• Title/Summary/Keyword: Research Diversity

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Molecular Phylogeny and Morphology Reveal the Underestimated Diversity of Mortierella (Mortierellales) in Korea

  • Lee, Jae-Sung;Nam, Bora;Lee, Hyang Burm;Choi, Young-Joon
    • The Korean Journal of Mycology
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    • v.46 no.4
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    • pp.375-382
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    • 2018
  • Members of the genus Mortierella (Mortierellales) are filamentous fungi, which are found on nearly all substrates, but more frequently in soil. Till date, 7 species of Mortierella have been reported in Korea, but being a ubiquitous group with high species diversity in temperate zones, this number is still low. During a survey of fungal biodiversity in Korea, we collected many isolates of Mortierella, and through morphological and molecular phylogenetic analyses, identified them to be 3 previously unrecorded species, namely, M. chienii, M. epicladia, and M. gamsii. A total of 10 Mortierella species in Korea, including the 3 species reported in the present study, are widely distributed in 5 out of 7 phylogenetic groups of this genus. This indicates that the diversity of Mortierella was so far underestimated in Korea. Multi-locus sequence analysis is required to provide a more reliable backbone for some uncertain phylogenetic groupings and to more clearly define a species of Mortierella, which would encourage deeper research in the diversity and ecological roles of Mortierella and allied genera.

A Research Synthesis on Mathematics Education for Students with Diversity Including Multicultural Education, Language Minority, and Social Economic Status (다양성 배경을 지닌 학생들의 학습현장에서 수학교육연구에 관한 문헌고찰)

  • ChoiKoh, Sang-Sook
    • Journal of the Korean School Mathematics Society
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    • v.12 no.4
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    • pp.389-409
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    • 2009
  • This article was to investigate the previous research as a research synthesis in the area of Mathematics Education for students with diversity including multi-cultural education, language minority, and social economic status. The following summaries were made: Recognizing equity in students with diversity; Restoring teachers' perspectives toward poststandardization; Introducing creative curricular based on students' characteristics; Application of the direct instruction; Foci on interests, challenges and mastery learning; Application of Anchored Instruction; Application of CRA; Tasks, tools, & classroom norms; Enhancement of connection and communication using small-group activity; Development of programs enriched by bilingual education; and Producing curriculum for students from North Korea.

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Phylogenetic Analysis of Culturable Arctic Bacteria

  • Lee, Yoo-Kyung;Kim, Hyo-Won;Cho, Kyeung-Hee;Kang, Sung-Ho;Lee, Hong-Kum;Kim, Yea-Dong
    • Ocean and Polar Research
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    • v.26 no.1
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    • pp.51-58
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    • 2004
  • We isolated and identified culturable Arctic bacteria that had inhabited soils around the Korean Arctic Research Station Dasan located at Ny-Alsund, Svalbard, Norway $(79^{\circ}N,\;12^{\circ}E)$. The collected soils were diluted in distilled water; the diluted soil-water was spread on 3M petri-films at Dasan Station. The petri-films were transported to the laboratory at KORDI, and cultured at $4^{\circ}C$. Colonies grown on the petri-films were subsequently cultured on nutrient agar plates at $4^{\circ}C$ every 7 days. The pure colonies were inoculated into nutrient liquid media, genomic DNA was extracted, and phylogenetic analysis was performed on the basis of 165 rDNA sequences. A total of 227 strains of bacteria were isolated. Among them, 16S rDNA sequences of 185 strains were identical with those of known strains isolated in this study, and 42 strains were finally identified. Phylogenetic analysis using 16S rDNA indicated that the 30 strains belonged to Pseudomonas, 7 strains to Arthrobacter, two strains to Flavobacterium, and the remaining to Achromobacter, Pedobacter, and Psychrobacter. Among the 42 strains, 14 bacteria produced protease: they were 6 strains of Pseudomonax, 4 strains of Arthrobater, an Achromobacter strain, 2 strains of Flavobacterium, and a Pedohacter strain. We expect these Arctic bacteria can be used for screening to develop new industrial enzymes that are active at low temperatures.

Mass Spectrometry-Based Metabolite Profiling and Bacterial Diversity Characterization of Korean Traditional Meju During Fermentation

  • Lee, Su Yun;Kim, Hyang Yeon;Lee, Sarah;Lee, Jung Min;Muthaiya, Maria John;Kim, Beom Seok;Oh, Ji Young;Song, Chi Kwang;Jeon, Eun Jung;Ryu, Hyung Seok;Lee, Choong Hwan
    • Journal of Microbiology and Biotechnology
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    • v.22 no.11
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    • pp.1523-1531
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    • 2012
  • The metabolite profile of meju during fermentation was analyzed using mass spectrometry techniques, including GC-MS and LC-MS, and the bacterial diversity was characterized. The relative proportions of bacterial strains indicated that lactic acid bacteria, such as Enterococcus faecium and Leuconostoc lactis, were the dominant species. In partial least-squares discriminate analysis (PLS-DA), the componential changes, which depended on fermentation, proceeded gradually in both the GC-MS and LC-MS data sets. During fermentation, lactic acid, amino acids, monosaccharides, sugar alcohols, and isoflavonoid aglycones (daidzein and genistein) increased, whereas citric acid, glucosides, and disaccharides decreased. MS-based metabolite profiling and bacterial diversity characterization of meju demonstrated the changes in metabolites according to the fermentation period and provided a better understanding of the correlation between metabolites and bacterial diversity.

The Genetic Diversity of Trans-caucasian Native Sheep Breeds

  • Hirbo, Jibril;Muigai, Anne;Naqvi, A.N.;Rege, E.D.;Hanotte, Olivier
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.7
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    • pp.943-952
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    • 2006
  • The genetic variation in 10 indigenous Caucasian sheep breeds was studied with 14 micro-satellite loci in order to determine the genetic diversity among and between the breeds. Five breeds from Asia, five breeds from Europe and one breed from Africa, were included in order to study any relationships or influences they may have with the Caucasian sheep analyzed. A Karakul population from Uzbekistan was included in the study to see whether there was any Central Asian influence. All the 14 loci were found to be polymorphic in all the breeds, with the exception of ILST0056, which was monomorphic in Imeretian. A total of 231 alleles were generated from all the 688 individuals of the sheep analyzed. The mean number of alleles (MNA) at each locus was 16.5. The total number of alleles detected in all samples ranged from 13 in several loci to 23 in OarJMP029. Out of total 308 Hardy-Weinberg Equilibrium (HWE) tests, 85 gave significant results. After Bonferroni correction for multiple tests, 30 comparisons still remained significant to the experimental levels. The Gala population was the most diverse and Imeretian the least diverse with a MNA of 8.50 and 5.51, respectively. Gene diversity estimates exhibited the same trend and ranged from 0.803 in Gala and 0.623 in Imeretian, but generally there is higher diversity among the Caucasian breeds in comparison to other eference breeds. The closest breeds were Tushin and Bozakh with Da of 0.113 and most distant breeds were $Djallonk{\acute{e}}$ and North Rondalsy with Da of 0.445. Principal Component (PC) analyses were done. PC1 described 14% of the differences. PC2, which described 13% of the differences, further separated the Caucasian breeds from Asian breeds except Karakul and Awasi, and the two British breeds. PC3 described 10% of the differences, allowing better differentiation of the Caucasian breeds. A moderate degree of reliability was observed for individual-breed assignment from the 14 loci using different approaches among which the Bayesian method proved to be the most efficient. About 72% of individuals analyzed were correctly assigned to their respective breeds.

A Method Of Compound Noun Phrase Indexing for Resolving Syntactic Diversity (구문 다양성 해소를 위한 복합명사구 색인 방법)

  • Cho, Min-Hee;Jeong, Do-Heon
    • The Journal of the Korea Contents Association
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    • v.11 no.3
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    • pp.467-476
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    • 2011
  • Compound noun phrase (CNP) is important factor for semantic information process because the meaning of the CNP is more disambiguous than that of single word. However, the CNP can be expressed in various types even though it expresses same meaning. It is called syntactic diversity. It makes information system difficult to grasp sense identity. In order to resolve the syntactic diversity in this research, we propose an indexing method for compound noun phrase. The main purpose is to make identical index term for various types of CNPs which has same meaning. To do so, the research follows next steps. For the first, we make rule template and utilize the template to extract CNPs from set of domestic research papers. In general, the CNP has a unique meaning. Considering the characteristic, we suggest synthesis rules of index terms and apply the rule to CNPs extracted in previous step. For the objective performance evaluation of the research, a test set, HANTEC 2.0, was utilized and the result was compared to baseline model. Through the experiment and the evaluation, we have confirmed that the indexing method suggested in this paper could positively affect retrieval precision and improve performance of the information retrieval.

Genetic Diversity and Population Genetic Structure of Cephalotaxus koreana in South Korea

  • Hong, Kyung Nak;Kim, Young Mi;Park, Yu Jin;Lee, Jei Wan
    • Korean Journal of Plant Resources
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    • v.27 no.6
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    • pp.660-670
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    • 2014
  • The Korean plum yew (Cephalotaxus koreana Nakai) is a shade-tolerant, coniferous shrub. The seeds have been used as a folk medicine in Korea, and an alkaloid extract (HTT) is known to have anticancer properties. We estimated the genetic diversity of 429 trees in 16 populations in South Korea using 194 polymorphic amplicons from seven combinations of AFLP primer-restriction enzymes. The average number of effective alleles and the percentage of polymorphic loci were 1.37 and 79.4%, respectively. Shannon's diversity index and the expected heterozygosity were 0.344 and 0.244, respectively. We divided 16 populations into four groups on the UPGMA dendrogram and the PCA biplot. The first two principal components explained 84% of the total genetic variation. Genetic differentiation between populations explained 14% of total genetic variation, and the remaining 86% came from difference between individuals within populations, as determined by an analysis of molecular variance (AMOVA). However, the genetic differentiation did not correlate with the geographic distance between populations from the Mantel test. The Bayesian statistics, which are comparable to Wright's $F_{ST}$ and Nei's $G_{ST}$, were ${\theta}^I=0.406$ and ${\theta}^{II}=0.172$, respectively. The population genetic diversity was slightly lower, and the strength of genetic differentiation was much weaker, than the average of those plants having similar life histories, as assessed using arbitrary marker systems. We discuss strategies for the genetic conservation of the plum yew in Korea.

Island-Biogeographical Characteristics of Insular Flora in Southern Sea of Jeollanamdo, Korea (전라남도 남해안 도서식물상의 도서생물 지리학적 특성)

  • Kim, Hyun Hee;Kim, Da Bin;Won, Hyun Kyu;Kim, Chan Soo;Kong, Woo Seok
    • Journal of Climate Change Research
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    • v.7 no.2
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    • pp.143-155
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    • 2016
  • Present work aims to establish the countermeasure for the better maintenance and preservation of insular floristic diversity at the South Sea of Jeollanamdo Province, ROK, where unique ecosystems are under threat due to climate change, anthropogenic disturbance and habitat destruction. Numerous flora reports from 15 inhabited islands and 60 uninhabited islands as well as field survey data are collated for the compilation of floristic data base and island biogeographical analysis. Out of the 1,940 vascular plant species from 180 families occurring in studies areas, 30.1 percentage or 584 plant species are physiognomically belonging to arboreal plants. Average number of species at individual island is numbered about 222 species, but it varies from about 591 species at the inhabited islands to 129 species at the uninhabited islands. Only 0.15 percentage of species with high proportion above 0.9 in its relative occurrence rate occurs at 68 islands and it includes three species, such as Pinus thunbergii, Eurya japonica and Pteridium aquilinum var. latiusculum. However, about 68 percentage of plant species occurring in study area is confined their distribution to less than seven islands. Presence of high proportion of notable plants in small islands, i.e., 10 critically endangered species compared with 5.5 species in average, 9 endangered species (average 4.2 spp.) at Sonjookdo, and 7 critically endangered species, 8 endangered species at Sokomundo may due to existence of diverse geological and topographical environmental diversity as well as lower human population density and remoteness from the mainland. Since island is small in size and geographically isolated, minor environmental and ecological burdens can cause the critical damages to the diversity of flora and vegetation, urgent island biogeographical research is needed for the scientific conservation and management of island biodiversity.

Genetic diversity and population genetic structure of Cambodian indigenous chickens

  • Ren, Theary;Nunome, Mitsuo;Suzuki, Takayuki;Matsuda, Yoichi
    • Animal Bioscience
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    • v.35 no.6
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    • pp.826-837
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    • 2022
  • Objective: Cambodia is located within the distribution range of the red junglefowl, the common ancestor of domestic chickens. Although a variety of indigenous chickens have been reared in Cambodia since ancient times, their genetic characteristics have yet to be sufficiently defined. Here, we conducted a large-scale population genetic study to investigate the genetic diversity and population genetic structure of Cambodian indigenous chickens and their phylogenetic relationships with other chicken breeds and native chickens worldwide. Methods: A Bayesian phylogenetic tree was constructed based on 625 mitochondrial DNA D-loop sequences, and Bayesian clustering analysis was performed for 666 individuals with 23 microsatellite markers, using samples collected from 28 indigenous chicken populations in 24 provinces and three commercial chicken breeds. Results: A total of 92 haplotypes of mitochondrial D-loop sequences belonging to haplogroups A to F and J were detected in Cambodian chickens; in the indigenous chickens, haplogroup D (44.4%) was the most common, and haplogroups A (21.0%) and B (13.2%) were also dominant. However, haplogroup J, which is rare in domestic chickens but abundant in Thai red junglefowl, was found at a high frequency (14.5%), whereas the frequency of haplogroup E was considerably lower (4.6%). Population genetic structure analysis based on microsatellite markers revealed the presence of three major genetic clusters in Cambodian indigenous chickens. Their genetic diversity was relatively high, which was similar to findings reported for indigenous chickens from other Southeast Asian countries. Conclusion: Cambodian indigenous chickens are characterized by mitochondrial D-loop haplotypes that are common to indigenous chickens throughout Southeast Asia, and may retain many of the haplotypes that originated from wild ancestral populations. These chickens exhibit high population genetic diversity, and the geographical distribution of three major clusters may be attributed to inter-regional trade and poultry transportation routes within Cambodia or international movement between Cambodia and other countries.

Assessment of population structure and genetic diversity of German Angora rabbit through pedigree analysis

  • Abdul Rahim;K. S. Rajaravindra;Om Hari Chaturvedi;S. R. Sharma
    • Animal Bioscience
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    • v.36 no.5
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    • pp.692-703
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    • 2023
  • Objective: The main goals of this investigation were to i) assess the population structure and genetic diversity and ii) determine the efficiency of the ongoing breeding program in a closed flock of Angora rabbits through pedigree analysis. Methods: The pedigree records of 6,145 animals, born between 1996 to 2020 at NTRS, ICAR-CSWRI, Garsa were analyzed using ENDOG version 4.8 software package. The genealogical information, genetic conservation index and parameters based on gene origin probabilities were estimated. Results: Analysis revealed that, 99.09% of the kits had both parents recorded in the whole dataset. The completeness levels for the whole pedigree were 99.12%, 97.12%, 90.66%, 82.49%, and 74.11% for the 1st, 2nd, 3rd, 4th, and 5th generations, respectively, reflecting well-maintained pedigree records. The maximum inbreeding, average inbreeding and relatedness were 36.96%, 8.07%, and 15.82%, respectively. The mean maximum, mean equivalent and mean completed generations were 10.28, 7.91, and 5.51 with 0.85%, 1.19%, and 1.85% increase in inbreeding, respectively. The effective population size estimated from maximum, equivalent and complete generations were 58.50, 27.05, and 42.08, respectively. Only 1.51% of total mating was highly inbred. The effective population size computed via the individual increase in inbreeding was 42.83. The effective numbers of founders (fe), ancestors (fa), founder genomes (fg) and non-founder genomes (fng) were 18, 16, 6.22, and 9.50, respectively. The fe/fa ratio was 1.12, indicating occasional bottlenecks had occurred in the population. The six most influential ancestors explained 50% of genes contributed to the gene pool. The average generation interval was 1.51 years and was longer for the sire-offspring pathway. The population lost 8% genetic diversity over time, however, considerable genetic variability still existed in the closed Angora population. Conclusion: This study provides important and practical insights to manage and maintain the genetic variability within the individual flock and the entire population.