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Considerations and Alternative Approaches to the Estimation of Local Abundance of Legally Protected Species, the Fiddler Crab, Austruca lactea (법정보호종, 흰발농게(Austruca lactea) 서식 개체수 추정에 대한 검토와 대안)

  • Yoo, Jae-Won;Kim, Chang-Soo;Park, Mi-Ra;Jeong, Su-Young;Lee, Chae-Lin;Kim, Sungtae;Ahn, Dong-Sik;Lee, Chang-Gun;Han, Donguk;Back, Yonghae;Park, Young Cheol
    • Journal of Wetlands Research
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    • v.23 no.2
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    • pp.122-132
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    • 2021
  • We reviewed the methods employed in Korean tidal flat surveys to measure the local abundance of the endangered wildlife and marine protected species, the fiddler crab, Austruca lactea. A complete census for infinite population is impossible even in a limited habitat within a tidal flat, and density estimates from samples strongly vary due to diverse biological and ecological factors. The habitat boundaries and areas shift with periodicities or rhythmic activities of organisms as well as measurement errors. Hence the local abundance calculated from density and habitat areas should be regarded as transient. This conjecture was valid based on the spatio-temporal variations of the density averages, standard error ranges, and spatial distribution of the crab, A. lactea observed for 3 years (2015-2017) in Songdo tidal flat in Incheon. We proposed the potential habitat areas using the occurrence probability of 50% from logistic regression model, reflecting the importance of habitat conservation value as an alternative to local abundance. The spatial shape of potential habitat predicted from a generalized model would remain constant over time unless the species' critical environmental conditions change rapidly. The species-specific model is expected to be used for the introduction of desired species in future habitat restoration/creation projects.

The flora of vascular plants in Deoksesan Mt. and its adjacent areas (Inje, Gangwon) (덕세산(인제, 강원) 일대의 관속식물상)

  • KIM, Kyung-Ah;GO, Ah-Reum;LEE, Yun-Sun;CHEON, Kyeong-Sik;YOO, Ki-Oug
    • Korean Journal of Plant Taxonomy
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    • v.51 no.2
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    • pp.147-165
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    • 2021
  • The flora of the vascular plants in Deoksesan Mt. and its adjacent areas, located at Inje-gun, Gangwon province, were surveyed for a total ten times from April of 2018 to July of 2020. The result of this survey revealed 457 taxa, consisting of 90 families, 287 genera, 404 species, 13 subspecies, 35 varieties, and 5 forms. Among them, 12 taxa were endemic plants to Korea, and 7 taxa were rare plants. Red list plants and those designated as controlled Korean biological resources, requiring special permission for export outside of the county, numbered 3 and 103 taxa, respectively. The floristic target species amounted to 83 taxa, specifically 8 taxa of grade IV, 26 taxa of grade III, 21 taxa of grade II, and 28 taxa of grade I. In addition, 14 taxa were classified as plants adaptable to climate change, and 4 taxa at their respective northern limits, first recorded in this study, were also investigated. Twenty-nine taxa of alien plants and 4 taxa of ecosystem disturbance species were also found in this area. Our results suggest that some species should be preserved and can serve as basic information with which to establish data on the preservation of resources, such as plant diversity and distributional changes on Deoksesan Mt.

A study on the risk assessment system for the harmful marine species: the legal problems and solutions (유해해양생물 위해성평가 제도의 문제점 및 개선방안 연구)

  • Lee, Chang Su;Moh, Youngdawng
    • Korean Journal of Environmental Biology
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    • v.38 no.4
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    • pp.691-704
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    • 2020
  • The Ministry of Oceans and Fisheries has designated 17 species as harmful marine organisms with the purpose of managing harmful marine species that threaten health and property. In designating and managing harmful marine species, detailed and effective regulations were originally established in November 2015, and a minor amendment of the directive was published in 2019 - Directive on Designating and Managing Marine Ecosystem Invasive Species and Harmful Marine Species (hereinafter, the Directive). Thus, this study had two aims: Firstly, to increase public awareness of the harmful marine species management system run by the Ministry of Oceans and Fisheries via description of the present harmful marine species risk assessment system. Secondly, to improve the current risk assessment system by providing policy suggestions developed through review of the present harmful marine species designation and management system. In so doing, this study reviewed the 'harmful marine species - harmfulness risk assessment system' in both the definitions of "risk" and "assessment". As a result, the present definition of 'risk' adequately fulfills the policy aims on the Regulation of Marine Ecosystem, which includes an economic value. However, it seems that there is a loophole in the rules of risk assessment, lacking terms of reference in the definition of "risk". Moreover, with regards to risk assessment, a quantitative risk assessment system was sufficient but lacked elements of qualitative risk, suggesting future research in this area may prove useful in the management of harmful marine species.

Supplement of High Protein-Enriched Diet Modulates the Diversity of Gut Microbiota in WT or PD-1H-Depleted Mice

  • Xie, Yajun;Zhao, Ping;Han, Zhigang;Li, Wei;Shi, Dan;Xu, Lei;Yi, Qiying
    • Journal of Microbiology and Biotechnology
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    • v.31 no.2
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    • pp.207-216
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    • 2021
  • Supplement of high-protein food plays an important role in improving the symptoms of malnutrition and the immune capacity of the body, but the association of high-protein diet and gut microbiota remained unaddressed. Here, we systematically analyzed the internal organs and gut microbiota in C57(WT) or PD-1H-depleted (KO) mice (T cells were activated) fed with pupae or feed for six weeks. We observed that the body weight gain in the mice fed with pupae increased less significantly than that of the feed group, while the villi and small intestine lengths in the pupa group were reduced compared with that of mice given feed. However, the average body weight of the KO mice increased compared with that of the WT mice fed with pupae or feed. Pupae increased the concentration of blood glucose in WT, but not in KO mice. Moreover, in the feed group, there was no difference in the weight of the internal organs between the WT and KO mice, but in the pupae-fed group, liver weight was decreased and spleen weight was increased compared with that of KO mice. The amounts/plural/amounts of Melainabacteria, Chloroflexi, and Armatimonadetes were specifically upregulated by pupae, and this upregulation was weakened or eliminated by PD-1H depletion. Some bacteria with high abundance in the feed-fed KO mice, such as Deferribacteres, Melainabacteria, Acidobacteria, Bacteroidetes, Spirochaetes and Verrucomicrobia, were decreased in pupae-fed KO mice, and Proteobacteria and Deinococcus were specifically enriched in pupae-fed KO mice. Bacteroidetes, Firmicutes and Akkermansia were associated with weight loss in the pupae-fed group while Lachnospiraceae and Anaerobiospirillum were related glucose metabolism and energy consumption. Based on high-throughput sequencing, we discovered that some gut bacteria specifically regulated the metabolism of a high-protein diet, and PD-1H deficiency improved life quality and sustained blood glucose. Moreover, PD-1H responses to high-protein diet through modulating the type and quantity of gut bacteria. These findings provide evidence about the association among gut microbiota, T cell activation (for PD-1H depletion) and high-protein diet metabolism, have important theoretical significance for nutrition and health research.

Forest Vegetation Structure around Marugeum (Ridge Line) Area in Deogyusan Region, Baekdudaegan (백두대간 덕유산권역 마루금 주변의 산림식생구조)

  • Kim, Hojin;Song, Juhyeon;Lee, Jeongeun;Cho, Hyunje;Park, Wangeun;Kim, Sujin;Yun, Chung Weon
    • Journal of Korean Society of Forest Science
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    • v.111 no.1
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    • pp.81-99
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    • 2022
  • The Baekdudaegan, spanning around 701 km from Hyangrobong to Cheonwangbong of Mt. Jirisan in South Korea, is rich in biodiversity and known as the main ecological axis of the Korean Peninsula. The Neuljae-Yuksimnyeong section of Marugeum in the southern part of the Baekdudaegan, particularly Mt. Deogyusan, is an area in which various types of vegetation appear depending on the environmental characteristics. The aims of this study were to investigate the current vegetation status at the Neuljae-Yuksimnyeong section of the Baekdudaegan and to provide basic data to aid systematic conservation and management through future classification of forest vegetation types. A vegetation survey was conducted using 637 plots from May to October 2019. Vegetation-type analysis showed that the vegetation units could be classified as a Quercus mongolica community group divided into seven communities: the Abies koreana community, Sanguisorba hakusanensis community, Persicaria hydropiper community, Quercus variabilis community, Quercus dentata community, Cornus controversa community, and Quercus mongolica community. The A. koreana community was subdivided into the Dryopteris expansa group and Picea jezoensis group. The Q. variabilis community was also subdivided into the Q. dentata group and Q. variabilis typical group. We concluded that special management plans for distinctive forest vegetation, including subalpine vegetation, grass or herb vegetation, and agricultural vegetation, should be prepared urgently to aid ecosystem preservation and enhancement.

Understanding Over The Top(OTT) and Continuance Intention to Use OTT: Impacts of OTT Characteristics and Price Fairness (Over The Top(OTT)의 지속이용의도에 대한 이해: OTT 특성과 가격공정성의 영향)

  • Park, Hyunsun;Kim, Sanghyun;Sohn, Changyong
    • Knowledge Management Research
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    • v.23 no.1
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    • pp.203-225
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    • 2022
  • Competition in the OTT (Over the Top) service market is getting fiercer since global OTT services enter the domestic market and existing platforms are actively reorganized. As powerful competitors with ultra-luxurious content continue to enter the marke with diversity required by users, various efforts are required for OTT service platforms to prevent subscriber churn in order to generate continuous revenue. Thus, this study tried to examine the effect of OTT service characteristics on continuous use intention through an empirical analysis based on Expectation-Confirmation Model(ECM). A total of 386 responses were collected from individuals who have experience or are currently using OTT service and analyzed using AMOS 24. Results show that content curation, content richness, and audience activity had a significant effect on expectation confirmation. Also, expectation confirmation had a significant effect on perceived usefulness and user satisfaction while perceived usefulness had a significant effect on user satisfaction, significantly influencing continuous intention to use OTT. Finally, price fairness was found to strengthen all proposed relationships. The findings are expected to provide useful information for service and content development for subscriber retention, which has the most direct impact on revenue generation of OTT service providers.

Supplementation of Microalgae (Tetradesmus sp.) to Pre-Starter Diet for Broiler Chickens (초기 육계 사료 내 미세조류(Tetradesmus sp.) 첨가에 따른 장관환경 변화)

  • Rim, Chae Yun;Jung, Hui-su;An, Su Hyun;Joo, Sang Seok;Kim, Z-Hun;Kong, Changsu;Kim, Myunghoo
    • Korean Journal of Poultry Science
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    • v.49 no.2
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    • pp.125-137
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    • 2022
  • This study aimed to determine the effects of dietary microalgae (Tetradesmus sp. (TO)) on intestinal immunity and microbiota of pre-starter broilers. One hundred and twenty 1-day-old birds (Ross 308) were allocated to two dietary treatment groups with six blocks in a randomized complete block design. The two experimental diets consisted of a corn-soybean meal-based basal diet and a diet with 0.5% TO powder instead of cornstarch in the basal diet. After feeding the experimental diets for ten days, all birds' body weight and feed intake were measured, and representative eight birds were selected from each treatment group. Small intestinal lamina propria cells were isolated using flow cytometry to examine the frequency of immune cells. Cecal feces were harvested for 16s rRNA gut microbiota analysis and fecal IgA levels. Here, we found that 0.5% TO supplementation increased CD3+CD4+ T cells in the small intestine, but decreased CD3+CD8+ T cells in the small intestine. Gut microbial analysis showed that TO supplementation significantly increased the alpha diversity of the gut microbiome. Taxonomic analysis showed that TO treatment increased the abundance of Firmicutes and decreased that of Bacteroidetes at the phylum level. The distribution of Enterobacteriaceae containing many harmful bacteria at the family level, was lower in the TO group. In the LEfSe analysis, the TO group had a significantly enriched abundance of Agathobaculum at the genus level. Overall, results show that Tetradesmus sp. supplementation influences intestinal T-cell immunity and induces the expansion of beneficial gut microbes in pre-starter broiler chickens.

Verification of Entertainment Utilization of UAS FC Data Using Machine Learning (머신러닝 기법을 이용한 무인항공기의 FC 데이터의 엔터테인먼트 드론 활용 검증)

  • Lee, Jae-Yong;Lee, Kwang-Jae
    • Journal of Korea Entertainment Industry Association
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    • v.15 no.4
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    • pp.349-357
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    • 2021
  • Recently, drones are rapidly becoming common and expanding. There is a great need for diversity in whether drone flight data can be used as entertainment technology analysis data. In particular, it is necessary to check whether it is possible to analyze and utilize the flight and operation process of entertainment drones, which are developing through autonomous and intelligent methods, through data analysis and machine learning. In this paper, it was confirmed whether it can be used as a machine learning technology by using FC data in the evaluation of drones for entertainment. As a result, FC data from DJI and Parrot such as Mavic2 and Anafi were unable to analyze machine learning for entertainment. It is because data is collected at intervals of 0.1 second or more, so that it is impossible to find correlation with other data with GCS. On the other hand, it was found that machine learning technologies can be applied in the case of Fixhawk, which used an ARM processor and operates with the Nuttx OS. In the future, it is necessary to develop technologies capable of analyzing the characteristics of entertainment by dividing fixed-wing and rotary-wing flight information. For this, a model shoud be developed, and systematic big data collection and research should be conducted.

Analysis of 16S rRNA gene sequencing data for the taxonomic characterization of the vaginal and the fecal microbial communities in Hanwoo

  • Choi, Soyoung;Cha, Jihye;Song, Minji;Son, JuHwan;Park, Mi-Rim;Lim, Yeong-jo;Kim, Tae-Hun;Lee, Kyung-Tai;Park, Woncheoul
    • Animal Bioscience
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    • v.35 no.11
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    • pp.1808-1816
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    • 2022
  • Objective: The study of Hanwoo (Korean native cattle) has mainly been focused on meat quality and productivity. Recently the field of microbiome research has increased dramatically. However, the information on the microbiome in Hanwoo is still insufficient, especially relationship between vagina and feces. Therefore, the purpose of this study is to examine the microbial community characteristics by analyzing the 16S rRNA sequencing data of Hanwoo vagina and feces, as well as to confirm the difference and correlation between vaginal and fecal microorganisms. As a result, the goal is to investigate if fecal microbiome can be used to predict vaginal microbiome. Methods: A total of 31 clinically healthy Hanwoo that delivered healthy calves more than once in Cheongju, South Korea were enrolled in this study. During the breeding season, we collected vaginal and fecal samples and sequenced the microbial 16S rRNA genes V3-V4 hypervariable regions from microbial DNA of samples. Results: The results revealed that the phylum-level microorganisms with the largest relative distribution were Firmicutes, Actinobacteria, Bacteroidetes, and Proteobacteria in the vagina, and Firmicutes, Bacteroidetes, and Spirochaetes in the feces, respectively. In the analysis of alpha, beta diversity, and effect size measurements (LefSe), the results showed significant differences between the vaginal and fecal samples. We also identified the function of these differentially abundant microorganisms by functional annotation analyses. But there is no significant correlation between vaginal and fecal microbiome. Conclusion: There is a significant difference between vaginal and fecal microbiome, but no significant correlation. Therefore, it is difficult to interrelate vaginal microbiome as fecal microbiome in Hanwoo. In a further study, it will be necessary to identify the genetic relationship of the entire microorganism between vagina and feces through the whole metagenome sequencing analysis and meta-transcriptome analysis to figure out their relationship.

The Association of Auditive and Visual Meanings in (영화 <이미테이션 게임>에 나타난 청각적·시각적 의미의 연합)

  • Ahn, Soo Hwan
    • The Journal of the Korea Contents Association
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    • v.21 no.7
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    • pp.83-92
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    • 2021
  • The goal of this paper is to explore the relationship between visual and auditive meanings of the film "The Imitation Game"(2013) which is directed by Morten Tyldum, and with music composed by Alexandre Desplat. The author accepted Zwikowski's idea of analyzing how film music and visual information created a chemical and meaningful signal for the audience. Lawrence Zbikowski has used Conceptual Integration Networks (CIN), devised by Golles Fauconnier and Mark Turner, to analyze latent meanings which was produced by the association of visual and auditive meanings. Therefore, The author applied the CIN analyzing methodology to the research combination of music and imagery in "The Imitaion Game." Desplat, the composer, has used aeolian instead of tonality to represent the main character. The aeolian melodic line was changed to create a similar atmosphere of imagery and narratives. The irregular time and minor chord was associated with unstable emotion and familiar intervals, while the major chord was associated with stable feelings. Desplat also applied instrumental diversity and extreme changes of dynamic to create positive or negative cognition. The author, therefore, found how the meanings of auditive materials and visual information combined and emphasized encoded messages in the film.