• Journal of Food Hygiene and Safety
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• v.9 no.1
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• pp.1-13
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• 1994

A Simultaneous determination method was improved for the determination and confirmation of zeranol, zearalenone, as well as their isomers and metabolites, in beef. The analytes were extracted from tissue by CH3CN, hydrolyzed enzymatically(for glucuronide conjugates), cleaned up by a strong basic anion exchange resin combined with a liquid/liquid partitioning, derivatized using MSTFA and confirmed, quantified by GC/MS/SIM with a internal standard, zearalane. The results were as follows : (1) all the estrogens were separated on the GC/MS chromatogram under the extraction method and the chromatographic conditions improved, the retention times of zearalane-TMS2, zearalanone-TMS2, zearalenone-TMS2, zeranol-TMS3, taleranol-TMS3, and $\alpha$-zearalenol-TMS3, $\beta$-zearalenol-TMS3, were 18.49, 19.44, 19.63, 19.71, 19.79 and 19.99, 20.08 minutes, respectively. (2) The calibration curves of residual zeranol, zearalenone and their metabolites showed constantly linear(r=0.99) in the range of 5~20 ng. The minimum detection concentration of residual zeranol, zearalenone and their metabolites was 1 ppb. (3) The total average recovery of residual zeranol, zearalenone and their metabolites from spiked beef was 60.2%(CV=29.7%) at the 1 ppb and 63.5%(CV=26.5) at the 2 ppb, 72.9%(CV=18.2%) at the 4 ppb. (4) The preservation method for 6 estrogens was improved for the fast running time(21 min) and MSTFA was utilized for derivatizing 6 estrogens for improvement of recovery, for good resolution, for characteristic mass spectra unlike Jose's method and Tina's method. The utilization of zearalane as internal standard showed good quantification result for zeranol, zearalenone, as well as their isomers and metabolites, in beef.

• Journal of Food Hygiene and Safety
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• v.8 no.1
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• pp.9-15
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• 1993

In an attempt to quantitate and qualitate residual antibiotics and antibacterial agents in meat simultaneously, we studied a gas chromatography-mass spectrometry(GC/M8) analysis. For a simultaneous analysis of penicillin G, chloramphenicol and thiamphenicol in meat, a simple and rapid clean-up procedure including extraction with 0.01 M EDTA-2Na Mcilvaine buffer (pH 4.0), defatting with n-hexane, and elution with 0.01M-methanolic oxalic acid from Bond Elute $C_{18}$ cartridge, and quantitation by selected ion monitoring (SIM) mode after derivatization was performed. The recoveries (%) of penicillin G, chloramphenicol and thiamphenicol (CV, %) at 1 ppm fortification level were 63.5 (7.6), 76.3 (8.1) and 84.7 (2.0), and the detection limits of those were 0.6, 0.085 and $0.084\;\mu\textrm{g}$ beef, respectively. This method using 81M mode allows excellent detection and quantitation of residual antibiotics and antibacterial agents in meat. Moreover, confirmation by a full scan electron impact mass spectrum is possible if residual level in the sample in above 1 ppm.

• Analytical Science and Technology
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• v.10 no.3
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• pp.168-178
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• 1997

The optimum conditions for the residual analysis of the ethalfluralin herbicide on soils were investigated and the residues in soils were determined by gas chromatography with electron capture detector(GC-ECD). The soil samples extracted with methanol and dichloromethane and concentrated. The concentrated phase were redissolved with toluene and analyzed with GC-ECD after separated by cyanosilica gel Sep-Pak cartridge. From the standard addition experiments with 0.1 and 1.0ppm, the average recoveries were 92.8~101.2% and the detection limit was 0.004ppm. The half-life time of ethalfluralin in the soil(A) was 35 days in the laboratory and 7.2 days in the field test whereas it was 45 days and 9.7 days for each in case of soil(B).

• Analytical Science and Technology
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• v.6 no.3
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• pp.283-288
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• 1993

The solvent extraction and clean up processes for the gas chromatographic determination of muarimol pesticide residues in apples were investigated and examined the changes of residual concentration with the passage of time. The extracted pesticide with methanol were partitioned with dichloromethane after adding sodium chloride solution. The separated solutions were concentrated and transfered to the alumina column for clean up, and eluated with 1-chlorobutane : methanol solution. As a results their recovering for 0.200 and 1.00ppm muarimol spiked on apples have shown 79~95%. Residual amounts of nuarimol in apple was 0.0830ppm when the fungicide was treated eight times until 3 days before its harvest. It seems to be safely used when nuarimol is treated six times until 7 days before harvest of apple.

• Conservation Science in Museum
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• v.2
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• pp.51-55
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• 2000

This study is an analysis of residual fatty acid of the unidentified 26 fish bone samples which were excavated from Bupchon-ri in Wonju, Kangwon province. The purpose of this study is to identify fish kind by comparing the residual fatty acid analysis with the reference data of croaker, shark, herring and weakfish. Fatty acid was separated by gas chromatography and distribution pattern was analyzed by calculating composition of each sample. Principal component analysis(PCA), one of multivariate analysis method was used to understand fatty acid distribution data.

• Journal of Korea Foundry Society
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• v.42 no.1
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• pp.61-66
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• 2022

Hydraulic units are important components of agricultural and construction machinery, and thus require high-quality castings. However, gas defects occurring inside the sand cores of the castings due to the resin used is a problem. This study therefore aimed to develop a casting simulation method that can clarify the gas defect positions. Gas defects are thought to be caused by gas generated after the molten metal fills up the mold cavity. The gas constant is the most effective factor for simulating this gas generated from sand cores. It is calculated by gas generating temperature and analysis of composition in the inert gas atmosphere modified according to the mold filling conditions of molten metal. It is assumed that gases generated from the inside of castings remain if the following formula is established. [Time of occurrence of gas generation] + [Time of occurrence of gas floating] > [Time of occurrence of casting surface solidification] The possibility of gas defects is evaluated by the time of occurrence of gas generation and gas floating calculated using the gas constant. The residual position of generated gases is decided by the closed loops indicating the final solidification location in the casting simulation. The above procedure enables us to suggest suitable casting designs with zero gas defects, without the need to repeat casting tests.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.5 no.3
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• pp.31-39
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• 1996

The objective of this study is to investigate the influence of welding residual stresses on the fatigue crack growth behavior of cracks located transverse to the weld bead. For this purpose, G. T. A (Gas Tungsten Arc) welding was performed on hte Al alloy 1100-O plate and the same initial crack is made on HAZ(Heat Affected Zone), weld metal and base meta respectively. Specimens were used CT(Compact Tension) specimens. Initial welding residual stresses were measured by using strai gage sectioning method. All specimens were tested under constant amplitude load with stress ratio R=0.1, It is possible to predict fatigue crack growth behaviors and the fatigue life, using numerical analysis together with distribution of initial residual stress and the values of C and m obtained from $da/dN-{\Delta}K$

• Proceedings of the Korean Society of Machine Tool Engineers Conference
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• 1996.03a
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• pp.177-182
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• 1996

The objective of this thesis is to investigate the influence of welding residual stresses on the fatigue growth behavior of welding residual stresses on the fatigue growth behavior of cracks located transverse to the weld bead. For this purpose, G.T.A. (Gas Tungsten Arc) welding was performed on the Al. Alloy 1100-0 plate and the same initial crack is made on HAZ(Heat Affected Zone), weld metal and base metal respectively. C.T.(Compact Tension) specimens were used as experimental material. Initial welding residual stresses were measured by using strain gage sectioning method. All specimens were tested under constant amplitude load with stress ratio R=0.1

• Proceedings of the KWS Conference
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• 2002.10a
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• pp.390-395
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• 2002

The experimental and numerical study has been conducted on the sleeve repair welding of API 5L X65 pipeline. SMA W and GTAW were applied to weld the sleeve. The macrostructure and hardness of repair welds have been examined. The [mite element analysis of the multi-pass sleeve-fillet welding has been conducted to validate the experiment and investigate the effects of in-service welding conditions. The effect of gas flow rate on the hydrogen cracking was investigated. The effect of internal pressure on residual stresses and plastic strain was investigated. The allowable heat input was predicted considering the maximum temperature of inner surface of pipe and cooling rate at CGHAZ.

• International Journal of Korean Welding Society
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• v.2 no.2
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• pp.36-41
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• 2002

The experimental and numerical study has been conducted on the sleeve repair welding of API 5L X65 pipeline. SMAW and GTAW were applied to weld the sleeve. The macrostructure and hardness of repair welds have been examined. The finite element analysis of the multi-pass sleeve-fillet welding has been conducted to validate the experiment and investigate the effects of in-service welding conditions. The effect of gas flow rate on the hydrogen cracking was investigated. The effect of internal pressure on residual stresses and plastic strain was investigated. The allowable heat input was predicted considering the maximum temperature of inner surface of pipe and cooling rate at CGHAZ.