• Journal of fish pathology
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• v.6 no.2
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• pp.205-218
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• 1993

On the development of hirame(Paratichtys olivaceus) culture, outbreak of scuticociliata infection was reported to cause severe damage in Japan. To establish effective measures for isolation and cultivation of this ciliate, we tried to culture this pathogenic ciliate using medium for bacteria and fish cell lines in vitro. Scuticociliata from the brain tissues of infected fish was aseptically inoculated to CHSE-214 cells cultured in MEM-10 without antibiotic. Scuticociliata grew well and the number of ciliate reached $10^6\;cells/ml$ at temperatures of $15^{\circ}C$ to $20^{\circ}C$ for 10d. The number of ciliate cultured in the cell lines is 10 times higher than the numbers cultured in the liquid medium alone. This ciliata could be cloned by dilution method. Scuticociliata isolated could grow well on 42 different cell lines that were established from marine fish, warm freshwater fish, and salmonids. This ciliate could be preserved in liquid nitrogen for more than 6 months. Subsequently, we observed the optimal temperature and salinity for growth, and tested the sensitivities of this organism to formaldehyde, flagyl(Metronidazole), Ekuteshin(Combination compound of sulfamonometoxin and ormethoprim), and ozonixation. Optimal temperature for growth was $25^{\circ}C$ and salinity was 1.0 to 1.5%. Washed scuticociliata was killed by formaldehyde at the concentration of 50ppm for 10min, but was not completely killed even at a high concentration of 400ppm for 20min in MEM-5. Flagyl and Ekuteshin can inhibit the growth of scuticociliata at the concentration of 1,000 and $100{\mu}g/ml$ in MEM-10, respectively. More than 99% of this scuticociliata could be killed by ozonization at a dose equivalent to $1.0mg/\ell$ oxidant for 30sec in sea water. Isolated scuticociliata showed the pathogenicity to the cultured hirame by artificial infection(I. P. injection, $10^5\;cells$/fish). The number of scuticociliata in the water could be counted by most probable number(MPN) method using tissue culture, and the minimum detectable number was $1.8\;cells/\ell$. The number in the reservoir tank for water supply to the culture tank was 110 cells/l. After cleaning by elimination of the sediments from of the reservoir tank and disinfected with formaldehyde, number of scuticociliata decreased and was counted less than $1.8\;cells/\ell$ and infection rate of cultured hirame was decreased.

• Journal of Environmental Health Sciences
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• v.38 no.6
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• pp.503-509
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• 2012

Objectives: This study was performed in order to evaluate the generation characteristics of airborne bacteria and fungi while operating a household humidifier, in consideration of user habits. Methods: Microbial samples were collected in a closed chamber with a total volume of 2.76 $m^3$, in which a humidifier was operated according to experimental strategies. A cultivation method based on the viable counts of mesophilic heterotrophic bacteria and fungi was performed. Experimental strategies were divided into three classes: the type of water in the water reservoir (tap water, cooled boiled water); the frequency of filling the reservoir (refill every day, no refill); and the sterilization method (sterilization function mode, humidifier disinfectants). Results: Significant increases in the concentration of airborne bacteria were observed while the humidifier was in operation. The concentration had increased to 2,407 $CFU/m^3$ by 120 hours when tap water filled the reservoir without any application of sterilization, while for cooled boiled water, it was merely 393 $CFU/m^3$ at a similar time point. Usages of disinfectant in the water tank were more effective in decreasing bioaerosol generation compared to sterilization function mode operation. Generation characteristics of airborne fungi were similar to those of bacteria, but the levels were not significant in all experiments. Calculated exposure factor can be used as an indicator to compare biorisk exposure. Conclusion: This study identified the potential for bioaerosol generation in indoor environments while operating a household humidifier. User practices were critical in the generation of bioaerosol, or more specifically, airborne bacteria. Proper usage of a humidifier ensures that any biorisks resulting from generated bioaerosol can be prevented.

• Journal of Korea Water Resources Association
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• v.57 no.1
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• pp.59-72
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• 2024

Agricultural reservoirs supply water for various purposes such as irrigation, maintenance, and living. Since agricultural reservoirs respond sensitively to seasonal and climate changes, it is essential to estimate supply and inflow for efficient operation, and water management should be done based on these data. However, in the case of agricultural reservoirs, the measurement of supply and inflow is relatively insufficient compared to multi-purpose dams, and inflow-supply analysis in agricultural reservoirs through water balance analysis is necessary for efficient water management. Therefore, rainfall-runoff analysis models such as ONE model and Tank model have been developed and used for reservoir water balance analysis, but the applicability analysis for ungauged watersheds is insufficient. The ONE model is designed for daily runoff calculation, and the model has one parameter, which is advantageous for calibration and ungauged watershed analysis. In this study, the water balance was analyzed through the ONE model and the Tank model for 15 watersheds upstream of dams, and R² and NSE were used to quantitatively compare the performance of the two models. The simulation results show that the ONE model is suitable for predicting the inflow of agricultural reservoirs with the ungauged watershed

• Journal of Korea Water Resources Association
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• v.51 no.12
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• pp.1171-1180
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• 2018

Korea's water transmission system is operated by the nonpressure flow method that flows from highlands to lowlands due to the nature of Korea with many mountainous areas. In order to store water in the highlands, the water pumps are installed and operated. However, In this process, a lot of electrical energy is consumed. therefore, it is necessary to minimize the energy consumption by optimizing the size and operation schedule of the water pumps. The optimal capacity and operation method of the water pump are affected by the size of the tank (distributing reservoir). Therefore, in order to economically design and operate the water transmission system, it is reasonable to consider both the construction cost of the water pump and the tank and the long-term operation cost of the water pump at the step of determining the scale of the initial facilities. In this study, the optimum design model was developed that can optimize both the optimal size of the water pump and the tank and the operation scheduling of the water pump by using the genetic algorithm (GA). The developed model was verified by applying it to the water transmission systems operated in Korea. It is expected that this study will help to estimate the optimal size of the water pump and the tank in the initial design of the water transmission system.

• Proceedings of the Korea Air Pollution Research Association Conference
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• 2003.05b
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• pp.91-92
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• 2003

경제성장 및 산업의 고도화에 따른 자동차 보급의 증가, 유류 및 유기용제의 사용량 증가로 휘발성유기화합물질(Volatile Organic Compounds; 이하 VOCs)의 배출량이 증가하고 있다. 이러한 VOCs의 배출원중에 하나가 주유소에서 발생하는 VOCs이다(정일록 등, 1995). 주유소에서의 VOCs 배출원은 EPAAP-42의 경우 지하저장탱크 숨구멍을 통한 증발과 유조차에서 지하 저장탱크에 휘발유 등 석유 제품을 하역시 증발을 Stage I, 지하저장에서 자동차에 주유시 증발 및 주유시 흘림에 의한 VOCs 증발을 Stage II로 구분되어 있다. (중략)

• Magazine of the Korean Society of Agricultural Engineers
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• v.37 no.1
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• pp.65-72
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• 1995

This paper describes the applications of the SCS TR-20 hydrologic model for simula- tion of hourly inflow rates from sixty-six ungaged tributaries and subareas between the Naju station and the estuarin dam at the Yongsan River Basin. The model was tested for the ungaged conditions with fifteen storm events at Naju station. Hourly simulated run- off data were compared with the observed, and the results showed less correlationships between the two data than those from TANK model. The coefficients of correlation ranged from 0.74 to 0.87. The curve numbers and time of concentration were defined from topographic dta for each of sixty-six tributaries for the estuarine dam and used for TR-20 applications. The results were within an acceptable range of errors in simulating the inflow fluctuations for the flood forecasting at the estuarine dam.

• Journal of the Korean Society of Safety
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• v.20 no.3 s.71
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• pp.42-46
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• 2005

The static electricity by thinner flow and discharge energy is investigated experimentally for the purpose of preventing the electrostatic discharge and damage. Test system for evaluating streaming electrification consists of a teflon pipe, a reservoir tank a pump, flowmeters and an electrometer. When dielectric liquid flows through a pipe from one vessel to another, the potential difference generated in the collecting vessel is due to the accumulation of charges. These charges result from the convection of a part of the electrical double layer existing in the tube at the contact between the liquid and the inner wall. When the fluid velocity increases, the electric current increases proportionally. The charging current and accumulated charges by streaming electrification at the thinner velocity of 40cm/s are measured a range of 5 nA and $0.27{\mu}C$ respectively. This amount of static discharge energy generated by streaming electrification is enough to ignite flammable solvent. Therefore surface electric potential should decrease by using electrostatic shielding and ground.

• Structural Engineering and Mechanics
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• v.20 no.4
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• pp.389-403
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• 2005

In the present paper it is aimed to perform the stochastic dynamic analysis of fluid and fluidstructure systems by using the Lagrangian approach. For that reason, variable-number-nodes twodimensional isoparametric fluid finite elements are programmed in Fortran language by the authors and incorporated into a general-purpose computer program for stochastic dynamic analysis of structure systems, STOCAL. Formulation of the fluid elements includes the effects of compressible wave propagation and surface sloshing motion. For numerical example a rigid fluid tank and a dam-reservoir interaction system are selected and modeled by finite element method. Results obtained from the modal analysis are compared with the results of the analytical and numerical solutions. The Pacoima Dam record S16E component recorded during the San Fernando Earthquake in 1971 is used as a ground motion. The mean of maximum values of displacements and hydrodynamic pressures are compared with the deterministic analysis results.

• Journal of Korean Society of Disaster and Security
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• v.12 no.4
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• pp.1-13
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• 2019

Recently, as the occurrence frequency of sudden floods due to climate change increased, it is necessary to install the facilities that can cope with the initial stormwater. Most researches have been conducted on the design of facilities applying the Low Impact Development (LID) and the reduction effect on rainfall runoff to examine with 1D or 2D numerical models. However, the studies on the examination about flow characteristics and stability of pipe network systems were relatively insufficient in the literature. In this study, the stability of the pipe network system in rainwater storage tank was examined by using 3D numerical model, FLOW-3D. The changes of velocity and dynamic pressure were examined according to the number of rainwater storage tank and compared with the design criteria to derive the optimal design plan for a rainwater storage tank. As a results of numerical simulation with the design values in the previous study, it was confirmed that the velocity became increased as the number of rainwater storage tank increased. And magnitude of the velocity in pipes was formed within the design criteria. However, the velocity in the additional rainwater storage pipe was about 3.44 m/s exceeding the allowable range of the design criteria, when three or more additional rainwater storage tanks were installed. In the case of turbulence intensity and bottom shear stress, the bottom shear stress was larger than the critical shear stress as the additional rainwater storage was increased. So, the deposition of sediment was unlikely to occur, but it should be considered that the floc was formed by the reduction of the turbulence intensity. In addition, the dynamic pressure was also satisfied with the design criteria when the results were compared with the allowable internal pressure of the pipes generally used in the design of rainwater storage tank. Based on these results, it was suitable to install up to two additional rainwater storage tanks because the drainage becomes well when increasing of the number of storage tank and the velocity in the pipe becomes faster to be vulnerable to damage the pipe. However, this study has a assumption about the specifications of the rainwater storage tanks and the inflow of stormwater and has a limitation such that deriving the suitable rainwater storage tank design by simply adding the storage tank. Therefore, the various storage tank types and stormwater inflow scenarios will be asked to derive more efficient design plans in the future.

• Korean Journal of Soil Science and Fertilizer
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• v.41 no.6
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• pp.374-378
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• 2008

For the standard method of collecting the run-off, it is consumed the high cost and much effort to install and to manage this instrument. Because the all the soil and water from reservoir tank must be eliminate after their measurement of amount of soil loss and run-off and installed the reservoir tank at regular size in the experimental field. Therefore, objective of this study was to compare its efficacy between the standard method and a flow rotate divider for ontinuously collecting and measuring the soil loss and run-off in order to conveniently conduct the field experiment of the lysimeters. For collecting the sampling of soil loss and run-off from agricultural land with invariable ratio, a flow rotate divider was consisted with a 8 blades of round plate sloped in order to collect the invariable ratio of soil and water at lowest part from round plate by the law of gravity. For comparing its accuracy in the batch scale experiment, it shown that there was significantly a positive linear corelation ($r=0.997^{***}$) between flowing and sampling amounts with adjusting the range from 1 to $10L\;min^{-1}$ with flowing rate. In collecting ratio in the field experiment, it observed that the more its accuracy had, the more soil loss and run-off.