• Title/Summary/Keyword: Research and Development Department

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Phylogenetic relationships of Lyophyllum decastes on the based of ITS region sequences and RAPD (잿빛만가닥버섯(Lyophyllum decastes)의 ITS 영역염기서열 및 RAPD에 의한 계통학적 유연관계 분석)

  • Woo, Sung-Mi;Park, Yong-Hwan;Yoo, Young-Bok;Shin, Pyung-Gyun;Jang, Kab Yeul;Jin, Yong-Ju;Sung, Jae-Mo
    • Journal of Mushroom
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    • v.7 no.3
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    • pp.98-104
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    • 2009
  • Phylogenetic relationships of Hypsizygus mamoreus and Lyophyllum decastes artificial cultivated using ITS sequences and RAPD polymorphism have been analyzed. Based on ITS region sequences of 14 strains, we could divide into 2 group as group1 to Hypsizygus mamoreus and the control isolated group2 to Lyophyllum decastes were identified as the same species. Restrict analysis of rDNA ITS region which was amplified by PCR, 14 collected strains could be classified into 4 clusters. There was approximately 58% genetic similarity between cluster I(SPA 100, 101, 102) and cluster II(SPA 200, 208 and SPA 201, 202), 41% between cluster III(SPA 104, 103, 203) and cluster IV(SPA 204, 206, 207, 205) by BLAST analysis. RAPD polymorphisms were used to analyze the species diversity of artificially cultivated Lyophyllum decastes SPA 202. As a result, similarity between SPA 202 and SPA 203 was 75%, at the same time, similarity between SPA 202 and Pleurotus eryngii SPA 103 and SPA 104 was 65%.

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Consumer's Perception, Preference and Intake Frequency of Jangachi(Korean Pickle) by Age for Developing Low Salt Jangachi (저염 장아찌 개발을 위한 연령별 소비자 인식, 기호도 및 섭취빈도 조사)

  • Weon, Mi-Keyoung;Lee, Yeon-Jung
    • Culinary science and hospitality research
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    • v.19 no.5
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    • pp.249-263
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    • 2013
  • This study was performed to analyze the perception, preference and intake frequency of Jangachi(Korean Pickle) in order to develop low salt healthy Jangachi(Korean pickle). The findings are summarized as follows: The reason for eating Jangachi was 'Stimulation of appetite(47.8%).' The problem of commercial Jangachi was 'having high Na and sodium contents(59.1%).' The most influential improvement points for development of low-sodium Jangachi was 'Sanitarily distributed,' followed by 'not too salty,' 'low price,' and 'safe to store.' The important items of manufacture factor for making low-sodium Jangachi were safety(4.36 points), sanity, safekeeping and storage, and quality of ingredients. The important items of quality factor were taste(4.30 points), salinity, nutrition and temperature. The most preferred and frequently intake Jangachi was 'garlic Jangachi', followed by 'perillar leaf Jangachi', 'dried radish Jangachi', 'onion Jangachi', 'pepper Jangachi', 'garlic stem Jangachi', 'cucumber Jangachi', 'radish Jangachi', and 'soy leaf Jangachi'.

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Oxidative Stress and Antioxidant Responses in Poplar Clones Irrigated with Livestock Waste Leachate (양돈폐수 처리에 의한 포플러클론의 산화스트레스와 항산화반응)

  • Je, Sun-Mi;Yeo, Jin-Gi;Woo, Su-Young
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.13 no.3
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    • pp.140-147
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    • 2011
  • We studied the influence of livestock waste leachate on oxidative damage and antioxidative responses in poplar clones in August which increase the demand of antioxidants because of high temperature and high light during this period. We measured ion leakage, antioxidant enzyme activities (APX, GR), and carotenoid contents. Oxidative damage and antioxidative responses by treated livestock waste leachate in poplar clones showed various results. We divided poplar clones into three groups using the criteria based on ion leakage which represent cell damage induced oxidative stress. Eco 28, 62-10, Bonghwa1 and Dorskamp belonged to the first group in which the cell damaged level was lower than that of the control. The results suggest that this group augmented for demand of antioxidative in summer because high concentration of nitrogen induced by treatment of live stock wastes acted as environmental stress. Consequently, they failed to keep up the homeostasis of reactive oxygen species. The second group in which the cell damaged level was similar to that of the control was Suwon, 72-30 and 72-31 clones. Finally, 97-18 clone belonged to the third group in which the cell damaged level was lower than that of the control group. In this case, nitrogen treated by livestock waste leakage decreased oxidative stress. 97-18 clone was the clones with the least damage in summer oxidative stresses treated by livestock waste leakage. These results suggest that the high concentration nitrogen due to the livestock waste leakage can act differently upon the clones. We speculate that the added oxidation damage in the summer (growing season) may have an effect on the total fresh weight and also influence the purification ability for livestock waste leakage. However, further studies are needed for the confirmation.

Evaluation on national environmental functionality of farming on soil loss using the USLE and replacement cost method (USLE모형과 대체법을 이용한 밭농사의 토양유실 저감기능 계량화 평가)

  • Hyun, Byung-Keun;Kim, Moo-Sung;Eom, Ki-Cheol;Kang, Ki-Kyung;Yun, Hong-Bae;Seo, Myung-Cheol;Sung, Ki-Seog
    • Korean Journal of Soil Science and Fertilizer
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    • v.35 no.6
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    • pp.361-371
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    • 2002
  • Multifunctionality of agriculture has been an important international issue in terms of environmental benefits and public concerns. We calculated soil loss mass in national basis using the USLE, and attempted to evaluate its economical benefits by replacement cost method. Soil loss mass ranged from 1.4 to $18MT\;ha^{-1}\;yr^{-1}$ was fairly fitted to measured values for 13 cropping systems. In national basis, the factors in USLE were evaluated as: 429.4 for rainfall and runoff factor. R, 0.15 for soil erodibility factor, K, 1.72 for topographic factor, LS, 0.275 for cover and management factor, C, and 0.856 for support practice factor, P. The soil loss estimated from upland farming using the USLE was $26.1MT\;ha^{-1}\;yr^{-1}$, but soil loss from the bare soil was $110.8MT\;ha^{-1}\;yr^{-1}$, the ratio of soil loss from upland farming to bare soil was 23 percents. Function of reducing soil loss in comparison with the bare soil was $84.7MT\;ha^{-1}\;yr^{-1}$, of which national soil loss mass was 62.6 million MT per annum in south Korea. Agriculture economic replacement cost of soil loss reduction was 497 billion Wons(398 million dollars) for the cost of upland soil dressing. For conservational purposes to increase the environmental benefits of upland farming, the agricultural practice including contour, strip cropping, terracing and division ditches should be implemented.

Development of Coupler for Live Cable Fault Detection Based on Reflectometry (반사파 계측법 기반의 활선 케이블 고장 검출을 위한 커플러의 개발)

  • Jeon, Jeong-Chay;Oh, Hun
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.9
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    • pp.401-406
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    • 2016
  • When measuring live cable faults and their location based on reflectometry, a coupler is placed between the cable and the test system. This coupler prevents damage to the test circuits by indirectly measuring the live voltage of the cable using reflectometry. It also provides a coupling path that allows the transmission and receive signal to pass into the cable. In this study, we design and construct a contact coupler to locate faults in both dead and live cables using reflectometry. The proposed coupler is of the inductive coupling type and is constructed after the calculation of the signal transmission loss by simulation. The performance of the developed coupler is tested by measuring the transmission loss and frequency flatness. The results showed that the transmission signal loss is less than -1.98dB in the frequency bandwidth above 1 Mhz. The reflectometry system was designed based on sequence time domain reflectometry (STDR) and spread spectrum time domain reflectometry (SSTDR) in order to apply it to the detection of faults and their location in live cables and tests on live cables were performed. The test results showed that the proposed coupler can be used in a reflectometry system for live cable fault detection.

Evaluation of Lateral Strength and Ductility of Velcro Reinforced RC Columns with Finite Element Analysis (유한요소해석을 통한 벨크로로 보강된 RC 기둥의 횡방향 강도 및 연성 능력 평가)

  • Kim, Sang-Woo;Kim, Kyeong-Min;Kim, Geon-Woo;Lee, Su-Young;Kim, Jin-Sup
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.25 no.4
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    • pp.12-19
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    • 2021
  • Recently, with frequent earthquakes around the world, research on seismic design and seismic reinforcement of reinforced concrete facilities has been actively conducted from earthquakes. In particular, columns, which are compressed members of reinforced concrete structures, are vulnerable to lateral forces caused by earthquakes, so an appropriate seismic reinforcement method is required. Therefore, this study intended to develop Velcro seismic reinforcement method that is quick and easy to construct. For the development of Velcro seismic reinforcement, the adhesion and tensile strength of the existing industrial velcro was improved. A direct tensile test was also conducted to compare the tensile performance of the newly-developed velcro seismic reinforcement to industrial one. In addition, numerical analysis was performed to predict the seismic performance of RC columns reinforced by industrial and newly-developed velcro. Based on the analysis results, the strength and ductility of the non-seismic and velcro-reinforced RC column were reviewed. The analysis confirmed that both the strength and ductility of non-seismic RC columns reinforced by industrial and newly-developed velcro increased, but the seismic performance of the newly-developed Velcro reinforcement is better than that of industrial velcro.

A study on the life of immigrant youth before and after entering Korean society: Focusing on Korean-Chinese adolescents (중도입국청소년의 한국사회 입국 전·후 삶에 대한 연구 -조선족 청소년을 중심으로-)

  • Song, MinKyoung;Kim, JinWon
    • Journal of the Korean Society of Child Welfare
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    • no.58
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    • pp.103-139
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    • 2017
  • The objective of this study was to examine in depth the life of Korean-Chinese adolescents before and after entering Korean society. These adolescents experienced early separation from their biological mothers in China and were raised by relatives due to the absence of parents. The adolescents' childhoods were filled with a sense of loneliness and emptiness, which made them feel hopeless in life, and traveling to Korea in hope and fear was a major challenge in their lives. After coming to Korea, they harmed themselves by engaging in toxic behavior and found themselves hopeless. They also experienced difficulties due to the relationships that needed to be rebuilt with their biological parents, as well as the relationships with their stepparents and half-siblings. However, they reported feeling self-confident and hopeful that their life would improve in the future. In addition, family support and social-support systems have given them a potential avenue for development. Verbal data collected from seven Korean-Chinese adolescents were analyzed. Based on the results, practical recommendations to be reflected in related programs were provided.

A Study on the Operation Plan of Powered Wheelchair and Electric Scooter Charging Station (전동휠체어 및 전동스쿠터 충전소 운영 방안 연구)

  • Kim, Seung Eon;Kim, Kyung Sik;Kang, Jung Bae;Song, Byung Seop
    • 재활복지
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    • v.21 no.2
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    • pp.191-216
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    • 2017
  • Recently, according to demands for a mobility right of people with disabilities, the use of powered mobility device has been rapidly increased and recharging facilities have been set up in many places of country. But the standards for the installation and management of the facilities are not clear and many problems are brought up in operating the facilities. Therefore, regulations and realities of recharging facilities were investigated and the user survey was progressed. On the basis of the research results, a rational and efficient installation and management plan was drawn up. The plan suggests that the recharging facilities should be installed in a building managed by government and many kinds of public building like library, theater, sports facilities, department store, museum, etc. Further, the recharging facilities should secure independent place which have specified area for protecting of privacy and provide some amenities for people with disabilities to enhance the conveniences.

Biological Roles of the Glycan in the Investigation of the Novel Disease Diagnosis and Treatment Methods (신개념 질병 진단 및 치료 연구에 있어서의 당사슬의 생물학적 역할)

  • Kim, Dong-Chan
    • Journal of Life Science
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    • v.28 no.11
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    • pp.1379-1385
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    • 2018
  • Glycans are attached to proteins as in glycoproteins and proteoglycans. They are found on the exterior surface of cells. O- and N-linked glycans are very common in eukaryotic cells but may also be found in prokaryotes. The interaction of cell surface glycans with complementary glycan binding proteins located on neighboring cells, other cell types, pathogens like virus, or bacteria is crucial in biologically and biomedically important processes like pathogen recognition, cell migration, cell-cell adhesion, development, and infection. Their implication in pathological condition, suggests an important role for glycans as disease markers. In addition, a great amount of research has been shown that appropriate glycosylation of a recombinant therapeutic protein is critical for product solubility, stability, pharmacokinetics and pharmacodynamics, bioactivity, and safety. Besides, cancer-associated glycosylation changes often involve sialic acid in glycan branch which play important roles in cell-cell interaction, recognition and immunological response. This review aims at giving a comprehensive overview of the glycan's biological function and describing the relevance among the glycosylation, disease diagnosis and treatment methods. Furthermore, the high-throughput analytic methods available to measure the profile changing patterns of glycan in the blood serum as well as possible underlying biochemical mechanisms.

The Development of an Astronomical Observing Education Program for High School Science Club Activities - Inquiring Distances of Open Clusters Using Small Telescopes - (고등학교 과학동아리 천체 관측 교육 프로그램 개발 - 소형 망원경을 활용한 산개성단의 거리 탐구 -)

  • Choi, Dong-Yeol;Yoon, Ma-Byong
    • Journal of the Korean earth science society
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    • v.40 no.3
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    • pp.300-312
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    • 2019
  • The purpose of this study is to develop an astronomical observing education program that enables high school students to inquire the distance of astronomical bodies based on the research methods (observing open clusters and exploring collected big data) using small telescopes and DSLR cameras. After analyzing the 2015 revised science curriculum, we developed science club activity materials and teacher-student learning contents suitable for high school earth science education. A panel of six teachers and researchers of earth science education and astronomy, participated in developing the educational materials. The validity of the program was verified through establishing the agreement among the panels after in-depth discussions and clarifications. The program, developed with 10 lessons in total, showed high satisfactory content validity (CVI, .89) and conformity of school class (Likert's 5 point scales, 4.17). The feedback of the panels and the Delphi analysis continued to improve the quality of the program. The pilot testing result with high school students (N=9) showed that the students' satisfaction rate was high as 4.48. Using the astronomical observational education program of this study is expected to contribute in improving the convergence educational activity, interest, curiosity, and inquiry ability of students in the universe and the astronomical bodies.