• Title/Summary/Keyword: Research Diversity

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대청호 퇴적층으로부터 분리된 방선균의 속 다양성 (Genus Diversity of Actinomycetes Isolated from the Sediments in Lake Daechung)

  • 박동진;육영수;박해룡;박철;이상화;김창진
    • 한국미생물·생명공학회지
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    • 제27권4호
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    • pp.273-277
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    • 1999
  • From each sediments collected at Chudong(static) and Hoenam(streaming) site of Lake Daechung on May 18(before rainy season) and on August 24 in 1998(after rainy season), different strains of Actinomycetes were isolated and identified to genus level. For comparison, the genus diversity of Actinomycetes in control soils such as grass land around lake, lake shore, and shallow(5-10cm) lake was also investigated. In consequence, the isolation of Streptomyces from the sediments of Lake Daechung was the most copious(41 strains) at the streaming site before rainy season and the least(27 strains) at the static site before rainy season, which were 2.4- and 1.6-fold larger than the average(17 strains) of control soils, respectively. In addition, the isolation of rare Actinomycetes from the sediments of Lake Daechung was the most copious(23 strains) at the streaming site before rainy season and the least(12 strains) at the static site after rainy season, which were 3.2-and 1.6-fold larger than the average(17 strain) of controls, respectively. Therefore, it is considered that lake sediments exhibit higher diversity of Streptomycete as well as rare Actinomycetes than general soils, and thereby can be utilized as useful sources to isolate diverse Actinomycetes.

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cpcBA-Intergenic Spacer Region을 이용한 Cyanobacteria의 다양성 분석 (Cyanobacterial Diversity Analysis Using cpcBA-Intergenic Spacer Region)

  • 최강국;박용하;안치용;배명숙;오희목
    • 미생물학회지
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    • 제41권4호
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    • pp.287-292
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    • 2005
  • 대청호에서 수화 발생이 빈번한 추소리 수역에서 2005년 3월 15일에 채취한 시료를 대상으로 유전자 분식에 의한 cyanobacteria의 다양성을 조사하였다. rpcBA-Intergenic Spacer (IGS)는 cyanobacteria에 특징적 색소인 phycocyanin을 합성하는 유전자와 유전자 사이의 부분으로, 환경시료에서 cyanobacteria의 다양성을 조사하기에 매우 유용한 기능 유전자이다. cpcBA-IGS를 이용하여 restriction fragment length polymorphism (RELP)으로 cyanobacteria의 다양성을 분석한 결과 Phomidium 속은 58 clones, Anabaena 속은 14 clones, Microcyxtis 속은 4 clones, Spirulina 속은 1 clone 그리고 uncultured cyanobacteria 2 clones가 존재하였다. 전반적으로 Phormidium 속이 우점하였으며, 여름철에 수화를 일으키는 Anabaena 속과 Microcystis 속도 많이 분포하였다. 따라서 cyanobacteria는 cpcBA-IGS와 같은 기능 유전자에 의한 종 동정 및 군집분석이 가능함을 보였다.

사상체질의 Cholecystokinin 유전자 단일염기다형성 연구 (Genetic Polymorphism of CCK Promotor Region and Sasang Constitution)

  • 이수경;이성진
    • 대한한의학회지
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    • 제25권3호
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    • pp.105-110
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    • 2004
  • Objectives : Sasang Constitutional Medicine is based on the diversity of human beings and medically developing a variation of responses to diseases and medicines. This diversity is categorized into four concerning morphology, physiology, pathology, and pharmacology. It is supposed that sasang constitutional medicine is related the genetic diversity of individuals. Single nucleotide polymorphism is the basic tool to research genetic polymorphism as a landmark of genomes. Each constitution has different processes of pathophysiology and metabolisms to herb medications. In clinical research, the stroke incidence is significantly different by constitution. Methods : We researched whether the polymorphic expression of CCK (rs=2241997) depends on sasang constitution. The [c/t] polymorphism site of promotor region of CCK gene on 3p22-p21.3 was investigated. Results : The allele frequency of [c/t] polymorphism of CCK promotor region was different in constitution groups compared to the average allele frequency of SNP DB. The allele frequencies of Soeumin and Soyangin groups were (c:0.70/t:0.30). and (c:0.71/t:0.29), that of Taeumin group was (c:0.57/t:0.43) and of Taeyangin group was (c:1.00/t:0.00) Conclusions : It was regarded the [c/t] polymorphism of CCK promotor region is available to classify the constitution. However, it is necessary to research about CCK gene polymorphism and more constitution population groups. It is also necessary to research the more functional gene's polymorphism and sasang constitution.

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Genetic Diversity of Common Reed in Korea Based on Morphological Characteristics and Random Amplified Polymorphic DNA Markers

  • Chu, Hyo-Sub;Cho, Won-Kyong;Rim, Yeong-Gil;Jo, Yeon-Hwa;Kim, Jae-Yean
    • 한국자원식물학회지
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    • 제24권6호
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    • pp.666-674
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    • 2011
  • To elucidate genetic diversity of common reed in Korea, we collected a total of 674 common reed plants from 27 regions in South Korea. Hierarchical clustering using 7 morphological traits divided the 27 common reed populations into 7 groups. Random amplified polymorphic DNA (RAPD) results identified three distinct groups of common reed. Common reed accessions in group I mostly inhabit coastal areas. Group II includes reeds mostly collected from inland areas. Group III consists of common reed accessions collected from inland and coastal areas, suggesting that this group might contain hybrids. In summary, we suggest that parapatric speciation might be an important factor in the genetic diversity of common reed and geographical speciation of common reed that might be also affected by environmental gradients.

SRAP 분석에 의한 중국 재배삼의 유전적 다양성 (Genetic Diversity and Genetic Structures in Ginseng Landraces (Cultivars) by SRAP Analysis)

  • ;;김영창;방경환;차선우
    • 한국약용작물학회지
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    • 제18권3호
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    • pp.180-185
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    • 2010
  • We investigated genetic diversity among and within the populations of cultivated ginseng (Panax ginseng C. A. Meyer ) using SRAP profiles. A total of 24 ginseng plants were sampled from the three populations (two from China, one from Korea). Since all these populations are previously shown closely related to each other assister groups, we used Panax quinquefolium L. and wild ginseng as a reference species, which is not "within the sister group". All individuals from the three populations were screened with a total of 36 primer pairs with 26 primers generated from 328 SRAP bands of DNA gels. The mean gene diversity ($H_E$) was estimated to be 0.057 within populations (range 0.032-0.067), and 0.086 at the species level. The genetic differentiation (Gst=0.31) indicates that genetic variation apportioned 30% among populations and 70% within populations. Generally, the result of this study indicates that ginseng contains high molecular variation in its populations.

Assessment of Genetic Diversity and Population Structure on Kenyan Sunflower (Helianthus annus L.) Breeding Lines by SSR Markers

  • Mwangi, Esther W.;Marzougui, Salem;Sung, Jung Suk;Bwalya, Ernest C.;Choi, Yu-Mi;Lee, Myung-Chul
    • 한국자원식물학회지
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    • 제32권3호
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    • pp.244-253
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    • 2019
  • In crop breeding program, information about genetic dissimilarity on breeding resources is very important to corroborate genealogical relationships and to predict the most heterozygotic hybrid combinations and inbred breeding. This study aimed to evaluate the genetic variation in Kenyan sunflower breeding lines based on simple sequence repeat (SSR). A total of 83 alleles were detected at 32 SSR loci. The allele number per locus ranged from 2 to 7 with an average of 2.7 alleles per locus detected from the 24 sunflower accessions and the average value of polymorphic information contents (PIC) were 0.384. A cluster analysis based on the genetic similarity coefficients was conducted and the 24 sunflower breeding resources were classified into three groups. The principal coordinates (PCoA) revealed 34% and 13.38% respectively, and 47.38% of total variation. It was found that the genetic diversity within the Kenyan sunflower breeding resources was narrower than that in other sunflower germplasm resources, suggesting the importance and feasibility of introducing elite genotypes from different origins for selection of breeding lines with broader genetic base in Kenyan sunflower breeding program.

Antecedents of Duty Free Shop Willingness to Pay

  • Song, Myungkeun;Moon, Joonho;Tang, Ruo-Han
    • 아태비즈니스연구
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    • 제12권1호
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    • pp.87-100
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    • 2021
  • Purpose - This study aims to examine the antecedents of willingness to pay in the domain of duty free shop. Design/methodology/approach - This study chooses willingness to pay as explained variable. The candidates of explanatory variables are price fairness, brand awareness, employee service, product diversity, and crowding. This study uses survey to explore the linear relation between variables. This research collected data using online panel data collection service. The number of valid observation is 265. The research targe is Lotte duty free store. Statistical analysis was for statistical inference. To attain the information of survey respondents, frequency analysis is employed. Next, this study implemented exploratory factor analysis and reliability to ensure both validity and reliability of measurement items. This study executed multiple regression analysis to test research hypotheses Findings - Regrading results, brand awareness, employee service, and product diversity are positively associated with willingness to pay of duty free shop product. Moreover, the results of regression analysis suggest the inverted-U shape association between crowding and willingness to pay. However, price fairness appeared as non-significant variable to account for willingness to pay in the multiple regression analysis. Originality - This study contributes to the literature by examining duty free shop customers more.

유튜브 관광콘텐츠 특성이 몽골인의 관광지 선택에 미치는 영향 (The influence of tourism content characteristics of YouTube on destination visit intentions)

  • 바트바타르 바트자야;김영국
    • 아태비즈니스연구
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    • 제14권2호
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    • pp.141-151
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    • 2023
  • Purpose - This study aims to determine whether four characteristics of tourism content on YouTube (i.e., entertainment; credibility; diversity; and informativeness) influence tourists' destination choices. Design/methodology/approach - A total of 201 Koreans and Mongolians who use YouTube were surveyed in the research. Validity and reliability, correlation, and regression analyze were performed in SPSS 29.0. Findings - As a result of the study, it was found that the features of YouTube tourism content, such as entertainment, credibility, and diversity, have a positive effect on tourists' choice of destination. Also, it was found that there is no difference in the perception of tourism content features between Korean and Mongolian YouTube users and it is not statistically significant. Research implications or Originality - Tourists' YouTube content shows that YouTube features such as entertainment, variety, and trustworthiness, influencing tourists' travel destination choices. The limitations of this study and suggestions for future research were discussed.

백합나무 시험림(試驗林)의 모의간벌(模擬間伐)에 따른 유전다양성(遺傳多樣性) 변화(變化) (Changes in Genetic Diversity of a Test Plantation of Liriodendron tulipifera L. by simulated Practices for Seed Trees)

  • 홍용표;류근옥;조경진;홍경락
    • 한국산림과학회지
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    • 제90권1호
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    • pp.155-160
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    • 2001
  • 경기도 화성군 반월면 속달리 소재의 백합나무 종자 산지 시험림을 대상으로 모수림 작업 전후의 유전다양성의 변화를 예측하기 위해서 총 305개체에 대한 I-SSR 표지자 분석을 수행하였다. 9개의 I-SSR primer를 이용하여 PCR을 수행한 결과 총 89개의 증폭산물 변이 체를 확인했으며, 속달리 시험림에 식재되어 있는 전 개체에 대한 다양도는 0.4532로 비교적 높은 수치를 보였다. 종자산지별로 구분하여 분석한 결과 미국 채종원산 종자를 양묘하여 식재한 개체군에서 가장 높은 다양도(0.4530)를 보였으며, 그 다음이 안양 산지로 0.4152, 전북 산지의 경우 0.3929로 가장 낮은 다양도를 보였다. 식재목의 형태적 특성과 식재 간격을 고려하여 두 번에 걸친 간벌을 수반하는 모수림 작업 결과 남겨질 37개 개체목에 대한 유전다양도 및 유전적 거리를 모의 통계분석한 결과, 종자 산지내 다양도는 전북이 28.3%로 가장 크게 감소했으며, 그 다음이 안양으로 16.3%, 미국이 8.0%로 가장 적게 감소한 것으로 나타났다. 개체목 감소율의 차이가 적었음에도 불구하고(87.5~88.2%) 다양도의 변화에 큰 차이가 나는 이유는 미국산 채종원 종자는 비교적 다수의 모수로부터 유래되었으나 안양 및 전북에서 생산된 종자들은 상대적으로 소수의 모수로부터 유래되었을 가능성이 크기 때문인 것으로 추정된다. 비록 2차에 걸친 간벌에 의해서 각 종자 산지내 개체간 다양도는 평균 17.5%가 감소했으나, 전체 37개체에 대한 다양도의 감소는 불과 6.1%에 지나지 않기 때문에 속달리 시험림에 식재되어 있는 305개체 중에서 종자생산을 목적으로 본 연구에서 선정한 기준에 따라 37개체를 잔존시키는 모수림 작업을 수행하더라도 이들로부터 생산될 차대들에 있어서 전체적인 유전다양성의 현격한 감소를 초래하지는 않을 것으로 생각된다.

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