• Title/Summary/Keyword: Research Diversity

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Genetic Diversity and Dye-Decolorizing Spectrum of Schizophyllum commune Population

  • Choi, Yongjun;Nguyen, Ha Thi Kim;Lee, Tae Soo;Kim, Jae Kwang;Choi, Jaehyuk
    • Journal of Microbiology and Biotechnology
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    • v.30 no.10
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    • pp.1525-1535
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    • 2020
  • Synthetic dyes are widely used in various industries and their wastage causes severe environmental problems while being hazardous to human health, leading to the need for eco-friendly degradation techniques. The split-gill fungus Schizophyllum commune, which is found worldwide, has the potential to degrade all components of the lignocellulosic biomass and is a candidate for the treatment of synthetic dyes. A systematic molecular analysis of 75 Korean and 6 foreign S. commune strains has revealed the high genetic diversity of this population and its important contribution to the total diversity of S. commune. We examined the dye decolorization ability of this population and revealed 5 excellent strains that strongly decolorized 3 dyes: Crystal Violet, Congo Red and Methylene Blue. Finally, comparison of dye decolorization ability and the phylogenetic identification of these strains generalized their genetic and physiological diversity. This study provides an initial resource for physiological and genetic research projects as well as the bioremediation of textile dyes.

Genetic Diversity and Differentiation of Colletotrichum spp. Isolates Associated with Leguminosae Using Multigene Loci, RAPD and ISSR

  • Mahmodi, Farshid;Kadir, J.B.;Puteh, A.;Pourdad, S.S.;Nasehi, A.;Soleimani, N.
    • The Plant Pathology Journal
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    • v.30 no.1
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    • pp.10-24
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    • 2014
  • Genetic diversity and differentiation of 50 Colletotrichum spp. isolates from legume crops studied through multigene loci, RAPD and ISSR analysis. DNA sequence comparisons by six genes (ITS, ACT, Tub2, CHS-1, GAPDH, and HIS3) verified species identity of C. truncatum, C. dematium and C. gloeosporiodes and identity C. capsici as a synonym of C. truncatum. Based on the matrix distance analysis of multigene sequences, the Colletotrichum species showed diverse degrees of intera and interspecific divergence (0.0 to 1.4%) and (15.5-19.9), respectively. A multilocus molecular phylogenetic analysis clustered Colletotrichum spp. isolates into 3 well-defined clades, representing three distinct species; C. truncatum, C. dematium and C. gloeosporioides. The ISSR and RAPD and cluster analysis exhibited a high degree of variability among different isolates and permitted the grouping of isolates of Colletotrichum spp. into three distinct clusters. Distinct populations of Colletotrichum spp. isolates were genetically in accordance with host specificity and inconsistent with geographical origins. The large population of C. truncatum showed greater amounts of genetic diversity than smaller populations of C. dematium and C. gloeosporioides species. Results of ISSR and RAPD markers were congruent, but the effective maker ratio and the number of private alleles were greater in ISSR markers.

Population genetic structure and genetic variability of the marbled sole Pleuronectes yokohamae on the coast of Gyeongsangnam-do, Korea

  • Lee, So-Jeong;Lee, So-Gwang;Gwak, Woo-Seok
    • Animal cells and systems
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    • v.16 no.6
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    • pp.498-505
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    • 2012
  • This study uses the mitochondrial DNA control region to identify the genetic diversity and population structure of the marbled soles (Pleuronectes yokohamae) that inhabit Jinhae Bay and Yokji Island in the nearby sea and the adjacent waters of Namhae, Hansan Island, and Jaran Bay. Direct sequencing of the PCR products revealed 379 bp sequences with 83 variable nucleotide sites, defining a total of 91 haplotypes. The haplotype diversity was high, ranging from $0.917{\pm}0.031$ to $0.983{\pm}0.008$, and nucleotide diversity ranged from $0.015{\pm}0.008$ to $0.024{\pm}0.012$. In addition, 48 haplotypes (52.7%) were unique. Pairwise $F_{ST}$ values were very low, with the maximum value occurring between PYH (Hansan Island) and PJI (Jinhae Bay) ($F_{ST}$ = 0.011). Therefore, no significant genetic differentiation was evident between any pair of sampling localities.

High Diversity Transceiver for Low Power Differentially Encoded OFDM System

  • Nadeem, Faisal;Zia, Muhammad;Mahmood, Hasan;Bhatti, Naeem;Haque, Ihsan
    • ETRI Journal
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    • v.38 no.1
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    • pp.90-99
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    • 2016
  • In this work, we investigate differentially encoded blind transceiver design in low signal-to-noise ratio (SNR) regimes for orthogonal frequency-division multiplexing (OFDM) signaling. Owing to the fact that acquisition of channel state information is not viable for short coherence times or in low SNR regimes, we propose a time-spread frequency-encoded method under OFDM modulation. The repetition (spreading) of differentially encoded symbols allows us to achieve a target energy per bit to noise ratio and higher diversity. Based on the channel order, we optimize subcarrier assignment for spreading (along time) to achieve frequency diversity of an OFDM modulated signal. We present the performance of our proposed transceiver design and investigate the impact of Doppler frequency on the performance of the proposed differentially encoded transceiver design. To further improve reliability of the decoded data, we employ capacity-achieving low-density parity-check forward error correction encoding to the information bits.

Genetic Diversity of avrBs-like Genes in Three Different Xanthomonas Species Isolated in Korea

  • Oh, Chang-Sik;Lee, Seung-Don;Heu, Sung-Gi
    • The Plant Pathology Journal
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    • v.27 no.1
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    • pp.26-32
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    • 2011
  • Plant-pathogenic bacteria including Xanthomonas spp. carry genetic diversity in composition of avirulence genes for interaction with their host plants. Previously, we reported genetic diversity of avirulence genes in X. axonopodis pv. glycines. In this study, we determined genetic diversity of five avirulence genes, avrBs1, avrBs2, avrBs3, avrBs4, and avrRxv, in three other Xanthomonas species isolated in Korea by genomic southern hybridization. Although Korean races of X. campestris pv. vesicatoria that were isolated from year 1995 to 2002 had the same avirulence gene patterns as those that already reported, there was race shift from race 3 to race 1 by acquisition of avrBs3 genes. X. campestris pv. campestris isolated from Chinese cabbage, but not from cabbage or radish, carried two avrBs3 genes, and one of them affected HR-eliciting ability of this bacterium in broccoli. X. oryzae pv. oryzae carried eight to thirteen avrBs3 gene homologs, and this bacterium showed dynamic changes of resistance patterns in rice probably by losing or obtaining avrBs3 genes. These results indicate that avrBs3 gene is more diverse in Xanthomonas spp. than other four avirulence genes and also host ranges of these bacteria can be easily changed by loss or acquisition of avrBs3 genes.

Deep sequencing of B cell receptor repertoire

  • Kim, Daeun;Park, Daechan
    • BMB Reports
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    • v.52 no.9
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    • pp.540-547
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    • 2019
  • Immune repertoire is a collection of enormously diverse adaptive immune cells within an individual. As the repertoire shapes and represents immunological conditions, identification of clones and characterization of diversity are critical for understanding how to protect ourselves against various illness such as infectious diseases and cancers. Over the past several years, fast growing technologies for high throughput sequencing have facilitated rapid advancement of repertoire research, enabling us to observe the diversity of repertoire at an unprecedented level. Here, we focus on B cell receptor (BCR) repertoire and review approaches to B cell isolation and sequencing library construction. These experiments should be carefully designed according to BCR regions to be interrogated, such as heavy chain full length, complementarity determining regions, and isotypes. We also highlight preprocessing steps to remove sequencing and PCR errors with unique molecular index and bioinformatics techniques. Due to the nature of massive sequence variation in BCR, caution is warranted when interpreting repertoire diversity from error-prone sequencing data. Furthermore, we provide a summary of statistical frameworks and bioinformatics tools for clonal evolution and diversity. Finally, we discuss limitations of current BCR-seq technologies and future perspectives on advances in repertoire sequencing.

A Study on the Curvilinear Relationship Between Slack and Innovation : Focus on Moderating Effect of Network Diversity (조직의 여유자원과 혁신간의 비선형관계에 관한 연구 : 네트워크 다양성 조절효과)

  • Kang, Sora;Han, Su Jin
    • Journal of Information Technology Applications and Management
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    • v.27 no.6
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    • pp.181-196
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    • 2020
  • Based on the resource-based perspective, this study seeks to understand the relationship between the organizational slack and innovation, and to demonstrate that there exists a difference in the influence of the organizational slack according to the type of innovation by dividing the types of innovation into exploratory and exploitative innovations. They also want to understand the role that network diversity plays in the relationship between organizational slack and innovation. For this purpose, hypothesis and research models were presented based on resource-based perspectives and empirical analysis was conducted on 171 companies. The analysis confirmed that the impact of organizational slack on exploitative innovation is linear, not non-linear, as expected. In other words, the more resources available, the more productive the enterprise is, and the more resources available to the organization have a positive impact on the innovation. On the other hand, exploratory innovation represented an inverse U-shaped relationship between organizational slack and nonlinearity as expected. The control effect of network diversity was only seen in the relationship between organizational slack and exploratory innovation. Through this study, it provides implications such as the importance of network diversity, which is a relationship between companies, and the difference in the utilization of organizational slack according to the type of innovation.

Leadership as a Driver of Employees' Innovation Performance: The Mediating Effect of Cultural Diversity in UAE Universities

  • ALMASKARI, Tariq Humaid;MOHAMAD, Effendi;YAHAYA, Siti Norbaya;JALIL, Muhammad Farhan
    • The Journal of Asian Finance, Economics and Business
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    • v.8 no.8
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    • pp.271-285
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    • 2021
  • The aim of this research was to look into the relationship between leadership; transformational leadership, transactional leadership, and employee innovation, as well as the mediating impact of cultural diversity between leadership and employee innovation. Structured questionnaire was used to collect the data from 633 public and private universities' employees in the United Arab Emirates (UAE) with the help of the stratified sampling technique, and hypothesis verified through structural equations modelling (AMOS-21). Findings of the study shows that leadership has positive impact on employee innovation and cultural diversity partially mediates the relationship between leadership and innovative performance of UAE universities' employees. Practical implication of the study is to understand how universities can enhance their employees' innovation which is crucial for their competitiveness and survival. Moreover, the increasing prevalence of cultural diversity, as work arrangements in universities, raises the question of how to successfully manage employees. Although few studies have looked into how transformational and transactional leadership styles affect employees' innovation performance, this study expands on the topic by concentrating on sub-dimensions of leadership that foster innovation through idea generation and execution at the United Arab Emirates universities. This study offers valuable insights for educational leaders and throws light on the main characteristics of leadership which helps the employees to perform better in terms of innovation.

Genetic Diversity of Amylomyces rouxii from Ragi tapai in Java Island Based on Ribosomal Regions ITS1/ITS2 and D1/D2

  • Delva, Ega;Arisuryanti, Tuty;Ilmi, Miftahul
    • Mycobiology
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    • v.50 no.2
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    • pp.132-141
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    • 2022
  • Amylomyces rouxii is commonly found as amylolytic fungi in tapai fermentation. However, its diversity is rarely reported despite being often used for food production in Southeast Asia. This research aims to analyze the genetic diversity and the distribution pattern of A. rouxii from Ragi tapai in Java Island, Indonesia. We isolated the fungus from samples obtained from Ragi tapai producing centers in Bandung, Sumedang, Muntilan, Blora, Yogyakarta, and Bondowoso. The obtained isolates were molecularly identified based on the ribosomal regions ITS1/ITS2 and D1/D2, then analyzed for phylogenetic tree reconstruction, genetic distance, genetic variation, and haplotype networking. Six isolates showed specific morphological traits of A. rouxii. However, phylogenetic tree reconstruction on the ribosomal genes showed that the isolates were grouped into two different clades related to two species. Clade A included BDG, SMD, and MTL isolates related to A. rouxii, whereas clade B included YOG, BLR, and BDS isolates related to Mucor indicus. The genetic distances between clades for ITS1/ITS2 and D1/D2 were 0.6145 and 0.1556, respectively. In conclusion, we confirmed the genetic diversity of molds from Ragi tapai in Java Island and showed that the isolates are not only related to A. rouxii as reported before.

Knee-driven many-objective sine-cosine algorithm

  • Hongxia, Zhao;Yongjie, Wang;Maolin, Li
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.17 no.2
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    • pp.335-352
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    • 2023
  • When solving multi-objective optimization problems, the blindness of the evolution direction of the population gradually emerges with the increase in the number of objectives, and there are also problems of convergence and diversity that are difficult to balance. The many- objective optimization problem makes some classic multi-objective optimization algorithms face challenges due to the huge objective space. The sine cosine algorithm is a new type of natural simulation optimization algorithm, which uses the sine and cosine mathematical model to solve the optimization problem. In this paper, a knee-driven many-objective sine-cosine algorithm (MaSCA-KD) is proposed. First, the Latin hypercube population initialization strategy is used to generate the initial population, in order to ensure that the population is evenly distributed in the decision space. Secondly, special points in the population, such as nadir point and knee points, are adopted to increase selection pressure and guide population evolution. In the process of environmental selection, the diversity of the population is promoted through diversity criteria. Through the above strategies, the balance of population convergence and diversity is achieved. Experimental research on the WFG series of benchmark problems shows that the MaSCA-KD algorithm has a certain degree of competitiveness compared with the existing algorithms. The algorithm has good performance and can be used as an alternative tool for many-objective optimization problems.