• Reproductive and Developmental Biology
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• 제35권1호
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• pp.67-75
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• 2011

The faulty regulation of imprinting gene lead to the abnormal development of reconstructed embryo after nuclear transfer. However, the correlation between the imprinting status of donor cell and preimplantation stage of embryo development is not yet clear. In this study, to determine this correlation, we used the porcine spermatogonial stem cell (pSSC) and fetal fibroblast (pFF) as donor cells. As the results, the isolated cells with laminin matrix selection strongly expressed the GFR ${\alpha}$-1 and PLZF genes of SSCs specific markers. The pSSCs were maintained to 12 passages and positive for the pluripotent marker including OCT4, SSEA1 and NANOG. The methylation analysis of H19 DMR of pSSCs revealed that the zinc finger protein binding sites CTCF3 of H19 DMRs displayed an androgenic imprinting pattern (92.7%). Also, to investigate the reprogramming potential of pSSCs as donor cell, we compared the development rate and methylation status of H19 gene between the reconstructed embryos from pFF and pSSC. This result showed no significant differences of the development rate between the pFFs ($11.2{\pm}0.8%$) and SSCs ($13.3{\pm}1.1%$). However, interestingly, while the CTCF3 methylation status of pFF-NT blastocyst was decreased (36.3%), and the CTCF3 methylation status of pSSC-NT blastocyst was maintained. Therefore, this result suggested that the genomic imprinting status of pSSCs is more effective than that of normal somatic cells for the normal development because the maintenance of imprinting pattern is very important in early embryo stage.

• 식물병연구
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• 제26권2호
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• pp.88-94
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• 2020

Recently, the severe dieback of apple trees resulting from soil-borne diseases has occurred in South Korea. The casual agents of dieback were surveyed on 74 apple orchards that had been damaged nationwide in 2016-2019. The number of apple orchards affected alone by Phytophthora rot, violet root rot, and white root rot was 31, 34, and 3, respectively. Also, the total number of mixed infection orchards was 6. Out of 9,112 apple trees affected by dieback, the trees damaged by Phytophthora rot, violet root rot, and white root rot were 3,332, 3,831, and 44, respectively. Moreover, the total number of mixed infection apple trees was 1,905. The provinces mainly affected were Gyeongnam, Gyeongbuk, Chungbuk, and Jeonbuk. The survey on these infected apple orchards will be available to form management strategy for the dieback that had been increased by soil-borne fungal pathogens.

• 농업과학연구
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• 제49권4호
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• pp.749-760
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• 2022

Omics-driven synthetic biology is a multidisciplinary research field that creates new artificial life by employing genetic components, biological devices, and engineering technique based on genetic knowledge and technological expertise. It is also utilized to make valuable biomaterials with limited production via current organisms faster, more efficient, and in huge quantities. As the bioeconomic age begins, and the global synthetic biology market becomes more competitive, investment in research and development (R&D) and associated sectors has grown considerably. By overcoming the constraints of present biotechnologies through the merging of big data and artificial intelligence technologies, huge ripple effects are envisaged in the pharmaceutical, chemical, and energy industries. In agriculture, synthetic biology is being used to solve current agricultural problems and develop sustainable agricultural systems by increasing crop productivity, implementing low-carbon agriculture, and developing plant-based, high-value-added bio-materials such as vaccines for diagnosing and preventing livestock diseases. As international regulatory debates on synthetic biology are now underway, discussions should also take place in our country for the growth of bioindustries and the dissemination of research findings. Furthermore, the system must be improved to facilitate practical application and to enhance the risk evaluation technology and management system.

• 한국패류학회지
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• 제25권1호
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• pp.23-28
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• 2009

2006년 6월부터 11월까지 매월 1회씩 제주도 귀덕, 신산, 김녕, 강정 연안에서 정치망에 의해 채집된 창꼴뚜기를 대상으로 창꼴뚜기의 생식생물학적 특성에 관하여 연구하였다. 조사기간 동안 총 759 개체의 창꼴뚜기 중에서 암컷은 429 개체, 수컷은 330 개체로 나타났다. 암컷의 경우, 조사 기간 중 미숙, 중숙, 성숙, 완숙기의 개체들이 혼재하여 출현하였으며 산란이 가능한 완숙 개체는 조사기간 중 초기인 6월을 제외하고 모든기간에 걸쳐 관찰되었고, 11월에 출현비율이 가장 높았다. 수컷도 조사기간 중 미숙, 중숙, 성숙, 완숙기의 개체들이 혼재하여 출현하됐으며 생식소의 숙도가 방정이 가능한 성숙단계이상의 개체는 전 기간에 걸쳐 관찰되었고 완숙기의 개체는 암컷과 같이 11월에 출현비율이 가장 높았다. 창꼴뚜기가 산란에 참여하는 군성숙외투장을 알아본 결과 성숙외투장은 17.1cm 로 추정되었다.

• 한국지능정보시스템학회:학술대회논문집
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• 한국지능정보시스템학회 2005년도 공동추계학술대회
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• pp.425-428
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• 2005

Healthcare is a research field suitable for applying the recent ubiquitous techniques. As a test system, we developed a kind of CDSS (Clinical Decision Support System) running in ubiquitous environment. called as 'uCDSS'. The uCDSS is a core system of the ubiquitous healthcare and is composed of some 'uMLMs(Ubiquitous Medical Logic Modules)'. The uMLMs based on the class in C# programming language could be reused in development of CDSS, or another EHR system running in .NET environment. As a test system, we developed the DM(Diabetes Mellitus knowledge system using ASP.NET. This system shows the potential of C# class-based uMLMs and the extensibility to any .NET development project.

• 한국식품과학회지
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• 제32권2호
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• pp.312-316
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• 2000

Xylan과 xylanase의 효소반응 후 생성되는 crude xylooligosaccharides를 효과적으로 정제하기 위하여 한외여과를 실시하였다. 한외여과의 최적운전 조건을 확인하고자 5, 10, 20, 30 psi의 막 횡단 압력에서 유속(100, 200, 300, 400 ml/min) 및 온도(10, 20, 23-25, $30^{\circ}C$)를 변화시키며 실험을 실시하였고 시간에 따른 flux의 변화를 관찰하였다. 또한 한외여과 효과를 알아보기 위해 한외여과 전, 후의 이화학적 특성 및 올리고당을 분석하였다. 유속과 온도에 따른 flux 변화를 관찰하여 시료의 최적 유속(300 ml/min), 최적 막 횡단 압력(20psi) 및 운전 온도$(23-25^{\circ}C)$를 결정하였다. 최적운전 조건에서 시간에 따른 flux 변화는 한외여과 종료시점인 VCR 10.0에서 초기 flux의 62% 수준의 flux를 나타냈다. 한외여과에 따른 일반성분의 변화는 당농도, 고형분, 총당 및 회분에서는 차이를 보이지 않았으나 단백질의 경우에는 여과 후 55%의 제단백 효과가 있었다. 한외여과 전, 후의 xylooligosaccharides함량 변화는 관찰할 수 없었으며 한외여과 여부가 올리고당 함량에는 영향을 미치지는 않는 것으로 생각한다. 시간에 따른 색도의 변화는 한외여과 전 시료 색도(1.0454 at 420 nm)와 한외여과 후 색도를 비교하면 평균 84%(0.1648 at 420 nm) 제거할 수 있는 결과를 얻었으며 한외여과 방법이 crude xylooligosaccharide의 탈색에 매우 효과적인 방법임을 확인할 수 있었다.

• Osong Public Health and Research Perspectives
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• 제9권6호
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• pp.314-324
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• 2018

Objectives: The aim of this study was to analyze research and development projects in mental health services in Korea, using priority evaluation of mental health promotion policies to determine direction of the service. Methods: An online survey was conducted that targeted experts in the mental health service regarding promotion of mental health in Korea in 2016. The survey was based on 32 policy projects that resulted from 12 strategies according to 4 policy objectives. Results: Analysis of 32 mental health projects were assessed regarding the possibility of technology development success, magnitude of the ripple effect, and necessity of a national response. It was observed that 3 policy projects relevant to suicide, had a high relative priority. This was followed by policies for improvement of health insurance and the medical benefit cost system, and policies for reinforcement of crisis psychological support such as those for disaster victims. Conclusion: The prioritization of mental health services should place an emphasis on promotion of a healthy mental lifestyle, rehabilitation support for patients with serious mental illness, and reinforcement of social safety networks for suicide prevention.

• Archives of Pharmacal Research
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• 제27권1호
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• pp.86-93
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• 2004

Nitric oxide (NO) has emerged as an important intracellular and intercellular messenger, controlling many physiological processes and participating in the fertilization process via the autocrine and paracrine mechanisms. This study investigated whether nitric oxide synthase (NOS) inhibitior (L-NAME) and L-arginine could regulate in vitro fertilization and early embryonic development in mice. Mouse epididymal spermatozoa, oocytes, and embryos were incubated in mediums of variable conditions with and without L-NAME or L-arginine (0.5, 1, 5 and 10mM). Fertilization rate and early embryonic development were significantly inhibited by treating sperms or oocytes with L-NAME (93.8% vs 66.3%,92.1% vs 60.3%), but not with L-arginine. In contrast, fertilization rate and early embryonic development were conspicuously reduced when L-NAME or L-arginine was added to the culture media for embryos. Early embryonic development was inhibited by microinjection of L-NAME into the fertilized embryosin a dose-dependent manner, but only by high concentrations of L-arginine. These results suggest that a moderate amount of NO production is essential for fertilization and early embryo development in mice.

• 한국초전도학회:학술대회논문집
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• 한국초전도학회 2002년도 High Temperature Superconductivity Vol.XII
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• pp.71-71
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• 2002

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2002년도 International Meeting on Information Display
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• pp.901-904
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• 2002

Display performance of mobile LCDs has been improved according to broadening of their application. But there are many demands from market, such as contrast improvement (high color reproducibility), higher efficiency of light usage, thickness reduction, widening its viewing angle and so on. To respond them we have been developing many new types of films. In this paper we introduce new high contrast white polarizers for better color reproducibility, and new film construction for higher light efficiency.