• Bulletin of the Korean Chemical Society
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• v.32 no.10
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• pp.3650-3654
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• 2011

Two new unusual soyasaponins named 6"-O-methyldehydrosoyasaponin I(7) and desglucosylsoyasaponin $A_1$ (10) along with eight known saponins, dehydrosoyasaponin IV (1), dehydrosoyasaponin III (= impatienoside A) (2), soyasaponin III (3), dehydrosoyasaponin II (= soyasaponin Bg) (4), soyasaponin II (5), dehydrosoyasaponin I (= soyasaponin Be) (6), soyasaponin I (8), and kudzusaponin $SA_3$ (9), were isolated as their methyl esters and identified from the liquid culture of G. applanatum. Their structures were determined by chemical and spectroscopic analyses including 1D- and 2D-NMR as well as by comparison of their spectroscopic data with those of the reported in literatures. Although dehydrosoyasaponin IV was identified by LC-MS/MS method from soy protein isolate, this is the first report of the isolation of this compound. Dehydrosoyasaponin III (2) and kudzusaponin $SA_3$ (9) were also isolated for the first time from soybean. The presence of soyasaponins in Ganoderma species seems to be unusual feature. Thus, we presumed that compounds 1-10 might all be derived from the defatted soybean flour which was added to the culture medium as a nitrogen source.

• The Research Journal of the Costume Culture
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• v.29 no.2
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• pp.289-306
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• 2021

The study aims to define the Korean Wave as global attention to Korea's unique culture and consider the specificity of traditional Korean fashion images in Korean Wave content. The research method of this study is a case study through literature research. In order to collect Korean Wave content on YouTube, 24 channels with the highest number of views were selected from among content uploaded from 2018 to the present through keyword search, and up to two channels with high views showing traditional Korean fashion images. As a result of selecting the analysis target, 41 Korean Wave videos and 368 costumes were selected and analyzed based on fashion style elements, including item, color, detail, motif, styling, silhouette, and accessory. As a result of the study, music, broadcast, fashion, and other content were found in the Korean Wave content fields in which Korean fashion style appeared, and the characteristics of each field were derived. Music content was characterized by fashion style excluding stereotypes about traditional Korean costume, broadcast and fashion content was characterized by fashion style inherited from traditional costume, and other content was characterized by symbolic fashion style of traditional culture. This study is meaningful in revealing the formative characteristics of traditional Korean design elements recently shared online through the study of Korean traditional fashion images in Korean Wave content.

• International Journal of Advanced Culture Technology
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• v.12 no.2
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• pp.16-29
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• 2024

Mogao Grottoes in Dunhuang, China is one of the world cultural heritage, is the largest ancient grottoes art group in the world, and is a natural and cultural place with outstanding universal value for mankind all over the world. Dunhuang music and dance derived from the murals of Mogao Grottoes is an artistic representation of its thousand-year-old glorious history and an indispensable historical material for research. As one of the iconic images of Dunhuang music and dance, the "rebound lute behind the back" dance posture has unique charm value both in the original mural composition and the stage image. This paper analyzes the characters holding pipa in Dunhuang murals by case analysis, comparative research and other relevant research methods, then studies the stage image and posture of "rebound lute behind the back", and finally analyzes the stage works of "rebound lute behind the back". It is concluded that the dance image of "rebound lute behind the back" is a dynamic stage art work gradually formed by artists from the static Dunhuang murals through refining, developing, processing and transforming. This is to revive the image of Dunhuang music and dance murals, to provide reference and reference for the inheritance and development of Dunhuang culture, and then to enhance and enrich the artistic value of excellent traditional Chinese culture and world cultural heritage.

• Architectural research
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• v.26 no.3
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• pp.63-72
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• 2024

Due to budgetary constraints, national landmarks symbolic of country's power and cultural stature face significant limitations in their creation. Within the current total project cost management system, there are considerable restrictions on constructing cultural facilities with various forms and designs, and there exists a societal need for a design-first administrative system for public buildings that demand artistry and symbolism. This study explores the potential and limitations of applying exceptions to the total project cost management system through the designation of representative artistic architecture. It discusses directions for the implementation of government-level systems. To this end, a literature review on budget management and national project management was conducted, and an analysis of related policy cases both do-mestically and internationally was performed to select applicable examples and consider their implications for policy utilization within the domestic context. The results discuss the necessity of public art building construction, the issues with construction budgets, and the in-volvement of world-class architects. Analytical implications include the need for realistic budget setting for construction projects, establishing clear promotion criteria, and creating a phased process and expert evaluation panel.

• Development and Reproduction
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• v.20 no.1
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• pp.63-71
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• 2016

Human embryonic stem cells (hESCs) have been routinely cultured on mouse embryonic fibroblast feeder layers with a medium containing animal materials. For clinical application of hESCs, animal-derived products from the animal feeder cells, animal substrates such as gelatin or Matrigel and animal serum are strictly to be eliminated in the culture system. In this study, we performed that SNUhES32 and H1 were cultured on human amniotic fluid cells (hAFCs) with KO-SR XenoFree and a humanized substrate. All of hESCs were relatively well propagated on hAFCs feeders with xeno-free conditions and they expressed pluripotent stem cell markers, alkaline phosphatase, SSEA-4, TRA1-60, TRA1-81, Oct-4, and Nanog like hESCs cultured on STO or human foreskin fibroblast feeders. In addition, we observed the expression of nonhuman N-glycolylneuraminic acid (Neu5GC) molecules by flow cytometry, which was xenotransplantation components of contamination in hESCs cultured on animal feeder conditions, was not detected in this xeno-free condition. In conclusion, SNUhES32 and H1 could be maintained on hAFCs for humanized culture conditions, therefore, we suggested that new xeno-free conditions for clinical grade hESCs culture will be useful data in future clinical studies.

• Korean Journal of Human Ecology
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• v.13 no.6
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• pp.967-983
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• 2004

The purpose of this study is to review Hanliu phenomenon, a kind of social and cultural phenomenon, in China and to analyze its effects on the fashion style of new young generation of China. In this study, Hanliu phenomenon means the enthusiasm of Asian people for Korean mass culture including Korean dramas, pop songs, and fashions from late 1990s. This research adopts two kinds of methods for analyzing the phenomenon: qualitative and quantitative research methods. As a qualitative research method, we analyzed it with several sources of documentaries and audio-visual materials: articles from newspapers and magazines, special TV reports, and documentary movie files from Internet. As a quantitative research method, we surveyed approximately 100 female students of Beijing university and asked how they feel Korean culture and fashions. The Hanliu phenomenon led to the popularity of Korean products as well as general culture of Korea. Also, it influenced Chinese young generation so much that Korean fashion has become prevailing. Such influence on the street fashion of Chinese youths can be summarized in three factors as follows: First, Korean entertainers' fashion is widely imitated. For example, H.O.T-like hairstyles, hip-hop styles, large heel shoes with boots-cut pants, and long-curled permanent hairstyles have been on among Chinese youths. Second, the preference for Korean fashion products has highly increased. The number of stores dealing with Korean fashion products has increased. Even the 'Kim Hee Seen,' a fashion brand named after a famous Korean actress, was introduced. Finally, Korean culture and products have widely been imitated in China as much as the increasing popularity of Korean fashion products. This study reveals that Hanliu phenomenon is widespread in China, and Chinese youths are largely affected by the fashion styles of Korean entertainers. Also, Korean fashion products are largely imitated and benchmarked in China. Hanliu phenomenon is a big chance to approach the fashion market of China, the largest buying power in the world. To make inroads into the Chinese fashion market, we suggest that we need to have our own brand and to make the most of culture, stars, and Internet in marketing. Also, we need a well-planned strategy for a success in the Chinese fashion market.

• Proceedings of the Korea Contents Association Conference
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• 2007.11a
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• pp.724-726
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• 2007

Many enterprises make use of 'mecenat action' for positive image ensuring to company and harmony within organization members. Also, at the same time the research has continued estimating of efficiency about that area. but that estimations get accomplished inclusive and abstractive, and then estimation of significant effect isn't sufficient. In this study we consider that many enterprises practice culture and art education program, and related that analyze the estimation of effect in 'mecenat action'. Stand on this analysis we attempts to extract factor, having representation character with making standard for estimation of education effect. That is a implication that relation education programs are prepared many enterprises, In future study, we will need to make standard and select methods about effect estimation, add measurable index that reliability can increase.

• International Journal of Oral Biology
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• v.35 no.1
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• pp.1-5
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• 2010

Human fibroblasts that maintain the structural integrity of connective tissues by secreting precursors of the extracellular matrix are typically cultured with serum. However, there are potential disadvantages of the use of serum including unnatural interactions between the cells and the potential for exposure to animal pathogens. To prevent the possible influence of serum on fibroblast cultures, we devised a serum-free growth method and present in vitro data that demonstrate its suitability for growing porcine fetal fibroblasts. These cells were grown under four different culture conditions: no serum (negative control), 10% fetal bovine serum (FBS, positive control), 10% knockout serum replacement (KSR) and 20% KSR in the medium. The proliferation rates and viabilities of the cells were investigated by counting the number of cells and trypan blue staining, respectively. The 10% FBS group showed the largest increase in the total number of cells ($1.09\;{\times}\;10^5\;cells/ml$). In terms of the rate of viable cells, the results from the KSR supplementation groups (20% KSR:64.7%; 10% KSR: 80.6%) were similar to those from the 10% FBS group (68.5%). Moreover, supplementation with either 10% ($3.0\;{\times}\;10^4\;cells/ml$) or 20% KSR ($4.8\;{\times}\;10^4\;cells/ml$) produced similar cell growth rates. In conclusion, although KSR supplementation produces a lower cell proliferation rate than FBS, this growth condition is more effective for obtaining an appropriate number of viable porcine fetal fibroblasts in culture. Using KSR in fibroblast culture medium is thus a viable alternative to FBS.

• ALGAE
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• v.28 no.2
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• pp.193-202
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• 2013

Nitrogen availability and cell density each affects growth and cellular astaxanthin content of Haematococcus pluvialis, but possible combined effects of these two factors on the content and productivity of astaxanthin, especially under outdoor culture conditions, is less understood. In this study, the effects of the initial biomass densities IBDs of 0.1, 0.5, 0.8, 1.5, 2.7, 3.5, and 5.0 g $L^{-1}$ DW and initial nitrogen concentrations of 0, 4.4, 8.8, and 17.6 mM nitrate on growth and cellular astaxanthin content of H. pluvialis Flotow K-0084 were investigated in outdoor glass column photobioreactors in a batch culture mode. A low IBD of 0.1 g $L^{-1}$ DW led to photo-bleaching of the culture within 1-2 days. When the IBD was 0.5 g $L^{-1}$ and above, the rate at which the increase in biomass density and the astaxanthin content on a per cell basis was higher at lower IBD. When the IBD was optimal (i.e., 0.8 g $L^{-1}$), the maximum astaxanthin content of 3.8% of DW was obtained in the absence of nitrogen, whereas the maximum astaxanthin productivity of 16.0 mg $L^{-1}\;d^{-1}$ was obtained in the same IBD culture containing 4.4 mM nitrogen. The strategies for achieving maximum Haematococcus biomass productivity and for maximum cellular astaxanthin content are discussed.

• Journal of Korea Trade
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• v.26 no.1
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• pp.57-80
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• 2022

Purpose - This study aims to examine the relationships between the acceptance of the 4th industrial revolution logistics technology, logistics safety behavior, and logistics safety performance, as well as the moderated mediating effects of logistics safety behavior through safety culture in Korea. Design/methodology - Research models and hypotheses were established based on prior research related to the 4th industrial revolution logistics technology, logistics safety, and logistics performance. The survey was conducted on the employees of logistics companies, and reliability analysis, confirmatory factor analysis, discriminant validity analysis, structural equation model analysis, and mediating effect analysis were performed. In addition, the moderated mediating effect analysis applying SPSS Process Model No. 7 was conducted. Findings - Usefulness and sociality of the acceptance of the 4th industrial revolution logistics technology had a significant effect on logistics safety behavior. Ease of use, sociality, and efficiency had meaningful effect on logistics safety performance. And in the relationships between the acceptance of logistics technology and logistics safety performance, logistics safety behavior had a significant mediating effect. But the moderated mediating effect of safety behavior through safety culture was not significant. Logistics companies can improve logistics safety performance through the utilization of new logistics technologies such as intelligent logistics robots, autonomous driving technology, and artificial intelligence, etc. Originality/value - This is the first study to analyze the relationships between the acceptance of logistics technology in the 4th industrial revolution and logistics safety. In addition, previous studies analyzed mediating effects or moderating effects, but this is the first study to identify the moderated mediating effects of safety behavior through safety culture. In other words, it has originality in terms of research methodology.