• Title/Summary/Keyword: Research Diversity

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Sampling and Selection Factors that Enhance the Diversity of Microbial Collections: Application to Biopesticide Development

  • Park, Jun-Kyung;Lee, Seung-Hwan;Lee, Jang-Hoon;Han, Songhee;Kang, Hunseung;Kim, Jin-Cheol;Kim, Young Cheol;McSpadden Gardener, Brian
    • The Plant Pathology Journal
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    • v.29 no.2
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    • pp.144-153
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    • 2013
  • Diverse bacteria are known to colonize plants. However, only a small fraction of that diversity has been evaluated for their biopesticide potential. To date, the criteria for sampling and selection in such bioprospecting endeavors have not been systematically evaluated in terms of the relative amount of diversity they provide for analysis. The present study aimed to enhance the success of bioprospecting efforts by increasing the diversity while removing the genotypic redundancy often present in large collections of bacteria. We developed a multivariate sampling and marker-based selection strategy that significantly increase the diversity of bacteria recovered from plants. In doing so, we quantified the effects of varying sampling intensity, media composition, incubation conditions, plant species, and soil source on the diversity of recovered isolates. Subsequent sequencing and high-throughput phenotypic analyses of a small fraction of the collected isolates revealed that this approach led to the recovery of over a dozen rare and, to date, poorly characterized genera of plant-associated bacteria with significant biopesticide activities. Overall, the sampling and selection approach described led to an approximately 5-fold improvement in efficiency and the recovery of several novel strains of bacteria with significant biopesticide potential.

Political Diversity and Participation: A Systematic Review of the Measurement and Relationship

  • Jun, Najin
    • Asian Journal for Public Opinion Research
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    • v.1 no.2
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    • pp.103-127
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    • 2014
  • This study reviews existing research on the measurement of and the relationship between political diversity and political participation. It addresses the inconsistency in the arguments of existing studies researching the influence of political diversity on political participation. It attempts to find the cause in the variety of approaches to conceptualize and operationalize the two variables. As the measure of political diversity, political network heterogeneity and network attributes are discussed in detail in specific relation to political participation. As for political participation, an in-depth analysis of various ways to understand different forms of political involvement is presented. Implications for public opinion research are discussed.

Genetic Variation in the Selected Populations of Hovenia dulcis var. koreana Nakai. Based on RAPD Analysis

  • Kim Sea-Hyun;Han Jin-Gyu;Chung Hun-Gwan;Cho Yoon-Jin;Park Hyung-Soon
    • Plant Resources
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    • v.8 no.3
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    • pp.293-299
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    • 2005
  • This study used RAPD markers to assume genetic diversity and variation in selected populations of Hovenia dulcis var. koreana. Ratio of polymorphic RAPD markers were 93.4% in selected populations of Hovenia dulcis Thunb., difference of genetic structure among populations and within populations showed 16.45%, 83.55%, respectively in amount of total genetic variation of 4 populations. Total gene diversity($H_T$) that show genetic diversity appeared 0.313 and coefficient of gene differentiation($G_{ST}$) that compare genetic differentiation of populations appeared 0.1645, analysis of AMOVA for variation among populations and within populations was significantly different (P<0.001). Genetic diversity of whole populations showed that 12.44% difference among population and 87.56% difference within populations. As a result, difference within populations was larger than difference among populations in genetic diversity. Nei's genetic distance and cluster analysis appeared that mean genetic distance among populations was 0.076, thus dividing two main groups and geographic relationship did not show in populations.

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Genetic Variability Comparison of Wild and Cultured Far Eastern Catfish (Silurus asotus) of Korea using Microsatellite Marker

  • Kim, Jung Eun;Hwang, Ju-Ae;Kim, Hyeong Su;Lee, Jeong-Ho
    • Development and Reproduction
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    • v.24 no.4
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    • pp.317-325
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    • 2020
  • The Far Eastern catfish (Silurus asotus) is an important commercial freshwater fish in Korea. Investigation of the genetic diversity of wild and cultured domestic catfish groups is essential for the restoration of fishery resources and for increasing local revenue. However, there are relatively few genetic diversity studies on wild and cultured catfish in Korea. In the present study, we analyzed the genetic diversity and association of wild and cultured catfish using five microsatellite markers. We determined that the number of alleles per locus (NA) ranged from 9 to 25, wherein the Jeonbuk catfish demonstrated the highest mean number of alleles per locus and the cultured catfish exhibited the lowest. The average expected heterozygosity (He) of the wild catfish samples was 0.907, and that of the cultured catfish showed was 0.875. The genetic distances (GD value) among populations of all catfish ranged from 0.138 to 0.242. Jeonnam and Jeonbuk wild catfish were located closest to each other, and the cultured group was separated from the other groups. In conclusion, the present study confirmed that the genetic diversity of wild and cultured catfish was maintained at a high level. In the case of the wild group, it is effective in maintaining diversity due to the continuous fry release by the local fish research institute. However, the genetic diversity of cultured catfish declined. Low diversity is associated with slow growth and weakened immunity, and therefore continuous monitoring is necessary.

Comparative Analysis of the Korean Population of Magnaporthe oryzae by Multilocus Microsatellite Typing

  • Choi, Jaehyuk;Kim, Hyojung;Lee, Yong-Hwan
    • The Plant Pathology Journal
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    • v.29 no.4
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    • pp.435-439
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    • 2013
  • Rice blast fungus, Magnaporthe oryzae, inflicts serious damage to global rice production. Due to high variability of this fungal pathogen, resistance of newly-released rice cultivars is easily broken down. To understand the population structure of M. oryzae, we analyzed the genetic diversity of the Korean population using multilocus microsatellite typing. Eleven microsatellite markers were applied to the population of 190 rice isolates which had been collected in Korea for two decades since the 1980's. Average values of gene diversity and allele frequency were 0.412 and 6.5, respectively. Comparative analysis of the digitized allele information revealed that the Korean population exhibited a similar level of allele diversity to the integrated diversity of the world populations, suggesting a particularly high diversity of the Korean population. Therefore, these microsatellite markers and the comprehensive collection of field isolates will be useful genetic resources to identify the genetic diversity of M. oryzae population.

The Effect of Informational Diversity-Sensitive Orientation on Employee Attitudes : The Moderating Effects of Supervisory Communication & Co-Worker Communication (정보다양성 수용 정도가 종업원 태도에 미치는 영향 : 상사소통 및 동료소통의 조절효과)

  • Jeong, Hyeon-Dal;Baek, Yoon-Jung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.7
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    • pp.569-578
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    • 2017
  • The purpose of this study is to investigate the effects of informational diversity-sensitive orientation on employee attitudes, focusing on the moderating effects of communication. Recently, diversity within organizations has intensified and the importance of diversity management within organizations is increasing. Diversity studies have focused on the variance of objective diversity characteristics, and have provided mixed results. In order to overcome limitations in the research, this study focuses on members' perception of diversity. To this end, the variable of informational diversity-sensitive orientation(IDSO) was used. Sample data is derived from 301 surveys of employees in firms located in South Korea. Results show that informational diversity-sensitive orientation(IDSO) had a positive effect on job satisfaction and organizational commitment. Regarding the moderating effect of communication, a positive moderating effect was exhibited only in co-worker communication, and differential influence derived from communication type. Based on the analysis results, we discuss research implications and directions for future research.

R&D Project Team Diversity and Team Performance : Investigating the Moderating Effect of Innovative Problem Solving Style and Interdependence (R&D 프로젝트 팀 다양성과 팀 성과간의 관계: 제해결스타일 및 상호의존성의 조절효과)

  • Park, Owwon
    • Journal of Korea Technology Innovation Society
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    • v.16 no.4
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    • pp.913-936
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    • 2013
  • As diversity has getting its significance, recent research on diversity has moved the focus from direct effects of diversity on performance to moderating effects between the two. In this paper, I analyzed the moderating effects of the innovative problem solving style and three types of interdependence (i.e., task, goal and outcome) on the relationship between diversity and performance. Empirical results using 75 project teams of R&D institute showed that the innovative problem solving style positively strengthened the effect of diversity on performance. All three types of interdependence also amplified the relationship between diversity and performance. I addressed theoretical and practical implications, and future research directions.

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The Effects of Perception Levels of Diversity on Team Performance: Moderating Effects of Conflict (다양성 지각 정도가 팀의 성과에 미치는 영향: 갈등의 조절효과를 중심으로)

  • Yun, Jun Hee;Shin, Ho-Chul
    • Journal of Korean Society for Quality Management
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    • v.41 no.2
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    • pp.289-300
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    • 2013
  • Purpose: The results of prior studies investigating main effects between diversity and team performance are neither clear nor consistent. This study seeks to provide more evidence for a direct link between these two. The present study also attempts to close a gap in the literature by examining conflict as a moderator of the relationship between diversity and team performance. Methods: As suggested by Williams and O'Reilly(1998), this study examines the effects of perception levels of three specific types of diversity (social category diversity, value diversity, and informational diversity) and two contextual moderators (task conflict and relationship conflict) on team performance. Results: The results show that perception levels of all three types of diversity are significantly related to team performance in the current sample of 313. Results also indicate that relationship conflict significantly interacted with the perception levels of diversity in relating to team performance. Results for task conflict, however, fails to support the hypothesis. Conclusion: The results suggest that the effects of diversity on team functioning depend on how diversity is perceived by team members, rather than actual diversity seen by team members. Theoretical and practical implications are reviewed, and limitations of the study and suggestions for future research are addressed.

Comparative Enzyme Production by Fungi from Diverse Lignocellulosic Substrates

  • Sin, Marie K.W.;Hyde, Kevin D.;Pointing, Stephen B.
    • Journal of Microbiology
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    • v.40 no.3
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    • pp.241-244
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    • 2002
  • Fungi commonly encountered on monocotyledonous substrates were evaluated for their in vitro ability to produce enzymes involved in lignocellulose breakdown. Most were capable of structural polysac-charide utilization, but few produced enzymes associated with lignin breakdown. None of the mono-cotyledon-inhabiting fungi produced reactions as strongly as wood decay fungi.

Isolation and inheritance of microsatellite loci for the oily bittering (Acheilognathus koreensis): applications for analysis of genetic diversity of wild populations

  • Kim, Woo-Jin;Kong, Hee-Jeong;Shin, Eun-Ha;Kim, Chi-Hong;Kim, Hyung-Soo;Kim, Young-Ok;Nam, Bo-Hye;Kim, Bong-Seok;Lee, Sang-Jun;Jung, Hyung-Taek
    • Animal cells and systems
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    • v.16 no.4
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    • pp.321-328
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    • 2012
  • The oily bittering Acheilognathus koreensis is a freshwater species that is endemic to Korea and is experiencing severe declines in natural populations as a result of habitat fragmentation and water pollution. For the conservation and restoration of this species, it is necessary to assess its genetic diversity at the population level. We developed 13 polymorphic microsatellite loci that were used to analyze the genetic diversity of two populations collected from the Kum River and the Tamjin River in Korea. All loci exhibited Mendelian inheritance patterns when examined in controlled crosses. Both populations revealed high levels of variability, with the number of alleles ranging from 3 to 20 and observed and expected heterozygosities ranging from 0.500 to 0.969 and from 0.529 to 0.938, respectively. None of the loci showed significant deviation from Hardy-Weinberg equilibrium, and one pair of loci showed significant linkage disequilibrium after Bonferroni correction. Pairwise $F_{ST}$ and genetic distance estimation showed significant differences between two populations. These results suggest that the microsatellites developed herein can be used to study the genetic diversity, population structure and conservation measure of A. koreensis.