• Journal of Life Science
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• 제27권6호
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• pp.661-672
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• 2017

Endophytic fungi are present in host plants and contribute to resistance to biotic and abiotic stress. Aquatic plants are living in aquatic environment such as saltwater or freshwater and exposed more water stress than other land plants. In this study, we investigated 4 wetlands in Jeju and collected 11 aquatic plants. Exogenous microbes were removed by preprocessing of plants and endophytic fungal strains were isolated from the plants. We isolated 126 fungal strains from Namsaengi-pond, 22 fungal strains from Sujangdong-marsh, 44 fungal strains from Yongsu-reservoir and 32 fungal strains from Gangjeongcheon. The fungal strains were identified using internal transcribed spacer (ITS) region and analyzed the phylogeny and diversity. Endophytic fungi isolated from plants of Namsaengi-pond were classified to 30 genera, 19 families, 12 orders, 7 classes and 4 phyla. Endophytic fungi of Sujangdong-marsh were classified to 11 genera, 11 families, 6 orders, 5 classes and 4 phyla. Endophytic fungi of Yongsu-reservoir were classified to 13 genera, 12 families, 7 orders, 5 classes and 4 phyla. Endophytic fungi isolated from Gangjeongcheon were classified to 9 genera, 7 families, 5 orders, 2 classes and 1 phyla. Overall, they were divided 40 genera and Alternaria, Colletotrichum and Fusarium were isolated from 4 sites in common. By investigating the endophytic fungi in aquatic plants, it is for baseline data that determination of diversity and the ecological distribution of endophytic fungi.

• Korean Journal of Ecology and Environment
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• 제53권2호
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• pp.173-184
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• 2020

Irrigation ponds, 'dumbeong', which are artificially constructed water resources for traditional farming, serve as a biological shelter connecting seasonally created rice paddy fields to local freshwater ecosystems. This 2018 study surveyed 143 irrigation ponds in the western Civilian Control Zone (CCZ) area from August to September, revealing species diversity and community characteristics of benthic macroinvertebrates. A total of 13,454 individuals of macroinvertebrates were captured and classified into 3 phyla, 5 classes, 17 orders, 59 families, 192 species. Among Insecta, the most frequently recorded order was Odonata, 55 spp.(33.7%), followed by Coleoptera, 52 spp. (31.9%), Hemiptera, 34 spp. (20.8%), Diptera, 17 spp. (9.8%), Ephemeroptera, 3 spp. (2.4%), Trichoptera, 1 spp. (0.6%) and Lepidoptera, 1 spp. (0.6%). Taxon of non-Insecta consisted of Mollusca, 14 spp. (48.2%), Annelida, 11 spp. (37.9%) and Arthropoda, 4 spp. (3.4%). The analysis of Diversity Index (H'), Species Richness Index (RI), Dominance Index (DI) and Evenness Index (J') revealed the general stability of communities in the study sites. A total of 28 rare species were found in 98 study sites, including three endangered species designated by the Ministry of Environment. These results showed that the species diversity and rarity of macroinvertebrates in the study area were greater than those of previous research on lentic wetlands (lake, etc.) and national conserved wetlands(Upo-swamp, etc.) in Korea. A conservation planning of aquatic ecosystems in the western CCZ area, therefore, should focus on conservation of irrigation ponds.

• The Korean Journal of Ecology
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• 제23권2호
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• pp.131-134
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• 2000

Forest vegetation in Korea can be largely divided into warm temperate, cool temperate and frigid forest zone. The cool temperate forest zone of them occupies the largest part of the Korean peninsula and it is generally divided into three subdivisions such as northern, central and southern subzone. The Forestry Research Institute established three long-term ecological research sites at Kwangnung Experiment Forest in the central subzone of the cool temperate forest zone, at the Mt. Kyebangsan Forest in the northern subzone of the cool temperate forest zone. and at the Mt. Keumsan Forest in the warm temperate forest zone. The objectives of long-term ecological research in the Forestry Research Institute, Korea are to study long-term changes of the forest ecosystems in energy fluxes, water and nutrient cycling, forest stand structure, biological diversity, to quantify nutrient budgets and fluxes among forest ecosystem compartments and to integrate ecological data with a GIS - assisted model. To achieve the objectives, forest stand dynamics. environmental changes in soil properties, stream water quality, nutrient cycling, air pollution and biological diversity have been investigated and plant phonology as an indicator of climate change has been monitored in the LTER sites.

• ETRI Journal
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• 제44권6호
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• pp.1004-1019
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• 2022

For autonomous driving without high-definition maps, we present a model capable of generating multiple plausible paths from egocentric images for autonomous vehicles. Our generative model comprises two neural networks: feature extraction network (FEN) and path generation network (PGN). The FEN extracts meaningful features from an egocentric image, whereas the PGN generates multiple paths from the features, given a driving intention and speed. To ensure that the paths generated are plausible and consistent with the intention, we introduce an attentive discriminator and train it with the PGN under a generative adversarial network framework. Furthermore, we devise an interaction model between the positions in the paths and the intentions hidden in the positions and design a novel PGN architecture that reflects the interaction model for improving the accuracy and diversity of the generated paths. Finally, we introduce ETRIDriving, a dataset for autonomous driving, in which the recorded sensor data are labeled with discrete high-level driving actions, and demonstrate the state-of-the-art performance of the proposed model on ETRIDriving in terms of accuracy and diversity.

• Asian-Australasian Journal of Animal Sciences
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• 제26권2호
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• pp.163-170
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• 2013

In order to analyze the genetic diversity and phylogenetic status of the Korean Chikso breed, we determined sequences of mtDNA cytochrome b (cyt b) gene and performed phylogenetic analysis using 239 individuals from 5 Chikso populations. Five non-synonymous mutations of a total of 15 polymorphic sites were identified among 239 cyt b coding sequences. Thirteen haplotypes were defined, and haplotype diversity was 0.4709 ranging from 0.2577 to 0.6114. Thirty-five haplotypes (C1-C35) were classified among 9 Asia and 3 European breeds. C2 was a major haplotype that contained 206 sequences (64.6%) from all breeds used. C3-C13 haplotypes were Chikso-specific haplotypes. C1 and C2 haplotypes contained 80.5% of cyt b sequences of Hanwoo, Yanbian, Zaosheng and JB breeds. In phylogenetic analyses, the Chikso breed was contained into B. taurus lineage and was genetically more closely related to two Chinese breeds than to Korean brown cattle, Hanwoo. These results suggest that Chikso and Hanwoo have a genetic difference based on the mtDNA cyt b gene as well as their coat color, sufficient for classification as a separate breed.

• The Korean Journal of Quaternary Research
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• 제13권1호
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• pp.91-98
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• 1999

The effect of the relative abundances of planktonic foraminifera juveniles on the climatic and oceanographic history interpretations is documented for the first time (as far as we know) by use of the year-long time-series sediment trap samples. Statistical correlation analysis suggests that many climatic and oceanographic variables such as sinking flux (total number) of planktonic foraminifera, relative abundance of some climatic indicator species G. bulloides and N. dutertrei, temperature, and salinity do not show any significant correlations with the relative abundance of planktonic foraminifera juveniles. However, planktonic foraminifera juveniles show moderate correlations with species diversity indices (species richness, Shannon-Wiener index, and Equitability). These indicate that the juveniles do not affect the relative abundances(%) of species compositions but affect the species diversity indices. Based on this one-year trap result, special care is required when we use species diversity indices for the interpretations of paleoceanography when the juveniles are excluded from total planktonic foraminiferal countings.

• Korean Journal of Veterinary Research
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• 제43권1호
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• pp.77-86
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• 2003

Pullorum disease due to Salmonella enterica subspecies enterica bioserovar Pullorum (S. pullorum) is reported to be an endemic disease in domestic poultry flocks. The pulsed-field gel electrophoresis (PFGE) subtyping method was used to assess the extent of genetic diversity and clonality of most of salmonella serotypes and other diverse bacterial species from animals and environmental samples in worldwide. Nowadays, PFGE has already been evaluated as a gold standards for molecular subtyping of salmonella serotypes compared with other molecular analysis methods. PFGE of XbaI digested chromosomal DNA from 23 strains of S. pullorum gave 5 distinctive pulsotypes (from SXPI to SXPV) with 5% confidence range of Dice coefficients, indicating that PFGE is very discriminative and that multiple clones of S. pullorum have been existed and diffused all of domestic poultry flocks industries since 1995. Two dominant pulsogroups (SXA & SXB) appeared as a major clones in this country, because they had consistently been recovered from diverse sources including both chicken organs and raw feed materials between 1995 and 1998. In addition, the matching percentage of PFGE profiles (PFP) among strains from both chickens and feed ingredients provides indirect evidence of the possible transmission of pullorum disease from contaminated raw feed ingredients for chicken production. In calculating of discrimination index (DI) for PFGE method by Simpson's index, DI was appeared as 0.917. Therefore, this index suggested that the present PFGE would seem to be a desirable and confident molecular typing method for S. pullorum strains. To our knowledge for pullorum disease, this is the first study to compare S. pullorum strains from chicken organs and feed samples using the PFGE.

• Parasites, Hosts and Diseases
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• 제53권3호
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• pp.341-344
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• 2015

Toxoplasma gondii is an opportunistic protozoan parasite that can infect almost all warm-blooded animals including humans with a worldwide distribution. Micronemes play an important role in invasion process of T. gondii, associated with the attachment, motility, and host cell recognition. In this research, sequence diversity in microneme protein 6 (MIC6) gene among 16 T. gondii isolates from different hosts and geographical regions and 1 reference strain was examined. The results showed that the sequence of all the examined T. gondii strains was 1,050 bp in length, and their A + T content was between 45.7% and 46.1%. Sequence analysis presented 33 nucleotide mutation positions (0-1.1%), resulting in 23 amino acid substitutions (0-2.3%) aligned with T. gondii RH strain. Moreover, T. gondii strains representing the 3 classical genotypes (Type I, II, and III) were separated into different clusters based on the locus of MIC6 using phylogenetic analyses by Bayesian inference (BI), maximum parsimony (MP), and maximum likelihood (ML), but T. gondii strains belonging to ToxoDB #9 were separated into different clusters. Our results suggested that MIC6 gene is not a suitable marker for T. gondii population genetic studies.

• Fisheries and Aquatic Sciences
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• 제26권9호
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• pp.569-581
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• 2023

The morphology and genetic identification of Rasbora lateristriata and Rasbora argyrotaenia between cultivated and wild populations has never been reported. This study compares morphology and cytochrome c oxidase (COI) genes between farmed and wild stock Rasbora spp. in Java and Sumatra island, Indonesia. We analyzed the truss network measurement (TNM) characters of 80 fish using discriminant function analysis statistical tests. DNA was extracted from muscle tissue of 24 fish specimens, which was then followed by polymerase chain reaction, sequencing, phylogenetic analysis, fixation index analysis, and statistical analysis of haplotype networks. Basic Local Alignment Search Tool analysis validated the following species: R. lateristriata and R. argyrotaenia from farming (Jogjakarta); Rasbora agryotaenia (Purworejo), R. lateristriata (Purworejo and Malang), Rasbora dusonensis (Palembang), and Rasbora einthovenii (Riau) from natural resources. Based on TNM characters, Rasbora spp. were divided into four groups, referring to four distinct characters in the middle of the body. The phylogenetic tree is divided into five clades. The genetic distance between R. argyrotaenia (Jogjakarta) and R. lateristriata (Malang) populations (0.66) was significantly different (p < 0.05). R. lateristriata (Purworejo) has the highest nucleotide diversity (0.43). R. argyrotaenia from Jogjakarta and Purworejo shared the same haplotype. The pattern of gene flow among them results from the two populations' close geographic proximity and environmental effects. R. argyrotaenia had low genetic diversity, therefore, increasing heterozygosity in cultivated populations is necessary to avoid inbreeding. Otherwise, R. lateristriata (Purworejo) had a greater gene variety that could be used to develop breeding. In conclusion, the middle body parts are a distinguishing morphometric character of Rasbora spp., and the COI gene is more heterozygous in the wild population than in farmed fish, therefore, enrichment of genetic variation is required for sustainable Rasbora fish farming.

• Journal of Species Research
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• 제12권4호
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• pp.341-354
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• 2023

This is the third series of catalogs reporting on Korean species discovered through the 'Discovery of Korean Indigenous Species'. This catalog includes 22 species of invertebrates, excluding insects. The catalog includes the scientific name, an abridged list of synonyms, collection sites, distribution, diagnosis, and figures for each species. Additionally, we provide the newly assigned Korean name, specimen voucher, and, if available, mitochondrial CO1 or 16S gene sequences of the species listed. All species identified and documented here will be officially listed on the 'National Species List of Korea', a database maintained by the National Institute of Biological Resources(NIBR).