-Australasian Journal of Animal Sciences
• /
• v.13 no.11
• /
• pp.1604-1608
• /
• 2000

Penicillin antibiotics such as penicillin G, ampicillin and amoxicillin have been widely used in the pig industry to control salmonellosis, bacterial pneumonia, and urinary tract infections. Extensive use of antibiotics in veterinary clinics has resulted in tissue residues and bacterial resistance. To prevent unwanted drug residues entering the human food chain, extensive control measures have been established by both government authorities and industries. The demands for reliable, simple, sensitive, rapid and low-cost methods for residue analysis of foods are increasing. In this study, we established a rapid prediction test for the detection of pigs with unacceptable tissue residues of penicillins. The recommended therapeutic doses of three penicillins, penillin G (withdrawal time, 7 days), ampicillin (withdrawal time, 7 days) and amoxicillin (withdrawal time, 14 days), were administered to three groups of 20 pigs each. Blood was sampled before drug administration and during the withdrawal period. The concentration of penicillins in plasma, determined by a semi-quantitative ELISA, were compared to that of internal standard, 4 ppb, which corresponded to the Maximum Residue Limit in milk. The absorbance ratio of internal standard to sample (B/Bs) was employed as an index to determine whether drug residues in pig tissues were negative or positive. That is, a B/Bs ratio less than 1 was considered residue positive, and larger than 1 negative. All 60 plasma samples from pigs were negative to three penicillins at pretreatment. Penicillin G could be detected in the plasma of the treated pigs until day 4 post-treatment and ampicillin until day 2, whereas amoxicillin could be detected until day 10 of its withdrawal period. The present study showed that the semi-quantitative ELISA could be easily adapted to detect residues of penicillin antibiotics (penicillin G, ampicillin and amoxicillin) in live pigs.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
• /
• v.16 no.12 s.103
• /
• pp.1222-1228
• /
• 2005

Analysis on the field uniformity inside of a reverberation chamber was done at 2.45 GHz which is an operating frequency of MWO(Microwave Oven). The Schroeder's Quadratic Residue Diffuser was designed for the chamber, and 3 different types of diffusers have been investigated using the finite-difference time-domain(FDTD) method for the field characteristics of each type. Type 2 and 3 diffusers were filled with dielectric material, and the size of these could be designed smaller than Type 1, without degradation of field characteristic. Type 3 diffuser shows better results among the three types of diffusers in view of increased test region and better field uniformity.

• Asian-Australasian Journal of Animal Sciences
• /
• v.13 no.12
• /
• pp.1775-1778
• /
• 2000

Oxytetracycline has been widely used in the cattle industry to control pneumonia, shipping fever, foot rot, bacterial enteritis, and uterine infections. Extensive use of antibiotics in veterinary clinics has resulted in residues in tissue and bacterial resistance to antibiotics. To prevent unwanted drug residues from entering the human food chain, extensive control measures have been established by both government authorities and industries. The demands for reliable, simple, sensitive, rapid and low-cost methods for residue analysis of foods are increasing. In this study, we established a rapid test for tissue residues of oxytetracycline in cattle. The recommended therapeutic dose of oxytetracycline (withdrawal time, 14 days) was administered to 10 cattle. Blood samples were collected from each cow before drug administration and during the withdrawal period. The concentration of oxytetracycline in plasma, determined by a semi-quantitative ELISA, was compared to that of the internal standard, 10 ppb. The absorbance ratio of internal standard to sample (B/Bs) was employed as an index to determine whether the residues in cattle tissues were negative or positive. That is, a B/Bs ratio less than 1 was considered as residue positive and that greater than 1 as negative. Based on this criterion, all plasma samples from cattle were negative to oxytetracycline at pre-treatment. Oxytetracycline could be detected in the plasma treated cattle until day 14 post-treatment. The present study showed that the semi-quantitative ELISA could be easily adapted in predicting tissue residues for oxytetracycline in live cattle.

• Microbiology and Biotechnology Letters
• /
• v.33 no.3
• /
• pp.184-188
• /
• 2005

IgE-dependent histamine-releasing factor (HRF) is found extracellularly to regulate the degranulation process of histamine in mast cells and basophils and known to play a predominant role in the pathogenesis of chronic allergic disease. HRF has been also identified in the intracellular region of the cell. Previously, we reported that HRF interacts with the 3rd cytoplasmic domain of the alpha subunit of Na,K ATPase and inhibits Na,K-ATPase activity. The predicated phosphorylation site in HRF by PKC was mapped to one serine residues (S98) by the computer analysis. In this study, we identified that S98 residue of HRF was phosphorylated using anti-HRFpS98 antibody which specifically recognizes the phosphorylated serine residue of HRF and HRFS98A mutant construct. We also performed $^{86}Rb^{+}-uptake$ assay to understand the role of HRF wild-type and HRFS98A mutants on the regulation of Na,K-ATPase activity. Dephosphorylation of HRF at serine 98 residue recovers slightly the inhibitory function of HRF, suggesting that phosphorylated HRF at serine 98 may not suppress the Na,K-hfpase activity.

• Journal of the Korean Applied Science and Technology
• /
• v.33 no.4
• /
• pp.658-666
• /
• 2016

We produced the Red ginseng residue water(RGW), ethanol(RGE), 1,3-butylene glycol(RGB) extract from Red ginseng residues, analyzed the components of the extracts by HPLC, and evaluated the cell viability on B16F10, antioxidant and anti-wrinkle effects for application of cosmetics. As a result, RGW, RGE, RGB have various ginsenoside and its content of RGB were higher than RGW, RGE as component analysis by using high performance liquid chromatography(HPLC). RGW showed similar with RGB in cell viability on B16F10 which were higher than RGE. DPPH radical scavenging activity increased according to the RGE>RGB>RGW. SOD-like activity increased according to the RGB>RGE>RGW. Also, elastase inhibition effect increased according to the RGW>RGB>RGE. These results suggested that RGB and RGW may have potential for the application of antioxidant and anti-wrinkle effects for cosmetics.